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The map label for this gene is 222523928

Identifier: 222523928

GI number: 222523928

Start: 786090

End: 787511

Strand: Reverse

Name: 222523928

Synonym: Chy400_0639

Alternate gene names: NA

Gene position: 787511-786090 (Counterclockwise)

Preceding gene: 222523933

Following gene: 222523927

Centisome position: 14.95

GC content: 57.52

Gene sequence:

>1422_bases
ATGACTGATACCGGGCTATGTCTCCAGCCGGCTACCACTATTGCGCACCTGATTCGACAACGCGCTGTTTCGGCGACTGA
AGTGCTGGTTGCCCATCTCGAACGCATCGCCACGCTGAATCCGCTGGTCAACGCTATCGTGACCCTCGACATTGATGGTG
CCCAGGCTCGTGCGCGAGCGGTTGATGAAGCTCTTGCCCGGGGTGATGATCCTGGTCCGTTAGCCGGTTTGCCGGTCGCT
CACAAGGACCTGGCCGAGACGAAGGGGATGCGAACAACCTATGGTTCTCCGATTTTTGCTGATTTCGTTCCCGATTTTGA
TGCGCTGATCGTTGCCCGGCTCAAAGCAGCCGGCGCGGTCACGGTTGGGAAGACGAATACCCCGGAATTTGGTGCCGGTT
CGCAAACCTTCAACCCTGTCTTTGGCCCTACCCGCAATCCCTACGATCTGAGTAAGACGTGTGGGGGGAGTAGTGGTGGG
GCTGCCGTTGCCCTGGCTTGTGGGATGATTGCGATTGCCGACGGCAGCGATCTGGGCGGATCGTTACGCAACCCGGCCGG
CTACTGCAATGTCGTGGGCTTTCGTCCTTCGCCGGGAAGAGTGCCGGTCTGGCCTGACCCGACCCCTTTTCTACCGTTTG
CTATCGATGGCCCGATGGCACGCACGGTTGCCGATATTGCCCTGATCTTGCAGGCTATCGCCGGCCCCGACTTGCGAGCG
CCGCTCTCGATCAGTGAACCGCCCTCGCTCTTTGCCCAACCACTAGAGCGTGATTTGCGCGGTGTACGTATTGCCTGGAG
TCCTGATTTGGGTGGATTACCGGTTGATCTGCGGGTGGCTGAGGTTATCGCTGCCCAACGCGATGTCTTCACCCAGATCG
GCTGTATTGTTGAAGAGGCAACACCCGATCTCAGTGATGCTGACGAGGTCTTTCAAGTCATGCGCGCTTTCCGCTACGAA
CTGACCCTTGGCGAGTTACTGGATCGCGAACGTCACCGCATCAAAGACACTGTGGTCTGGAATATTGAAGCCGGACGCGC
CTTAAGCGGGCCGCAAGTTGGGCGAGCTATGCGTCTCCACGCTGCTCTCCTGGCCCGTGTGTATGAATTCATGCAAACCT
ATGAATTCATCATTGCCCCGGTGAGCCAGGTGCCACCGTTTCCGGTTGAACAACCGTACATTACCGAGATTAACGGGATA
CCAATGCAGAACTATATTGAATGGATGCGTTCGTGTTACTACATCTCTGTCTGCAACCTGCCGGCCATTTCGGTACCGGC
TGGCTTTACCCGCGATGGACTACCGGTTGGGATCCAAATCATCGGCCGTCCACGTGCCGATCTAAGCGTGCTCCAACTGG
CGTATGCCTTTGAACAGGCGACGCAACACTGGCAACGCCATCCCCAACTGGCAACAGGGTGA

Upstream 100 bases:

>100_bases
TCACCCATCCCGGCGGGTGCAGCGTTGCCGGCACGTGTTGTACGTCAGTCTGCTACAATTGCTGCGCAGCAACGCTTTTG
ATCAGGGAAGAGGTACACCT

Downstream 100 bases:

