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The map label for this gene is nth [H]

Identifier: 222524128

GI number: 222524128

Start: 1062725

End: 1063387

Strand: Reverse

Name: nth [H]

Synonym: Chy400_0844

Alternate gene names: 222524128

Gene position: 1063387-1062725 (Counterclockwise)

Preceding gene: 222524131

Following gene: 222524126

Centisome position: 20.18

GC content: 58.82

Gene sequence:

>663_bases
TTGAGTACGTCGTCGTTTCCGATTGAACAGGTGCTCGATACGCTGGAGCGTGAGCTGGCGGTGTATACGCCGCCGCTGAT
TGACCAGATGGGAGAGGTGAGTCAGACGCCGTTTCGGATTCTGATTGCGACGATCCTCAGTTTGCGAACGAAAGATACGT
TGACGGCGGTGGTTGCGCCGCGGCTGTTTGCGGTGGCCGATACGCCGGCGGCGATGGTGGCGTTGGGGGCGGAACGGATT
GCTGAGTTGATCTATCCGGTTGGGTTTTATCGGGTGAAGGCGCAGCAGATTGTGCATATCTGTCAGATATTGCTCGAACG
GTATAACGGTGAGGTGCCCGCCGATCTGGACGAGTTGCTGAAGCTGCCCGGTGTCGGGCGTAAGACGGCTAATCTGGTGG
TGACGGCGGGCTTTGGGCTGCCGGGGATTTGTGTTGATATTCACGTCCATCGCATTTGCAACCGCTGGGGTTATGTGCAG
ACGCGCACGCCGGAAGAGACGGAGATGGCGTTGCGGGCGAGGTTGCCGCAACGCTATTGGATACCGATTAATCGCTTGCT
GGTGACGCTGGGGCAGAATATCTGTCACCCTACGTCGCCGCGGTGTAGTATCTGCCCGATCCGTGAGGTCTGTCCGCGGA
TTGGGGTGACGCGCAGTCGGTGA

Upstream 100 bases:

>100_bases
GGCCCGGCAGTAGTGGTCATAATTGCCGACTTCGCGTATCATCATACCGTATGCAGGGCTGCTTGAACAGGGCTGGCCGG
TCTCTGGCAGAGGAGGTTGC

Downstream 100 bases:

>100_bases
TGGTGGGGCGTTTACGATGGGTTCGAGTGTACCGGGGCCAGGAAGCCATTTAGCAGGCCACGAAACGGGCGCACGGCTTC
GATGTGCGGGAAGTGGCCGC

Product: DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MSTSSFPIEQVLDTLERELAVYTPPLIDQMGEVSQTPFRILIATILSLRTKDTLTAVVAPRLFAVADTPAAMVALGAERI
AELIYPVGFYRVKAQQIVHICQILLERYNGEVPADLDELLKLPGVGRKTANLVVTAGFGLPGICVDIHVHRICNRWGYVQ
TRTPEETEMALRARLPQRYWIPINRLLVTLGQNICHPTSPRCSICPIREVCPRIGVTRSR

Sequences:

>Translated_220_residues
MSTSSFPIEQVLDTLERELAVYTPPLIDQMGEVSQTPFRILIATILSLRTKDTLTAVVAPRLFAVADTPAAMVALGAERI
AELIYPVGFYRVKAQQIVHICQILLERYNGEVPADLDELLKLPGVGRKTANLVVTAGFGLPGICVDIHVHRICNRWGYVQ
TRTPEETEMALRARLPQRYWIPINRLLVTLGQNICHPTSPRCSICPIREVCPRIGVTRSR
>Mature_219_residues
STSSFPIEQVLDTLERELAVYTPPLIDQMGEVSQTPFRILIATILSLRTKDTLTAVVAPRLFAVADTPAAMVALGAERIA
ELIYPVGFYRVKAQQIVHICQILLERYNGEVPADLDELLKLPGVGRKTANLVVTAGFGLPGICVDIHVHRICNRWGYVQT
RTPEETEMALRARLPQRYWIPINRLLVTLGQNICHPTSPRCSICPIREVCPRIGVTRSR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=178, Percent_Identity=42.6966292134831, Blast_Score=144, Evalue=6e-35,
Organism=Escherichia coli, GI1787920, Length=177, Percent_Identity=40.6779661016949, Blast_Score=148, Evalue=2e-37,
Organism=Escherichia coli, GI1789331, Length=217, Percent_Identity=23.963133640553, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=176, Percent_Identity=41.4772727272727, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=35.8974358974359, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319304, Length=192, Percent_Identity=34.375, Blast_Score=112, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=37.0786516853933, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24553; Mature: 24422

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSFPIEQVLDTLERELAVYTPPLIDQMGEVSQTPFRILIATILSLRTKDTLTAVVAP
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLFAVADTPAAMVALGAERIAELIYPVGFYRVKAQQIVHICQILLERYNGEVPADLDELL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KLPGVGRKTANLVVTAGFGLPGICVDIHVHRICNRWGYVQTRTPEETEMALRARLPQRYW
HCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
IPINRLLVTLGQNICHPTSPRCSICPIREVCPRIGVTRSR
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
STSSFPIEQVLDTLERELAVYTPPLIDQMGEVSQTPFRILIATILSLRTKDTLTAVVAP
CCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RLFAVADTPAAMVALGAERIAELIYPVGFYRVKAQQIVHICQILLERYNGEVPADLDELL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
KLPGVGRKTANLVVTAGFGLPGICVDIHVHRICNRWGYVQTRTPEETEMALRARLPQRYW
HCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCC
IPINRLLVTLGQNICHPTSPRCSICPIREVCPRIGVTRSR
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]