Click here to switch to the map view.

The map label for this gene is rho [H]

Identifier: 222525941

GI number: 222525941

Start: 3329088

End: 3330350

Strand: Direct

Name: rho [H]

Synonym: Chy400_2697

Alternate gene names: 222525941

Gene position: 3329088-3330350 (Clockwise)

Preceding gene: 222525940

Following gene: 222525942

Centisome position: 63.18

GC content: 57.64

Gene sequence:

>1263_bases
ATGGAAGAAAAACGTATGACAACAGGAACCAGTGAAACTACAGAGCGAACTCGTCGCCGTCGCCGTCGGGTGAATGGAGA
TGGCGGAGCACCTGCTGATGGTGAGGCTTTGTCCGCACCGGTTGTAACGACCACAGTTGTTGAAGAACCGGTCACAACGC
AGGGTGCTGGTTTACTGGAAATTGTGCCTGATGGCCATGGCTTCCTGCGCAATGCGCGCCTCAGCCCCAGCAACGATGAC
GTCTATGTTGCTCAGTCGCAGATTCGTCGCTTCAATCTGCGTACCGGTGATATGATTGAAGGTCGGGTGCGCCCACCCAA
AGAGGTTGAGCGTTATCCATCACTGCTCTATGTCGAACGGATCAACGGTCTGCCGGCTGAAATGGCGCAGAAGCGCCCCC
TCTTCGAGCAGTTAACCCCCATTCATCCGAATGTGCAGATTGTCCTCTCGACCGAGCCAAATATTCTGCCAACCCGCATT
GTTGACGTGATCGCTCCGATTGGGCGTGGGCAGCGCGGGTTGATTGTCGCTCCCCCCAAAGCCGGCAAGACGATGCTGCT
GAAGGCGATTGCCAATGGTGTGACGACCAACGCGCCCGATATTCAGCTCATTGTGTTGTTGATCGGCGAGCGCCCGGAGG
AAGTTACCGACATGCGACGGTCGGTGAAGGGTGAGGTGGTGGCGGCTACCTTCGATGAGCCGGTCGAGCAGCATATCAAG
GTCGCGGAACTGGTATTGGAAAAGGCCAAGCGTCAGGTTGAGCACGGGCGCCATGTGGTGATCCTGATGGATTCGCTGAC
TCGTCTTACCCGTGCCTATAACATCGCCATGCCACCGAGTGGGCGCACCCTCTCCGGTGGTGTTGATCCGGCAGCCCTCT
ACCCGCCCAAACGCTTTTTCGGCTCGGCGCGGAATATCGAGGATGGCGGTTCACTGACCATCATTGCCACCTGTTTGGTA
GATACCGGTTCGCGCATGGACGATGTTATCTACGAAGAGTTCAAGGGCACCGGGAACATGGAACTGCACCTCGACCGCAA
ACTGGCCGAGAAGCGCATCTTCCCCGCCGTGGATATCCAGCGTTCGGGAACCCGCCGCGAAGACCTCTTGCTCGATCCGG
TGACCCTGCGCCAGAGCTGGATGTTGCGCCGGATGGTTAGCATGGTCGGCGAAAACGAGGGTGCAGAGCTGATGCTGACC
CGTATGGCCAAGACGAAGAGCAATGCCGAGTTTCTCGCCTCACTCGGTAAGGTTGGCTCGTAA

Upstream 100 bases:

>100_bases
GCTATCGTTCTGGCTCATCGAGCCGCTTACCGGCAGCTCCATCCGGTGAAACAGCGCATCCCGGCGAATCATCTCACAAA
GAGGAGTAAAAAACTACACA

Downstream 100 bases:

>100_bases
ATACCAACTGCTCCTATCAATAATGTCTTGTTATGTCCAGGCATCCGTTAAGCGGTATTGTGATTGCTGGTGGACGTAGT
CGGCGTTTTGGTGCCGATAA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MEEKRMTTGTSETTERTRRRRRRVNGDGGAPADGEALSAPVVTTTVVEEPVTTQGAGLLEIVPDGHGFLRNARLSPSNDD
VYVAQSQIRRFNLRTGDMIEGRVRPPKEVERYPSLLYVERINGLPAEMAQKRPLFEQLTPIHPNVQIVLSTEPNILPTRI
VDVIAPIGRGQRGLIVAPPKAGKTMLLKAIANGVTTNAPDIQLIVLLIGERPEEVTDMRRSVKGEVVAATFDEPVEQHIK
VAELVLEKAKRQVEHGRHVVILMDSLTRLTRAYNIAMPPSGRTLSGGVDPAALYPPKRFFGSARNIEDGGSLTIIATCLV
DTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAVDIQRSGTRREDLLLDPVTLRQSWMLRRMVSMVGENEGAELMLT
RMAKTKSNAEFLASLGKVGS

