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The map label for this gene is thiN [H]

Identifier: 222526553

GI number: 222526553

Start: 4139591

End: 4140241

Strand: Direct

Name: thiN [H]

Synonym: Chy400_3321

Alternate gene names: 222526553

Gene position: 4139591-4140241 (Clockwise)

Preceding gene: 222526552

Following gene: 222526554

Centisome position: 78.57

GC content: 60.52

Gene sequence:

>651_bases
ATGACGTACACAGTGATTGTAGCAAATGCACCGGCGATTGATCTCACGCCGTACCAGCGGTTGCTGACGCTTGCCGACCG
GCTGATTGCCGCAGATGGCGGCGGTACTGCCCTGTTTGCCGGCGGCTACACGCCGCACGTGATCATTGGTGATCTCGACT
CAATTGCTGCGGAAGCGCTGACTGCGTATCAGGCACAAGGGGTCGCGATTGAACGGCATCGCCCCGACAAAGACGAGACC
GATCTCGAACTGGCGTTGCTGGCCGCAGCCGCTATGGGTGCGACCCGGATCGACGTGATCGGGGCACTGGGTGGACGTTG
GGATCAATCGCTGGCGAACGTCATGTTGTTGGGGATGCCGGAGCTGCGTGGCCGCCGGGTTCGTCTGCTCGACGTTCCGC
AACAAATTTGGCTGGTGGATCGTGAGAGTATCATCCCCGGCCTCGTAGGTGATACCGTCTCGTTGCTGCCGTTTGGCGGC
GATGCCCACGGTATCACGACCGCAGGACTGGCCTATCCGCTTACGAATGGAACGCTTTACCATGACCGGGCACGTGGCGT
GAGCAATGTTATCAATAACCTGCCGGCGCGCGTTTCGGTTGGGCAGGGCATGCTGCTAGTGGTACTGACGACGCAGGCTG
AGCCAGTGTAG

Upstream 100 bases:

>100_bases
GCGGGCGGGTGACCGTGCCGGTGTTGCGGGTGATCAATCTGGTGTCAGGGTTGGTGATTATCGGGTTTGGGGTAGCCGCC
TTGTTGAGTAGAGGCCTGTG

Downstream 100 bases:

>100_bases
GGAGTTGGTCTGCGCCGGTGTGCCACAGGCGACGAATAGCCTTGCGTTTTCGAGAGGAATATCGCAATGCTTCTCGACCT
ATCCTCACTGGTTCGCGTGA

Product: thiamine pyrophosphokinase

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MTYTVIVANAPAIDLTPYQRLLTLADRLIAADGGGTALFAGGYTPHVIIGDLDSIAAEALTAYQAQGVAIERHRPDKDET
DLELALLAAAAMGATRIDVIGALGGRWDQSLANVMLLGMPELRGRRVRLLDVPQQIWLVDRESIIPGLVGDTVSLLPFGG
DAHGITTAGLAYPLTNGTLYHDRARGVSNVINNLPARVSVGQGMLLVVLTTQAEPV

Sequences:

>Translated_216_residues
MTYTVIVANAPAIDLTPYQRLLTLADRLIAADGGGTALFAGGYTPHVIIGDLDSIAAEALTAYQAQGVAIERHRPDKDET
DLELALLAAAAMGATRIDVIGALGGRWDQSLANVMLLGMPELRGRRVRLLDVPQQIWLVDRESIIPGLVGDTVSLLPFGG
DAHGITTAGLAYPLTNGTLYHDRARGVSNVINNLPARVSVGQGMLLVVLTTQAEPV
>Mature_215_residues
TYTVIVANAPAIDLTPYQRLLTLADRLIAADGGGTALFAGGYTPHVIIGDLDSIAAEALTAYQAQGVAIERHRPDKDETD
LELALLAAAAMGATRIDVIGALGGRWDQSLANVMLLGMPELRGRRVRLLDVPQQIWLVDRESIIPGLVGDTVSLLPFGGD
AHGITTAGLAYPLTNGTLYHDRARGVSNVINNLPARVSVGQGMLLVVLTTQAEPV

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 22798; Mature: 22666

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYTVIVANAPAIDLTPYQRLLTLADRLIAADGGGTALFAGGYTPHVIIGDLDSIAAEAL
CEEEEEEECCCEECCCHHHHHHHHHHHHEECCCCCEEEEECCCCCEEEEECHHHHHHHHH
TAYQAQGVAIERHRPDKDETDLELALLAAAAMGATRIDVIGALGGRWDQSLANVMLLGMP
HHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHEEEEHHCCCCHHHHHHHEEEECCC
ELRGRRVRLLDVPQQIWLVDRESIIPGLVGDTVSLLPFGGDAHGITTAGLAYPLTNGTLY
CCCCCEEEEEECCHHHEEEEHHHCCCCCCCCCEEEEECCCCCCCEEECCEEEECCCCEEE
HDRARGVSNVINNLPARVSVGQGMLLVVLTTQAEPV
EHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCC
>Mature Secondary Structure 
TYTVIVANAPAIDLTPYQRLLTLADRLIAADGGGTALFAGGYTPHVIIGDLDSIAAEAL
EEEEEEECCCEECCCHHHHHHHHHHHHEECCCCCEEEEECCCCCEEEEECHHHHHHHHH
TAYQAQGVAIERHRPDKDETDLELALLAAAAMGATRIDVIGALGGRWDQSLANVMLLGMP
HHHHHCCEEEECCCCCCCCCHHHHHHHHHHHCCCHHEEEEHHCCCCHHHHHHHEEEECCC
ELRGRRVRLLDVPQQIWLVDRESIIPGLVGDTVSLLPFGGDAHGITTAGLAYPLTNGTLY
CCCCCEEEEEECCHHHEEEEHHHCCCCCCCCCEEEEECCCCCCCEEECCEEEECCCCEEE
HDRARGVSNVINNLPARVSVGQGMLLVVLTTQAEPV
EHHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]