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The map label for this gene is glnA [H]

Identifier: 222524195

GI number: 222524195

Start: 1156258

End: 1157682

Strand: Reverse

Name: glnA [H]

Synonym: Chy400_0916

Alternate gene names: 222524195

Gene position: 1157682-1156258 (Counterclockwise)

Preceding gene: 222524197

Following gene: 222524193

Centisome position: 21.97

GC content: 56.98

Gene sequence:

>1425_bases
ATGACCGAGCTACGCGACTTTCTCGAAATCCCCTACGAGCAACTGGAAGAGCTGAATCTGAGTGTTAAGCGGCAGCGTCT
GGCCCGCGTTTCGCCGGATGTGATGCGCGAGGAGCGGATGCGCTATTTGACCGACGAGCGGCGGATTAAGGCGGTGACCG
TCTGTTTTACCGACCTCGAAGGCCGGCTGCATATGCTCGATTACGACAAGAAGTTTCTGCTGTCCTCCGCCGATAATCTG
ACCTTTGACGGTTCGTCGGTGCGAGGCTTTTCGGTGCAGGCCGAGTCGGATTTGCGCCTGGCGATTGACTGGTCGGCTTT
TTACTGGTTGCCGGCAGATATTTTTGGCCCCGGTAAGGTGCTGGTCTTTGGGTTTGTGTTGAATCAGGACGGTAGCCCCT
ACGAGGCTGATATGCGGGGGCGGTTGCACCGGTATGTGAATGAGCTGTATCAGCGCGATGGGCTGACCTGCAATGTGGCT
GCTGAGATTGAGGGCTTCCTGTTTAAGGGGCGCAATGCCGAGCAGCGCTTCCGCGAGACCGGTGAGTTTACGATGGTCAG
TACCGGTGGTTATTACCACTCGTTGCCGGGTGATCCGCTGCGCCAGTTCATCGATATGGCTGCCGAAGCGCAGCGGGCGA
TGGGGTTTGAGAATGAGAAGGATCACCCTGAAGTGGCGCCGTCACAGTTTGAGATGAATTATAGCTATACCGAGGCAACT
ATCGCCGCTGACCAGATTCTGCTTTATAAGTTGATCTGTCGCCAGGTGGCGAGCAAGCTGGATATGACGGCCTGCTTCCT
GCCGAAGCCGGTGACCGGGGTGAATGGCAATGGCATGCACACCAATATCTCGTTGTCGCGCCACGGCGAGAATCTCTTCT
ACGATGCGAATGGCCCGGAACGGCTTTCCGAGCTGGCCTGGAGCTTTATTGACCGTATTCTGAACAGTGGTCAGGAACTC
TGCCTGATCCTGAATTCGAGCGTCAATGCCTATCGCCGGCTAGACCCACACTTCGAGGCACCAAACCAGATCAAGGCGTC
ACCTACCGACCGCGGTTCGATGATCCGGATTCCGATTGGTAATGCCCGTTCGGCCCGGATTGAAGTGCGCTCGATTGCGC
CCGATGCCAATCCCTATATGGCGATTTATGCGCTGCTGCGCACCGGTCTCGAAGGGCCGCTGCCGACGACCGATGTTCGC
AACGGCACAGCAACGCTTCCCGATAACATCTACGACGCTATCACCAATTTCCGCCAGAGCACCTTCCTGGCCAGCATTCT
CGGTGAAGGGGTGCACAGCCGTTTCGCCGACCTGAAGACAACCGTCGCCGACCGTTGTCCACGCAAGCTCGGTACGTTGA
TCAAACGCGCCGAGGTGCAGTTCCACCACGAAGTCACCAATCAGTACCTGTGGAGCCGATTCTAA

Upstream 100 bases:

>100_bases
TCGGTTTGGTTGCAGGGTTTTGTCAACCGTTGCTGCTGTTCGGAGTATAATACGACCCAACAACGTTACCGTTGTCTGGA
GTAAAGAAAAGGAAGCCACC

Downstream 100 bases:

>100_bases
GCACCGGCCCCACCCCGAATCGACCTCGTCCGGTGAGGGAGCGCAATGGCGCGCGACCTCTTTACAATGCCACAATTGCC
GCTGCCAGTACCAGACAATA

Product: glutamine synthetase catalytic subunit

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTELRDFLEIPYEQLEELNLSVKRQRLARVSPDVMREERMRYLTDERRIKAVTVCFTDLEGRLHMLDYDKKFLLSSADNL
TFDGSSVRGFSVQAESDLRLAIDWSAFYWLPADIFGPGKVLVFGFVLNQDGSPYEADMRGRLHRYVNELYQRDGLTCNVA
AEIEGFLFKGRNAEQRFRETGEFTMVSTGGYYHSLPGDPLRQFIDMAAEAQRAMGFENEKDHPEVAPSQFEMNYSYTEAT
IAADQILLYKLICRQVASKLDMTACFLPKPVTGVNGNGMHTNISLSRHGENLFYDANGPERLSELAWSFIDRILNSGQEL
CLILNSSVNAYRRLDPHFEAPNQIKASPTDRGSMIRIPIGNARSARIEVRSIAPDANPYMAIYALLRTGLEGPLPTTDVR
NGTATLPDNIYDAITNFRQSTFLASILGEGVHSRFADLKTTVADRCPRKLGTLIKRAEVQFHHEVTNQYLWSRF

