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The map label for this gene is ygcA [C]

Identifier: 222524630

GI number: 222524630

Start: 1730821

End: 1732071

Strand: Direct

Name: ygcA [C]

Synonym: Chy400_1354

Alternate gene names: 222524630

Gene position: 1730821-1732071 (Clockwise)

Preceding gene: 222524629

Following gene: 222524631

Centisome position: 32.85

GC content: 57.63

Gene sequence:

>1251_bases
ATGCACGATACTATCGAACTTGAGATTGAAGGTATTGCCCAGGGTGGCGATGGCGTAGGCAGGCATGGCGATCTGGTCGT
GTTTGTCACCGGAGCACTACCGGGTGAGCGGGTCAGAGCAACCATCACCGAGCGCCACACCCGGTTTCTGCGGGCTACTG
TGTGTGACATTCTGCGCGCCGCTCCAGAGCGAGTTGAACCCCAGATCAAGCCTGGTGATCACGCACCGTGGCAACACATT
GCCTATCCGGCTCAAGTGCGTTACAAAGGGATCATCGTCGCAGACCAGCTACGCAAGTTTCTCCCCGACATCACCATACC
GATCCAGCCCGTAATTGCGGCACCGCATCCCTGGGGCTATCGCAATAGTGCCCGTCTGCATGTAGACGGAGCGACTCTTG
GTTATCACGCAGCCGGGACGAAGACCGTCGTTGATCTGGCAGATGATCCACTACTTTTACCGGCACTACGGGATACGCTG
CGGAGTTTGCGCCGGTTAGCCTTTGCCGGTTTAATCAAAGAGGTTACGCTGCGGGCCAGTGCAACCTACGGCTATGCCGC
TGCTCGCCTCTCGCCGACGCCAGATGCCGAACGACACGCGCTGTGGCGGCTGGCCACAGCCTGGAAGGCATCAACCCCTG
TGCTGGCAGGAGTCACGCTGGAAGATGAACCATTCGTGGGAACGCCCTTTGTGTACGACGAACTTGCCGGCGTGATCTTT
CGGTTGAGTCTGGGCAGCTTCTTCCAGGTCAATCATGCCCAGGCCGAGCGCATGGTTGCAGTCGTTCGTGACGCACTGGC
CCCGCATGGCGGTCGTCTCCTCGACGCCTACAGTGGGGTGGGGAGCTTTGCCCTGCCACTGGCCAGATGCTACGATGAGG
TAATTGCCGTTGAAGCCTATCAACCGGCGGTAGAGGATGGTCGGCAGAGCGCCATTGACAACCAGATTACAAATGTGCAA
TTTATCAACGGCGCTGCCGAGCACACTGTTGCCCATCTCAGCGGACCTTTTCACGCCGTCATTCTCGATCCGCCGCGTCG
TGGGTGTCATCCGGCCCTCATCGAGGCGATTATCGCTCATGCACCACCCCAAATCGTCTACATTTCGTGTCATCCCGGCC
TGATCGGGCGCGATCTCGCTCCACTACTGGCCGCCGGTTATCAGATCACATTCGTGCAGCCAGTCGATCTCTTTCCACAA
ACCCCACACATTGAAACAATTATCCATTTACGATCTGTTGTAGGAGAATAA

Upstream 100 bases:

>100_bases
TGATTGAGAAGTATTTGTAAAGTTACGGCACAGTAAATGGTTCAATCATTGTTCCCACGCTGCACAACACAAACTGTTGG
TATTACTCGTAGAGATACAT

Downstream 100 bases:

>100_bases
GATGGATTATTTTTAGTTTTTGGAATGATTAATCTGTAACCTGCTATCTATGTGATAATTGACAGAAACAATCATGTGTT
ATAATATCTGAAACAACAAT

Product: TrmA family RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MHDTIELEIEGIAQGGDGVGRHGDLVVFVTGALPGERVRATITERHTRFLRATVCDILRAAPERVEPQIKPGDHAPWQHI
AYPAQVRYKGIIVADQLRKFLPDITIPIQPVIAAPHPWGYRNSARLHVDGATLGYHAAGTKTVVDLADDPLLLPALRDTL
RSLRRLAFAGLIKEVTLRASATYGYAAARLSPTPDAERHALWRLATAWKASTPVLAGVTLEDEPFVGTPFVYDELAGVIF
RLSLGSFFQVNHAQAERMVAVVRDALAPHGGRLLDAYSGVGSFALPLARCYDEVIAVEAYQPAVEDGRQSAIDNQITNVQ
FINGAAEHTVAHLSGPFHAVILDPPRRGCHPALIEAIIAHAPPQIVYISCHPGLIGRDLAPLLAAGYQITFVQPVDLFPQ
TPHIETIIHLRSVVGE

Sequences:

