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The map label for this gene is amyB [H]

Identifier: 222523821

GI number: 222523821

Start: 629729

End: 631636

Strand: Reverse

Name: amyB [H]

Synonym: Chy400_0529

Alternate gene names: 222523821

Gene position: 631636-629729 (Counterclockwise)

Preceding gene: 222523822

Following gene: 222523820

Centisome position: 11.99

GC content: 57.44

Gene sequence:

>1908_bases
GTGGAACAGTATCACGTCTCTGCCGATTTTATCTTCGGGACTATGGCGACCGATAGTCGGCGATTGGGGTACGTGCGGGC
CGAGTTAGCCGGTATGTCGCACCGCTACCGGATGGAGCCGCTCGCTCCACAGCCGGGCGAACCGGTGCGGCTCTTCGTCA
CCCTTGGCCCGTCAATTGTCGCCGATCAGGTGTGTGTCTACTACACAACAGATGGTAGTGAGCCTCACGGTGAACGAGGT
CAGCCGGCGCCGGGAAGTCACGTAGCGCACGGACAACGTCTCTCCAGTCATTGGGATACCCTGCTCTGGGGATACGCCGA
GGAGTGGGCAATTGATCTCCCACCACAACCAGCCGACACGGTGGTTCGTTACCGGATTGAAGCCTGGCTTCGTGAGGGGA
ACGGCTCACTCTGGTACAGCGAAGTGATCGGCGCAACTGCGGGTGACGGTAGTGTTGTTGGGCAACCAGGGCCGGGCGAT
CACTATCTGCACGAGATGGGGTATGAATTCAACCTGACGTTCTTCCGCCGTCCACGTACCTGTGCGTATCGGGTTGGCGA
TGAGGGAGTACCGGGATGGCTTGATGATGCGCTCATTTATCACGTCTTCATTGATCGTTTTCATCCTGGTCCTGGCCGAC
AGTTTGCCAATCCGCCAACTCCGATGGGGATTTACGGTGGCACTCTCGCCGGTGTAACCAGCAATCTGGATTACATTGCC
AGCCTGGGCACGACGACGATCTGGCTCTCGCCACTCTTCCCTTCACCCTCTCACCACGGCTATGATGCGACCGACTATTA
CAGTGTCGAGCCACGCCTGGGTACCATGGCCGACCTGCAAACGCTCATTGCTGCGGCCCATGACCGGGGAATGCGGGTGA
TCTTTGATTACACCGCCAATCATTTCTCGAACAGACATCCTATCTTTCAGCGAGCGATTAGCGATCCTCACAGCCCAGAA
CGAGACTGGTTTATCTTTACCCGTTATCCTGATCTGTATGTGTCGTTCTTTGGGGTTGCCGAATTACCGCAACTCGATCT
GGACTACCCACCAGCCCGCCAGTTTATGATTGATGCCGCCCGTTACTGGCTGGAACAGGGGGTTGATGGGTATCGCCTCG
ACTATACCATTGGCCCCTCACACGATTTCTGGACATTATTCCGGGCGGCAATGCGCGCCGTCAGGCCCGATTGTGTGCTC
ATCGGTGAAGCTGTGGATACGGCAGAGGTGCTACGCTCGTATGTTGGCCGTCTCGATGGCTGTCTGGATTTTCTGCTGCT
GCAAGCCATCCGCCGCTTTTTCGCCTTCGATGAAATACGTGCCAGCCAGTTTGCTGTCTTTCTCAACCGCCATCTGAACT
ACTTTCCACCCGGCTTCAGTTTGCCGACATTTCTCGACAATCACGACATGAATCGGTTTTTGTGGGTGGTACGCGGTGAC
ACACGTCGTCTGCGGCTCGCGGCACTTTGTCAGTATACCCTGCCCCATCCACCGGTACTCTACTACGGCACTGAAGTTGG
CCTTAGCCAACGGCTCGATGTGCGTCACGCCGACGGAAGTGGTCACCCGGAAGAATCGCGGTTACCGATGCGCTGGGGAG
ATGAACAGGATCAGGAGTTGCTCGATTTCTACCGGCAACTGGGCGCGCTGCGACGGGCAACGACCGCGGTCTGGCGAGGT
AAGCGGACTATCACCCATATTGATGATCAGCGTGGACACCTGGCGTATACCTGCACGGCTCAAGACCAGACCTGGCTCGT
TGTGCTGAATAATGCCTCACAGGCTGGCGTCATTCCCGTACCTCCAGGTCGCTGGACGGTTGTCCTGCCGAGCCAGACTG
CCGAACTTGTGGCCGGAAAGGTCGCGCTGCCACCGTATGGTGGGGCGATCTTACGTCGCGAAGCGTAG

Upstream 100 bases:

>100_bases
TATGAAAGATAGCGCATCCCATCCCATGTTGCTCCCCTCTGGTTCTGCATAATGTCAATCGTTTTGTCCTGATATACGTC
AACTCAAAGGATGGTTCATC

Downstream 100 bases:

>100_bases
CCAGTTGCACATTCATCGGCGATCCGGTATACTATCGTTTCGGTGTAAGAATAACGGCGCATAATCGCTGCTACGGGGCG
CACCAAATCTACAGCGAAGG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MEQYHVSADFIFGTMATDSRRLGYVRAELAGMSHRYRMEPLAPQPGEPVRLFVTLGPSIVADQVCVYYTTDGSEPHGERG
QPAPGSHVAHGQRLSSHWDTLLWGYAEEWAIDLPPQPADTVVRYRIEAWLREGNGSLWYSEVIGATAGDGSVVGQPGPGD
HYLHEMGYEFNLTFFRRPRTCAYRVGDEGVPGWLDDALIYHVFIDRFHPGPGRQFANPPTPMGIYGGTLAGVTSNLDYIA
SLGTTTIWLSPLFPSPSHHGYDATDYYSVEPRLGTMADLQTLIAAAHDRGMRVIFDYTANHFSNRHPIFQRAISDPHSPE
RDWFIFTRYPDLYVSFFGVAELPQLDLDYPPARQFMIDAARYWLEQGVDGYRLDYTIGPSHDFWTLFRAAMRAVRPDCVL
IGEAVDTAEVLRSYVGRLDGCLDFLLLQAIRRFFAFDEIRASQFAVFLNRHLNYFPPGFSLPTFLDNHDMNRFLWVVRGD
TRRLRLAALCQYTLPHPPVLYYGTEVGLSQRLDVRHADGSGHPEESRLPMRWGDEQDQELLDFYRQLGALRRATTAVWRG
KRTITHIDDQRGHLAYTCTAQDQTWLVVLNNASQAGVIPVPPGRWTVVLPSQTAELVAGKVALPPYGGAILRREA

Sequences:

