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The map label for this gene is prfB [H]

Identifier: 222524073

GI number: 222524073

Start: 995719

End: 996660

Strand: Reverse

Name: prfB [H]

Synonym: Chy400_0789

Alternate gene names: 222524073

Gene position: 996660-995719 (Counterclockwise)

Preceding gene: 222524075

Following gene: 222524072

Centisome position: 18.92

GC content: 58.49

Gene sequence:

>942_bases
ATGCAACGGCTCACCCGCCTGAAAGAAGAGGTGACGGCGTGGAATGATCTTGAACGGCGCATCGCCAGTGCAGCCGAACT
TATCGAACTGGCCGAGCTGGAAGGTGATGAGTCGTTGACCGCCGATCTGGCCGCTGAAGTACAGGCCATTCAGCGTGAAG
TGGCGCAGCGTGAGCTGGAAATCCTGCTTAGTGGCCCTTACGACGACCGTGATGCCTTTCTTTCGGTGCAGGCCGGCATG
GGTGGGACAGACGCACAAGACTGGGCGGCGATGCTTTTGCGCATGTACACGCGCTGGGCCGAACGGCGAGGGTATACGGT
CAACCTGATCGACATGAGCGAAGGCGAAGAGGCAGGAATCAAGAGTGCGACTATCGAGATTCGTGGCCCTTACGCTTACG
GCTACGCCCGCGCCGAGGCCGGTGTCCATCGCCTGATTCGCCTGTCGCCGTTCAACGCTGCGCACACCCGCCAGACCAGC
TTTGCGCGCGTCGAGGTGATGCCAGAAGTTGATGATGCGCCTGAAGTAGAGATCAAACCGGAAGACCTGCGCATTGATGT
CTTCCGCAGTGGGGGTCATGGCGGGCAAGGCGTGAATACCACCGATTCGGCGGTACGCATTACCCACCTGCCAACCGGAA
TTGTGGTTACCTGTCAGAATGAACGTTCGCAGATACAGAACCGCGAACTGGCCATGCGCGTGTTACGGGCGCGACTGCTC
GAACGCGAATTGCAGCGTCAGGCCGAAGAACGTGCGCGACTCCGGGGTGAATATCGCGAGGCCGCATTTGGTAACCAGAT
GCGAACCTATTACCTCCATCCTTCAACGCTGGTCAAAGATCATCGCACCGATTACGAAACGAGCAACGTACAGGCAGTGC
TCGATGGCGAGATCGATCCGTTTATTGAAGCCTTCCTACGGGCAAACGTGCGGGAGACGTGA

Upstream 100 bases:

>100_bases
CATCTTTGACCTGGCGGCAAAGCAGGCTGAAATTGAGCAGTTAGAAGCCAGGGCCGCCGATCCCGATCTATGGAATACAC
CGCGCGTTGCGCAGGAGATC

Downstream 100 bases:

>100_bases
TGGAAATCAAATATACGCCGTGGCGCATGCGCTATATCAAGCGGGGTGAGACGCCGGATGAGGGATGCGTGTTCTGTGCA
ATGGCAACTGCCGACGGCAC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MQRLTRLKEEVTAWNDLERRIASAAELIELAELEGDESLTADLAAEVQAIQREVAQRELEILLSGPYDDRDAFLSVQAGM
GGTDAQDWAAMLLRMYTRWAERRGYTVNLIDMSEGEEAGIKSATIEIRGPYAYGYARAEAGVHRLIRLSPFNAAHTRQTS
FARVEVMPEVDDAPEVEIKPEDLRIDVFRSGGHGGQGVNTTDSAVRITHLPTGIVVTCQNERSQIQNRELAMRVLRARLL
ERELQRQAEERARLRGEYREAAFGNQMRTYYLHPSTLVKDHRTDYETSNVQAVLDGEIDPFIEAFLRANVRET

Sequences:

>Translated_313_residues
MQRLTRLKEEVTAWNDLERRIASAAELIELAELEGDESLTADLAAEVQAIQREVAQRELEILLSGPYDDRDAFLSVQAGM
GGTDAQDWAAMLLRMYTRWAERRGYTVNLIDMSEGEEAGIKSATIEIRGPYAYGYARAEAGVHRLIRLSPFNAAHTRQTS
FARVEVMPEVDDAPEVEIKPEDLRIDVFRSGGHGGQGVNTTDSAVRITHLPTGIVVTCQNERSQIQNRELAMRVLRARLL
ERELQRQAEERARLRGEYREAAFGNQMRTYYLHPSTLVKDHRTDYETSNVQAVLDGEIDPFIEAFLRANVRET
>Mature_313_residues
MQRLTRLKEEVTAWNDLERRIASAAELIELAELEGDESLTADLAAEVQAIQREVAQRELEILLSGPYDDRDAFLSVQAGM
GGTDAQDWAAMLLRMYTRWAERRGYTVNLIDMSEGEEAGIKSATIEIRGPYAYGYARAEAGVHRLIRLSPFNAAHTRQTS
FARVEVMPEVDDAPEVEIKPEDLRIDVFRSGGHGGQGVNTTDSAVRITHLPTGIVVTCQNERSQIQNRELAMRVLRARLL
ERELQRQAEERARLRGEYREAAFGNQMRTYYLHPSTLVKDHRTDYETSNVQAVLDGEIDPFIEAFLRANVRET

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=302, Percent_Identity=32.7814569536424, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI34577120, Length=290, Percent_Identity=34.4827586206897, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI166795305, Length=171, Percent_Identity=36.8421052631579, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI2367172, Length=306, Percent_Identity=49.3464052287582, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=44.4444444444444, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=303, Percent_Identity=28.3828382838284, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=315, Percent_Identity=33.6507936507937, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=323, Percent_Identity=34.3653250773994, Blast_Score=134, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 35435; Mature: 35435

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRLTRLKEEVTAWNDLERRIASAAELIELAELEGDESLTADLAAEVQAIQREVAQRELE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ILLSGPYDDRDAFLSVQAGMGGTDAQDWAAMLLRMYTRWAERRGYTVNLIDMSEGEEAGI
EEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
KSATIEIRGPYAYGYARAEAGVHRLIRLSPFNAAHTRQTSFARVEVMPEVDDAPEVEIKP
CEEEEEEECCEEECHHHHHHHHHHHHEECCCCCHHCCCCCEEEEEECCCCCCCCCCEECC
EDLRIDVFRSGGHGGQGVNTTDSAVRITHLPTGIVVTCQNERSQIQNRELAMRVLRARLL
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ERELQRQAEERARLRGEYREAAFGNQMRTYYLHPSTLVKDHRTDYETSNVQAVLDGEIDP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCH
FIEAFLRANVRET
HHHHHHHCCCCCC
>Mature Secondary Structure
MQRLTRLKEEVTAWNDLERRIASAAELIELAELEGDESLTADLAAEVQAIQREVAQRELE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ILLSGPYDDRDAFLSVQAGMGGTDAQDWAAMLLRMYTRWAERRGYTVNLIDMSEGEEAGI
EEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
KSATIEIRGPYAYGYARAEAGVHRLIRLSPFNAAHTRQTSFARVEVMPEVDDAPEVEIKP
CEEEEEEECCEEECHHHHHHHHHHHHEECCCCCHHCCCCCEEEEEECCCCCCCCCCEECC
EDLRIDVFRSGGHGGQGVNTTDSAVRITHLPTGIVVTCQNERSQIQNRELAMRVLRARLL
CCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHH
ERELQRQAEERARLRGEYREAAFGNQMRTYYLHPSTLVKDHRTDYETSNVQAVLDGEIDP
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCCCCCCCEEEEECCCCCH
FIEAFLRANVRET
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA