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The map label for this gene is hyi [C]

Identifier: 222523688

GI number: 222523688

Start: 464835

End: 465605

Strand: Reverse

Name: hyi [C]

Synonym: Chy400_0394

Alternate gene names: 222523688

Gene position: 465605-464835 (Counterclockwise)

Preceding gene: 222523689

Following gene: 222523687

Centisome position: 8.84

GC content: 56.68

Gene sequence:

>771_bases
ATGTCGGCCCTCGCACCCCTCCGCTTCTCAGTCTGTCTCGATATGATCTTCACCGATCGCCCGTTTGAGCAGCGACTCGA
ACACATTGCCGATCTCGGCTTCCGCGCCTATGAATTCTGGACGCGGAAGGGCAAGGACATGAACATCACCATTGCTCTGC
AAACCGCCTTACGCCTGCATTGTGTGGCTATTTTCGGCAGCGAAGCAGCCCCCAATGATCCCGATCAGCGAGCACGCTTT
CGTGATGAAGTGACGCGCAGTGCCGCGCTGGCAGTTGATCTGGCCTGTGATAACCTCATCGTTCACGGTGGGATTACCCG
GACAGACCTCGATTATGCCACGCAACGCGCCCATCTGGTTGATGCGCTGCATGAGGCTGCCGAGACTGCGGCTGATGCCG
GTCTTAATCTACTCCTCGAACCGCGCAACCCGGTCGATCATCCGGGTAGTTTTCTCTGGTCATCTGACGAAGGCTTTGCC
ATCGTGCGCGAGCTTGGTCAACCGCACGTCAAACTGTTGTTTGATTGTTACCATCAACAGATCAGCGAAGGAAACCTGAC
CCGTCGCATCCTGGCCAATCTCGATCTTATCGGCCACATCCACGTCGCCGACGTCCCCGGTCGCCATGAGCCGGGCACCG
GTGAAATCAATTACGAGCACATCTTTGGCGTTTTGCGTGAACATGGGTATAGTGGCTATGTCGGTCTTGAATATACCCCG
CGAATCGATGCGGCGGCCAGTCTGCGGGTGGTGCGGATGATGAGTCAGTGA

Upstream 100 bases:

>100_bases
ACGATTTGACCGACCAGCCAGTCAGATGCATCATGATCGCCAGCGCTGCAACGGGTCGGTTGCGCCATAGGAGCTCTTGT
GCAGAAAAGTGAGGTTGAAG

Downstream 100 bases:

>100_bases
GCGTGAAGGGTGACATATTGTCACTACTGTGGTTCACTCAGAATGCTATAATAAAGCTAGCCAACGTTCCGACACTGCAT
CGTAATCACTTTCTGGAGGC

Product: xylose isomerase domain-containing protein

Products: 2-hydroxy-3-oxopropanoate

Alternate protein names: ORF4 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSALAPLRFSVCLDMIFTDRPFEQRLEHIADLGFRAYEFWTRKGKDMNITIALQTALRLHCVAIFGSEAAPNDPDQRARF
RDEVTRSAALAVDLACDNLIVHGGITRTDLDYATQRAHLVDALHEAAETAADAGLNLLLEPRNPVDHPGSFLWSSDEGFA
IVRELGQPHVKLLFDCYHQQISEGNLTRRILANLDLIGHIHVADVPGRHEPGTGEINYEHIFGVLREHGYSGYVGLEYTP
RIDAAASLRVVRMMSQ

Sequences:

>Translated_256_residues
MSALAPLRFSVCLDMIFTDRPFEQRLEHIADLGFRAYEFWTRKGKDMNITIALQTALRLHCVAIFGSEAAPNDPDQRARF
RDEVTRSAALAVDLACDNLIVHGGITRTDLDYATQRAHLVDALHEAAETAADAGLNLLLEPRNPVDHPGSFLWSSDEGFA
IVRELGQPHVKLLFDCYHQQISEGNLTRRILANLDLIGHIHVADVPGRHEPGTGEINYEHIFGVLREHGYSGYVGLEYTP
RIDAAASLRVVRMMSQ
>Mature_255_residues
SALAPLRFSVCLDMIFTDRPFEQRLEHIADLGFRAYEFWTRKGKDMNITIALQTALRLHCVAIFGSEAAPNDPDQRARFR
DEVTRSAALAVDLACDNLIVHGGITRTDLDYATQRAHLVDALHEAAETAADAGLNLLLEPRNPVDHPGSFLWSSDEGFAI
VRELGQPHVKLLFDCYHQQISEGNLTRRILANLDLIGHIHVADVPGRHEPGTGEINYEHIFGVLREHGYSGYVGLEYTPR
IDAAASLRVVRMMSQ

Specific function: Catalyzes The Reversible Isomerization Between Hydroxypyruvate And 2-Hydroxy-3-Oxopropanoate (Also Termed Tartronate Semialdehyde). [C]

COG id: COG3622

COG function: function code G; Hydroxypyruvate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hyi family [H]

Homologues:

Organism=Homo sapiens, GI300068962, Length=267, Percent_Identity=31.0861423220974, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI300068964, Length=250, Percent_Identity=30.4, Blast_Score=104, Evalue=6e-23,
Organism=Escherichia coli, GI1786718, Length=253, Percent_Identity=33.201581027668, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1789095, Length=245, Percent_Identity=30.2040816326531, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI87081874, Length=123, Percent_Identity=37.3983739837398, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17552022, Length=263, Percent_Identity=26.615969581749, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17530883, Length=262, Percent_Identity=26.3358778625954, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: 5.3.1.22

Molecular weight: Translated: 28581; Mature: 28450

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALAPLRFSVCLDMIFTDRPFEQRLEHIADLGFRAYEFWTRKGKDMNITIALQTALRLH
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEHHHHHH
CVAIFGSEAAPNDPDQRARFRDEVTRSAALAVDLACDNLIVHGGITRTDLDYATQRAHLV
HHEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCHHHHHHHHHHHH
DALHEAAETAADAGLNLLLEPRNPVDHPGSFLWSSDEGFAIVRELGQPHVKLLFDCYHQQ
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHH
ISEGNLTRRILANLDLIGHIHVADVPGRHEPGTGEINYEHIFGVLREHGYSGYVGLEYTP
HCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCEECC
RIDAAASLRVVRMMSQ
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SALAPLRFSVCLDMIFTDRPFEQRLEHIADLGFRAYEFWTRKGKDMNITIALQTALRLH
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEHHHHHH
CVAIFGSEAAPNDPDQRARFRDEVTRSAALAVDLACDNLIVHGGITRTDLDYATQRAHLV
HHEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCCCHHHHHHHHHHHH
DALHEAAETAADAGLNLLLEPRNPVDHPGSFLWSSDEGFAIVRELGQPHVKLLFDCYHQQ
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEECCCCHHHHHHHCCHHHHHHHHHHHHH
ISEGNLTRRILANLDLIGHIHVADVPGRHEPGTGEINYEHIFGVLREHGYSGYVGLEYTP
HCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCEECC
RIDAAASLRVVRMMSQ
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {hydroxypyruvate}} [C]

Substrates: hydroxypyruvate

Specific reaction: hydroxypyruvate = 2-hydroxy-3-oxopropanoate; Other Name(s): hydroxypyruvate ketol-isomerase

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: Cu2+; Fe2+; Hydroxy l amine; Iodoacetate; NaCN [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7851418 [H]