>Translated_635_residues
MEQYHVSADFIFGTMATDSRRLGYVRAELAGMSHRYRMEPLAPQPGEPVRLFVTLGPSIVADQVCVYYTTDGSEPHGERG
QPAPGSHVAHGQRLSSHWDTLLWGYAEEWAIDLPPQPADTVVRYRIEAWLREGNGSLWYSEVIGATAGDGSVVGQPGPGD
HYLHEMGYEFNLTFFRRPRTCAYRVGDEGVPGWLDDALIYHVFIDRFHPGPGRQFANPPTPMGIYGGTLAGVTSNLDYIA
SLGTTTIWLSPLFPSPSHHGYDATDYYSVEPRLGTMADLQTLIAAAHDRGMRVIFDYTANHFSNRHPIFQRAISDPHSPE
RDWFIFTRYPDLYVSFFGVAELPQLDLDYPPARQFMIDAARYWLEQGVDGYRLDYTIGPSHDFWTLFRAAMRAVRPDCVL
IGEAVDTAEVLRSYVGRLDGCLDFLLLQAIRRFFAFDEIRASQFAVFLNRHLNYFPPGFSLPTFLDNHDMNRFLWVVRGD
TRRLRLAALCQYTLPHPPVLYYGTEVGLSQRLDVRHADGSGHPEESRLPMRWGDEQDQELLDFYRQLGALRRATTAVWRG
KRTITHIDDQRGHLAYTCTAQDQTWLVVLNNASQAGVIPVPPGRWTVVLPSQTAELVAGKVALPPYGGAILRREA
>Mature_635_residues
MEQYHVSADFIFGTMATDSRRLGYVRAELAGMSHRYRMEPLAPQPGEPVRLFVTLGPSIVADQVCVYYTTDGSEPHGERG
QPAPGSHVAHGQRLSSHWDTLLWGYAEEWAIDLPPQPADTVVRYRIEAWLREGNGSLWYSEVIGATAGDGSVVGQPGPGD
HYLHEMGYEFNLTFFRRPRTCAYRVGDEGVPGWLDDALIYHVFIDRFHPGPGRQFANPPTPMGIYGGTLAGVTSNLDYIA
SLGTTTIWLSPLFPSPSHHGYDATDYYSVEPRLGTMADLQTLIAAAHDRGMRVIFDYTANHFSNRHPIFQRAISDPHSPE
RDWFIFTRYPDLYVSFFGVAELPQLDLDYPPARQFMIDAARYWLEQGVDGYRLDYTIGPSHDFWTLFRAAMRAVRPDCVL
IGEAVDTAEVLRSYVGRLDGCLDFLLLQAIRRFFAFDEIRASQFAVFLNRHLNYFPPGFSLPTFLDNHDMNRFLWVVRGD
TRRLRLAALCQYTLPHPPVLYYGTEVGLSQRLDVRHADGSGHPEESRLPMRWGDEQDQELLDFYRQLGALRRATTAVWRG
KRTITHIDDQRGHLAYTCTAQDQTWLVVLNNASQAGVIPVPPGRWTVVLPSQTAELVAGKVALPPYGGAILRREA

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=383, Percent_Identity=24.0208877284595, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI1786604, Length=501, Percent_Identity=26.7465069860279, Blast_Score=137, Evalue=3e-33,
Organism=Escherichia coli, GI1790687, Length=217, Percent_Identity=29.4930875576037, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1789995, Length=128, Percent_Identity=33.59375, Blast_Score=75, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=215, Percent_Identity=32.5581395348837, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322241, Length=218, Percent_Identity=30.7339449541284, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322021, Length=218, Percent_Identity=30.7339449541284, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324416, Length=218, Percent_Identity=30.2752293577982, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6321731, Length=218, Percent_Identity=31.1926605504587, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319776, Length=218, Percent_Identity=31.1926605504587, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321726, Length=218, Percent_Identity=29.8165137614679, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24586591, Length=436, Percent_Identity=24.7706422018349, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24586587, Length=429, Percent_Identity=24.4755244755245, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24583745, Length=392, Percent_Identity=25, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI45549022, Length=416, Percent_Identity=25.7211538461538, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24586593, Length=567, Percent_Identity=22.5749559082892, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI221330053, Length=405, Percent_Identity=24.1975308641975, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24583747, Length=177, Percent_Identity=33.8983050847458, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24583749, Length=177, Percent_Identity=33.8983050847458, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24586599, Length=217, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24586597, Length=225, Percent_Identity=28.4444444444444, Blast_Score=90, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24586589, Length=434, Percent_Identity=23.2718894009217, Blast_Score=86, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 71699; Mature: 71699

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYHVSADFIFGTMATDSRRLGYVRAELAGMSHRYRMEPLAPQPGEPVRLFVTLGPSIV
CCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEEEECCCHH
ADQVCVYYTTDGSEPHGERGQPAPGSHVAHGQRLSSHWDTLLWGYAEEWAIDLPPQPADT
HCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCEEECCCCCHHH
VVRYRIEAWLREGNGSLWYSEVIGATAGDGSVVGQPGPGDHYLHEMGYEFNLTFFRRPRT
HHHHHHHHHHHCCCCCEEEHHHHCCCCCCCCEECCCCCCHHHHHHCCCEEEEEEEECCCC
CAYRVGDEGVPGWLDDALIYHVFIDRFHPGPGRQFANPPTPMGIYGGTLAGVTSNLDYIA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHH
SLGTTTIWLSPLFPSPSHHGYDATDYYSVEPRLGTMADLQTLIAAAHDRGMRVIFDYTAN
HCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECHH
HFSNRHPIFQRAISDPHSPERDWFIFTRYPDLYVSFFGVAELPQLDLDYPPARQFMIDAA
HCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHCHHCCCCCCCCCCCHHHHHHHHH
RYWLEQGVDGYRLDYTIGPSHDFWTLFRAAMRAVRPDCVLIGEAVDTAEVLRSYVGRLDG
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
CLDFLLLQAIRRFFAFDEIRASQFAVFLNRHLNYFPPGFSLPTFLDNHDMNRFLWVVRGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCEEEEEEECC
TRRLRLAALCQYTLPHPPVLYYGTEVGLSQRLDVRHADGSGHPEESRLPMRWGDEQDQEL
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHH
LDFYRQLGALRRATTAVWRGKRTITHIDDQRGHLAYTCTAQDQTWLVVLNNASQAGVIPV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCEEEC
PPGRWTVVLPSQTAELVAGKVALPPYGGAILRREA
CCCCEEEEECCCCHHHHCCCEECCCCCCCEEEECC
>Mature Secondary Structure
MEQYHVSADFIFGTMATDSRRLGYVRAELAGMSHRYRMEPLAPQPGEPVRLFVTLGPSIV
CCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEEEECCCHH
ADQVCVYYTTDGSEPHGERGQPAPGSHVAHGQRLSSHWDTLLWGYAEEWAIDLPPQPADT
HCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCEEECCCCCHHH
VVRYRIEAWLREGNGSLWYSEVIGATAGDGSVVGQPGPGDHYLHEMGYEFNLTFFRRPRT
HHHHHHHHHHHCCCCCEEEHHHHCCCCCCCCEECCCCCCHHHHHHCCCEEEEEEEECCCC
CAYRVGDEGVPGWLDDALIYHVFIDRFHPGPGRQFANPPTPMGIYGGTLAGVTSNLDYIA
EEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHH
SLGTTTIWLSPLFPSPSHHGYDATDYYSVEPRLGTMADLQTLIAAAHDRGMRVIFDYTAN
HCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEECHH
HFSNRHPIFQRAISDPHSPERDWFIFTRYPDLYVSFFGVAELPQLDLDYPPARQFMIDAA
HCCCCCHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHCHHCCCCCCCCCCCHHHHHHHHH
RYWLEQGVDGYRLDYTIGPSHDFWTLFRAAMRAVRPDCVLIGEAVDTAEVLRSYVGRLDG
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHH
CLDFLLLQAIRRFFAFDEIRASQFAVFLNRHLNYFPPGFSLPTFLDNHDMNRFLWVVRGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCEEEEEEECC
TRRLRLAALCQYTLPHPPVLYYGTEVGLSQRLDVRHADGSGHPEESRLPMRWGDEQDQEL
CCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHH
LDFYRQLGALRRATTAVWRGKRTITHIDDQRGHLAYTCTAQDQTWLVVLNNASQAGVIPV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCCEEEEEEECCCCCCEEEC
PPGRWTVVLPSQTAELVAGKVALPPYGGAILRREA
CCCCEEEEECCCCHHHHCCCEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2458257 [H]