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The map label for this gene is phoB [H]

Identifier: 222526101

GI number: 222526101

Start: 3531663

End: 3532607

Strand: Direct

Name: phoB [H]

Synonym: Chy400_2858

Alternate gene names: 222526101

Gene position: 3531663-3532607 (Clockwise)

Preceding gene: 222526100

Following gene: 222526102

Centisome position: 67.03

GC content: 52.7

Gene sequence:

>945_bases
GTGAGCAAAGAACGAATCCTCGTCGTTGAGGACCAGCCAGACATCTCCAGCCTGTTGAAAATCTACTTCACTGCTCAGGG
GTATGAAGTCTACACTGCTCTACGCGGTCAACAGGCACTTGAGATTTGCAGCAAAACGCCACCAAATCTGGCCCTGCTCG
ATGTCAATCTCCCGGATATGGAGGGGTATGACGTTGGACGTGCCCTTCGCGCCAGCCCACGTACCCGCCATATTCCAATC
ATCTTCCTGACAGCGCGTGGTGAACGACGTGATCGGCTGATCGGATTGGGTGAAGTACAGGCTCAGTATTACATCGTTAA
ACCCTTCGATATCGAAGAGGTGCACACGATTGTCAAGAACCAGCTCGAAGAGGCACGTCGCCGTAATCAGTTGCATCCGG
TAACCAATCTACCAACGGCTGAGTTGCTGAATGACCAGCTTCGTCAGCTCCTGGTGGCACCGCGCTGGGCACTGATCAAT
GTTCATATCAATGGGTTTGAGACGTTTACGAACGTGTACGGCGCGGTGGTTGGCGAAGATGTGCTGCGCTTCGTTGCCCT
GATGCTGAACGAAGTGGTGAGTGAGCTGGGTGGTAACGAAGAATTTGTTGGTCAACAATCGGTTGGTCAGTCGTTCATGA
TCACGACGCTTCCTGAACGAAGCCACGCTATCTGTGAACGCATCATTGCTCGCTTTGATGAAGAGATCGGTTTGCATTAT
AACTATCGGCATCGTAAACAGGGTTATATCGAGTTTGTTGACGACACCGGTGAAACCCGCCAGGCACCGTTAATGTCGCT
CTCCATTGGGGTTCTGACCCAGGATGATGGCCCATTTGCCGACATTCGCGAGCTGAGTGAAGTGGCTGAGGAGGTGCGCC
AGCGGGCACTCATTCAAGCCCGTGAGCAAGGTAAACGCAGCTTTGCTATCTATGGTCGCTCCTGA

Upstream 100 bases:

>100_bases
GTTCGCTTCGTTCCAATCAGTCTGCCGGGAATGGCCGCCTGCCCGATCACATCAGTGCGAGCCAGCGGTTATAGCCGCCG
TTTCGCGAGGAGAAATCGCT

Downstream 100 bases:

>100_bases
TCAGGGGCCGGCATCCATTACCAAATGTCGATCTGCGTCTCGTTTATGCATGCGAAGTAGTCCTCAGAGCAGAATAGACA
CGCCAGCAGGAGCGATAGTG

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSKERILVVEDQPDISSLLKIYFTAQGYEVYTALRGQQALEICSKTPPNLALLDVNLPDMEGYDVGRALRASPRTRHIPI
IFLTARGERRDRLIGLGEVQAQYYIVKPFDIEEVHTIVKNQLEEARRRNQLHPVTNLPTAELLNDQLRQLLVAPRWALIN
VHINGFETFTNVYGAVVGEDVLRFVALMLNEVVSELGGNEEFVGQQSVGQSFMITTLPERSHAICERIIARFDEEIGLHY
NYRHRKQGYIEFVDDTGETRQAPLMSLSIGVLTQDDGPFADIRELSEVAEEVRQRALIQAREQGKRSFAIYGRS

Sequences:

