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The map label for this gene is punA [H]

Identifier: 222526399

GI number: 222526399

Start: 3925678

End: 3926499

Strand: Direct

Name: punA [H]

Synonym: Chy400_3165

Alternate gene names: 222526399

Gene position: 3925678-3926499 (Clockwise)

Preceding gene: 222526398

Following gene: 222526400

Centisome position: 74.51

GC content: 60.22

Gene sequence:

>822_bases
ATGTATAACGATATCGAACAGGCTTGCGCAGCCATCGTCCCGCGGCTGCGCCTCTCGCCACGGGTTGGCCTCATTCTCGG
TTCCGGCTTGAGTGCGCTGGCCGACGAGATGACTGATGCGGTTGTGATTCCGTACCGTGACATTCCCGGCTTTCACGAGC
CAAAAGTCGTTGGTCATCGCGGTGAGCTGGCGATTGGGATGCTGGCCGGTCAACCGGTAGCGGCGATGCGTGGCCGGTTT
CATTTCTACGAAGGCTACAGCATGCAGGAGGTAACCTTTCCGGTGCGGGTGCTGCGTGCCCTCGGCTGCGAGACGCTGAT
CGTTACCAATGCTGCCGGTGGTTTACGGCCCGACTGGCAGGTTGGGGATATTATGCGGATCAGCGACCAGATTTTTCTGC
CGGGGATGGCCGGCCATCATCCACTGCGCGGCCCAAACGACGAGCGGCTGGGGCCGCGGTTTCCGGCGATGGTGGGCGCC
TTCGATAATGATCTGGTGCCGCTGACCCGCGCAGTAGCAGCCGATCTGGGCATCACGTTGCGCGAGGGTGTCTATTGTAT
GCTGAGTGGCCCTCATTTCGAGAGTGCTGCCGAGTTGCGGATGCTGCGCACATTGGGAGCCGATGCGGTGGGGATGTCAA
CTGCGCCGGAGATTATTGTGGCAATACACGGCGGAATGCGGGTATTGGGCTTTTCCCTGATAACGAATCTGGCCTTGCCA
GATGGCCCACCGGCTAACCACGAAGAGGTGATTGAAGCCGGTGAAGCGGCCAAACCGAAATTTGCCGCTCTGCTGCGCGG
TATCCTGGCTCGGATGGAGTAA

Upstream 100 bases:

>100_bases
TTGAGGCGAAGAAACGCGATCTGCTCAGCCGTATTTGATTTATTGTTCCGGTGATGTTACTGATCCCGTCAGGATCGGTG
TAGATAGAGACAGGTGGTCA

Downstream 100 bases:

>100_bases
TGTTGCCGGATGACGAGACCCAATTGCCCGGCACGGCTTGCCCGGAAGATTAATAATAGAGATGGGAATTTGCTATGCGC
ACACTCACACCATTGACCGC

Product: inosine guanosine and xanthosine phosphorylase family protein

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MYNDIEQACAAIVPRLRLSPRVGLILGSGLSALADEMTDAVVIPYRDIPGFHEPKVVGHRGELAIGMLAGQPVAAMRGRF
HFYEGYSMQEVTFPVRVLRALGCETLIVTNAAGGLRPDWQVGDIMRISDQIFLPGMAGHHPLRGPNDERLGPRFPAMVGA
FDNDLVPLTRAVAADLGITLREGVYCMLSGPHFESAAELRMLRTLGADAVGMSTAPEIIVAIHGGMRVLGFSLITNLALP
DGPPANHEEVIEAGEAAKPKFAALLRGILARME

Sequences:

>Translated_273_residues
MYNDIEQACAAIVPRLRLSPRVGLILGSGLSALADEMTDAVVIPYRDIPGFHEPKVVGHRGELAIGMLAGQPVAAMRGRF
HFYEGYSMQEVTFPVRVLRALGCETLIVTNAAGGLRPDWQVGDIMRISDQIFLPGMAGHHPLRGPNDERLGPRFPAMVGA
FDNDLVPLTRAVAADLGITLREGVYCMLSGPHFESAAELRMLRTLGADAVGMSTAPEIIVAIHGGMRVLGFSLITNLALP
DGPPANHEEVIEAGEAAKPKFAALLRGILARME
>Mature_273_residues
MYNDIEQACAAIVPRLRLSPRVGLILGSGLSALADEMTDAVVIPYRDIPGFHEPKVVGHRGELAIGMLAGQPVAAMRGRF
HFYEGYSMQEVTFPVRVLRALGCETLIVTNAAGGLRPDWQVGDIMRISDQIFLPGMAGHHPLRGPNDERLGPRFPAMVGA
FDNDLVPLTRAVAADLGITLREGVYCMLSGPHFESAAELRMLRTLGADAVGMSTAPEIIVAIHGGMRVLGFSLITNLALP
DGPPANHEEVIEAGEAAKPKFAALLRGILARME

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=276, Percent_Identity=48.9130434782609, Blast_Score=263, Evalue=1e-70,
Organism=Escherichia coli, GI1788746, Length=269, Percent_Identity=43.1226765799257, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17541190, Length=278, Percent_Identity=41.726618705036, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323238, Length=274, Percent_Identity=44.5255474452555, Blast_Score=219, Evalue=3e-58,
Organism=Drosophila melanogaster, GI24656090, Length=257, Percent_Identity=45.9143968871595, Blast_Score=230, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24656093, Length=257, Percent_Identity=45.9143968871595, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45552885, Length=257, Percent_Identity=45.9143968871595, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=43.2727272727273, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=37.6569037656904, Blast_Score=159, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29280; Mature: 29280

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNDIEQACAAIVPRLRLSPRVGLILGSGLSALADEMTDAVVIPYRDIPGFHEPKVVGHR
CCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCEEEEECCCCCCCCCCCEECCC
GELAIGMLAGQPVAAMRGRFHFYEGYSMQEVTFPVRVLRALGCETLIVTNAAGGLRPDWQ
CCEEEEEECCCCHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
VGDIMRISDQIFLPGMAGHHPLRGPNDERLGPRFPAMVGAFDNDLVPLTRAVAADLGITL
CCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCH
REGVYCMLSGPHFESAAELRMLRTLGADAVGMSTAPEIIVAIHGGMRVLGFSLITNLALP
HCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
DGPPANHEEVIEAGEAAKPKFAALLRGILARME
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYNDIEQACAAIVPRLRLSPRVGLILGSGLSALADEMTDAVVIPYRDIPGFHEPKVVGHR
CCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCEEEEECCCCCCCCCCCEECCC
GELAIGMLAGQPVAAMRGRFHFYEGYSMQEVTFPVRVLRALGCETLIVTNAAGGLRPDWQ
CCEEEEEECCCCHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
VGDIMRISDQIFLPGMAGHHPLRGPNDERLGPRFPAMVGAFDNDLVPLTRAVAADLGITL
CCCEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCH
REGVYCMLSGPHFESAAELRMLRTLGADAVGMSTAPEIIVAIHGGMRVLGFSLITNLALP
HCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
DGPPANHEEVIEAGEAAKPKFAALLRGILARME
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]