>100_bases
AATTGAGAGTGCTATAATGCAGATGTGCAAACGACATCAGCGCCAGTGAGAATACCACCTGTCTTGGGGCGGCCACAGAT
CATCTTCTTCTGTGAATTGG

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTDTGLCLQPATTIAHLIRQRAVSATEVLVAHLERIATLNPLVNAIVTLDIDGAQARARAVDEALARGDDPGPLAGLPVA
HKDLAETKGMRTTYGSPIFADFVPDFDALIVARLKAAGAVTVGKTNTPEFGAGSQTFNPVFGPTRNPYDLSKTCGGSSGG
AAVALACGMIAIADGSDLGGSLRNPAGYCNVVGFRPSPGRVPVWPDPTPFLPFAIDGPMARTVADIALILQAIAGPDLRA
PLSISEPPSLFAQPLERDLRGVRIAWSPDLGGLPVDLRVAEVIAAQRDVFTQIGCIVEEATPDLSDADEVFQVMRAFRYE
LTLGELLDRERHRIKDTVVWNIEAGRALSGPQVGRAMRLHAALLARVYEFMQTYEFIIAPVSQVPPFPVEQPYITEINGI
PMQNYIEWMRSCYYISVCNLPAISVPAGFTRDGLPVGIQIIGRPRADLSVLQLAYAFEQATQHWQRHPQLATG

Sequences:

>Translated_473_residues
MTDTGLCLQPATTIAHLIRQRAVSATEVLVAHLERIATLNPLVNAIVTLDIDGAQARARAVDEALARGDDPGPLAGLPVA
HKDLAETKGMRTTYGSPIFADFVPDFDALIVARLKAAGAVTVGKTNTPEFGAGSQTFNPVFGPTRNPYDLSKTCGGSSGG
AAVALACGMIAIADGSDLGGSLRNPAGYCNVVGFRPSPGRVPVWPDPTPFLPFAIDGPMARTVADIALILQAIAGPDLRA
PLSISEPPSLFAQPLERDLRGVRIAWSPDLGGLPVDLRVAEVIAAQRDVFTQIGCIVEEATPDLSDADEVFQVMRAFRYE
LTLGELLDRERHRIKDTVVWNIEAGRALSGPQVGRAMRLHAALLARVYEFMQTYEFIIAPVSQVPPFPVEQPYITEINGI
PMQNYIEWMRSCYYISVCNLPAISVPAGFTRDGLPVGIQIIGRPRADLSVLQLAYAFEQATQHWQRHPQLATG
>Mature_472_residues
TDTGLCLQPATTIAHLIRQRAVSATEVLVAHLERIATLNPLVNAIVTLDIDGAQARARAVDEALARGDDPGPLAGLPVAH
KDLAETKGMRTTYGSPIFADFVPDFDALIVARLKAAGAVTVGKTNTPEFGAGSQTFNPVFGPTRNPYDLSKTCGGSSGGA
AVALACGMIAIADGSDLGGSLRNPAGYCNVVGFRPSPGRVPVWPDPTPFLPFAIDGPMARTVADIALILQAIAGPDLRAP
LSISEPPSLFAQPLERDLRGVRIAWSPDLGGLPVDLRVAEVIAAQRDVFTQIGCIVEEATPDLSDADEVFQVMRAFRYEL
TLGELLDRERHRIKDTVVWNIEAGRALSGPQVGRAMRLHAALLARVYEFMQTYEFIIAPVSQVPPFPVEQPYITEINGIP
MQNYIEWMRSCYYISVCNLPAISVPAGFTRDGLPVGIQIIGRPRADLSVLQLAYAFEQATQHWQRHPQLATG

