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The map label for this gene is dmpD [H]

Identifier: 222524748

GI number: 222524748

Start: 1870664

End: 1871503

Strand: Direct

Name: dmpD [H]

Synonym: Chy400_1475

Alternate gene names: 222524748

Gene position: 1870664-1871503 (Clockwise)

Preceding gene: 222524747

Following gene: 222524749

Centisome position: 35.5

GC content: 57.02

Gene sequence:

>840_bases
ATGGTTGAACACATCATCACCGGTCAATTCACAACCGCCCTCCATCGACAGGGGTCTGGCGATGCACCGGCCATCCTCTT
CATTCACGGCTCCGGCCCAGGTGCCAATGGCTGGTCGAACTGGAACCTGGCGCTACCGGCACTGGGTGAACAGTGGTACT
GTCTGGCGCCTGACCTGATCGGATTTGGCGATAGTCAGCACCCCGATCCACCACCGACAGACATTCGGCGCTGGATGCGG
TTGTGGGTTGATCAGATGCTGGCTCTGCTCGATACGTTGGGATTAGCCAGGGTTGATCTGGTTGGCAATTCGATGGGTGG
GGCAGTTGCGTTGCACTTGTTACTGGAAGCGCCAGAACGATTTCGGCGGGTGGTACTGATGGGAGCGATTGGCGCACCCT
GTCGGCTGACACCAGAACTTGATCGCCTGTGGGGGTTTTACGACGATCCATCGGCAGCCTTGCTGGCCCAGGCCATTCGC
TGGTTTGTCTACGATGATGCGTTTGTGCGCGACCGGCTTGATGAGATTGTGCGGGTACGCTATACCGCAGCGATGCGTAC
TGACGTTTGGCGCTCGTATACTGCCATGTTTCCGGCTCCCCGTCAGCAACACCTCGATGCATTGATCGTACCCGATGCCG
CGTTACGCCGGATGACCCATCCAGTGCTGCTGATCCACGGACGCGATGATGCCATTGTCCCGGCTGATACCAGCTACTAT
TTGCTCAACCGGCTTCCCAATGCTGAACTCCATGTGATAGGACGCTGTAGCCACTGGACCCAGATTGAACACAGTCAACG
CTTTCATCAGCTTGTGCGTACATTTTTTGCCGAGACCTGA

Upstream 100 bases:

>100_bases
AGATGGCTGCCGTGTAGATGCGAGAGGATACACGATCCTTAATCATCTACATTCCAAGACCTGGTAGACAGCTACAGTCT
GTACAAGACAGGATAAATCG

Downstream 100 bases:

>100_bases
GAGGACGGTGATATGCTACTTCTGCGCATTACGATGCTGGAGGGGAGGAGCACAGAGCAAAAGGCTGAACTGGCCCGTGC
GCTTTCGGCAGCAGCGGCAG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MVEHIITGQFTTALHRQGSGDAPAILFIHGSGPGANGWSNWNLALPALGEQWYCLAPDLIGFGDSQHPDPPPTDIRRWMR
LWVDQMLALLDTLGLARVDLVGNSMGGAVALHLLLEAPERFRRVVLMGAIGAPCRLTPELDRLWGFYDDPSAALLAQAIR
WFVYDDAFVRDRLDEIVRVRYTAAMRTDVWRSYTAMFPAPRQQHLDALIVPDAALRRMTHPVLLIHGRDDAIVPADTSYY
LLNRLPNAELHVIGRCSHWTQIEHSQRFHQLVRTFFAET

Sequences:

>Translated_279_residues
MVEHIITGQFTTALHRQGSGDAPAILFIHGSGPGANGWSNWNLALPALGEQWYCLAPDLIGFGDSQHPDPPPTDIRRWMR
LWVDQMLALLDTLGLARVDLVGNSMGGAVALHLLLEAPERFRRVVLMGAIGAPCRLTPELDRLWGFYDDPSAALLAQAIR
WFVYDDAFVRDRLDEIVRVRYTAAMRTDVWRSYTAMFPAPRQQHLDALIVPDAALRRMTHPVLLIHGRDDAIVPADTSYY
LLNRLPNAELHVIGRCSHWTQIEHSQRFHQLVRTFFAET
>Mature_279_residues
MVEHIITGQFTTALHRQGSGDAPAILFIHGSGPGANGWSNWNLALPALGEQWYCLAPDLIGFGDSQHPDPPPTDIRRWMR
LWVDQMLALLDTLGLARVDLVGNSMGGAVALHLLLEAPERFRRVVLMGAIGAPCRLTPELDRLWGFYDDPSAALLAQAIR
WFVYDDAFVRDRLDEIVRVRYTAAMRTDVWRSYTAMFPAPRQQHLDALIVPDAALRRMTHPVLLIHGRDDAIVPADTSYY
LLNRLPNAELHVIGRCSHWTQIEHSQRFHQLVRTFFAET

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=268, Percent_Identity=31.7164179104478, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31519; Mature: 31519

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEHIITGQFTTALHRQGSGDAPAILFIHGSGPGANGWSNWNLALPALGEQWYCLAPDLI
CCCCEECCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCCCCEEEECCHHH
GFGDSQHPDPPPTDIRRWMRLWVDQMLALLDTLGLARVDLVGNSMGGAVALHLLLEAPER
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCCCCHHHHHHHHHHCHHH
FRRVVLMGAIGAPCRLTPELDRLWGFYDDPSAALLAQAIRWFVYDDAFVRDRLDEIVRVR
HHHHHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YTAAMRTDVWRSYTAMFPAPRQQHLDALIVPDAALRRMTHPVLLIHGRDDAIVPADTSYY
HHHHHHHHHHHHHHHCCCCCHHHCCCEEECCHHHHHHCCCCEEEEECCCCCEECCCCHHH
LLNRLPNAELHVIGRCSHWTQIEHSQRFHQLVRTFFAET
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEHIITGQFTTALHRQGSGDAPAILFIHGSGPGANGWSNWNLALPALGEQWYCLAPDLI
CCCCEECCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCEECCCCCCCEEEECCHHH
GFGDSQHPDPPPTDIRRWMRLWVDQMLALLDTLGLARVDLVGNSMGGAVALHLLLEAPER
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHEEECCCCCCHHHHHHHHHHCHHH
FRRVVLMGAIGAPCRLTPELDRLWGFYDDPSAALLAQAIRWFVYDDAFVRDRLDEIVRVR
HHHHHHHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YTAAMRTDVWRSYTAMFPAPRQQHLDALIVPDAALRRMTHPVLLIHGRDDAIVPADTSYY
HHHHHHHHHHHHHHHCCCCCHHHCCCEEECCHHHHHHCCCCEEEEECCCCCEECCCCHHH
LLNRLPNAELHVIGRCSHWTQIEHSQRFHQLVRTFFAET
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194577 [H]