Sequences:

>Translated_474_residues
MTELRDFLEIPYEQLEELNLSVKRQRLARVSPDVMREERMRYLTDERRIKAVTVCFTDLEGRLHMLDYDKKFLLSSADNL
TFDGSSVRGFSVQAESDLRLAIDWSAFYWLPADIFGPGKVLVFGFVLNQDGSPYEADMRGRLHRYVNELYQRDGLTCNVA
AEIEGFLFKGRNAEQRFRETGEFTMVSTGGYYHSLPGDPLRQFIDMAAEAQRAMGFENEKDHPEVAPSQFEMNYSYTEAT
IAADQILLYKLICRQVASKLDMTACFLPKPVTGVNGNGMHTNISLSRHGENLFYDANGPERLSELAWSFIDRILNSGQEL
CLILNSSVNAYRRLDPHFEAPNQIKASPTDRGSMIRIPIGNARSARIEVRSIAPDANPYMAIYALLRTGLEGPLPTTDVR
NGTATLPDNIYDAITNFRQSTFLASILGEGVHSRFADLKTTVADRCPRKLGTLIKRAEVQFHHEVTNQYLWSRF
>Mature_473_residues
TELRDFLEIPYEQLEELNLSVKRQRLARVSPDVMREERMRYLTDERRIKAVTVCFTDLEGRLHMLDYDKKFLLSSADNLT
FDGSSVRGFSVQAESDLRLAIDWSAFYWLPADIFGPGKVLVFGFVLNQDGSPYEADMRGRLHRYVNELYQRDGLTCNVAA
EIEGFLFKGRNAEQRFRETGEFTMVSTGGYYHSLPGDPLRQFIDMAAEAQRAMGFENEKDHPEVAPSQFEMNYSYTEATI
AADQILLYKLICRQVASKLDMTACFLPKPVTGVNGNGMHTNISLSRHGENLFYDANGPERLSELAWSFIDRILNSGQELC
LILNSSVNAYRRLDPHFEAPNQIKASPTDRGSMIRIPIGNARSARIEVRSIAPDANPYMAIYALLRTGLEGPLPTTDVRN
GTATLPDNIYDAITNFRQSTFLASILGEGVHSRFADLKTTVADRCPRKLGTLIKRAEVQFHHEVTNQYLWSRF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=419, Percent_Identity=29.1169451073986, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI87081870, Length=353, Percent_Identity=28.6118980169972, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53821; Mature: 53689

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELRDFLEIPYEQLEELNLSVKRQRLARVSPDVMREERMRYLTDERRIKAVTVCFTDLE
CCCHHHHHHCCHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECCC
GRLHMLDYDKKFLLSSADNLTFDGSSVRGFSVQAESDLRLAIDWSAFYWLPADIFGPGKV
CEEEEECCCHHHHHCCCCCCEECCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCEE
LVFGFVLNQDGSPYEADMRGRLHRYVNELYQRDGLTCNVAAEIEGFLFKGRNAEQRFRET
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHC
GEFTMVSTGGYYHSLPGDPLRQFIDMAAEAQRAMGFENEKDHPEVAPSQFEMNYSYTEAT
CCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHH
IAADQILLYKLICRQVASKLDMTACFLPKPVTGVNGNGMHTNISLSRHGENLFYDANGPE
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEEEECCCEEEECCCCHH
RLSELAWSFIDRILNSGQELCLILNSSVNAYRRLDPHFEAPNQIKASPTDRGSMIRIPIG
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECC
NARSARIEVRSIAPDANPYMAIYALLRTGLEGPLPTTDVRNGTATLPDNIYDAITNFRQS
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TFLASILGEGVHSRFADLKTTVADRCPRKLGTLIKRAEVQFHHEVTNQYLWSRF
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TELRDFLEIPYEQLEELNLSVKRQRLARVSPDVMREERMRYLTDERRIKAVTVCFTDLE
CCHHHHHHCCHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEEEEECCC
GRLHMLDYDKKFLLSSADNLTFDGSSVRGFSVQAESDLRLAIDWSAFYWLPADIFGPGKV
CEEEEECCCHHHHHCCCCCCEECCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCEE
LVFGFVLNQDGSPYEADMRGRLHRYVNELYQRDGLTCNVAAEIEGFLFKGRNAEQRFRET
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCHHHHHHHC
GEFTMVSTGGYYHSLPGDPLRQFIDMAAEAQRAMGFENEKDHPEVAPSQFEMNYSYTEAT
CCEEEEECCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHH
IAADQILLYKLICRQVASKLDMTACFLPKPVTGVNGNGMHTNISLSRHGENLFYDANGPE
HHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEEEECCCEEEECCCCHH
RLSELAWSFIDRILNSGQELCLILNSSVNAYRRLDPHFEAPNQIKASPTDRGSMIRIPIG
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECC
NARSARIEVRSIAPDANPYMAIYALLRTGLEGPLPTTDVRNGTATLPDNIYDAITNFRQS
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TFLASILGEGVHSRFADLKTTVADRCPRKLGTLIKRAEVQFHHEVTNQYLWSRF
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2906311; 2565853; 9384377; 11719184 [H]