>Translated_314_residues
MSKERILVVEDQPDISSLLKIYFTAQGYEVYTALRGQQALEICSKTPPNLALLDVNLPDMEGYDVGRALRASPRTRHIPI
IFLTARGERRDRLIGLGEVQAQYYIVKPFDIEEVHTIVKNQLEEARRRNQLHPVTNLPTAELLNDQLRQLLVAPRWALIN
VHINGFETFTNVYGAVVGEDVLRFVALMLNEVVSELGGNEEFVGQQSVGQSFMITTLPERSHAICERIIARFDEEIGLHY
NYRHRKQGYIEFVDDTGETRQAPLMSLSIGVLTQDDGPFADIRELSEVAEEVRQRALIQAREQGKRSFAIYGRS
>Mature_313_residues
SKERILVVEDQPDISSLLKIYFTAQGYEVYTALRGQQALEICSKTPPNLALLDVNLPDMEGYDVGRALRASPRTRHIPII
FLTARGERRDRLIGLGEVQAQYYIVKPFDIEEVHTIVKNQLEEARRRNQLHPVTNLPTAELLNDQLRQLLVAPRWALINV
HINGFETFTNVYGAVVGEDVLRFVALMLNEVVSELGGNEEFVGQQSVGQSFMITTLPERSHAICERIIARFDEEIGLHYN
YRHRKQGYIEFVDDTGETRQAPLMSLSIGVLTQDDGPFADIRELSEVAEEVRQRALIQAREQGKRSFAIYGRS

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=128, Percent_Identity=39.84375, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790860, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787229, Length=130, Percent_Identity=38.4615384615385, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790863, Length=122, Percent_Identity=36.0655737704918, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=33.6134453781513, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789809, Length=137, Percent_Identity=33.5766423357664, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=115, Percent_Identity=37.3913043478261, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=33.8983050847458, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082012, Length=133, Percent_Identity=30.8270676691729, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI2367329, Length=110, Percent_Identity=40.9090909090909, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 35708; Mature: 35577

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKERILVVEDQPDISSLLKIYFTAQGYEVYTALRGQQALEICSKTPPNLALLDVNLPDM
CCCCCEEEEECCCCHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHCCCCCEEEEECCCCCC
EGYDVGRALRASPRTRHIPIIFLTARGERRDRLIGLGEVQAQYYIVKPFDIEEVHTIVKN
CCCCCCHHHHCCCCCCCCEEEEEECCCCCCHHEEECEEEEEEEEEECCCCHHHHHHHHHH
QLEEARRRNQLHPVTNLPTAELLNDQLRQLLVAPRWALINVHINGFETFTNVYGAVVGED
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHH
VLRFVALMLNEVVSELGGNEEFVGQQSVGQSFMITTLPERSHAICERIIARFDEEIGLHY
HHHHHHHHHHHHHHHHCCCHHHCCHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEE
NYRHRKQGYIEFVDDTGETRQAPLMSLSIGVLTQDDGPFADIRELSEVAEEVRQRALIQA
CEECCCCCCEEEECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
REQGKRSFAIYGRS
HHCCCCEEEEEECC
>Mature Secondary Structure 
SKERILVVEDQPDISSLLKIYFTAQGYEVYTALRGQQALEICSKTPPNLALLDVNLPDM
CCCCEEEEECCCCHHHHHHHHHCCCCCEEEHHHCCHHHHHHHHCCCCCEEEEECCCCCC
EGYDVGRALRASPRTRHIPIIFLTARGERRDRLIGLGEVQAQYYIVKPFDIEEVHTIVKN
CCCCCCHHHHCCCCCCCCEEEEEECCCCCCHHEEECEEEEEEEEEECCCCHHHHHHHHHH
QLEEARRRNQLHPVTNLPTAELLNDQLRQLLVAPRWALINVHINGFETFTNVYGAVVGED
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHHH
VLRFVALMLNEVVSELGGNEEFVGQQSVGQSFMITTLPERSHAICERIIARFDEEIGLHY
HHHHHHHHHHHHHHHHCCCHHHCCHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCEE
NYRHRKQGYIEFVDDTGETRQAPLMSLSIGVLTQDDGPFADIRELSEVAEEVRQRALIQA
CEECCCCCCEEEECCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
REQGKRSFAIYGRS
HHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]