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The map label for this gene is leuB [H]

Identifier: 222523346

GI number: 222523346

Start: 49402

End: 50478

Strand: Direct

Name: leuB [H]

Synonym: Chy400_0047

Alternate gene names: 222523346

Gene position: 49402-50478 (Clockwise)

Preceding gene: 222523344

Following gene: 222523347

Centisome position: 0.94

GC content: 58.31

Gene sequence:

>1077_bases
GTGCAAGCTGTGATTGCTGTCCTTCCCGGCGATGGAATCGGGCCAGAGGTTGTGGCTGAGGGCGTGAAGGTACTACAGGC
GATTGCCAGGCGGTTCGGTCATCAATTCACCTTTCACGAGGCTCTGATCGGTGGGTGCGCGATTGATGCGACAGGAGAGC
CACTACCAGCGGCTACGATTACATTATGTCAATCCGCCGATGCGGTGCTGCTCGGTGCAGTAGGCGGGCCGAAATGGGAC
GATCCGCAAGCCCGTGTGCGCCCAGAGCAGGGATTGTTGGGCATTCGGAAAGCACTCGGCCTGTTTGCCAACCTTCGTCC
GGTACGCATCTATCCGCAGTTGATCAACGCCTCGCCGATCCGACCTGAACGGTTGCAGGGAGTTGATCTGATTGTCGTCC
GTGAACTGACCGGCGGTATCTACTTCGGCGAGAAACGTCGCGAACGCACTGCCGATGGCGAATGGGCCAGCGACCTCTGC
ATCTACACCGAGGAAGAGATTGTGCGCGTTGTGCGCGTTGCAGCACGGCTGGCCCGTCAACGACGCGGTAAAGTTACTCT
GGTTGATAAGGCCAACGTTTTGGAGACGAGCCGTCTATGGCGGAGTATCACCACACGTCTGATGCAAACCGAGTTCCCCG
ATCTGCAATTTGAGACCATGCTGGTTGATGCGTGTGCTATGCACCTCATCCGCCGTCCGGCTGATTTCGATGTGATCGTC
ACCGAGAATATGTTTGGCGACATCCTTACCGATGAAGCCTCGATGCTGGCCGGTTCGATGGGTATGCTCCCATCAGCGTC
ACTGGCTGATGGTCGGCTGGGTTTGTACGAACCGATCCACGGCTCGGCACCGGACATTGCCGGTCAAGGCAAGGCGAATC
CACTGGCAACGATCCTGAGTGTAGCGATGCTCTTGCGCTACTCGCTCGATCTACCCGCCGAAGCCGATTGTGTCGAAGCG
GCAGTTGCCTCGGTGATCGAACGGGGAATTGTGACCGCCGATATTGCTGTGCCGGGACAGGCTGCGTACAGTACCGCAGC
CGTCGGTGATGCGGTAGTTGCTGCGATTGCCGCTTGA

Upstream 100 bases:

>100_bases
CGTGTTACCATCTATGACAGGCGTTCGCACACTTAGTAGTATCATACATGCGTGTCCTTACCGTTTCATAACCATTTTTG
AATGTGTTGCGAGGTTTACT

Downstream 100 bases:

>100_bases
CTGGCCGGCTATGCTATACTGAAGGCCACGCTACCGAACCAGAATCGTGAACAGCGGAAGGAGCGCTCGATGGAGCGAAA
GATTCGTGTGTTGGTCGCCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MQAVIAVLPGDGIGPEVVAEGVKVLQAIARRFGHQFTFHEALIGGCAIDATGEPLPAATITLCQSADAVLLGAVGGPKWD
DPQARVRPEQGLLGIRKALGLFANLRPVRIYPQLINASPIRPERLQGVDLIVVRELTGGIYFGEKRRERTADGEWASDLC
IYTEEEIVRVVRVAARLARQRRGKVTLVDKANVLETSRLWRSITTRLMQTEFPDLQFETMLVDACAMHLIRRPADFDVIV
TENMFGDILTDEASMLAGSMGMLPSASLADGRLGLYEPIHGSAPDIAGQGKANPLATILSVAMLLRYSLDLPAEADCVEA
AVASVIERGIVTADIAVPGQAAYSTAAVGDAVVAAIAA

Sequences:

>Translated_358_residues
MQAVIAVLPGDGIGPEVVAEGVKVLQAIARRFGHQFTFHEALIGGCAIDATGEPLPAATITLCQSADAVLLGAVGGPKWD
DPQARVRPEQGLLGIRKALGLFANLRPVRIYPQLINASPIRPERLQGVDLIVVRELTGGIYFGEKRRERTADGEWASDLC
IYTEEEIVRVVRVAARLARQRRGKVTLVDKANVLETSRLWRSITTRLMQTEFPDLQFETMLVDACAMHLIRRPADFDVIV
TENMFGDILTDEASMLAGSMGMLPSASLADGRLGLYEPIHGSAPDIAGQGKANPLATILSVAMLLRYSLDLPAEADCVEA
AVASVIERGIVTADIAVPGQAAYSTAAVGDAVVAAIAA
>Mature_358_residues
MQAVIAVLPGDGIGPEVVAEGVKVLQAIARRFGHQFTFHEALIGGCAIDATGEPLPAATITLCQSADAVLLGAVGGPKWD
DPQARVRPEQGLLGIRKALGLFANLRPVRIYPQLINASPIRPERLQGVDLIVVRELTGGIYFGEKRRERTADGEWASDLC
IYTEEEIVRVVRVAARLARQRRGKVTLVDKANVLETSRLWRSITTRLMQTEFPDLQFETMLVDACAMHLIRRPADFDVIV
TENMFGDILTDEASMLAGSMGMLPSASLADGRLGLYEPIHGSAPDIAGQGKANPLATILSVAMLLRYSLDLPAEADCVEA
AVASVIERGIVTADIAVPGQAAYSTAAVGDAVVAAIAA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=329, Percent_Identity=31.6109422492401, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=27.6162790697674, Blast_Score=109, Evalue=4e-24,
Organism=Homo sapiens, GI28178816, Length=310, Percent_Identity=29.0322580645161, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI28178821, Length=321, Percent_Identity=28.6604361370716, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI28178838, Length=319, Percent_Identity=26.9592476489028, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI28178819, Length=154, Percent_Identity=33.1168831168831, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=51.123595505618, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788101, Length=362, Percent_Identity=33.9779005524862, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=27, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71986051, Length=354, Percent_Identity=31.9209039548023, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=352, Percent_Identity=28.125, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17505779, Length=342, Percent_Identity=30.9941520467836, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=357, Percent_Identity=29.1316526610644, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=359, Percent_Identity=50.4178272980501, Blast_Score=324, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6322097, Length=370, Percent_Identity=34.5945945945946, Blast_Score=172, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=241, Percent_Identity=35.2697095435685, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=28.0701754385965, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643270, Length=340, Percent_Identity=33.8235294117647, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24643268, Length=340, Percent_Identity=33.8235294117647, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24661184, Length=354, Percent_Identity=27.683615819209, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281362242, Length=338, Percent_Identity=28.698224852071, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24648872, Length=338, Percent_Identity=28.698224852071, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI161078639, Length=248, Percent_Identity=27.8225806451613, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI161078635, Length=338, Percent_Identity=26.6272189349112, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=26.6272189349112, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=26.5486725663717, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=26.5486725663717, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI20130355, Length=180, Percent_Identity=27.2222222222222, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38200; Mature: 38200

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVIAVLPGDGIGPEVVAEGVKVLQAIARRFGHQFTFHEALIGGCAIDATGEPLPAATI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEECCCCCCCCHHHH
TLCQSADAVLLGAVGGPKWDDPQARVRPEQGLLGIRKALGLFANLRPVRIYPQLINASPI
HHHCCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCC
RPERLQGVDLIVVRELTGGIYFGEKRRERTADGEWASDLCIYTEEEIVRVVRVAARLARQ
CHHHHCCCCEEEEEECCCCEEECHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
RRGKVTLVDKANVLETSRLWRSITTRLMQTEFPDLQFETMLVDACAMHLIRRPADFDVIV
HCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TENMFGDILTDEASMLAGSMGMLPSASLADGRLGLYEPIHGSAPDIAGQGKANPLATILS
ECCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VAMLLRYSLDLPAEADCVEAAVASVIERGIVTADIAVPGQAAYSTAAVGDAVVAAIAA
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQAVIAVLPGDGIGPEVVAEGVKVLQAIARRFGHQFTFHEALIGGCAIDATGEPLPAATI
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCEEECCCCCCCCHHHH
TLCQSADAVLLGAVGGPKWDDPQARVRPEQGLLGIRKALGLFANLRPVRIYPQLINASPI
HHHCCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHCCCCC
RPERLQGVDLIVVRELTGGIYFGEKRRERTADGEWASDLCIYTEEEIVRVVRVAARLARQ
CHHHHCCCCEEEEEECCCCEEECHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
RRGKVTLVDKANVLETSRLWRSITTRLMQTEFPDLQFETMLVDACAMHLIRRPADFDVIV
HCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCEEEE
TENMFGDILTDEASMLAGSMGMLPSASLADGRLGLYEPIHGSAPDIAGQGKANPLATILS
ECCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VAMLLRYSLDLPAEADCVEAAVASVIERGIVTADIAVPGQAAYSTAAVGDAVVAAIAA
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA