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The map label for this gene is puuB [H]

Identifier: 222523899

GI number: 222523899

Start: 745917

End: 747137

Strand: Reverse

Name: puuB [H]

Synonym: Chy400_0608

Alternate gene names: 222523899

Gene position: 747137-745917 (Counterclockwise)

Preceding gene: 222523900

Following gene: 222523898

Centisome position: 14.18

GC content: 61.26

Gene sequence:

>1221_bases
ATGTTGCGTTCACACTGGCATGCTACCCTTCATCAGGCACCGCTGCCGGGTATCGATCTACCATCAACCGCTGACGTAGT
CGTCGTAGGTGCCGGCGTAGTCGGTGCAGCGACAGCCCTCTGGCTCACACGGGCCGGTCTGCAACCACTCGTTATCGACC
GGCACGGGCCGGCAGCCGGTGCCAGTGGCCACAATGGTGGTATCCTCGCCGCCGGTCTGGCCGAGAATTACCTTGCTGCG
ACTGCCCGTTACGGAGCATCCACCGCACGCGAACTGTACGCTCTCAGTCTGGCCGGACAGCGCTTGATGGTTGATCTGAT
CCAGCAAGAACAGATTGAGTGCGATCTGCGTCTGCACGGCAATCTCAATCTGGCGATTGGTGAAGCACAACTGACGATTG
CCGGCGCAACAGTCGCGGCATTGCAGGGTGATGGCTTTCCGGCGCAGTTGCTCGACCGCACAACTGCCCAGGAGCTGGTG
GCGATTCCGTTAGGGGCAGCCGTCAGTGGCGCCCGCTTCAACCCGGCTGCCGGAACGCTCCATTCCGGGCGATTGGTGTA
CGGGTTGCTGGCCGCTGCGCAACGTCATGGTGCCCTTTGTAGCTGGGGTGTCACCCTGCGCCAGGTAATCACCGCCAACG
GCAGGCTACACCTGATCACAGATCGCGGTACCATCGACACTGCTGCGGCAGTGATTGCCGTTAATGCCTGGAGTGGTGAC
GTGCTACCGGAACTTGCCCGGCGGATTACGATGGTACGCGGGCAGGTACTCTGTACCGTGCCGGTTGAGCCGTTCATTGC
CTGTGGCTTCGGAGCCAGCTTCACTCCTACCGGTGAATATGGTCAACAGACCGTGACCGGACAGGTGTTGTTCGGTGGCT
GTCGGGCTATTGCACCCAATCACGACGTTGGGGTGCAGCCCGGCGAGGTTAGTCCTGAAGTTCAGGGGGCGCTTGAGACC
AGTCTGGGACGTCTCTTCCCAACCCTGGCCGGCATCAACATCGAACGACGCTGGTCGGGGGCAATGGCCTTCACCGCCGA
CTATCTGCCAATCGTGGTCAACCCTGTGCCCGGTCTCTTCGCTATCGGCGGATTCTCCGGTCACGGCATGCCATTTGCGG
CTATTGTTGGTCGCCATCTGGCCGAAGCCGTACAGACCGGTACCATCCCCTCTGCGCTCGCCCCGTTTAGGATCGACCGT
CCAACGTTAGCTGTGGAGTGA

Upstream 100 bases:

>100_bases
GAACAGTTGCGCATCGAAGTACGGTAATCATTGGCCGGTTGGATGAGCATTACGGTACAATACATGGGCTACCGTAGTTC
TCAGACAACAGGATGATTGT

Downstream 100 bases:

>100_bases
TGGTTCAGGACTCCCTGCAACCCGCCCCCACCCGGGCCCGCCCCCGCTGGGGGGGCGGGAGCATATCCCCAGCCCGCCCG
TGCCTCCAGCGGGGGATTAC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLRSHWHATLHQAPLPGIDLPSTADVVVVGAGVVGAATALWLTRAGLQPLVIDRHGPAAGASGHNGGILAAGLAENYLAA
TARYGASTARELYALSLAGQRLMVDLIQQEQIECDLRLHGNLNLAIGEAQLTIAGATVAALQGDGFPAQLLDRTTAQELV
AIPLGAAVSGARFNPAAGTLHSGRLVYGLLAAAQRHGALCSWGVTLRQVITANGRLHLITDRGTIDTAAAVIAVNAWSGD
VLPELARRITMVRGQVLCTVPVEPFIACGFGASFTPTGEYGQQTVTGQVLFGGCRAIAPNHDVGVQPGEVSPEVQGALET
SLGRLFPTLAGINIERRWSGAMAFTADYLPIVVNPVPGLFAIGGFSGHGMPFAAIVGRHLAEAVQTGTIPSALAPFRIDR
PTLAVE

