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The map label for this gene is pfkA

Identifier: 222523568

GI number: 222523568

Start: 330129

End: 331199

Strand: Reverse

Name: pfkA

Synonym: Chy400_0274

Alternate gene names: 222523568

Gene position: 331199-330129 (Counterclockwise)

Preceding gene: 222523569

Following gene: 222523567

Centisome position: 6.29

GC content: 56.49

Gene sequence:

>1071_bases
ATGGCGAGCAAAAAGCAGCGGATCGGCGTGCTTACCAGTGGCGGTGACGCGCCAGGACTCAATGCCGTCATCCGTGCAGT
CGTCAAGTCGGCGTCCGGTCTGGGTTGGGAAGTGATCGGTATTCACGATGGTTTTGAGGGTTTATTAGGGACAAAGAGCT
ACCGGGTACTTACCAATGCTGATGTACAGGGATTATTGCCGCGGGGTGGCACAATCCTGCGCACAACCAACAAGGGGCAT
TTTGGCCCACGCCGGAGCGATGAGCTGTCAGAGGCCGATCCGTATGTTCGTGCGGTAAAGGCGATTGAGGAGATGGGGCT
GCGGGCCTTGATCACTATTGGTGGTGAGGGGACGCAGCGGATTGCTCTTGAACTGCATAAATTGGGTGCACCGGTGATCG
GCGTACCGAAAACGATTGATAACGATCTTGCCGGAACTGATCGTACATTCGGTTTCGATACAGCGTTGCAGGTAGCAACC
GATGCGATTGATCGGTTACACACCACTGCTGCCAGCCATAATCGCGTCATGGTGTTGGAAGTTATGGGCCGGCATACCGG
TTGGATCGCTCTCCACGCCGGTCTGGCTGGTGGTGCAGATGTCATCTTGATTCCGGAAATTCCATTTTCGATTGAGCGAG
TCGCCGAGAAGGTTATGGCGCGTGACCAGCAGGGTAGCTCGTTCAGCATCATCGTTGTGGCTGAAGGTGCCCGCCCTCGT
GGTGGTAGCGAGATGTATATCGCCGAAGGCCGGTTGGGTGGAATTGGTCACTGGGTTGGTGAACAGCTCGAAAAGCTGAC
CGCCAAAGAGGTTCGGGTTGTCGTTTTGGGACATTTACAGCGTGGTGGTTCGCCATCGCCTTATGATCGCCTGCTTTCGA
CCCGCTATGGTGCAGCAGCAGTGCAGGCGGCAGCTCGTGGCATCTATGGCGAAATGGTGGCTCTCCGCGGGCAAGACATT
GTAACGGTGCCGTTGGCCGAAGCCTGCGGCCACCTGAATCGGGTACGTCCTCACAGCGACCTGGTGCTCTGTGCACGGAG
TTTGGGGATTGCCTTTGGCGATGAACTCTGA

Upstream 100 bases:

>100_bases
ACAACCCGAAAAAACCAGTCTTTCACTCGCAAACATAGTTGCGAGTGGTATAATCTGTTTGTCGGGTTGAAACGATAACG
CAAATCCAAAGGGGTAAGCC

Downstream 100 bases:

>100_bases
TCACATGGTATGCTTAGCGACAGGATAAAGGTATGACGAGATGATCCGCTACGAGATCGCCGCAACGACTGTAACCACGG
CGCCGCCGGAGCGGGTGCTG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MASKKQRIGVLTSGGDAPGLNAVIRAVVKSASGLGWEVIGIHDGFEGLLGTKSYRVLTNADVQGLLPRGGTILRTTNKGH
FGPRRSDELSEADPYVRAVKAIEEMGLRALITIGGEGTQRIALELHKLGAPVIGVPKTIDNDLAGTDRTFGFDTALQVAT
DAIDRLHTTAASHNRVMVLEVMGRHTGWIALHAGLAGGADVILIPEIPFSIERVAEKVMARDQQGSSFSIIVVAEGARPR
GGSEMYIAEGRLGGIGHWVGEQLEKLTAKEVRVVVLGHLQRGGSPSPYDRLLSTRYGAAAVQAAARGIYGEMVALRGQDI
VTVPLAEACGHLNRVRPHSDLVLCARSLGIAFGDEL

Sequences:

