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The map label for this gene is bglIIM [H]

Identifier: 222524637

GI number: 222524637

Start: 1741454

End: 1742443

Strand: Direct

Name: bglIIM [H]

Synonym: Chy400_1361

Alternate gene names: 222524637

Gene position: 1741454-1742443 (Clockwise)

Preceding gene: 222524635

Following gene: 222524638

Centisome position: 33.05

GC content: 49.49

Gene sequence:

>990_bases
ATGAGTGAATTACCATTGTTTCGTTCGGTAACGTCTATCGAAACTGCTTTCAGGCCAGATGCCGACGTGATCATCATGTC
AGGCGATGTGAGTGAATGCATCGCGCAGATTCCCGATCGTTCAATCGCCCTGATCGTCACATCACCGCCGTACAATCTGG
GAAAAGCCTATGAAGATCGAGTCTCGATTGAAGCATATTTGCAGACTCAGGCGGAACTTATTGCGCAGCTCTGTCGTGTG
TTGCGCGACGATGGCAGTATTTGCTGGCAGGTAGGTAACTTCGTCGAGCATGGTGAAGTGTACCCACTTGATATTCTGTA
CTACCCCATCTTCAAGAGACTGGGCCTGAGACTACGCAACCGGATTGTCTGGAAATTTGGGCATGGCTTGCACGCTTCAA
AACGATTCTCCGGGCGCTATGAAACGATCCTCTGGTTTACCAAATCGGACCACTACATCTTCAATTTAGATGCAGTTCGT
GTTCCGGCCAAATACCCCGGTAAACGACACTTTAAAGGTCCGAATAAGGGCAGGCCATCAGGTAATCCCCTGGGGAAGAA
TCCGTCGGATGTTTGGGAAATCCTCGAACGGGATTGGGAAGAACTGGTATGGGACATCCCGAATGTCAAATCGAACCATC
CAGAAAAGACCATACATCCCTGTCAGTTTCCGATTGAACTGGTTGAGCGTTGCGTGCTTGCCCTGACCAATGAAGGTGAT
ATGGTCTTTGACCCGTATATGGGGGTCGGCTCGACCTTGATTGCTGCATTGATGCATCAGCGACGTGCCGTTGGTTGTGA
TAAAGAAGCAACGTATGTCGAAATTGCTCGTCAACGTGTGGTTGATTATTTCAATGGTACCCTGCGTTACCGTCCGCTCG
GCAAACCCGTTTACCAGCCAACAGGGAGAGAGAAAGTTTCTCAGATTCCAGAGGAGTGGAAGGAGTCGTTACAAGCCCGA
CTTCTGGAGCGAAATGGAGATTACGAATGA

Upstream 100 bases:

>100_bases
GTTCAATTATTGTGCTACAATATGCTATACCATACCCTTCTACCGCGAATCAGGTTACAATGAGAAAACGGTATGTACCG
TGATTGATATAGGACAGGAT

Downstream 100 bases:

>100_bases
GAGTCGTCGGCAAATATTCTTTCAACGACGGTGAACGGTTTATCGAGGAGAACTATCCGATTGAATTGCAAGAGGTCTAC
AATGTTATAGAAAAAAGTAG

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.BglII; N(4)- cytosine-specific methyltransferase BglII [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSELPLFRSVTSIETAFRPDADVIIMSGDVSECIAQIPDRSIALIVTSPPYNLGKAYEDRVSIEAYLQTQAELIAQLCRV
LRDDGSICWQVGNFVEHGEVYPLDILYYPIFKRLGLRLRNRIVWKFGHGLHASKRFSGRYETILWFTKSDHYIFNLDAVR
VPAKYPGKRHFKGPNKGRPSGNPLGKNPSDVWEILERDWEELVWDIPNVKSNHPEKTIHPCQFPIELVERCVLALTNEGD
MVFDPYMGVGSTLIAALMHQRRAVGCDKEATYVEIARQRVVDYFNGTLRYRPLGKPVYQPTGREKVSQIPEEWKESLQAR
LLERNGDYE

