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The map label for this gene is nikA [C]

Identifier: 222524140

GI number: 222524140

Start: 1080624

End: 1082462

Strand: Reverse

Name: nikA [C]

Synonym: Chy400_0856

Alternate gene names: 222524140

Gene position: 1082462-1080624 (Counterclockwise)

Preceding gene: 222524141

Following gene: 222524139

Centisome position: 20.54

GC content: 57.8

Gene sequence:

>1839_bases
GTGTTGTCCCACATGGTTTCTCGGCTTTTCCTGATCATGATCGTTTTGCTCCTGGCGGCATGTGGTCAGCAAACGTCACA
ATCGCAGCCGCCAACTCTACCGTCGGCACAGGAAACAGTAACAGCAGGGCCAACTGTAGCGCCGCAACCAACGGCGGTGC
CAGAGGCAACGGCGGTACCGGCATCTACCGCCGTGCCGACAGCGATCCCGCCATCGGGAGGGAAGCTCACCATTCTCTCC
TGGCAGGCAATAACGACGTTGAACCCGCATCTGGCTACCGGCGTCAAAGATTTCAACGGCGCCACCGTGATTCTTGAACC
ACTGGCACGCTACAACGAGCGTGACGAACTGGTGCCGTTCCTGGCCGCCGAAATCCCAACCCTTGCCAACGGTGGCATTG
CTGCCGACGGTACCAGCATCACCTGGAAGATCAAACCGGGGTTGAAATGGTCAGACGGGAGCGATTTCACGATTGACGAT
ATTATCTTCACCTGGCGCTATTGTGTTGACGAAGCGACCGGCTGTACGAATAAGACCTCATTCGATCCGATTGCCGACAT
CACGCCAATCGATGATACAACGTTCACCATCACCTGGAAGGAACCGAACCCCAACCCCTACATCGCCTTTGTCGGATCGG
CAGGCATGATCCTGCAACAAAAGCAATTTGCAAATTGTATCGGGGCTGCGGCAAACACAAGTGCCGAGTGCCAGGCCGCC
AACCTGGCTCCGATTGGTACCAATGCCTGGAAAGTGAAAGAGTTCCGTCCAGGCGACACCGTCATCTACGAACGCAACCC
CTTCTACCGCGATGCCGACAACGTCTTCTTCGACGAGGTTGAGATCAAGGGTGGTGGTGATGCCGCCTCGGCGGCGCGTG
CCGTCTGCACAACAGGGGAAGCCGATTTTGCCTTGAATATGCAGGTTCCCAAAGCGGTACTGGAGCCGATTATTGCCGGT
GGCGCCTGTGATGTGGTGGCCGGTGGTTCAACCGGCGGTGTTGAGCGTATCGTGATCAACTTCACCAACCCCGATCCGGC
CCTCGGCGACCGGCGCAGCGAGCCTGATCAGCCGCATCCCTTCCTGACCGATCCGGCAGTACGCCGGGCAATTGCGCTGG
CAATTGATCGCAAGGCAATTGCCGACCAGCTCTACGGGCCGACGGGTGCGCCGACCTGCAATCTGCTGGTGGTACCGGCC
CAGATGAATTCACCCAATACAACCTGCGAGCGTAATGTGGCCGAGGCAAAGCGGCTGCTGGACGAAGCCGGTTGGGTGCT
GAAGGATGGAGTGCGAGAGAAAGATGGCGTAAAGCTGGTCGTTACCCTACAGACCAGCGTGAATAACCTGCGCCAGAGCA
CGCAGGCCATCATCAAAGCCAACCTGGCCGAGATCGGCATTCAGACCTACCTCAAGGCGATTGATCCGGCTGTCTTCTTC
AGCGGCGATCCGGGCAACCCCGATACGCTGAACAAGTTCTACGCCGACCTGCAAATGTACACCTTCGGTCCCAGCTCCCC
CGATCCACAGTCATATCTGGAAGGCTGGATCTGCGCCGAGGTAACGTCGGCGGCGAACCGCTGGAACGGCAACAACGTGA
GCCGCTACTGCAACCCGACCTACGATGCGCTGTTTGCACAGTTGAAGACCACCTTTGATCCGGCGCAGCGGGCGCAGTTG
GCAATTCAGATGAACGATCTGTTGGTCAACGACGTGGTCGAGATACCGCTGATCAACCGTTACACACCGGTAGTCAAGCT
CAAGAATCTGGACGGGCCGACCTTCAATACCTTTGACAGCAATCTCTGGAATATTGCTAGCTGGCGGCGCGTGCCGTAA

Upstream 100 bases:

>100_bases
TTCGATCATCTGGTCTGTCGATGATTGATGAAGGTGACTGCTAACTATCAGTTTTATGACACCTTACCCGAATCGTGGTG
AGGTGTAGAGGGAGGTTTCT

Downstream 100 bases:

>100_bases
GGTTTGGCGAACGGGCAATAACGGACACTGGTGCGCGTCCCGCAACGCAAGGCGACACAGGGTGCGAATATCAACCGGAG
ACACCAGGTAGGGGCGGGTT

Product: family 5 extracellular solute-binding protein

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: 4-Phytase; Peptide ABC Transporter Periplasmic Protein; ABC-Type Dipeptide Transport System Periplasmic Component; Twin-Arginine Translocation Pathway Signal; Peptide Transport Periplasmic Protein SapA; ABC-Type Dipeptide Transport System; ABC Transporter; Dipeptide ABC Transporter Periplasmic Component; AppA Protein; Peptide Transport Periplasmic Protein SapA; Peptide Transport Protein SapA

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MLSHMVSRLFLIMIVLLLAACGQQTSQSQPPTLPSAQETVTAGPTVAPQPTAVPEATAVPASTAVPTAIPPSGGKLTILS
WQAITTLNPHLATGVKDFNGATVILEPLARYNERDELVPFLAAEIPTLANGGIAADGTSITWKIKPGLKWSDGSDFTIDD
IIFTWRYCVDEATGCTNKTSFDPIADITPIDDTTFTITWKEPNPNPYIAFVGSAGMILQQKQFANCIGAAANTSAECQAA
NLAPIGTNAWKVKEFRPGDTVIYERNPFYRDADNVFFDEVEIKGGGDAASAARAVCTTGEADFALNMQVPKAVLEPIIAG
GACDVVAGGSTGGVERIVINFTNPDPALGDRRSEPDQPHPFLTDPAVRRAIALAIDRKAIADQLYGPTGAPTCNLLVVPA
QMNSPNTTCERNVAEAKRLLDEAGWVLKDGVREKDGVKLVVTLQTSVNNLRQSTQAIIKANLAEIGIQTYLKAIDPAVFF
SGDPGNPDTLNKFYADLQMYTFGPSSPDPQSYLEGWICAEVTSAANRWNGNNVSRYCNPTYDALFAQLKTTFDPAQRAQL
AIQMNDLLVNDVVEIPLINRYTPVVKLKNLDGPTFNTFDSNLWNIASWRRVP

Sequences:

>Translated_612_residues
MLSHMVSRLFLIMIVLLLAACGQQTSQSQPPTLPSAQETVTAGPTVAPQPTAVPEATAVPASTAVPTAIPPSGGKLTILS
WQAITTLNPHLATGVKDFNGATVILEPLARYNERDELVPFLAAEIPTLANGGIAADGTSITWKIKPGLKWSDGSDFTIDD
IIFTWRYCVDEATGCTNKTSFDPIADITPIDDTTFTITWKEPNPNPYIAFVGSAGMILQQKQFANCIGAAANTSAECQAA
NLAPIGTNAWKVKEFRPGDTVIYERNPFYRDADNVFFDEVEIKGGGDAASAARAVCTTGEADFALNMQVPKAVLEPIIAG
GACDVVAGGSTGGVERIVINFTNPDPALGDRRSEPDQPHPFLTDPAVRRAIALAIDRKAIADQLYGPTGAPTCNLLVVPA
QMNSPNTTCERNVAEAKRLLDEAGWVLKDGVREKDGVKLVVTLQTSVNNLRQSTQAIIKANLAEIGIQTYLKAIDPAVFF
SGDPGNPDTLNKFYADLQMYTFGPSSPDPQSYLEGWICAEVTSAANRWNGNNVSRYCNPTYDALFAQLKTTFDPAQRAQL
AIQMNDLLVNDVVEIPLINRYTPVVKLKNLDGPTFNTFDSNLWNIASWRRVP
>Mature_612_residues
MLSHMVSRLFLIMIVLLLAACGQQTSQSQPPTLPSAQETVTAGPTVAPQPTAVPEATAVPASTAVPTAIPPSGGKLTILS
WQAITTLNPHLATGVKDFNGATVILEPLARYNERDELVPFLAAEIPTLANGGIAADGTSITWKIKPGLKWSDGSDFTIDD
IIFTWRYCVDEATGCTNKTSFDPIADITPIDDTTFTITWKEPNPNPYIAFVGSAGMILQQKQFANCIGAAANTSAECQAA
NLAPIGTNAWKVKEFRPGDTVIYERNPFYRDADNVFFDEVEIKGGGDAASAARAVCTTGEADFALNMQVPKAVLEPIIAG
GACDVVAGGSTGGVERIVINFTNPDPALGDRRSEPDQPHPFLTDPAVRRAIALAIDRKAIADQLYGPTGAPTCNLLVVPA
QMNSPNTTCERNVAEAKRLLDEAGWVLKDGVREKDGVKLVVTLQTSVNNLRQSTQAIIKANLAEIGIQTYLKAIDPAVFF
SGDPGNPDTLNKFYADLQMYTFGPSSPDPQSYLEGWICAEVTSAANRWNGNNVSRYCNPTYDALFAQLKTTFDPAQRAQL
AIQMNDLLVNDVVEIPLINRYTPVVKLKNLDGPTFNTFDSNLWNIASWRRVP

Specific function: Involved In A Nickel Transport System, Probably Represents The Nickel Binder. [C]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 65919; Mature: 65919

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHMVSRLFLIMIVLLLAACGQQTSQSQPPTLPSAQETVTAGPTVAPQPTAVPEATAVP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
ASTAVPTAIPPSGGKLTILSWQAITTLNPHLATGVKDFNGATVILEPLARYNERDELVPF
CCCCCCCCCCCCCCEEEEEEEEEEEECCCHHHCCCCCCCCCEEEEEHHHCCCCCHHHHHH
LAAEIPTLANGGIAADGTSITWKIKPGLKWSDGSDFTIDDIIFTWRYCVDEATGCTNKTS
HHHCCCCCCCCCEEECCCEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
FDPIADITPIDDTTFTITWKEPNPNPYIAFVGSAGMILQQKQFANCIGAAANTSAECQAA
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCEEC
NLAPIGTNAWKVKEFRPGDTVIYERNPFYRDADNVFFDEVEIKGGGDAASAARAVCTTGE
CCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHEECCC
ADFALNMQVPKAVLEPIIAGGACDVVAGGSTGGVERIVINFTNPDPALGDRRSEPDQPHP
CCEEEECCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
FLTDPAVRRAIALAIDRKAIADQLYGPTGAPTCNLLVVPAQMNSPNTTCERNVAEAKRLL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
DEAGWVLKDGVREKDGVKLVVTLQTSVNNLRQSTQAIIKANLAEIGIQTYLKAIDPAVFF
HHCCCHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SGDPGNPDTLNKFYADLQMYTFGPSSPDPQSYLEGWICAEVTSAANRWNGNNVSRYCNPT
CCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCH
YDALFAQLKTTFDPAQRAQLAIQMNDLLVNDVVEIPLINRYTPVVKLKNLDGPTFNTFDS
HHHHHHHHHHCCCHHHHHEEEEEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCC
NLWNIASWRRVP
CCCCCCCCCCCC
>Mature Secondary Structure
MLSHMVSRLFLIMIVLLLAACGQQTSQSQPPTLPSAQETVTAGPTVAPQPTAVPEATAVP
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCC
ASTAVPTAIPPSGGKLTILSWQAITTLNPHLATGVKDFNGATVILEPLARYNERDELVPF
CCCCCCCCCCCCCCEEEEEEEEEEEECCCHHHCCCCCCCCCEEEEEHHHCCCCCHHHHHH
LAAEIPTLANGGIAADGTSITWKIKPGLKWSDGSDFTIDDIIFTWRYCVDEATGCTNKTS
HHHCCCCCCCCCEEECCCEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCC
FDPIADITPIDDTTFTITWKEPNPNPYIAFVGSAGMILQQKQFANCIGAAANTSAECQAA
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCEEC
NLAPIGTNAWKVKEFRPGDTVIYERNPFYRDADNVFFDEVEIKGGGDAASAARAVCTTGE
CCCCCCCCCEEEEEECCCCEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHEECCC
ADFALNMQVPKAVLEPIIAGGACDVVAGGSTGGVERIVINFTNPDPALGDRRSEPDQPHP
CCEEEECCCCHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
FLTDPAVRRAIALAIDRKAIADQLYGPTGAPTCNLLVVPAQMNSPNTTCERNVAEAKRLL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHH
DEAGWVLKDGVREKDGVKLVVTLQTSVNNLRQSTQAIIKANLAEIGIQTYLKAIDPAVFF
HHCCCHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
SGDPGNPDTLNKFYADLQMYTFGPSSPDPQSYLEGWICAEVTSAANRWNGNNVSRYCNPT
CCCCCCCHHHHHHHHCEEEEEECCCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCH
YDALFAQLKTTFDPAQRAQLAIQMNDLLVNDVVEIPLINRYTPVVKLKNLDGPTFNTFDS
HHHHHHHHHHCCCHHHHHEEEEEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCC
NLWNIASWRRVP
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA