Click here to switch to the map view.

The map label for this gene is mhpD3 [H]

Identifier: 222524752

GI number: 222524752

Start: 1873965

End: 1874786

Strand: Direct

Name: mhpD3 [H]

Synonym: Chy400_1479

Alternate gene names: 222524752

Gene position: 1873965-1874786 (Clockwise)

Preceding gene: 222524751

Following gene: 222524753

Centisome position: 35.57

GC content: 56.93

Gene sequence:

>822_bases
ATGCCTGAGCAATCAGTAACAACCCAAACCACCGATGACGCACTTATTATCGCTCTGGCACAACGGCTCGATCAGGCCTG
GGAAGAGCGCTTCACCATACTTCCCCTCACCGAAAGCGAGGGCATTACCAATGCAGACATTGCTTACCGGATTCAGACGC
ATTGGACAGACATGCGCGTTCAGCGCGGCGAGCGGATCATTGGGCGCAAAATCGGCCTGACCAGCAAAGCTGTGCAAGAG
CAGATGGGCGTCAACGAGCCGGATTATGGCACTCTATGGGCTTCGCGCTATTTTCCGGCCAGAAATGGGCGCGCTGAAGC
GCCTTTTGAAATCTTTCTCCAACCGCGGGTCGAGGGTGAAATTGCGTTTCTAATTGGAGAAACCCTCGACTGGCCGGATA
TTACCCCGCAACAGGTGCTGGCAGCAACCGAGGCACTGGCGGCAGCTATCGAAATTGTGGATAGTCGCATTGATCGCTGG
CGCATTAAGCTAGCCGACACGATTGCCGATAACGCCTCCTACGGTGGCTTGACGATTGGCGCCTGGAGCAGGGCAATGCG
CAGCAGCGACCTGCGCACCATTGGTATGGTGATGAGCCACAATGGTCGCCCCACCGTCTTTGGTACCGGTGCCGCTGCGC
TTGGGCATCCGGCACTGGCGGTGGCATGGCTGGCGAACAAACTGCGCGCATTCGGGATTCCGCTGCGTGCCGGTGATATT
GTGATCTCTGGAGCTTTGGCAGCAACAATTCCGGTGCAACGCGGCGATCTGTTCACGCTGGAAATGCATGATCAACCACC
ACTCCACTTACGGTTCGTGTGA

Upstream 100 bases:

>100_bases
TGCGCGATCTGCGCGTACCTTTCGGTGGTATGAAACAGAGTGGAATTGGCCGGGAAGGTGGGCACTACAGCCTTGAGTTC
TACACGGAGGCAAAGACGAT

Downstream 100 bases:

>100_bases
AGGAGATTCAGATGCCGATGCTCGAAGTCTTCTACAGCGGGGATCGACCGCCAGACCGTACCCGGAAGCAGGCATTTGCT
GCTGAAGCCAGTGCTATCTT

Product: 4-oxalocrotonate decarboxylase

Products: NA

Alternate protein names: 2-hydroxypentadienoic acid hydratase 3 [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MPEQSVTTQTTDDALIIALAQRLDQAWEERFTILPLTESEGITNADIAYRIQTHWTDMRVQRGERIIGRKIGLTSKAVQE
QMGVNEPDYGTLWASRYFPARNGRAEAPFEIFLQPRVEGEIAFLIGETLDWPDITPQQVLAATEALAAAIEIVDSRIDRW
RIKLADTIADNASYGGLTIGAWSRAMRSSDLRTIGMVMSHNGRPTVFGTGAAALGHPALAVAWLANKLRAFGIPLRAGDI
VISGALAATIPVQRGDLFTLEMHDQPPLHLRFV

Sequences:

>Translated_273_residues
MPEQSVTTQTTDDALIIALAQRLDQAWEERFTILPLTESEGITNADIAYRIQTHWTDMRVQRGERIIGRKIGLTSKAVQE
QMGVNEPDYGTLWASRYFPARNGRAEAPFEIFLQPRVEGEIAFLIGETLDWPDITPQQVLAATEALAAAIEIVDSRIDRW
RIKLADTIADNASYGGLTIGAWSRAMRSSDLRTIGMVMSHNGRPTVFGTGAAALGHPALAVAWLANKLRAFGIPLRAGDI
VISGALAATIPVQRGDLFTLEMHDQPPLHLRFV
>Mature_272_residues
PEQSVTTQTTDDALIIALAQRLDQAWEERFTILPLTESEGITNADIAYRIQTHWTDMRVQRGERIIGRKIGLTSKAVQEQ
MGVNEPDYGTLWASRYFPARNGRAEAPFEIFLQPRVEGEIAFLIGETLDWPDITPQQVLAATEALAAAIEIVDSRIDRWR
IKLADTIADNASYGGLTIGAWSRAMRSSDLRTIGMVMSHNGRPTVFGTGAAALGHPALAVAWLANKLRAFGIPLRAGDIV
ISGALAATIPVQRGDLFTLEMHDQPPLHLRFV

Specific function: Catalyzes the conversion of 2-hydroxypentadienoic acid (enolic form of 2-oxopent-4-enoate) to 4-hydroxy-2-ketopentanoic acid [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family. MhpD subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=241, Percent_Identity=42.3236514522822, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =4.2.1.80 [H]

Molecular weight: Translated: 30070; Mature: 29939

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEQSVTTQTTDDALIIALAQRLDQAWEERFTILPLTESEGITNADIAYRIQTHWTDMRV
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEECCHHHHH
QRGERIIGRKIGLTSKAVQEQMGVNEPDYGTLWASRYFPARNGRAEAPFEIFLQPRVEGE
HCCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEHHHCCCCCCCCCCCCEEEEEECCCCCE
IAFLIGETLDWPDITPQQVLAATEALAAAIEIVDSRIDRWRIKLADTIADNASYGGLTIG
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHCCCCCCCEEEH
AWSRAMRSSDLRTIGMVMSHNGRPTVFGTGAAALGHPALAVAWLANKLRAFGIPLRAGDI
HHHHHHHHCCCCEEEHEEECCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHCCCEECCCE
VISGALAATIPVQRGDLFTLEMHDQPPLHLRFV
EEECCEEEEEEECCCCEEEEEECCCCCEEEEEC
>Mature Secondary Structure 
PEQSVTTQTTDDALIIALAQRLDQAWEERFTILPLTESEGITNADIAYRIQTHWTDMRV
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEECCHHHHH
QRGERIIGRKIGLTSKAVQEQMGVNEPDYGTLWASRYFPARNGRAEAPFEIFLQPRVEGE
HCCCHHHHHHCCCCHHHHHHHHCCCCCCCCEEEHHHCCCCCCCCCCCCEEEEEECCCCCE
IAFLIGETLDWPDITPQQVLAATEALAAAIEIVDSRIDRWRIKLADTIADNASYGGLTIG
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHHHHCCCCCCCEEEH
AWSRAMRSSDLRTIGMVMSHNGRPTVFGTGAAALGHPALAVAWLANKLRAFGIPLRAGDI
HHHHHHHHCCCCEEEHEEECCCCCEEEECCCHHHCCHHHHHHHHHHHHHHHCCCEECCCE
VISGALAATIPVQRGDLFTLEMHDQPPLHLRFV
EEECCEEEEEEECCCCEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9791097; 12823813 [H]