>Translated_416_residues
MHDTIELEIEGIAQGGDGVGRHGDLVVFVTGALPGERVRATITERHTRFLRATVCDILRAAPERVEPQIKPGDHAPWQHI
AYPAQVRYKGIIVADQLRKFLPDITIPIQPVIAAPHPWGYRNSARLHVDGATLGYHAAGTKTVVDLADDPLLLPALRDTL
RSLRRLAFAGLIKEVTLRASATYGYAAARLSPTPDAERHALWRLATAWKASTPVLAGVTLEDEPFVGTPFVYDELAGVIF
RLSLGSFFQVNHAQAERMVAVVRDALAPHGGRLLDAYSGVGSFALPLARCYDEVIAVEAYQPAVEDGRQSAIDNQITNVQ
FINGAAEHTVAHLSGPFHAVILDPPRRGCHPALIEAIIAHAPPQIVYISCHPGLIGRDLAPLLAAGYQITFVQPVDLFPQ
TPHIETIIHLRSVVGE
>Mature_416_residues
MHDTIELEIEGIAQGGDGVGRHGDLVVFVTGALPGERVRATITERHTRFLRATVCDILRAAPERVEPQIKPGDHAPWQHI
AYPAQVRYKGIIVADQLRKFLPDITIPIQPVIAAPHPWGYRNSARLHVDGATLGYHAAGTKTVVDLADDPLLLPALRDTL
RSLRRLAFAGLIKEVTLRASATYGYAAARLSPTPDAERHALWRLATAWKASTPVLAGVTLEDEPFVGTPFVYDELAGVIF
RLSLGSFFQVNHAQAERMVAVVRDALAPHGGRLLDAYSGVGSFALPLARCYDEVIAVEAYQPAVEDGRQSAIDNQITNVQ
FINGAAEHTVAHLSGPFHAVILDPPRRGCHPALIEAIIAHAPPQIVYISCHPGLIGRDLAPLLAAGYQITFVQPVDLFPQ
TPHIETIIHLRSVVGE

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=368, Percent_Identity=29.8913043478261, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI51173878, Length=368, Percent_Identity=29.8913043478261, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI269784680, Length=202, Percent_Identity=32.1782178217822, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI269784676, Length=202, Percent_Identity=32.1782178217822, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI20149688, Length=202, Percent_Identity=32.1782178217822, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI269784678, Length=202, Percent_Identity=32.1782178217822, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1789148, Length=424, Percent_Identity=29.9528301886792, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1790403, Length=183, Percent_Identity=31.1475409836066, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787083, Length=325, Percent_Identity=28, Blast_Score=81, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322909, Length=410, Percent_Identity=23.9024390243902, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24666579, Length=459, Percent_Identity=25.0544662309368, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 45171; Mature: 45171

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDTIELEIEGIAQGGDGVGRHGDLVVFVTGALPGERVRATITERHTRFLRATVCDILRA
CCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
APERVEPQIKPGDHAPWQHIAYPAQVRYKGIIVADQLRKFLPDITIPIQPVIAAPHPWGY
CHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEHHHHHHHCCCCCCCCCEEEECCCCCCC
RNSARLHVDGATLGYHAAGTKTVVDLADDPLLLPALRDTLRSLRRLAFAGLIKEVTLRAS
CCCCEEEECCCEEEEECCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ATYGYAAARLSPTPDAERHALWRLATAWKASTPVLAGVTLEDEPFVGTPFVYDELAGVIF
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
RLSLGSFFQVNHAQAERMVAVVRDALAPHGGRLLDAYSGVGSFALPLARCYDEVIAVEAY
HHCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHCC
QPAVEDGRQSAIDNQITNVQFINGAAEHTVAHLSGPFHAVILDPPRRGCHPALIEAIIAH
CCHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHC
APPQIVYISCHPGLIGRDLAPLLAAGYQITFVQPVDLFPQTPHIETIIHLRSVVGE
CCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHDTIELEIEGIAQGGDGVGRHGDLVVFVTGALPGERVRATITERHTRFLRATVCDILRA
CCCEEEEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
APERVEPQIKPGDHAPWQHIAYPAQVRYKGIIVADQLRKFLPDITIPIQPVIAAPHPWGY
CHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEHHHHHHHCCCCCCCCCEEEECCCCCCC
RNSARLHVDGATLGYHAAGTKTVVDLADDPLLLPALRDTLRSLRRLAFAGLIKEVTLRAS
CCCCEEEECCCEEEEECCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ATYGYAAARLSPTPDAERHALWRLATAWKASTPVLAGVTLEDEPFVGTPFVYDELAGVIF
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
RLSLGSFFQVNHAQAERMVAVVRDALAPHGGRLLDAYSGVGSFALPLARCYDEVIAVEAY
HHCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCHHHHHHHHHHHHHHHHHCC
QPAVEDGRQSAIDNQITNVQFINGAAEHTVAHLSGPFHAVILDPPRRGCHPALIEAIIAH
CCHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHC
APPQIVYISCHPGLIGRDLAPLLAAGYQITFVQPVDLFPQTPHIETIIHLRSVVGE
CCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]