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The map label for this gene is purR [H]

Identifier: 222523686

GI number: 222523686

Start: 462173

End: 463213

Strand: Reverse

Name: purR [H]

Synonym: Chy400_0392

Alternate gene names: 222523686

Gene position: 463213-462173 (Counterclockwise)

Preceding gene: 222523687

Following gene: 222523685

Centisome position: 8.79

GC content: 45.73

Gene sequence:

>1041_bases
ATGAAACGTCCCACGCAAATGGATGTTGCCAGGCGCGCAGGGGTCTCGCGAGCAACAGTTTCTCACGTAATCAATGGTCT
GTCTGGGGGACGGGTTCCTATTTCTCCAGAGACTCGGGAACGTGTCTGGCAAGCAATTAAAGAATTAGGATATGAACCTG
ATGCCGGAGCCAGAGCGTTACGATTGGGTAAGTCAAGGACCATCGGACTAATTATGCCAGATTTGTATAATCCTCATTTT
TGGGAAAATGCTGAAGGTGTAGAGCAGGAAGCACGTGCAAATGGATATCGGTTGCTTCTTTGCAGTATGAACCTAAACAT
TCAATATGGTGAAGATGCATTCAAGGATTTAGTTGGCCGGCGAATTGATGCATTGATCTTAATGGGTTCATTTGTTTACG
AGTCAGAAGAAGCCAGGAATACATTAATCCGTAGCTTAGAGCGAGGTTTACCCATTGTGGAAATAAGTGATAGAGTTACC
AAAGATTACCTGGCAGATTGTGTATTGTCTGATTATCGTGCAGTAGCAGCAGCAGCGATGCAACATCTCATAATGCTAGG
ACACCGACGAATAGGTCTTATCTACGGTGTAGCTAATTCGAGCTTAGCAGAAGATCGTCTGATACCCTACAAGAAAAGTC
TTCAGGCTGTTGGGATACCAATTGATGAGCAGCTTATCGTACATTGTGGTCCAACTATTGAGGAAGGATATCGGGCAGCC
ATTCACCTTTTACAGAAACCGAATCGGCCAACAGCGATTGTAGCAATAAACGATTTATTGGCCATAGCAGTTTTGCGTGC
GGCTGGTGATATGGGGCTACGAGTTCCTGCTGATGTATCGTTGATAGGTTTCGATGATATTGCAATTGCTAATTATCTCA
TTCCTCGCCTGACAACTGCGGCAAAAGATGCAGTGCAACTCGGTCGTGAAGCGGTGAAATTGGCTTTGGCAAGGTTGCGT
GATCCAAAACGACCAAGACAAGTTATCGAGGTGCCAGCTCGACTTATTTTGCGTGAGTCGACCGCACCACCATCGTGTTG
A

Upstream 100 bases:

>100_bases
TTCATGATATTGTGTAGTTATTAAATTGACACGTGTAAGTTAACTCGTGTAAATAATGAATCTGCCTCCCTTCCAGCCTT
AATAGTTAGGAGGCGTATGT

Downstream 100 bases:

>100_bases
TACACGTGTAAAGAGTGTATACAGGTGTTAGGAGATGCCGATGATCTTGGCGGATGGCGGCATCTCCTACAGTCGGACAT
ACCGATCGATGCCCAGGTCG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MKRPTQMDVARRAGVSRATVSHVINGLSGGRVPISPETRERVWQAIKELGYEPDAGARALRLGKSRTIGLIMPDLYNPHF
WENAEGVEQEARANGYRLLLCSMNLNIQYGEDAFKDLVGRRIDALILMGSFVYESEEARNTLIRSLERGLPIVEISDRVT
KDYLADCVLSDYRAVAAAAMQHLIMLGHRRIGLIYGVANSSLAEDRLIPYKKSLQAVGIPIDEQLIVHCGPTIEEGYRAA
IHLLQKPNRPTAIVAINDLLAIAVLRAAGDMGLRVPADVSLIGFDDIAIANYLIPRLTTAAKDAVQLGREAVKLALARLR
DPKRPRQVIEVPARLILRESTAPPSC

Sequences:

>Translated_346_residues
MKRPTQMDVARRAGVSRATVSHVINGLSGGRVPISPETRERVWQAIKELGYEPDAGARALRLGKSRTIGLIMPDLYNPHF
WENAEGVEQEARANGYRLLLCSMNLNIQYGEDAFKDLVGRRIDALILMGSFVYESEEARNTLIRSLERGLPIVEISDRVT
KDYLADCVLSDYRAVAAAAMQHLIMLGHRRIGLIYGVANSSLAEDRLIPYKKSLQAVGIPIDEQLIVHCGPTIEEGYRAA
IHLLQKPNRPTAIVAINDLLAIAVLRAAGDMGLRVPADVSLIGFDDIAIANYLIPRLTTAAKDAVQLGREAVKLALARLR
DPKRPRQVIEVPARLILRESTAPPSC
>Mature_346_residues
MKRPTQMDVARRAGVSRATVSHVINGLSGGRVPISPETRERVWQAIKELGYEPDAGARALRLGKSRTIGLIMPDLYNPHF
WENAEGVEQEARANGYRLLLCSMNLNIQYGEDAFKDLVGRRIDALILMGSFVYESEEARNTLIRSLERGLPIVEISDRVT
KDYLADCVLSDYRAVAAAAMQHLIMLGHRRIGLIYGVANSSLAEDRLIPYKKSLQAVGIPIDEQLIVHCGPTIEEGYRAA
IHLLQKPNRPTAIVAINDLLAIAVLRAAGDMGLRVPADVSLIGFDDIAIANYLIPRLTTAAKDAVQLGREAVKLALARLR
DPKRPRQVIEVPARLILRESTAPPSC

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=37.0588235294118, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1790369, Length=320, Percent_Identity=35.3125, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=32.5443786982249, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1789202, Length=342, Percent_Identity=34.2105263157895, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1789068, Length=345, Percent_Identity=32.7536231884058, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788474, Length=338, Percent_Identity=31.3609467455621, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1787580, Length=344, Percent_Identity=28.4883720930233, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786540, Length=350, Percent_Identity=27.7142857142857, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787906, Length=344, Percent_Identity=28.4883720930233, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI48994940, Length=328, Percent_Identity=28.6585365853659, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789456, Length=346, Percent_Identity=25.4335260115607, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786268, Length=323, Percent_Identity=25.077399380805, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790689, Length=353, Percent_Identity=23.2294617563739, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38123; Mature: 38123

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRPTQMDVARRAGVSRATVSHVINGLSGGRVPISPETRERVWQAIKELGYEPDAGARAL
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
RLGKSRTIGLIMPDLYNPHFWENAEGVEQEARANGYRLLLCSMNLNIQYGEDAFKDLVGR
HCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCEEEEEEEECCEEEECHHHHHHHHHH
RIDALILMGSFVYESEEARNTLIRSLERGLPIVEISDRVTKDYLADCVLSDYRAVAAAAM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
QHLIMLGHRRIGLIYGVANSSLAEDRLIPYKKSLQAVGIPIDEQLIVHCGPTIEEGYRAA
HHHHHHCCCCEEEEEEECCCCHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
IHLLQKPNRPTAIVAINDLLAIAVLRAAGDMGLRVPADVSLIGFDDIAIANYLIPRLTTA
HHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
AKDAVQLGREAVKLALARLRDPKRPRQVIEVPARLILRESTAPPSC
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKRPTQMDVARRAGVSRATVSHVINGLSGGRVPISPETRERVWQAIKELGYEPDAGARAL
CCCCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHH
RLGKSRTIGLIMPDLYNPHFWENAEGVEQEARANGYRLLLCSMNLNIQYGEDAFKDLVGR
HCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHCCCEEEEEEEECCEEEECHHHHHHHHHH
RIDALILMGSFVYESEEARNTLIRSLERGLPIVEISDRVTKDYLADCVLSDYRAVAAAAM
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHH
QHLIMLGHRRIGLIYGVANSSLAEDRLIPYKKSLQAVGIPIDEQLIVHCGPTIEEGYRAA
HHHHHHCCCCEEEEEEECCCCHHHHCCCCHHHHHHHCCCCCCCCEEEECCCCHHHHHHHH
IHLLQKPNRPTAIVAINDLLAIAVLRAAGDMGLRVPADVSLIGFDDIAIANYLIPRLTTA
HHHHHCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHH
AKDAVQLGREAVKLALARLRDPKRPRQVIEVPARLILRESTAPPSC
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA