Click here to switch to the map view.

The map label for this gene is ptsI [H]

Identifier: 222524717

GI number: 222524717

Start: 1831332

End: 1832990

Strand: Direct

Name: ptsI [H]

Synonym: Chy400_1444

Alternate gene names: 222524717

Gene position: 1831332-1832990 (Clockwise)

Preceding gene: 222524716

Following gene: 222524718

Centisome position: 34.76

GC content: 62.81

Gene sequence:

>1659_bases
ATGTCGGCGACACTTCGTGGTGCAGGGGGAGCTGCCGGTTTGGCGTTAGGGCCAGCGTACCACTGGCAACGAGTGTCCAT
CCCTACCGATCCACCCAATGAACCGCCAGAAGCAGCCCTGGCCCGTTTTCACGCTGCCCAACACACCGCGGCCCACCGCC
TACGGGCACTGGCTGAACGGCAGCGTGCTGCCGGCCTGCGCGAAGCCGATCTCTTCGATGCACAGGCGCTGCTGGTTGAA
GATGAAACGCTGACTGACGGCGTAACTGCATTGCTGCTCGACGGTCAGCCCCTCATTCCTGCGATTCAGACGGTGGTGCG
CCAGATGGAAGAGGCGCTGGCCGCGCTTGACGATGACTATTTACGTGAACGGGCAGCCGATATTGCCGCGGTTGGAGTTG
AGTTGCTGCGTGCGCTGAGCGGAGATCACGCGCCACCGCCGATCCCACCCGGCGCCATTGTTATCGCCGATGATCTGACT
CCCGCAGAGACGGTTGATCTTCCGCATCACGTTGCCGGCTTTGCCACTGCCGGCGGTGGCCCAACCGGTCATACCATCAT
CCTGGCCCGTTCGCGTGGGGTGCCGGCGGTTGTCGGGTTGGGAGAGGCTATTCTGCACATCCCCGCCGGTACGACGCTTC
TTCTCGATGGTGATGCGGCTTTGGTGATGGTTGATCCAGATGAAGCGGCACTACAGGCAGCTCAGGAACGAATGACCGCC
ATTCAGGAGACCCGCCGCCGCCAGGCGGCCCTGCGCGATCTTCCCGGTCGGTTGCGCGATGGCCGCTCAATCGCGCTGTG
GGCAAATATCGGTCGTCCGGCCGAGGCGCGCCTGGCCCGTGACAACGGTGCCGAAGGGATTGGCCTCTTTCGGACCGAGT
TTCTCTTCCTTGATCGCAGTGCACCTCCCGATGAAGACGAGCAATTTGCAGCGTATCGCGCCGTCATCACCGAACTGCCC
AACCGTCCGATTGTGATCCGGACGCTCGATGTAGGTGGCGATAAGCCGCTGCCCTACCTACCGCTTCCCCCTGAAGCCAA
TCCATTTCTGGGGGTGCGTGGCGTTCGGCTGTCGATGCAGCGTCCTGATCTCTTCCAGGTGCAATTACGGGCATTGCTGC
GTGCTGCCTTCTACGGCGACATCTGGATTATGCTGCCGATGGTCACCACGCTGGCCGACCTGGTCTGGGGGCAAGAGCAG
ATGCGTGAAGCAGCCGCCGCGCTGGCCGCAGCCGGGATTGAACATCGCGCCGATCCGCCGCTGGGCATTATGATTGAGAC
ACCGGCAGCGGCGGTACTGGCCGATCAGTTTGCACGCCACGCCGCTTTCTTCAGTATTGGCAGTAACGATCTTGCCCAGT
ACACGCTGGCGGTTGATCGTGGTCACCCGACGCTGGCCACGCAATATCGGGCCGATGATCCGGCAGTGTGGCGGATGATT
GATTTGGCCGCTCGCGCCGCACAGCAGGCCGGTATTCCGATTGGCATCTGTGGCGAACTCGGTGGCGAACCGGATGCTGC
GGTTGCCCTGGCCGGCCTGGGCCTCAACGAATTGAGCATGGCCCCGGCACGTATCCCGGTCGTCAAAGAGCGATTGGCGC
AGACTTCGTGGGCTGAAGCGCAGGCCGCTGCAGCCCGGATGGTGCAGCGGGTTGAATGA

Upstream 100 bases:

>100_bases
TCCGGTTGCGTGCCAGTGGTGAAGATGAGGCAGAAGTCATTGCTGCTCTAACCAAACTGGTGGAAGAGAATTTTGGCGAG
TAGCAGGTAGAGGCATGATG

Downstream 100 bases:

>100_bases
GTCTGAGAGGTTCTATGTCCGAGCGACCCTGGATCGAACTCTTCCCCGGCGTTCACCGCCGGCGCATTGCCCTCACCGAC
CGTATCTATCAGATGGAGGT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MSATLRGAGGAAGLALGPAYHWQRVSIPTDPPNEPPEAALARFHAAQHTAAHRLRALAERQRAAGLREADLFDAQALLVE
DETLTDGVTALLLDGQPLIPAIQTVVRQMEEALAALDDDYLRERAADIAAVGVELLRALSGDHAPPPIPPGAIVIADDLT
PAETVDLPHHVAGFATAGGGPTGHTIILARSRGVPAVVGLGEAILHIPAGTTLLLDGDAALVMVDPDEAALQAAQERMTA
IQETRRRQAALRDLPGRLRDGRSIALWANIGRPAEARLARDNGAEGIGLFRTEFLFLDRSAPPDEDEQFAAYRAVITELP
NRPIVIRTLDVGGDKPLPYLPLPPEANPFLGVRGVRLSMQRPDLFQVQLRALLRAAFYGDIWIMLPMVTTLADLVWGQEQ
MREAAAALAAAGIEHRADPPLGIMIETPAAAVLADQFARHAAFFSIGSNDLAQYTLAVDRGHPTLATQYRADDPAVWRMI
DLAARAAQQAGIPIGICGELGGEPDAAVALAGLGLNELSMAPARIPVVKERLAQTSWAEAQAAAARMVQRVE

Sequences:

>Translated_552_residues
MSATLRGAGGAAGLALGPAYHWQRVSIPTDPPNEPPEAALARFHAAQHTAAHRLRALAERQRAAGLREADLFDAQALLVE
DETLTDGVTALLLDGQPLIPAIQTVVRQMEEALAALDDDYLRERAADIAAVGVELLRALSGDHAPPPIPPGAIVIADDLT
PAETVDLPHHVAGFATAGGGPTGHTIILARSRGVPAVVGLGEAILHIPAGTTLLLDGDAALVMVDPDEAALQAAQERMTA
IQETRRRQAALRDLPGRLRDGRSIALWANIGRPAEARLARDNGAEGIGLFRTEFLFLDRSAPPDEDEQFAAYRAVITELP
NRPIVIRTLDVGGDKPLPYLPLPPEANPFLGVRGVRLSMQRPDLFQVQLRALLRAAFYGDIWIMLPMVTTLADLVWGQEQ
MREAAAALAAAGIEHRADPPLGIMIETPAAAVLADQFARHAAFFSIGSNDLAQYTLAVDRGHPTLATQYRADDPAVWRMI
DLAARAAQQAGIPIGICGELGGEPDAAVALAGLGLNELSMAPARIPVVKERLAQTSWAEAQAAAARMVQRVE
>Mature_551_residues
SATLRGAGGAAGLALGPAYHWQRVSIPTDPPNEPPEAALARFHAAQHTAAHRLRALAERQRAAGLREADLFDAQALLVED
ETLTDGVTALLLDGQPLIPAIQTVVRQMEEALAALDDDYLRERAADIAAVGVELLRALSGDHAPPPIPPGAIVIADDLTP
AETVDLPHHVAGFATAGGGPTGHTIILARSRGVPAVVGLGEAILHIPAGTTLLLDGDAALVMVDPDEAALQAAQERMTAI
QETRRRQAALRDLPGRLRDGRSIALWANIGRPAEARLARDNGAEGIGLFRTEFLFLDRSAPPDEDEQFAAYRAVITELPN
RPIVIRTLDVGGDKPLPYLPLPPEANPFLGVRGVRLSMQRPDLFQVQLRALLRAAFYGDIWIMLPMVTTLADLVWGQEQM
REAAAALAAAGIEHRADPPLGIMIETPAAAVLADQFARHAAFFSIGSNDLAQYTLAVDRGHPTLATQYRADDPAVWRMID
LAARAAQQAGIPIGICGELGGEPDAAVALAGLGLNELSMAPARIPVVKERLAQTSWAEAQAAAARMVQRVE

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=544, Percent_Identity=37.3161764705882, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI48994992, Length=491, Percent_Identity=37.8818737270876, Blast_Score=319, Evalue=4e-88,
Organism=Escherichia coli, GI1788726, Length=517, Percent_Identity=38.6847195357834, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1789193, Length=556, Percent_Identity=33.4532374100719, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1787994, Length=427, Percent_Identity=27.1662763466042, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 58668; Mature: 58537

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLRGAGGAAGLALGPAYHWQRVSIPTDPPNEPPEAALARFHAAQHTAAHRLRALAER
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRAAGLREADLFDAQALLVEDETLTDGVTALLLDGQPLIPAIQTVVRQMEEALAALDDDY
HHHCCCCHHHHCCCEEEEEECCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHH
LRERAADIAAVGVELLRALSGDHAPPPIPPGAIVIADDLTPAETVDLPHHVAGFATAGGG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHCCCCHHHHCEEECCCC
PTGHTIILARSRGVPAVVGLGEAILHIPAGTTLLLDGDAALVMVDPDEAALQAAQERMTA
CCCCEEEEEECCCCCEEEECCCEEEECCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHH
IQETRRRQAALRDLPGRLRDGRSIALWANIGRPAEARLARDNGAEGIGLFRTEFLFLDRS
HHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCEEEHEEEEEEEECC
APPDEDEQFAAYRAVITELPNRPIVIRTLDVGGDKPLPYLPLPPEANPFLGVRGVRLSMQ
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECC
RPDLFQVQLRALLRAAFYGDIWIMLPMVTTLADLVWGQEQMREAAAALAAAGIEHRADPP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
LGIMIETPAAAVLADQFARHAAFFSIGSNDLAQYTLAVDRGHPTLATQYRADDPAVWRMI
EEEEECCCHHHHHHHHHHHHHHEEECCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHH
DLAARAAQQAGIPIGICGELGGEPDAAVALAGLGLNELSMAPARIPVVKERLAQTSWAEA
HHHHHHHHHCCCCEEEECCCCCCCCHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHH
QAAAARMVQRVE
HHHHHHHHHHCC
>Mature Secondary Structure 
SATLRGAGGAAGLALGPAYHWQRVSIPTDPPNEPPEAALARFHAAQHTAAHRLRALAER
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRAAGLREADLFDAQALLVEDETLTDGVTALLLDGQPLIPAIQTVVRQMEEALAALDDDY
HHHCCCCHHHHCCCEEEEEECCCHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHH
LRERAADIAAVGVELLRALSGDHAPPPIPPGAIVIADDLTPAETVDLPHHVAGFATAGGG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHCCCCHHHHCEEECCCC
PTGHTIILARSRGVPAVVGLGEAILHIPAGTTLLLDGDAALVMVDPDEAALQAAQERMTA
CCCCEEEEEECCCCCEEEECCCEEEECCCCCEEEEECCEEEEEECCCHHHHHHHHHHHHH
IQETRRRQAALRDLPGRLRDGRSIALWANIGRPAEARLARDNGAEGIGLFRTEFLFLDRS
HHHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHHHHHCCCCCCCEEEHEEEEEEEECC
APPDEDEQFAAYRAVITELPNRPIVIRTLDVGGDKPLPYLPLPPEANPFLGVRGVRLSMQ
CCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECC
RPDLFQVQLRALLRAAFYGDIWIMLPMVTTLADLVWGQEQMREAAAALAAAGIEHRADPP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCC
LGIMIETPAAAVLADQFARHAAFFSIGSNDLAQYTLAVDRGHPTLATQYRADDPAVWRMI
EEEEECCCHHHHHHHHHHHHHHEEECCCCCCEEEEEEEECCCCCEEEECCCCCHHHHHHH
DLAARAAQQAGIPIGICGELGGEPDAAVALAGLGLNELSMAPARIPVVKERLAQTSWAEA
HHHHHHHHHCCCCEEEECCCCCCCCHHHEEECCCCHHHCCCCCCCHHHHHHHHHHHHHHH
QAAAARMVQRVE
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]