Sequences:

>Translated_329_residues
MSELPLFRSVTSIETAFRPDADVIIMSGDVSECIAQIPDRSIALIVTSPPYNLGKAYEDRVSIEAYLQTQAELIAQLCRV
LRDDGSICWQVGNFVEHGEVYPLDILYYPIFKRLGLRLRNRIVWKFGHGLHASKRFSGRYETILWFTKSDHYIFNLDAVR
VPAKYPGKRHFKGPNKGRPSGNPLGKNPSDVWEILERDWEELVWDIPNVKSNHPEKTIHPCQFPIELVERCVLALTNEGD
MVFDPYMGVGSTLIAALMHQRRAVGCDKEATYVEIARQRVVDYFNGTLRYRPLGKPVYQPTGREKVSQIPEEWKESLQAR
LLERNGDYE
>Mature_328_residues
SELPLFRSVTSIETAFRPDADVIIMSGDVSECIAQIPDRSIALIVTSPPYNLGKAYEDRVSIEAYLQTQAELIAQLCRVL
RDDGSICWQVGNFVEHGEVYPLDILYYPIFKRLGLRLRNRIVWKFGHGLHASKRFSGRYETILWFTKSDHYIFNLDAVRV
PAKYPGKRHFKGPNKGRPSGNPLGKNPSDVWEILERDWEELVWDIPNVKSNHPEKTIHPCQFPIELVERCVLALTNEGDM
VFDPYMGVGSTLIAALMHQRRAVGCDKEATYVEIARQRVVDYFNGTLRYRPLGKPVYQPTGREKVSQIPEEWKESLQARL
LERNGDYE

Specific function: This methylase recognizes the double-stranded sequence AGATCT, causes specific methylation on C-5 on both strands, and protects the DNA from cleavage by the BglII endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=271, Percent_Identity=30.2583025830258, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 37799; Mature: 37667

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELPLFRSVTSIETAFRPDADVIIMSGDVSECIAQIPDRSIALIVTSPPYNLGKAYEDR
CCCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
VSIEAYLQTQAELIAQLCRVLRDDGSICWQVGNFVEHGEVYPLDILYYPIFKRLGLRLRN
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
RIVWKFGHGLHASKRFSGRYETILWFTKSDHYIFNLDAVRVPAKYPGKRHFKGPNKGRPS
HEEEEECCCCCCHHHCCCCEEEEEEEECCCCEEEEECEEECCCCCCCCCCCCCCCCCCCC
GNPLGKNPSDVWEILERDWEELVWDIPNVKSNHPEKTIHPCQFPIELVERCVLALTNEGD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
MVFDPYMGVGSTLIAALMHQRRAVGCDKEATYVEIARQRVVDYFNGTLRYRPLGKPVYQP
EEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
TGREKVSQIPEEWKESLQARLLERNGDYE
CCHHHHHHCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SELPLFRSVTSIETAFRPDADVIIMSGDVSECIAQIPDRSIALIVTSPPYNLGKAYEDR
CCCCHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHH
VSIEAYLQTQAELIAQLCRVLRDDGSICWQVGNFVEHGEVYPLDILYYPIFKRLGLRLRN
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHH
RIVWKFGHGLHASKRFSGRYETILWFTKSDHYIFNLDAVRVPAKYPGKRHFKGPNKGRPS
HEEEEECCCCCCHHHCCCCEEEEEEEECCCCEEEEECEEECCCCCCCCCCCCCCCCCCCC
GNPLGKNPSDVWEILERDWEELVWDIPNVKSNHPEKTIHPCQFPIELVERCVLALTNEGD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
MVFDPYMGVGSTLIAALMHQRRAVGCDKEATYVEIARQRVVDYFNGTLRYRPLGKPVYQP
EEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCC
TGREKVSQIPEEWKESLQARLLERNGDYE
CCHHHHHHCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9073062 [H]