Sequences:

>Translated_406_residues
MLRSHWHATLHQAPLPGIDLPSTADVVVVGAGVVGAATALWLTRAGLQPLVIDRHGPAAGASGHNGGILAAGLAENYLAA
TARYGASTARELYALSLAGQRLMVDLIQQEQIECDLRLHGNLNLAIGEAQLTIAGATVAALQGDGFPAQLLDRTTAQELV
AIPLGAAVSGARFNPAAGTLHSGRLVYGLLAAAQRHGALCSWGVTLRQVITANGRLHLITDRGTIDTAAAVIAVNAWSGD
VLPELARRITMVRGQVLCTVPVEPFIACGFGASFTPTGEYGQQTVTGQVLFGGCRAIAPNHDVGVQPGEVSPEVQGALET
SLGRLFPTLAGINIERRWSGAMAFTADYLPIVVNPVPGLFAIGGFSGHGMPFAAIVGRHLAEAVQTGTIPSALAPFRIDR
PTLAVE
>Mature_406_residues
MLRSHWHATLHQAPLPGIDLPSTADVVVVGAGVVGAATALWLTRAGLQPLVIDRHGPAAGASGHNGGILAAGLAENYLAA
TARYGASTARELYALSLAGQRLMVDLIQQEQIECDLRLHGNLNLAIGEAQLTIAGATVAALQGDGFPAQLLDRTTAQELV
AIPLGAAVSGARFNPAAGTLHSGRLVYGLLAAAQRHGALCSWGVTLRQVITANGRLHLITDRGTIDTAAAVIAVNAWSGD
VLPELARRITMVRGQVLCTVPVEPFIACGFGASFTPTGEYGQQTVTGQVLFGGCRAIAPNHDVGVQPGEVSPEVQGALET
SLGRLFPTLAGINIERRWSGAMAFTADYLPIVVNPVPGLFAIGGFSGHGMPFAAIVGRHLAEAVQTGTIPSALAPFRIDR
PTLAVE

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=396, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41993; Mature: 41993

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSHWHATLHQAPLPGIDLPSTADVVVVGAGVVGAATALWLTRAGLQPLVIDRHGPAAG
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCC
ASGHNGGILAAGLAENYLAATARYGASTARELYALSLAGQRLMVDLIQQEQIECDLRLHG
CCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEC
NLNLAIGEAQLTIAGATVAALQGDGFPAQLLDRTTAQELVAIPLGAAVSGARFNPAAGTL
CEEEEEECEEEEEECEEEEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCC
HSGRLVYGLLAAAQRHGALCSWGVTLRQVITANGRLHLITDRGTIDTAAAVIAVNAWSGD
CCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCC
VLPELARRITMVRGQVLCTVPVEPFIACGFGASFTPTGEYGQQTVTGQVLFGGCRAIAPN
HHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECEEEECCC
HDVGVQPGEVSPEVQGALETSLGRLFPTLAGINIERRWSGAMAFTADYLPIVVNPVPGLF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCEEEEECCCCEE
AIGGFSGHGMPFAAIVGRHLAEAVQTGTIPSALAPFRIDRPTLAVE
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEECC
>Mature Secondary Structure
MLRSHWHATLHQAPLPGIDLPSTADVVVVGAGVVGAATALWLTRAGLQPLVIDRHGPAAG
CCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCC
ASGHNGGILAAGLAENYLAATARYGASTARELYALSLAGQRLMVDLIQQEQIECDLRLHG
CCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEEEEC
NLNLAIGEAQLTIAGATVAALQGDGFPAQLLDRTTAQELVAIPLGAAVSGARFNPAAGTL
CEEEEEECEEEEEECEEEEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCC
HSGRLVYGLLAAAQRHGALCSWGVTLRQVITANGRLHLITDRGTIDTAAAVIAVNAWSGD
CCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCC
VLPELARRITMVRGQVLCTVPVEPFIACGFGASFTPTGEYGQQTVTGQVLFGGCRAIAPN
HHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCCCCCCCCEEEEEEEEEECEEEECCC
HDVGVQPGEVSPEVQGALETSLGRLFPTLAGINIERRWSGAMAFTADYLPIVVNPVPGLF
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEECCCEEEEECCCCEE
AIGGFSGHGMPFAAIVGRHLAEAVQTGTIPSALAPFRIDRPTLAVE
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]