Click here to switch to the map view.

The map label for this gene is pleD [H]

Identifier: 222526537

GI number: 222526537

Start: 4118493

End: 4119467

Strand: Direct

Name: pleD [H]

Synonym: Chy400_3304

Alternate gene names: 222526537

Gene position: 4118493-4119467 (Clockwise)

Preceding gene: 222526536

Following gene: 222526538

Centisome position: 78.17

GC content: 53.33

Gene sequence:

>975_bases
ATGCAGGTTAAAAATGCATATACTATTTTTTTATCCATGACCACAAATCGTTCACCTGTCATTCTCGTCGTTGATGACAC
TCCGGCGAATCTGCTGTTGCTCACCCAGCTTCTGAGTGCGCAGTCTTACGATGTGCGCCCGGTCACTCAGGGGACAGCGG
CCTTAACGGCAATCCGCACCATTCGCCCCGATCTCGTCTTGCTTGATATTCGGATGCCGGATATGGATGGTTACGAGGTT
TGCCAGCAACTGAAAGCGGATGAGGAAACAGCGCAGATACCGGTGATCTTCATTAGCGCACTGGATGACGTTGAAGATAA
AGTACGGGCATTTGAGTGTGGCGGGGTGGATTATATTGCAAAGCCGTTTCAACCTCTCGAAGTACTGGCACGGGTGCGCA
CCCACCTGGCGCTGGCCCAGGCGCGCAAAGCTCTCGAAGCTGCCAATCGCGAACTCCAACGAGCATTGGCCCGTGAAGCT
GAGCTGGCCCGGATGGATTGGCTGACCGGCGTGTATAATCGGAGCCATTTCTACGAGCTGGCCCGCCACGAGTTTGCGAT
TGCTACCCGTTATGAACGACCACTGACGATGTGTATGCTCGATGTTGATCACTTTAAGCAGATTAATGATCGCTATGGTC
ATCTGGTTGGTGATCAGGTCTTACGGGCGGTTGCTCAAACTGTCGCGCACCAGATACGCACTGCCGATAGCATCGGTCGT
TTTGGGGGAGAGGAATTCATTCTGTTGTTGCCGAACACGAATCTCCCTTCTGCACTTACGCTGGCCGAACGCTTGCGGGT
AACGATTGCCGATTTACAGGTTCACACCGATAGGGGCATTGTCACAGCAACGATCAGTGTCGGCGTGGCTGAACGCCAGT
CAACTGATACCACGATTGAGCGTTTAATTGATCGCGCCGATCGGGCATTGTACGCTGCGAAACGGCAAGGGCGTAACTGT
GTGGTCGGGGTGTAA

Upstream 100 bases:

>100_bases
GCAGCTCCGCAACTGGATCGAGACGTTCGATTACGAGGCAGTGCGACAGTGGCTTGACCGGTTGGTTTCTTCTCAAACGG
TGGATCGTGTTGTATAGTAT

Downstream 100 bases:

>100_bases
GAGTCTGCTCCCACTCCTCTTATCCTTCCTCACCGAGGTGCTGAAAACAAGGAGAACGCGCATGACAATGCCGGTCATTG
AACTCCACGACCTTACGGTC

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MQVKNAYTIFLSMTTNRSPVILVVDDTPANLLLLTQLLSAQSYDVRPVTQGTAALTAIRTIRPDLVLLDIRMPDMDGYEV
CQQLKADEETAQIPVIFISALDDVEDKVRAFECGGVDYIAKPFQPLEVLARVRTHLALAQARKALEAANRELQRALAREA
ELARMDWLTGVYNRSHFYELARHEFAIATRYERPLTMCMLDVDHFKQINDRYGHLVGDQVLRAVAQTVAHQIRTADSIGR
FGGEEFILLLPNTNLPSALTLAERLRVTIADLQVHTDRGIVTATISVGVAERQSTDTTIERLIDRADRALYAAKRQGRNC
VVGV

Sequences:

>Translated_324_residues
MQVKNAYTIFLSMTTNRSPVILVVDDTPANLLLLTQLLSAQSYDVRPVTQGTAALTAIRTIRPDLVLLDIRMPDMDGYEV
CQQLKADEETAQIPVIFISALDDVEDKVRAFECGGVDYIAKPFQPLEVLARVRTHLALAQARKALEAANRELQRALAREA
ELARMDWLTGVYNRSHFYELARHEFAIATRYERPLTMCMLDVDHFKQINDRYGHLVGDQVLRAVAQTVAHQIRTADSIGR
FGGEEFILLLPNTNLPSALTLAERLRVTIADLQVHTDRGIVTATISVGVAERQSTDTTIERLIDRADRALYAAKRQGRNC
VVGV
>Mature_324_residues
MQVKNAYTIFLSMTTNRSPVILVVDDTPANLLLLTQLLSAQSYDVRPVTQGTAALTAIRTIRPDLVLLDIRMPDMDGYEV
CQQLKADEETAQIPVIFISALDDVEDKVRAFECGGVDYIAKPFQPLEVLARVRTHLALAQARKALEAANRELQRALAREA
ELARMDWLTGVYNRSHFYELARHEFAIATRYERPLTMCMLDVDHFKQINDRYGHLVGDQVLRAVAQTVAHQIRTADSIGR
FGGEEFILLLPNTNLPSALTLAERLRVTIADLQVHTDRGIVTATISVGVAERQSTDTTIERLIDRADRALYAAKRQGRNC
VVGV

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=164, Percent_Identity=42.6829268292683, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786584, Length=154, Percent_Identity=40.2597402597403, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI87082007, Length=161, Percent_Identity=38.5093167701863, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=38.150289017341, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787262, Length=171, Percent_Identity=36.8421052631579, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787802, Length=178, Percent_Identity=34.2696629213483, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=35.2201257861635, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=32.9479768786127, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788394, Length=134, Percent_Identity=34.3283582089552, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787056, Length=128, Percent_Identity=35.9375, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=32.9608938547486, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788550, Length=137, Percent_Identity=35.036496350365, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788381, Length=173, Percent_Identity=30.0578034682081, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI87082012, Length=116, Percent_Identity=37.9310344827586, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=36.2068965517241, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=33.6283185840708, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=29.2397660818713, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790863, Length=138, Percent_Identity=28.9855072463768, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=32.7586206896552, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=103, Percent_Identity=38.8349514563107, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=116, Percent_Identity=34.4827586206897, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790299, Length=113, Percent_Identity=33.6283185840708, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789149, Length=104, Percent_Identity=35.5769230769231, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=111, Percent_Identity=34.2342342342342, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1786911, Length=116, Percent_Identity=33.6206896551724, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=117, Percent_Identity=34.1880341880342, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 36240; Mature: 36240

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKNAYTIFLSMTTNRSPVILVVDDTPANLLLLTQLLSAQSYDVRPVTQGTAALTAIRT
CCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
IRPDLVLLDIRMPDMDGYEVCQQLKADEETAQIPVIFISALDDVEDKVRAFECGGVDYIA
CCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
KPFQPLEVLARVRTHLALAQARKALEAANRELQRALAREAELARMDWLTGVYNRSHFYEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ARHEFAIATRYERPLTMCMLDVDHFKQINDRYGHLVGDQVLRAVAQTVAHQIRTADSIGR
HHHHHHHHHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGGEEFILLLPNTNLPSALTLAERLRVTIADLQVHTDRGIVTATISVGVAERQSTDTTIE
CCCCEEEEEECCCCCCHHHHHHHHHHEEEEEEEEECCCCEEEEEEEECCCCCCCCHHHHH
RLIDRADRALYAAKRQGRNCVVGV
HHHHHHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MQVKNAYTIFLSMTTNRSPVILVVDDTPANLLLLTQLLSAQSYDVRPVTQGTAALTAIRT
CCCCCEEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
IRPDLVLLDIRMPDMDGYEVCQQLKADEETAQIPVIFISALDDVEDKVRAFECGGVDYIA
CCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
KPFQPLEVLARVRTHLALAQARKALEAANRELQRALAREAELARMDWLTGVYNRSHFYEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ARHEFAIATRYERPLTMCMLDVDHFKQINDRYGHLVGDQVLRAVAQTVAHQIRTADSIGR
HHHHHHHHHHCCCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGGEEFILLLPNTNLPSALTLAERLRVTIADLQVHTDRGIVTATISVGVAERQSTDTTIE
CCCCEEEEEECCCCCCHHHHHHHHHHEEEEEEEEECCCCEEEEEEEECCCCCCCCHHHHH
RLIDRADRALYAAKRQGRNCVVGV
HHHHHHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA