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The map label for this gene is aam1 [H]

Identifier: 222524546

GI number: 222524546

Start: 1618717

End: 1620756

Strand: Direct

Name: aam1 [H]

Synonym: Chy400_1270

Alternate gene names: 222524546

Gene position: 1618717-1620756 (Clockwise)

Preceding gene: 222524545

Following gene: 222524547

Centisome position: 30.72

GC content: 56.08

Gene sequence:

>2040_bases
ATGACCGTCAAGGAGTCACGCACTCGTACTCGCCGCACGGCGGCAAAAGCGAAACTCGATCTATCGGTGATCGGTGAAGG
ACGGCGACGGGTCATTATTGAAGCGGTTGAGCCAATCATTGATGGTGGTCGTTATCCGGTGAAGCGAATCATCGGTGATA
CGCTGGTGGTCAGTTGCGATCTGTTTGCCGATGGTCATGATGAACTGGCAGCAGTAGTACGCCATCGACCGGTCAGCGCT
AAGACATGGCATGAAGCACCAATGCGCTTACTGGTCAATGATCGTTGGGAAGGTCAGTTTACCCTGAATAGCATCGGTCG
GCATGAGTTTCAGGTTGTGGCCTGGATCGACCGTTTTGCGACCTGGGTGCATCAACTTGAGAAGCGGTTGGCTGCCGGGC
AAGATGTGCGGGTCGATTTGCAGATCGGTGCCGCGCTGGTTGGTGAGGCTGCACGCAATGCCAGTGGCGCTGATGCTGCG
GTGCTGCATGTCGCCGAAGCGGCCCTGATTGCCGGTGACGTTGATACAGCCTTTGCCGCTGAAATCCACTCCTTGATGGC
TGTCCATTTGCCACGCCGATTTGCTACCGAGTCTGAGATTTACCCGATAACAGTCGAACGCGAACGAGCACGCTTTTCCG
CCTGGTACGAGCTGTTTCCCCGCTCGGCGGCTGACGAGCCGGGTCGCCACGGCACGTTCCGCGATGTGATCAAGCGGCTG
CCGTACATTGCCGAACTCGGTTTTGATGTGCTGTACCTGCCACCGATCCATCCGATTGGATATACCTTCCGCAAGGGCAA
AAATAACAGCCTGAATGCCGGCCCCAACGATCCGGGCAGCCCATGGGCGATTGGGAATGCCGATGGCGGGCATATGAGCA
TTCATCCCGAATTGGGCACATTGGATGATTTTCGGGCGCTGGTGCGCGAAGCGCAGAAATACGGTATCGAAGTAGCACTT
GACATCGCGTTCCAATGCTCACCCGATCATCCGTACGTGCGTGAGCATCCAGAGTGGTTCCGCGCTCGCCCAGACGGAAC
CATCCAGTATGCCGAGAATCCACCGAAAAAATATCAGGATATCTACCCGTTCGATTTTGAATCGGAAGACTGGCAGGGGC
TATGGCAAGAGCTGCGCCGGGTCTTCGAGTTCTGGATCGAGCAGGGTGTGCGTATCTTTCGGGTGGATAACCCGCATACC
AAGAGCTTTCGCTTCTGGGACTGGTGTCTGAACTCGCTGAAGAGTGCGCATCCCGACCTGATCTTTCTCTCGGAAGCGTT
CACCCGGCCAAAGGTGATGTACCATCTGGCCAAGATTGGCTTTACGCAGAGCTATACCTATTACACCTGGCGCACTACCA
AACACGAGTTGACCAGCTATCTGCAGGAATTGAATACCCGCCCGGTCAGTGATTTCTTCATTCCCAATTTCTGGCCGAAT
ACGCCTGACATTCTGACCCCACAGTTTTACGCTGGTCAGCGCTCGGTGTTTATGACCCGCGCTGCGATGGCGGCGACGTT
GACGGCCAGTTGGGGTATGTACGGCCCGGTGTATGAATTGATGGAGCACACGCCGGTAGCCGGACGTGAAGAGTACATTA
ACAATGAGAAGTACGAAATCCGGCACTGGAATCTCGATGCACCGCACAGCCTGCGCGGCTATATCGGCCAGCTCAATCGG
ATTCGGCGTGAGCATCCGGCCCTTCACCGGAATGACACCCTGCGCTTCCACCGCGTAGACATCGATTTTCACGAGCACGA
ATGGTTGCTCGCGTATAGCAAGACATCACCGGACGGCAGCGACATCATTCTGGTGGTGGTCAACCTTGACCCCGAACGTA
CCCACAGTGGTTGGGTTCAGGTGCCGCTGAACGACTGGAACGTGAATGGCGTGTTCCAGGCCCACGATCTGTTGACGGAC
GCACGTTACACCTGGTCGGGAGAGTATAATTACGTGCAGCTCAGCCCGATGGCTCCCGTCCATATCTTTTGTATTCGCCG
GCTCCGCCGTGACGAGCGCGGCTTTGAGTATTTCGCCTGA

Upstream 100 bases:

>100_bases
TTATCAATACCCCAGCATACGACGTACCGACCTATACGGTTTGTCGTCTATCGCAAGTGCACAGAACGCACATTGCTTGC
TGTCAAATTGAGGAGGTCTA

Downstream 100 bases:

>100_bases
TAGTTGTACGATGAGGGAGTGGCATGAACCGTGTACGCCCGGCCAAGACGCCGACTCTGTTAGCCGATGATCCGCTCTGG
TACAAAGATGCGGTCATCTA

Product: alpha amylase catalytic subunit

Products: D-Glucose 6-phosphate; D-Glucose [C]

Alternate protein names: NA

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MTVKESRTRTRRTAAKAKLDLSVIGEGRRRVIIEAVEPIIDGGRYPVKRIIGDTLVVSCDLFADGHDELAAVVRHRPVSA
KTWHEAPMRLLVNDRWEGQFTLNSIGRHEFQVVAWIDRFATWVHQLEKRLAAGQDVRVDLQIGAALVGEAARNASGADAA
VLHVAEAALIAGDVDTAFAAEIHSLMAVHLPRRFATESEIYPITVERERARFSAWYELFPRSAADEPGRHGTFRDVIKRL
PYIAELGFDVLYLPPIHPIGYTFRKGKNNSLNAGPNDPGSPWAIGNADGGHMSIHPELGTLDDFRALVREAQKYGIEVAL
DIAFQCSPDHPYVREHPEWFRARPDGTIQYAENPPKKYQDIYPFDFESEDWQGLWQELRRVFEFWIEQGVRIFRVDNPHT
KSFRFWDWCLNSLKSAHPDLIFLSEAFTRPKVMYHLAKIGFTQSYTYYTWRTTKHELTSYLQELNTRPVSDFFIPNFWPN
TPDILTPQFYAGQRSVFMTRAAMAATLTASWGMYGPVYELMEHTPVAGREEYINNEKYEIRHWNLDAPHSLRGYIGQLNR
IRREHPALHRNDTLRFHRVDIDFHEHEWLLAYSKTSPDGSDIILVVVNLDPERTHSGWVQVPLNDWNVNGVFQAHDLLTD
ARYTWSGEYNYVQLSPMAPVHIFCIRRLRRDERGFEYFA

Sequences:

>Translated_679_residues
MTVKESRTRTRRTAAKAKLDLSVIGEGRRRVIIEAVEPIIDGGRYPVKRIIGDTLVVSCDLFADGHDELAAVVRHRPVSA
KTWHEAPMRLLVNDRWEGQFTLNSIGRHEFQVVAWIDRFATWVHQLEKRLAAGQDVRVDLQIGAALVGEAARNASGADAA
VLHVAEAALIAGDVDTAFAAEIHSLMAVHLPRRFATESEIYPITVERERARFSAWYELFPRSAADEPGRHGTFRDVIKRL
PYIAELGFDVLYLPPIHPIGYTFRKGKNNSLNAGPNDPGSPWAIGNADGGHMSIHPELGTLDDFRALVREAQKYGIEVAL
DIAFQCSPDHPYVREHPEWFRARPDGTIQYAENPPKKYQDIYPFDFESEDWQGLWQELRRVFEFWIEQGVRIFRVDNPHT
KSFRFWDWCLNSLKSAHPDLIFLSEAFTRPKVMYHLAKIGFTQSYTYYTWRTTKHELTSYLQELNTRPVSDFFIPNFWPN
TPDILTPQFYAGQRSVFMTRAAMAATLTASWGMYGPVYELMEHTPVAGREEYINNEKYEIRHWNLDAPHSLRGYIGQLNR
IRREHPALHRNDTLRFHRVDIDFHEHEWLLAYSKTSPDGSDIILVVVNLDPERTHSGWVQVPLNDWNVNGVFQAHDLLTD
ARYTWSGEYNYVQLSPMAPVHIFCIRRLRRDERGFEYFA
>Mature_678_residues
TVKESRTRTRRTAAKAKLDLSVIGEGRRRVIIEAVEPIIDGGRYPVKRIIGDTLVVSCDLFADGHDELAAVVRHRPVSAK
TWHEAPMRLLVNDRWEGQFTLNSIGRHEFQVVAWIDRFATWVHQLEKRLAAGQDVRVDLQIGAALVGEAARNASGADAAV
LHVAEAALIAGDVDTAFAAEIHSLMAVHLPRRFATESEIYPITVERERARFSAWYELFPRSAADEPGRHGTFRDVIKRLP
YIAELGFDVLYLPPIHPIGYTFRKGKNNSLNAGPNDPGSPWAIGNADGGHMSIHPELGTLDDFRALVREAQKYGIEVALD
IAFQCSPDHPYVREHPEWFRARPDGTIQYAENPPKKYQDIYPFDFESEDWQGLWQELRRVFEFWIEQGVRIFRVDNPHTK
SFRFWDWCLNSLKSAHPDLIFLSEAFTRPKVMYHLAKIGFTQSYTYYTWRTTKHELTSYLQELNTRPVSDFFIPNFWPNT
PDILTPQFYAGQRSVFMTRAAMAATLTASWGMYGPVYELMEHTPVAGREEYINNEKYEIRHWNLDAPHSLRGYIGQLNRI
RREHPALHRNDTLRFHRVDIDFHEHEWLLAYSKTSPDGSDIILVVVNLDPERTHSGWVQVPLNDWNVNGVFQAHDLLTDA
RYTWSGEYNYVQLSPMAPVHIFCIRRLRRDERGFEYFA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.93 [C]

Molecular weight: Translated: 78338; Mature: 78207

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKESRTRTRRTAAKAKLDLSVIGEGRRRVIIEAVEPIIDGGRYPVKRIIGDTLVVSCD
CCCCCHHHHHHHHHHHHHEEEEEECCCCCEEHHHHHHHHHCCCCCCHHHHCCCEEEEEEE
LFADGHDELAAVVRHRPVSAKTWHEAPMRLLVNDRWEGQFTLNSIGRHEFQVVAWIDRFA
ECCCCHHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHH
TWVHQLEKRLAAGQDVRVDLQIGAALVGEAARNASGADAAVLHVAEAALIAGDVDTAFAA
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCHHHHH
EIHSLMAVHLPRRFATESEIYPITVERERARFSAWYELFPRSAADEPGRHGTFRDVIKRL
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHC
PYIAELGFDVLYLPPIHPIGYTFRKGKNNSLNAGPNDPGSPWAIGNADGGHMSIHPELGT
CHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCC
LDDFRALVREAQKYGIEVALDIAFQCSPDHPYVREHPEWFRARPDGTIQYAENPPKKYQD
HHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHCCCCCEEEECCCCCHHHCC
IYPFDFESEDWQGLWQELRRVFEFWIEQGVRIFRVDNPHTKSFRFWDWCLNSLKSAHPDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCE
IFLSEAFTRPKVMYHLAKIGFTQSYTYYTWRTTKHELTSYLQELNTRPVSDFFIPNFWPN
EEECHHHCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHCCCCCCCCC
TPDILTPQFYAGQRSVFMTRAAMAATLTASWGMYGPVYELMEHTPVAGREEYINNEKYEI
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHCCCCCEEE
RHWNLDAPHSLRGYIGQLNRIRREHPALHRNDTLRFHRVDIDFHEHEWLLAYSKTSPDGS
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCEEEEEEECCCCCCC
DIILVVVNLDPERTHSGWVQVPLNDWNVNGVFQAHDLLTDARYTWSGEYNYVQLSPMAPV
CEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEHHHHHHCCEEEECCCCCEEEECCCCCH
HIFCIRRLRRDERGFEYFA
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TVKESRTRTRRTAAKAKLDLSVIGEGRRRVIIEAVEPIIDGGRYPVKRIIGDTLVVSCD
CCCCHHHHHHHHHHHHHEEEEEECCCCCEEHHHHHHHHHCCCCCCHHHHCCCEEEEEEE
LFADGHDELAAVVRHRPVSAKTWHEAPMRLLVNDRWEGQFTLNSIGRHEFQVVAWIDRFA
ECCCCHHHHHHHHHCCCCCCCCHHHCCCEEEECCCCCCEEECCCCCCCHHHHHHHHHHHH
TWVHQLEKRLAAGQDVRVDLQIGAALVGEAARNASGADAAVLHVAEAALIAGDVDTAFAA
HHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCCHHHHHHHHHHHEECCCCHHHHH
EIHSLMAVHLPRRFATESEIYPITVERERARFSAWYELFPRSAADEPGRHGTFRDVIKRL
HHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHC
PYIAELGFDVLYLPPIHPIGYTFRKGKNNSLNAGPNDPGSPWAIGNADGGHMSIHPELGT
CHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCEEEECCCCCC
LDDFRALVREAQKYGIEVALDIAFQCSPDHPYVREHPEWFRARPDGTIQYAENPPKKYQD
HHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCHHHHCCCCCEEEECCCCCHHHCC
IYPFDFESEDWQGLWQELRRVFEFWIEQGVRIFRVDNPHTKSFRFWDWCLNSLKSAHPDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCE
IFLSEAFTRPKVMYHLAKIGFTQSYTYYTWRTTKHELTSYLQELNTRPVSDFFIPNFWPN
EEECHHHCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHCCCCCCCCC
TPDILTPQFYAGQRSVFMTRAAMAATLTASWGMYGPVYELMEHTPVAGREEYINNEKYEI
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHCCCCCEEE
RHWNLDAPHSLRGYIGQLNRIRREHPALHRNDTLRFHRVDIDFHEHEWLLAYSKTSPDGS
EEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCEEEEEEECCCCCCC
DIILVVVNLDPERTHSGWVQVPLNDWNVNGVFQAHDLLTDARYTWSGEYNYVQLSPMAPV
CEEEEEEECCCCCCCCCEEEEECCCCCCCCEEEHHHHHHCCEEEECCCCCEEEECCCCCH
HIFCIRRLRRDERGFEYFA
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; alpha,alpha'-Trehalose 6-phosphate [C]

Specific reaction: H2O + alpha,alpha'-Trehalose 6-phosphate --> D-Glucose 6-phosphate + D-Glucose [C]

General reaction: O-Glycosyl bond hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10970853 [H]