Sequences:

>Translated_420_residues
MEEKRMTTGTSETTERTRRRRRRVNGDGGAPADGEALSAPVVTTTVVEEPVTTQGAGLLEIVPDGHGFLRNARLSPSNDD
VYVAQSQIRRFNLRTGDMIEGRVRPPKEVERYPSLLYVERINGLPAEMAQKRPLFEQLTPIHPNVQIVLSTEPNILPTRI
VDVIAPIGRGQRGLIVAPPKAGKTMLLKAIANGVTTNAPDIQLIVLLIGERPEEVTDMRRSVKGEVVAATFDEPVEQHIK
VAELVLEKAKRQVEHGRHVVILMDSLTRLTRAYNIAMPPSGRTLSGGVDPAALYPPKRFFGSARNIEDGGSLTIIATCLV
DTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAVDIQRSGTRREDLLLDPVTLRQSWMLRRMVSMVGENEGAELMLT
RMAKTKSNAEFLASLGKVGS
>Mature_420_residues
MEEKRMTTGTSETTERTRRRRRRVNGDGGAPADGEALSAPVVTTTVVEEPVTTQGAGLLEIVPDGHGFLRNARLSPSNDD
VYVAQSQIRRFNLRTGDMIEGRVRPPKEVERYPSLLYVERINGLPAEMAQKRPLFEQLTPIHPNVQIVLSTEPNILPTRI
VDVIAPIGRGQRGLIVAPPKAGKTMLLKAIANGVTTNAPDIQLIVLLIGERPEEVTDMRRSVKGEVVAATFDEPVEQHIK
VAELVLEKAKRQVEHGRHVVILMDSLTRLTRAYNIAMPPSGRTLSGGVDPAALYPPKRFFGSARNIEDGGSLTIIATCLV
DTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAVDIQRSGTRREDLLLDPVTLRQSWMLRRMVSMVGENEGAELMLT
RMAKTKSNAEFLASLGKVGS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=365, Percent_Identity=60, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46363; Mature: 46363

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKRMTTGTSETTERTRRRRRRVNGDGGAPADGEALSAPVVTTTVVEEPVTTQGAGLLE
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEE
IVPDGHGFLRNARLSPSNDDVYVAQSQIRRFNLRTGDMIEGRVRPPKEVERYPSLLYVER
ECCCCCCHHCCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEEHH
INGLPAEMAQKRPLFEQLTPIHPNVQIVLSTEPNILPTRIVDVIAPIGRGQRGLIVAPPK
HCCCCHHHHHCCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCC
AGKTMLLKAIANGVTTNAPDIQLIVLLIGERPEEVTDMRRSVKGEVVAATFDEPVEQHIK
CCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHHH
VAELVLEKAKRQVEHGRHVVILMDSLTRLTRAYNIAMPPSGRTLSGGVDPAALYPPKRFF
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEECCCCCCCCCCCCCCHHCCCCHHHH
GSARNIEDGGSLTIIATCLVDTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAVDIQ
CCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCC
RSGTRREDLLLDPVTLRQSWMLRRMVSMVGENEGAELMLTRMAKTKSNAEFLASLGKVGS
CCCCCCHHEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MEEKRMTTGTSETTERTRRRRRRVNGDGGAPADGEALSAPVVTTTVVEEPVTTQGAGLLE
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEE
IVPDGHGFLRNARLSPSNDDVYVAQSQIRRFNLRTGDMIEGRVRPPKEVERYPSLLYVER
ECCCCCCHHCCCCCCCCCCCEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEEHH
INGLPAEMAQKRPLFEQLTPIHPNVQIVLSTEPNILPTRIVDVIAPIGRGQRGLIVAPPK
HCCCCHHHHHCCCHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEECCC
AGKTMLLKAIANGVTTNAPDIQLIVLLIGERPEEVTDMRRSVKGEVVAATFDEPVEQHIK
CCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCEEEEECCHHHHHHHH
VAELVLEKAKRQVEHGRHVVILMDSLTRLTRAYNIAMPPSGRTLSGGVDPAALYPPKRFF
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHEEECCCCCCCCCCCCCCHHCCCCHHHH
GSARNIEDGGSLTIIATCLVDTGSRMDDVIYEEFKGTGNMELHLDRKLAEKRIFPAVDIQ
CCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECHHHHHHHCCCCCCCC
RSGTRREDLLLDPVTLRQSWMLRRMVSMVGENEGAELMLTRMAKTKSNAEFLASLGKVGS
CCCCCCHHEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423140; 9353933; 9384377 [H]