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=27.5100401606426, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI222831590, Length=499, Percent_Identity=26.4529058116232, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI166795287, Length=184, Percent_Identity=29.3478260869565, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=25.7425742574257, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17543272, Length=453, Percent_Identity=27.1523178807947, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=266, Percent_Identity=29.6992481203008, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556264, Length=393, Percent_Identity=25.1908396946565, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=198, Percent_Identity=30.3030303030303, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=323, Percent_Identity=29.7213622291022, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=323, Percent_Identity=29.7213622291022, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=263, Percent_Identity=26.2357414448669, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=398, Percent_Identity=26.6331658291457, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=175, Percent_Identity=36.5714285714286, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6320448, Length=243, Percent_Identity=29.2181069958848, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45550774, Length=512, Percent_Identity=26.3671875, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI24648435, Length=512, Percent_Identity=26.3671875, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI24648437, Length=512, Percent_Identity=26.3671875, Blast_Score=158, Evalue=9e-39,
Organism=Drosophila melanogaster, GI21356731, Length=493, Percent_Identity=23.5294117647059, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078093, Length=484, Percent_Identity=25.2066115702479, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652985, Length=504, Percent_Identity=27.7777777777778, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922090, Length=504, Percent_Identity=27.7777777777778, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652981, Length=504, Percent_Identity=27.7777777777778, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652983, Length=504, Percent_Identity=27.7777777777778, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24644968, Length=490, Percent_Identity=23.469387755102, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24648113, Length=453, Percent_Identity=28.6975717439294, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24648441, Length=442, Percent_Identity=24.8868778280543, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24648439, Length=442, Percent_Identity=24.8868778280543, Blast_Score=118, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 50861; Mature: 50730

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTGLCLQPATTIAHLIRQRAVSATEVLVAHLERIATLNPLVNAIVTLDIDGAQARARA
CCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHH
VDEALARGDDPGPLAGLPVAHKDLAETKGMRTTYGSPIFADFVPDFDALIVARLKAAGAV
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCEE
TVGKTNTPEFGAGSQTFNPVFGPTRNPYDLSKTCGGSSGGAAVALACGMIAIADGSDLGG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHCEEEEECCCCCCC
SLRNPAGYCNVVGFRPSPGRVPVWPDPTPFLPFAIDGPMARTVADIALILQAIAGPDLRA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCC
PLSISEPPSLFAQPLERDLRGVRIAWSPDLGGLPVDLRVAEVIAAQRDVFTQIGCIVEEA
CCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TPDLSDADEVFQVMRAFRYELTLGELLDRERHRIKDTVVWNIEAGRALSGPQVGRAMRLH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
AALLARVYEFMQTYEFIIAPVSQVPPFPVEQPYITEINGIPMQNYIEWMRSCYYISVCNL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCEEEEECCC
PAISVPAGFTRDGLPVGIQIIGRPRADLSVLQLAYAFEQATQHWQRHPQLATG
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDTGLCLQPATTIAHLIRQRAVSATEVLVAHLERIATLNPLVNAIVTLDIDGAQARARA
CCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHH
VDEALARGDDPGPLAGLPVAHKDLAETKGMRTTYGSPIFADFVPDFDALIVARLKAAGAV
HHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHCCEE
TVGKTNTPEFGAGSQTFNPVFGPTRNPYDLSKTCGGSSGGAAVALACGMIAIADGSDLGG
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHCEEEEECCCCCCC
SLRNPAGYCNVVGFRPSPGRVPVWPDPTPFLPFAIDGPMARTVADIALILQAIAGPDLRA
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCC
PLSISEPPSLFAQPLERDLRGVRIAWSPDLGGLPVDLRVAEVIAAQRDVFTQIGCIVEEA
CCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
TPDLSDADEVFQVMRAFRYELTLGELLDRERHRIKDTVVWNIEAGRALSGPQVGRAMRLH
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHH
AALLARVYEFMQTYEFIIAPVSQVPPFPVEQPYITEINGIPMQNYIEWMRSCYYISVCNL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHCEEEEECCC
PAISVPAGFTRDGLPVGIQIIGRPRADLSVLQLAYAFEQATQHWQRHPQLATG
CCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]