>Translated_356_residues
MASKKQRIGVLTSGGDAPGLNAVIRAVVKSASGLGWEVIGIHDGFEGLLGTKSYRVLTNADVQGLLPRGGTILRTTNKGH
FGPRRSDELSEADPYVRAVKAIEEMGLRALITIGGEGTQRIALELHKLGAPVIGVPKTIDNDLAGTDRTFGFDTALQVAT
DAIDRLHTTAASHNRVMVLEVMGRHTGWIALHAGLAGGADVILIPEIPFSIERVAEKVMARDQQGSSFSIIVVAEGARPR
GGSEMYIAEGRLGGIGHWVGEQLEKLTAKEVRVVVLGHLQRGGSPSPYDRLLSTRYGAAAVQAAARGIYGEMVALRGQDI
VTVPLAEACGHLNRVRPHSDLVLCARSLGIAFGDEL
>Mature_355_residues
ASKKQRIGVLTSGGDAPGLNAVIRAVVKSASGLGWEVIGIHDGFEGLLGTKSYRVLTNADVQGLLPRGGTILRTTNKGHF
GPRRSDELSEADPYVRAVKAIEEMGLRALITIGGEGTQRIALELHKLGAPVIGVPKTIDNDLAGTDRTFGFDTALQVATD
AIDRLHTTAASHNRVMVLEVMGRHTGWIALHAGLAGGADVILIPEIPFSIERVAEKVMARDQQGSSFSIIVVAEGARPRG
GSEMYIAEGRLGGIGHWVGEQLEKLTAKEVRVVVLGHLQRGGSPSPYDRLLSTRYGAAAVQAAARGIYGEMVALRGQDIV
TVPLAEACGHLNRVRPHSDLVLCARSLGIAFGDEL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI266453768, Length=355, Percent_Identity=34.9295774647887, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI266453748, Length=355, Percent_Identity=34.9295774647887, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI4505749, Length=355, Percent_Identity=34.9295774647887, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI266453619, Length=355, Percent_Identity=34.9295774647887, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI48762920, Length=367, Percent_Identity=34.6049046321526, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI11321601, Length=369, Percent_Identity=33.3333333333333, Blast_Score=161, Evalue=8e-40,
Organism=Escherichia coli, GI1790350, Length=344, Percent_Identity=38.953488372093, Blast_Score=229, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI25147584, Length=352, Percent_Identity=33.2386363636364, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17558788, Length=391, Percent_Identity=30.690537084399, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI72003601, Length=313, Percent_Identity=28.4345047923323, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321679, Length=369, Percent_Identity=33.0623306233062, Blast_Score=169, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6323861, Length=362, Percent_Identity=32.8729281767956, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24652337, Length=357, Percent_Identity=33.6134453781513, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI17647809, Length=355, Percent_Identity=33.8028169014084, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI28573326, Length=357, Percent_Identity=33.0532212885154, Blast_Score=178, Evalue=4e-45,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_CHLAA (A9WCU2)

Other databases:

- EMBL:   CP000909
- RefSeq:   YP_001633900.1
- ProteinModelPortal:   A9WCU2
- SMR:   A9WCU2
- GeneID:   5824865
- GenomeReviews:   CP000909_GR
- KEGG:   cau:Caur_0258
- HOGENOM:   HBG405044
- OMA:   MVALRGT
- ProtClustDB:   CLSK983903
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02483

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 37704; Mature: 37573

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 140-140 BINDING 175-175 BINDING 272-272 BINDING 278-278 BINDING 281-281

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKKQRIGVLTSGGDAPGLNAVIRAVVKSASGLGWEVIGIHDGFEGLLGTKSYRVLTNA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCEEEEECC
DVQGLLPRGGTILRTTNKGHFGPRRSDELSEADPYVRAVKAIEEMGLRALITIGGEGTQR
CCEEECCCCCEEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCEEEEEECCCCCHH
IALELHKLGAPVIGVPKTIDNDLAGTDRTFGFDTALQVATDAIDRLHTTAASHNRVMVLE
HHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VMGRHTGWIALHAGLAGGADVILIPEIPFSIERVAEKVMARDQQGSSFSIIVVAEGARPR
ECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
GGSEMYIAEGRLGGIGHWVGEQLEKLTAKEVRVVVLGHLQRGGSPSPYDRLLSTRYGAAA
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCCHHH
VQAAARGIYGEMVALRGQDIVTVPLAEACGHLNRVRPHSDLVLCARSLGIAFGDEL
HHHHHHHHHHHHHEECCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASKKQRIGVLTSGGDAPGLNAVIRAVVKSASGLGWEVIGIHDGFEGLLGTKSYRVLTNA
CCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHCCCCEEEEECC
DVQGLLPRGGTILRTTNKGHFGPRRSDELSEADPYVRAVKAIEEMGLRALITIGGEGTQR
CCEEECCCCCEEEEECCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCEEEEEECCCCCHH
IALELHKLGAPVIGVPKTIDNDLAGTDRTFGFDTALQVATDAIDRLHTTAASHNRVMVLE
HHHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VMGRHTGWIALHAGLAGGADVILIPEIPFSIERVAEKVMARDQQGSSFSIIVVAEGARPR
ECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCC
GGSEMYIAEGRLGGIGHWVGEQLEKLTAKEVRVVVLGHLQRGGSPSPYDRLLSTRYGAAA
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCHHHHHHHHCCCHHH
VQAAARGIYGEMVALRGQDIVTVPLAEACGHLNRVRPHSDLVLCARSLGIAFGDEL
HHHHHHHHHHHHHEECCCCEEEEEHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA