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The map label for this gene is lepA [H]

Identifier: 222526540

GI number: 222526540

Start: 4121361

End: 4123280

Strand: Direct

Name: lepA [H]

Synonym: Chy400_3307

Alternate gene names: 222526540

Gene position: 4121361-4123280 (Clockwise)

Preceding gene: 222526539

Following gene: 222526541

Centisome position: 78.22

GC content: 57.08

Gene sequence:

>1920_bases
GTGTGTCGCCATACCCAACAGCGAGGCCGTGTCTATGGTATACTGCATAGCAGAAACGAAGGGATATTTGCGGTATTGCC
GCCGATTGAAGTACACATGTCAGATCAACTGCACATCCGTAATTTCAGCATCATCGCTCACGTCGATCACGGCAAAACAA
CGCTGAGTGATCGCTTACTTGAAGCAACGCGCACGATCAGCGAGCGTGAACAGCGCGAGCAACTGCTCGACTCGCTCGAT
CTGGAACGCGAAAAAGGGATCACGATCAAGTTAAAGCCGGTGCGCATGGAGTATCGCGCAGCCGATGGTCAGCTTTACCA
GCTTAACCTGATCGATACGCCCGGTCACGTGGATTTTAGCTACGAAGTCAACCGCTCGCTGGCAGCGTGCGAAGGGGCGT
TGCTGGTTGTTGATGCCTCGCAGGGGATTGAGGCGCAGACCCTCGCCAATACCTATCTGGCGCTGGAAAATGATCTGGTG
ATTATTCCGGTCGTCAACAAGATCGACCTGCCCGGTGCGCATCCCGAAGAGGTGGCGCAGGAGATTGAGACGGTGATTGG
TATTCCCGCCGAGGATGCGATCATGGCTTCGGCGAAGGAAGGAATCGGGATTGCCGAGATTCTGGAGGCGATTGTCCGCC
GTATTCCACCGCCACGTGGTCAACGCCAGCAGCCGGCACGGGCGCTCATCTTTGACTCGCACTACGATCCGTACAAAGGT
GTCATTGCCTATGTTCGGGTTGTTGATGGTGCCTTCACCAGTCGTGATCGGGTGCGCTTTATGGGCACCGGTGCCCAGGC
CGAAACCCTCGAACTCGGCATCTTCCGCCCGGCCATGACTCCGCTGAATGAACTGGTTGCCGGTGAAGTGGGCTATATTG
CCACCGGCTTGAAGAGCGTGGCCGACTGTCAGGTGGGTGATACGATCACTCTGGCCGACGCCCCGGCTGCCGAGCCGCTA
CCCGGTTATCGCCCGGCCAAACCGATGGTCTTCGCCGGTCTCTATCCCATCGACTCGAATGCCTATCCTGAGTTGCGTGA
AGCGCTCGAACGTCTGAAACTGAACGATGCGGCGCTCTCGTTTCAGCCGGAAAGCTCGGCGGCGCTGGGATTTGGGTTCC
GCTGCGGATTTCTCGGCCTTTTGCACATGGAGATTGTGCGTGAACGGCTTGAACGCGAGTATAAGCTCGATCTGCTGATC
ACCGCACCCTCGGTCGAATACCAGGTTTTGCTCTCTGATGGCGAGATTGTGACCGTCGCCAATCCATCGGAGATGCCCGA
TCAGTCGCGGGTTGAAGCGATTGAGGAGCCGGTGGTCGAGATTAGTGTGATCGCACCGGTGCGCTATATCGGGACGATTA
TGGAGCTGGTGACGACCCGACGCGGGACGTTCATCAGTATGGATTATCTTGACCCGACACGGGTGCTCTTGAAATATCGG
ATGCCTCTGGCCGAGATCGTGATCGATTTCTACGATCAACTGAAGAGCCGCACGCAAGGTTACGCCTCACTCGATTATCA
TCTGGCCGGCTATCAGGAAGCTGACCTGGTGAAGCTGGATATTCTGGTCAACGGGCAACCGGTTGATGCCCTCTCGCTGA
TTGTGCATCGCGATTTTGCCTACCAGCGTGGTCGTGATCTGGTGGAACGGCTACGGCAGTTGATCCCGCGCCAGATGTTC
GAGGTGCCGATCCAGGCCGCGATTGGCTCGAAGGTGATTGCCCGCGAGACGATCCGCGCCTTGCGCAAGGATGTGCTGGC
GAAGTGTTACGGTGGTGACGTCACGCGCAAACGGAAGCTGCTCGAAAAGCAGAAAGAGGGCAAGAAGCGCATGAAGATGG
TGGGGAGTGTGGAGATTCCCCAGGAAGCCTTCATGGCGATCCTGTCACTCAATGAAACGGGTAGCGAGCGTGAGCGGTAG

Upstream 100 bases:

>100_bases
GTATGAGGTTGCATTGATCGGCAGGACGGCGTCGCTATGCCGGGCTAGAGAGGATCAAGATTGTCGGCTCAATCCTTGTC
GCTCACCTTGAGAAAGTTAG

Downstream 100 bases:

>100_bases
GGTTGTGAGGATGAACGAGGCGAATGTCTATTCGCCTCGTATTTTTGCGAAGGAAGCTGCCTGAGCGGTTCTCTAGCTGG
TAAGCATGTGTGCTGTGTAA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MCRHTQQRGRVYGILHSRNEGIFAVLPPIEVHMSDQLHIRNFSIIAHVDHGKTTLSDRLLEATRTISEREQREQLLDSLD
LEREKGITIKLKPVRMEYRAADGQLYQLNLIDTPGHVDFSYEVNRSLAACEGALLVVDASQGIEAQTLANTYLALENDLV
IIPVVNKIDLPGAHPEEVAQEIETVIGIPAEDAIMASAKEGIGIAEILEAIVRRIPPPRGQRQQPARALIFDSHYDPYKG
VIAYVRVVDGAFTSRDRVRFMGTGAQAETLELGIFRPAMTPLNELVAGEVGYIATGLKSVADCQVGDTITLADAPAAEPL
PGYRPAKPMVFAGLYPIDSNAYPELREALERLKLNDAALSFQPESSAALGFGFRCGFLGLLHMEIVRERLEREYKLDLLI
TAPSVEYQVLLSDGEIVTVANPSEMPDQSRVEAIEEPVVEISVIAPVRYIGTIMELVTTRRGTFISMDYLDPTRVLLKYR
MPLAEIVIDFYDQLKSRTQGYASLDYHLAGYQEADLVKLDILVNGQPVDALSLIVHRDFAYQRGRDLVERLRQLIPRQMF
EVPIQAAIGSKVIARETIRALRKDVLAKCYGGDVTRKRKLLEKQKEGKKRMKMVGSVEIPQEAFMAILSLNETGSERER

Sequences:

>Translated_639_residues
MCRHTQQRGRVYGILHSRNEGIFAVLPPIEVHMSDQLHIRNFSIIAHVDHGKTTLSDRLLEATRTISEREQREQLLDSLD
LEREKGITIKLKPVRMEYRAADGQLYQLNLIDTPGHVDFSYEVNRSLAACEGALLVVDASQGIEAQTLANTYLALENDLV
IIPVVNKIDLPGAHPEEVAQEIETVIGIPAEDAIMASAKEGIGIAEILEAIVRRIPPPRGQRQQPARALIFDSHYDPYKG
VIAYVRVVDGAFTSRDRVRFMGTGAQAETLELGIFRPAMTPLNELVAGEVGYIATGLKSVADCQVGDTITLADAPAAEPL
PGYRPAKPMVFAGLYPIDSNAYPELREALERLKLNDAALSFQPESSAALGFGFRCGFLGLLHMEIVRERLEREYKLDLLI
TAPSVEYQVLLSDGEIVTVANPSEMPDQSRVEAIEEPVVEISVIAPVRYIGTIMELVTTRRGTFISMDYLDPTRVLLKYR
MPLAEIVIDFYDQLKSRTQGYASLDYHLAGYQEADLVKLDILVNGQPVDALSLIVHRDFAYQRGRDLVERLRQLIPRQMF
EVPIQAAIGSKVIARETIRALRKDVLAKCYGGDVTRKRKLLEKQKEGKKRMKMVGSVEIPQEAFMAILSLNETGSERER
>Mature_639_residues
MCRHTQQRGRVYGILHSRNEGIFAVLPPIEVHMSDQLHIRNFSIIAHVDHGKTTLSDRLLEATRTISEREQREQLLDSLD
LEREKGITIKLKPVRMEYRAADGQLYQLNLIDTPGHVDFSYEVNRSLAACEGALLVVDASQGIEAQTLANTYLALENDLV
IIPVVNKIDLPGAHPEEVAQEIETVIGIPAEDAIMASAKEGIGIAEILEAIVRRIPPPRGQRQQPARALIFDSHYDPYKG
VIAYVRVVDGAFTSRDRVRFMGTGAQAETLELGIFRPAMTPLNELVAGEVGYIATGLKSVADCQVGDTITLADAPAAEPL
PGYRPAKPMVFAGLYPIDSNAYPELREALERLKLNDAALSFQPESSAALGFGFRCGFLGLLHMEIVRERLEREYKLDLLI
TAPSVEYQVLLSDGEIVTVANPSEMPDQSRVEAIEEPVVEISVIAPVRYIGTIMELVTTRRGTFISMDYLDPTRVLLKYR
MPLAEIVIDFYDQLKSRTQGYASLDYHLAGYQEADLVKLDILVNGQPVDALSLIVHRDFAYQRGRDLVERLRQLIPRQMF
EVPIQAAIGSKVIARETIRALRKDVLAKCYGGDVTRKRKLLEKQKEGKKRMKMVGSVEIPQEAFMAILSLNETGSERER

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=52.0868113522538, Blast_Score=659, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=42.8571428571429, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI25306283, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI25306287, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI19923640, Length=138, Percent_Identity=42.7536231884058, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI18390331, Length=179, Percent_Identity=32.9608938547486, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=38.255033557047, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=40.9090909090909, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=40.9090909090909, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=40.9090909090909, Blast_Score=90, Evalue=9e-18,
Organism=Homo sapiens, GI217272892, Length=139, Percent_Identity=35.2517985611511, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI217272894, Length=139, Percent_Identity=35.2517985611511, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788922, Length=595, Percent_Identity=55.4621848739496, Blast_Score=680, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=509, Percent_Identity=28.2907662082515, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1789738, Length=165, Percent_Identity=35.7575757575758, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=31.4102564102564, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789559, Length=247, Percent_Identity=28.7449392712551, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789108, Length=163, Percent_Identity=31.2883435582822, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=41.8032786885246, Blast_Score=499, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=465, Percent_Identity=24.7311827956989, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71988811, Length=248, Percent_Identity=31.4516129032258, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=248, Percent_Identity=31.4516129032258, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=144, Percent_Identity=41.6666666666667, Blast_Score=97, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=34.5454545454545, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=140, Percent_Identity=35.7142857142857, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=597, Percent_Identity=47.0686767169179, Blast_Score=542, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=36.8421052631579, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=38.9261744966443, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=38.9261744966443, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=39.2857142857143, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=175, Percent_Identity=36, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706572, Length=598, Percent_Identity=46.1538461538462, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24582462, Length=168, Percent_Identity=35.1190476190476, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI28574573, Length=237, Percent_Identity=32.9113924050633, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221458488, Length=162, Percent_Identity=37.037037037037, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=36.6013071895425, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=36.6013071895425, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=36.6013071895425, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=36.2962962962963, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 71308; Mature: 71308

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRHTQQRGRVYGILHSRNEGIFAVLPPIEVHMSDQLHIRNFSIIAHVDHGKTTLSDRLL
CCCCCCCCCCEEEEEEECCCCEEEEECCEEEECCCCEEEEEEEEEEEECCCCHHHHHHHH
EATRTISEREQREQLLDSLDLEREKGITIKLKPVRMEYRAADGQLYQLNLIDTPGHVDFS
HHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEE
YEVNRSLAACEGALLVVDASQGIEAQTLANTYLALENDLVIIPVVNKIDLPGAHPEEVAQ
EECCCCHHHCCCEEEEEECCCCCCHHHHHHHHEEECCCEEEEEECCCCCCCCCCHHHHHH
EIETVIGIPAEDAIMASAKEGIGIAEILEAIVRRIPPPRGQRQQPARALIFDSHYDPYKG
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHH
VIAYVRVVDGAFTSRDRVRFMGTGAQAETLELGIFRPAMTPLNELVAGEVGYIATGLKSV
HHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHH
ADCQVGDTITLADAPAAEPLPGYRPAKPMVFAGLYPIDSNAYPELREALERLKLNDAALS
HCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEE
FQPESSAALGFGFRCGFLGLLHMEIVRERLEREYKLDLLITAPSVEYQVLLSDGEIVTVA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCEEEEE
NPSEMPDQSRVEAIEEPVVEISVIAPVRYIGTIMELVTTRRGTFISMDYLDPTRVLLKYR
CCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHC
MPLAEIVIDFYDQLKSRTQGYASLDYHLAGYQEADLVKLDILVNGQPVDALSLIVHRDFA
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
YQRGRDLVERLRQLIPRQMFEVPIQAAIGSKVIARETIRALRKDVLAKCYGGDVTRKRKL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LEKQKEGKKRMKMVGSVEIPQEAFMAILSLNETGSERER
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MCRHTQQRGRVYGILHSRNEGIFAVLPPIEVHMSDQLHIRNFSIIAHVDHGKTTLSDRLL
CCCCCCCCCCEEEEEEECCCCEEEEECCEEEECCCCEEEEEEEEEEEECCCCHHHHHHHH
EATRTISEREQREQLLDSLDLEREKGITIKLKPVRMEYRAADGQLYQLNLIDTPGHVDFS
HHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEEEEEEEECCCCEEEEEEEECCCCCEEEE
YEVNRSLAACEGALLVVDASQGIEAQTLANTYLALENDLVIIPVVNKIDLPGAHPEEVAQ
EECCCCHHHCCCEEEEEECCCCCCHHHHHHHHEEECCCEEEEEECCCCCCCCCCHHHHHH
EIETVIGIPAEDAIMASAKEGIGIAEILEAIVRRIPPPRGQRQQPARALIFDSHYDPYKG
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHH
VIAYVRVVDGAFTSRDRVRFMGTGAQAETLELGIFRPAMTPLNELVAGEVGYIATGLKSV
HHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHH
ADCQVGDTITLADAPAAEPLPGYRPAKPMVFAGLYPIDSNAYPELREALERLKLNDAALS
HCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEE
FQPESSAALGFGFRCGFLGLLHMEIVRERLEREYKLDLLITAPSVEYQVLLSDGEIVTVA
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEEEECCCEEEEE
NPSEMPDQSRVEAIEEPVVEISVIAPVRYIGTIMELVTTRRGTFISMDYLDPTRVLLKYR
CCCCCCCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHC
MPLAEIVIDFYDQLKSRTQGYASLDYHLAGYQEADLVKLDILVNGQPVDALSLIVHRDFA
CCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHH
YQRGRDLVERLRQLIPRQMFEVPIQAAIGSKVIARETIRALRKDVLAKCYGGDVTRKRKL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LEKQKEGKKRMKMVGSVEIPQEAFMAILSLNETGSERER
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA