Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 222524111

GI number: 222524111

Start: 1040495

End: 1041400

Strand: Reverse

Name: mraW

Synonym: Chy400_0827

Alternate gene names: 222524111

Gene position: 1041400-1040495 (Counterclockwise)

Preceding gene: 222524112

Following gene: 222524110

Centisome position: 19.76

GC content: 58.94

Gene sequence:

>906_bases
ATGGCGGTAACGTTCCAGCATACACCGGTTCTATTAACCGAGGTGCTGACGATGCTGGCACCGCGCCCCGCCGGGCAGTA
TCTCGATGCAACTGTCGGTGGTGGGGGGCACGCGCTGGCGGTGTTGCAGGCAGCGCAGCCCGGTGGCAGGTTGCTGGGGA
TCGACGCCGATCCCGCTGCACTTGCGGCAACTGCTGCTCGCTTGCAGGCAGCCGGTCTGATCGAACAGGCCGTGCTCTGC
CATGGCTCTTTTGCCGATCTAGCAACGCTTGCCGCTACTGCCGGTTTTGGCGCTTTTGATGGCATTCTGTTCGATCTGGG
GGTGTCGTCATACCAGCTTGATACGCCTGAACGCGGCTTTTCGTTCACGGCAGATGGCCCTCTCGATATGAGGCTTGATC
CAACGCAGGGCTTGACCGCTGCCGATATGGTGAACAGATTGAGCGAACGTGAACTGGCCGACATTATCTTCTTGTATGGT
GAAGAGCATGCTGCCCGCCGCATTGCACGGGCAATCGTTGAGCATCGGCGAACTCAACCCTTTCAACGCACGGCAGAACT
GGCCGAGGTCGTTGCGCGTGCAGTCGGTGGGCGTCATGGTCGTATTCATCCGGCAACCCGCACGTTTCAAGCCCTGCGGA
TTGCGGTAAACCAGGAGCTTGATCGGTTGCGGGCTACTCTACCCCAGGCGGTCGATCTATTGGCACCGGGTGGTCGATTG
GCGGTGATCAGTTTTCATTCGCTGGAAGATCGTATCGTGAAGCAGTTTCTGCGGGCCGAAGCATCTGGAGAGACACCACG
GCTAACAATTGTAACGAAGAAGCCGATAGTGCCGACGGCTGCGGAAGTGGCAAACAATCCACGTGCGCGCAGTGCAAAGC
TACGGGTTGCTACCCGGATCGGATAG

Upstream 100 bases:

>100_bases
TTATTGAAGTTTGGGCACCTGAGCGCTGGCGTGAGGTGCAACAACGTCTGGAAAGTCAGGGGCCACACTTCGATGAACAG
ATGCGGAAGTTGGGGATTTG

Downstream 100 bases:

>100_bases
CGGGTCATTGAGAGGATAGAGGTATGGCAGTACGAACTGAACACATTCCAGCCATTGTGGGCCGAGTGCGGTTGCGACGG
GTTGACCTTTCGCGCTACCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAVTFQHTPVLLTEVLTMLAPRPAGQYLDATVGGGGHALAVLQAAQPGGRLLGIDADPAALAATAARLQAAGLIEQAVLC
HGSFADLATLAATAGFGAFDGILFDLGVSSYQLDTPERGFSFTADGPLDMRLDPTQGLTAADMVNRLSERELADIIFLYG
EEHAARRIARAIVEHRRTQPFQRTAELAEVVARAVGGRHGRIHPATRTFQALRIAVNQELDRLRATLPQAVDLLAPGGRL
AVISFHSLEDRIVKQFLRAEASGETPRLTIVTKKPIVPTAAEVANNPRARSAKLRVATRIG

Sequences:

>Translated_301_residues
MAVTFQHTPVLLTEVLTMLAPRPAGQYLDATVGGGGHALAVLQAAQPGGRLLGIDADPAALAATAARLQAAGLIEQAVLC
HGSFADLATLAATAGFGAFDGILFDLGVSSYQLDTPERGFSFTADGPLDMRLDPTQGLTAADMVNRLSERELADIIFLYG
EEHAARRIARAIVEHRRTQPFQRTAELAEVVARAVGGRHGRIHPATRTFQALRIAVNQELDRLRATLPQAVDLLAPGGRL
AVISFHSLEDRIVKQFLRAEASGETPRLTIVTKKPIVPTAAEVANNPRARSAKLRVATRIG
>Mature_300_residues
AVTFQHTPVLLTEVLTMLAPRPAGQYLDATVGGGGHALAVLQAAQPGGRLLGIDADPAALAATAARLQAAGLIEQAVLCH
GSFADLATLAATAGFGAFDGILFDLGVSSYQLDTPERGFSFTADGPLDMRLDPTQGLTAADMVNRLSERELADIIFLYGE
EHAARRIARAIVEHRRTQPFQRTAELAEVVARAVGGRHGRIHPATRTFQALRIAVNQELDRLRATLPQAVDLLAPGGRLA
VISFHSLEDRIVKQFLRAEASGETPRLTIVTKKPIVPTAAEVANNPRARSAKLRVATRIG

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=41.4705882352941, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=45.3125, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1786270, Length=317, Percent_Identity=47.6340694006309, Blast_Score=261, Evalue=3e-71,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=37.3860182370821, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=37.3860182370821, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_CHLAA (A9WG80)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001634390.1
- ProteinModelPortal:   A9WG80
- SMR:   A9WG80
- GeneID:   5827858
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0763
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 32052; Mature: 31921

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 84-84 BINDING 105-105 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTFQHTPVLLTEVLTMLAPRPAGQYLDATVGGGGHALAVLQAAQPGGRLLGIDADPAA
CEEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEECCCCHHH
LAATAARLQAAGLIEQAVLCHGSFADLATLAATAGFGAFDGILFDLGVSSYQLDTPERGF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCCCC
SFTADGPLDMRLDPTQGLTAADMVNRLSERELADIIFLYGEEHAARRIARAIVEHRRTQP
EECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCH
FQRTAELAEVVARAVGGRHGRIHPATRTFQALRIAVNQELDRLRATLPQAVDLLAPGGRL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AVISFHSLEDRIVKQFLRAEASGETPRLTIVTKKPIVPTAAEVANNPRARSAKLRVATRI
EEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCEEEEEECC
G
C
>Mature Secondary Structure 
AVTFQHTPVLLTEVLTMLAPRPAGQYLDATVGGGGHALAVLQAAQPGGRLLGIDADPAA
EEEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCEEEECCCCHHH
LAATAARLQAAGLIEQAVLCHGSFADLATLAATAGFGAFDGILFDLGVSSYQLDTPERGF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCCCC
SFTADGPLDMRLDPTQGLTAADMVNRLSERELADIIFLYGEEHAARRIARAIVEHRRTQP
EECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHCCCH
FQRTAELAEVVARAVGGRHGRIHPATRTFQALRIAVNQELDRLRATLPQAVDLLAPGGRL
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
AVISFHSLEDRIVKQFLRAEASGETPRLTIVTKKPIVPTAAEVANNPRARSAKLRVATRI
EEEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHCCCCCCCCCEEEEEECC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA