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The map label for this gene is yfiU [H]

Identifier: 222523409

GI number: 222523409

Start: 140138

End: 141514

Strand: Direct

Name: yfiU [H]

Synonym: Chy400_0111

Alternate gene names: 222523409

Gene position: 140138-141514 (Clockwise)

Preceding gene: 222523408

Following gene: 222523410

Centisome position: 2.66

GC content: 59.84

Gene sequence:

>1377_bases
ATGGCTTCAGTGGCTGTGCCTGAAACTGATACCCGCCGTGCCATTATGACGGTGCTGTTTGTAGGCGTCTTTATGTCAGC
GCTCGACTCGGCGATTATCGGGCCGATAGTGCCGGCGTTGCGAGCAGCATTCGCAATTGACAATACCCAGGTTGTGCTGG
TCACCATTATTTTTACGCTCTGCTCACTCAGTAGCACGACGTTAATGGCAAGTCTGAGTGATCGCTACGGACGCCGCAAT
GTCTATCTGTTGAATGTGTTTGGGTTTGCCCTCGGTTCTCTGATCATCGCCCTCTCCCACGACCTGACGATGGTGCTGAT
TGGGCGGGCATTGCAGGGTGTCTGTGCCGGTGGGATCACGCCCACTGCCAGTGCCGTGATCGGTGACGTGTTACCGTCGG
CGGAACGGGCGAAGGCGCTGGGGCTGATCGGCGCCACGTCGGGCATGGCCTTCCTGGTCGGCCCGGTACTGGCGTCGCTC
ATCCTGGCGGTGGCCGCCTGGCAATGGATCTTTCTGCTCAATCTACCGGTTGCGGCTGCGGTTATCTTTTTGGGCTGGCG
CGCGCTCCCCCGTGCGACTGCCGTGCACCGGGCAACTGCCGCCACTGCCTTTGACTGGCCGGGACTGACGCTGCTGGTGC
TGATTCTGGTCAGCATCACTCTGGGAATTAATCAGCTTCTTGATCGCTTGCTGGGGTTGACGCTCTGGCCGTGGCTCTTC
CTGCTGGTGGCTCTGCTCACCCCACTGCTGGCATGGCGTGAGGTGCGCGCCCCGGTTCCGTTGCTTCCACCGCGTCTGTT
TACCAATCGCCAGTTGCAGATTGTCTACCTGCTGGCTGCCGGTTCCGGGATTGCCATGGGGAGCATTATCTTTATCACCT
CGGTAGCGGTCAACTTTGGCGTGCCAATCAGTCAGGCCGGCTTCTTCTTGCTACCGCTGGTCTTTCTGGCTTCGGTAACG
TCGATCATCGCCGGACGCATCCTGCCACGGATCGGAGGTCGGATGGCGATGCTGATCGGTTACGGCCAACTGATGATTGG
GACGGGGTTGCTTGGTCTGCCCGCCGCCCCCTTCTGGCTTTTTATTCTGGCAACGCTGATTATGGGGAGCGGCCTGGGTA
TCGTGGTTGGTGGCACCCTGCGCGCTCTGGTGCTTGAAGAGGTCGCCGCCGGTGATCGCGGTGTGGCTCAGAGCGTCATT
AACATCGCGATCAGCATCGGTACCCTGATCGCCGTTGCTCTGATGGCCAGCATCGCCGACGCCTTTGATCTCTCTACCGC
ATACCTGGCCTGCACCGGCGCCATGGCGTTGATGACCGCCATCAGTCTGGCCCTGCGCCGTCAATTCAGTCCACCATCCA
CAGCCAATGCATCATAA

Upstream 100 bases:

>100_bases
TCGGCAGACCCAATTACACGTGATTGAGTAACCTGACACCGCCCGCTTAACCGGCAACTGTCAGATCATAACAGCATTGA
CCAGCACAAAAGGAGGTCTC

Downstream 100 bases:

>100_bases
CTACCAACTTGTGCTATCCTATCCAGCAACGGCAATTGGCCGGGTAGGATAGGCATTGACCATGCGGGTCGCTCTTGTTC
ACGACTATCTCAATCAGTAC

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MASVAVPETDTRRAIMTVLFVGVFMSALDSAIIGPIVPALRAAFAIDNTQVVLVTIIFTLCSLSSTTLMASLSDRYGRRN
VYLLNVFGFALGSLIIALSHDLTMVLIGRALQGVCAGGITPTASAVIGDVLPSAERAKALGLIGATSGMAFLVGPVLASL
ILAVAAWQWIFLLNLPVAAAVIFLGWRALPRATAVHRATAATAFDWPGLTLLVLILVSITLGINQLLDRLLGLTLWPWLF
LLVALLTPLLAWREVRAPVPLLPPRLFTNRQLQIVYLLAAGSGIAMGSIIFITSVAVNFGVPISQAGFFLLPLVFLASVT
SIIAGRILPRIGGRMAMLIGYGQLMIGTGLLGLPAAPFWLFILATLIMGSGLGIVVGGTLRALVLEEVAAGDRGVAQSVI
NIAISIGTLIAVALMASIADAFDLSTAYLACTGAMALMTAISLALRRQFSPPSTANAS

Sequences:

>Translated_458_residues
MASVAVPETDTRRAIMTVLFVGVFMSALDSAIIGPIVPALRAAFAIDNTQVVLVTIIFTLCSLSSTTLMASLSDRYGRRN
VYLLNVFGFALGSLIIALSHDLTMVLIGRALQGVCAGGITPTASAVIGDVLPSAERAKALGLIGATSGMAFLVGPVLASL
ILAVAAWQWIFLLNLPVAAAVIFLGWRALPRATAVHRATAATAFDWPGLTLLVLILVSITLGINQLLDRLLGLTLWPWLF
LLVALLTPLLAWREVRAPVPLLPPRLFTNRQLQIVYLLAAGSGIAMGSIIFITSVAVNFGVPISQAGFFLLPLVFLASVT
SIIAGRILPRIGGRMAMLIGYGQLMIGTGLLGLPAAPFWLFILATLIMGSGLGIVVGGTLRALVLEEVAAGDRGVAQSVI
NIAISIGTLIAVALMASIADAFDLSTAYLACTGAMALMTAISLALRRQFSPPSTANAS
>Mature_457_residues
ASVAVPETDTRRAIMTVLFVGVFMSALDSAIIGPIVPALRAAFAIDNTQVVLVTIIFTLCSLSSTTLMASLSDRYGRRNV
YLLNVFGFALGSLIIALSHDLTMVLIGRALQGVCAGGITPTASAVIGDVLPSAERAKALGLIGATSGMAFLVGPVLASLI
LAVAAWQWIFLLNLPVAAAVIFLGWRALPRATAVHRATAATAFDWPGLTLLVLILVSITLGINQLLDRLLGLTLWPWLFL
LVALLTPLLAWREVRAPVPLLPPRLFTNRQLQIVYLLAAGSGIAMGSIIFITSVAVNFGVPISQAGFFLLPLVFLASVTS
IIAGRILPRIGGRMAMLIGYGQLMIGTGLLGLPAAPFWLFILATLIMGSGLGIVVGGTLRALVLEEVAAGDRGVAQSVIN
IAISIGTLIAVALMASIADAFDLSTAYLACTGAMALMTAISLALRRQFSPPSTANAS

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=411, Percent_Identity=25.7907542579075, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789042, Length=179, Percent_Identity=29.608938547486, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081983, Length=383, Percent_Identity=25.5874673629243, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788392, Length=426, Percent_Identity=26.9953051643192, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788710, Length=272, Percent_Identity=28.6764705882353, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323735, Length=163, Percent_Identity=28.2208588957055, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47821; Mature: 47690

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVAVPETDTRRAIMTVLFVGVFMSALDSAIIGPIVPALRAAFAIDNTQVVLVTIIFTL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CSLSSTTLMASLSDRYGRRNVYLLNVFGFALGSLIIALSHDLTMVLIGRALQGVCAGGIT
HHCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
PTASAVIGDVLPSAERAKALGLIGATSGMAFLVGPVLASLILAVAAWQWIFLLNLPVAAA
CHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFLGWRALPRATAVHRATAATAFDWPGLTLLVLILVSITLGINQLLDRLLGLTLWPWLF
HHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVALLTPLLAWREVRAPVPLLPPRLFTNRQLQIVYLLAAGSGIAMGSIIFITSVAVNFG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCC
VPISQAGFFLLPLVFLASVTSIIAGRILPRIGGRMAMLIGYGQLMIGTGLLGLPAAPFWL
CCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCHHHHH
FILATLIMGSGLGIVVGGTLRALVLEEVAAGDRGVAQSVINIAISIGTLIAVALMASIAD
HHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDLSTAYLACTGAMALMTAISLALRRQFSPPSTANAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ASVAVPETDTRRAIMTVLFVGVFMSALDSAIIGPIVPALRAAFAIDNTQVVLVTIIFTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CSLSSTTLMASLSDRYGRRNVYLLNVFGFALGSLIIALSHDLTMVLIGRALQGVCAGGIT
HHCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
PTASAVIGDVLPSAERAKALGLIGATSGMAFLVGPVLASLILAVAAWQWIFLLNLPVAAA
CHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFLGWRALPRATAVHRATAATAFDWPGLTLLVLILVSITLGINQLLDRLLGLTLWPWLF
HHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVALLTPLLAWREVRAPVPLLPPRLFTNRQLQIVYLLAAGSGIAMGSIIFITSVAVNFG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCC
VPISQAGFFLLPLVFLASVTSIIAGRILPRIGGRMAMLIGYGQLMIGTGLLGLPAAPFWL
CCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCHHHHH
FILATLIMGSGLGIVVGGTLRALVLEEVAAGDRGVAQSVINIAISIGTLIAVALMASIAD
HHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFDLSTAYLACTGAMALMTAISLALRRQFSPPSTANAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]