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The map label for this gene is lutB [H]

Identifier: 222524175

GI number: 222524175

Start: 1127096

End: 1128523

Strand: Reverse

Name: lutB [H]

Synonym: Chy400_0894

Alternate gene names: 222524175

Gene position: 1128523-1127096 (Counterclockwise)

Preceding gene: 222524176

Following gene: 222524174

Centisome position: 21.42

GC content: 60.29

Gene sequence:

>1428_bases
ATGGCGCAGACGATTGCTTTCTATGATCGCGTTGATCATGCCCTGCACGATGAGTCGTTGCAGACGGCGTTGACGCGGGC
GACGACGCGCTTTGTCGGTAATCGCGCCGGCGCGATTGGTGCGTTGAGTGATGCCGACCGGCTGCGCGATCAGGCGCGGG
CGATTCGGGCGCATGCGTTGGCCCATCTCGATGAATTGCTGCCGCAACTGGCGGCGAATGTTGAAGCGCGAGGCGGGCAT
GTGTGCTGGGCCGCCGATGGTGAAGAGGCTCGCCGCTATATCATCGATCTGGCGCGTGCGCGTGGCGTGAAGAGTATTGT
GAAGTCGAAATCAATGGCCTCGGAAGAGATTCATCTCAACGAGGCGCTGGAGCACGCCGGTTTTGAGGTCGTCGAGACCG
ATCTGGGTGAGTATATTATTCAACTGGCCGGTGAGACGCCGTCGCATATTATTGCCCCTGCTATTCACAAGACGCGCGAG
CAGGTCAGTGAGCTGTTTGCGCAGCATCTCGGTATGCAGCCGACGACCGAAGTACCGCCGATGATCCGCACGGCCCGTCA
GGCGTTGCGCCAACGCTTTTTGCAGGCCGATATGGGGATCAGTGGGGTCAATTTTGGTGTGGCTGATAGCGGTGCGATTG
TGATTGTCGAGAACGAGGGGAATGCGCGTCTCTCGACGACGGTGCCCCGGATTCACGTGGCGATTATGGGTATTGAGCGC
ATTGTTGCCCGTCTCGCCGATCTGGGGGTGATGTTGCAGGTACTGGCGCGCTCGGCAACCGGCCAGAAGTTGAGTGTCTA
TACCAGTCTGATCAGCGGCCCGGCCCGCCCCGGTGAAGAGGACGGCCCTGAAGAGTTTCACCTGGTCTTGCTCGACAATG
GCCGCTCGCGGATTTTGGGTGGTCATTACGCCGAGTCGCTGCTCTGTATCCGCTGTGGTGCCTGCCTGAACGCCTGCCCG
GTCTATCAGGCGATTGGCGGTCACGCCTACGGTGGGGTCTATTCTGGCCCGATTGGGGCGATTCTTACGCCCCTGCTGCA
ACCCGATCTTCCCGACGCCTATCTGTTGCCGCAGGCCAGCTCGCTCTGCGGGGCGTGCCAGGAGGTTTGTCCGGTGCGGA
TCGCTATCCCCGATATGCTGTTACGCCACCGGGCCGATGCCGTGAAGGCGGGGAAGGCGAATCCGATTGAAAAAGCGGCG
ATTAGTGGCTATACCCTGACGATGACCCATCCGGCGCTGTATCGCGCCAGTGGCAAGGCGGCGCGTTTCTTTAGCCGGAT
TCTGGCGCGGAAAGGTCGTTTTCGTCGCTTGCCACCACCCTTGCATTTGTGGACGCAGCAACGCGATTTTCCTGCACCAC
CACCGAAAACGTTTCGTGAATTGTGGGCCGAACGCCAGAAGCAGCGCCGGAATGAGGAACAAGCCTGA

Upstream 100 bases:

>100_bases
GGAGGTTCTGGCGAATATGATTGATGCACGTCAGGGATGACGGTGTGTCAGGGTATTGCCTGACCGTCGCTCATCGCTGG
TGTGCCTGAAAGAAGACAGT

Downstream 100 bases:

>100_bases
TATGCGTATGGAGATCACCATCCAGGCCGAACGACCTGATAGCAGTGATGCCCTGGCTCTGATCGGTGAACTGGAAGCGG
AGCTGGAACCGCTCTACCCA

Product: iron-sulfur cluster binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAQTIAFYDRVDHALHDESLQTALTRATTRFVGNRAGAIGALSDADRLRDQARAIRAHALAHLDELLPQLAANVEARGGH
VCWAADGEEARRYIIDLARARGVKSIVKSKSMASEEIHLNEALEHAGFEVVETDLGEYIIQLAGETPSHIIAPAIHKTRE
QVSELFAQHLGMQPTTEVPPMIRTARQALRQRFLQADMGISGVNFGVADSGAIVIVENEGNARLSTTVPRIHVAIMGIER
IVARLADLGVMLQVLARSATGQKLSVYTSLISGPARPGEEDGPEEFHLVLLDNGRSRILGGHYAESLLCIRCGACLNACP
VYQAIGGHAYGGVYSGPIGAILTPLLQPDLPDAYLLPQASSLCGACQEVCPVRIAIPDMLLRHRADAVKAGKANPIEKAA
ISGYTLTMTHPALYRASGKAARFFSRILARKGRFRRLPPPLHLWTQQRDFPAPPPKTFRELWAERQKQRRNEEQA

Sequences:

>Translated_475_residues
MAQTIAFYDRVDHALHDESLQTALTRATTRFVGNRAGAIGALSDADRLRDQARAIRAHALAHLDELLPQLAANVEARGGH
VCWAADGEEARRYIIDLARARGVKSIVKSKSMASEEIHLNEALEHAGFEVVETDLGEYIIQLAGETPSHIIAPAIHKTRE
QVSELFAQHLGMQPTTEVPPMIRTARQALRQRFLQADMGISGVNFGVADSGAIVIVENEGNARLSTTVPRIHVAIMGIER
IVARLADLGVMLQVLARSATGQKLSVYTSLISGPARPGEEDGPEEFHLVLLDNGRSRILGGHYAESLLCIRCGACLNACP
VYQAIGGHAYGGVYSGPIGAILTPLLQPDLPDAYLLPQASSLCGACQEVCPVRIAIPDMLLRHRADAVKAGKANPIEKAA
ISGYTLTMTHPALYRASGKAARFFSRILARKGRFRRLPPPLHLWTQQRDFPAPPPKTFRELWAERQKQRRNEEQA
>Mature_474_residues
AQTIAFYDRVDHALHDESLQTALTRATTRFVGNRAGAIGALSDADRLRDQARAIRAHALAHLDELLPQLAANVEARGGHV
CWAADGEEARRYIIDLARARGVKSIVKSKSMASEEIHLNEALEHAGFEVVETDLGEYIIQLAGETPSHIIAPAIHKTREQ
VSELFAQHLGMQPTTEVPPMIRTARQALRQRFLQADMGISGVNFGVADSGAIVIVENEGNARLSTTVPRIHVAIMGIERI
VARLADLGVMLQVLARSATGQKLSVYTSLISGPARPGEEDGPEEFHLVLLDNGRSRILGGHYAESLLCIRCGACLNACPV
YQAIGGHAYGGVYSGPIGAILTPLLQPDLPDAYLLPQASSLCGACQEVCPVRIAIPDMLLRHRADAVKAGKANPIEKAAI
SGYTLTMTHPALYRASGKAARFFSRILARKGRFRRLPPPLHLWTQQRDFPAPPPKTFRELWAERQKQRRNEEQA

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=468, Percent_Identity=41.2393162393162, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 51818; Mature: 51687

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQTIAFYDRVDHALHDESLQTALTRATTRFVGNRAGAIGALSDADRLRDQARAIRAHAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AHLDELLPQLAANVEARGGHVCWAADGEEARRYIIDLARARGVKSIVKSKSMASEEIHLN
HHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALEHAGFEVVETDLGEYIIQLAGETPSHIIAPAIHKTREQVSELFAQHLGMQPTTEVPP
HHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
MIRTARQALRQRFLQADMGISGVNFGVADSGAIVIVENEGNARLSTTVPRIHVAIMGIER
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHH
IVARLADLGVMLQVLARSATGQKLSVYTSLISGPARPGEEDGPEEFHLVLLDNGRSRILG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCC
GHYAESLLCIRCGACLNACPVYQAIGGHAYGGVYSGPIGAILTPLLQPDLPDAYLLPQAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCHH
SLCGACQEVCPVRIAIPDMLLRHRADAVKAGKANPIEKAAISGYTLTMTHPALYRASGKA
HHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCHHHHCCCHH
ARFFSRILARKGRFRRLPPPLHLWTQQRDFPAPPPKTFRELWAERQKQRRNEEQA
HHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AQTIAFYDRVDHALHDESLQTALTRATTRFVGNRAGAIGALSDADRLRDQARAIRAHAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
AHLDELLPQLAANVEARGGHVCWAADGEEARRYIIDLARARGVKSIVKSKSMASEEIHLN
HHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EALEHAGFEVVETDLGEYIIQLAGETPSHIIAPAIHKTREQVSELFAQHLGMQPTTEVPP
HHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
MIRTARQALRQRFLQADMGISGVNFGVADSGAIVIVENEGNARLSTTVPRIHVAIMGIER
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHHHHHHHH
IVARLADLGVMLQVLARSATGQKLSVYTSLISGPARPGEEDGPEEFHLVLLDNGRSRILG
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCC
GHYAESLLCIRCGACLNACPVYQAIGGHAYGGVYSGPIGAILTPLLQPDLPDAYLLPQAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCEECCCHH
SLCGACQEVCPVRIAIPDMLLRHRADAVKAGKANPIEKAAISGYTLTMTHPALYRASGKA
HHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCHHHHCCCHH
ARFFSRILARKGRFRRLPPPLHLWTQQRDFPAPPPKTFRELWAERQKQRRNEEQA
HHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]