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The map label for this gene is hyuB [H]

Identifier: 222102423

GI number: 222102423

Start: 17011

End: 18726

Strand: Direct

Name: hyuB [H]

Synonym: Avi_7017

Alternate gene names: 222102423

Gene position: 17011-18726 (Clockwise)

Preceding gene: 222102422

Following gene: 222102424

Centisome position: 2.69

GC content: 58.86

Gene sequence:

>1716_bases
ATGAAGCGGAAAGATCCAGTCCTTCACTTGGCAGGGGTTCCCAGCAAGGGGCTTGCCGTCAACTCCGATCCAATCACGAC
GGAAGTCGTGCGGCATGCGCTCAATTCGGCCGCCAACCAGATGAAGCGGGCGCTGATCCGTACGGCTTTCTCGCCGATCA
TTTACGAGGTGCTGGACTTTGCTGTCGCCATCTATGATCGCGAGCTGCGCATGCTCTCTCAAGCGCCAAGCCTTCCCATC
TTCATGGGAACGCTGAACTTTGCCGTGCGCGAGGCTGTCAAGAATATCGGCGGCGAAGACAAGATCTTCGATGGCGACAT
CCTGATGTACAATTGGCCTTATGGCACTGGTTCGCATCCCCAGGACATGGTCATCATCATGCCGGTCTTCTATCGGCAGG
ACCTGATTGGGTACACGGCGATCAAGGGCCACTGGCTCGATATCGCCGCCAAGGATCCCTATTGCACCGACACCACGGAT
GTCTTCCAGGAAGGCACGATCTTCCCGGGCGTGAAGATCTACAAAAAGGGCGAGCTTAATGACGACATCTATCGCATGAT
CCTCGGCAACAGCCGCGTTCCCAAGGCCGTATCGGGTGACCTCGATGCCCAGGTTACCGGATGCCGCGTCGGAGCCAAGA
GCTTCCTCGAAGTAGTGGAGCGTTTCGGCCTCGACACGTATCGCAATGCTGTCGAGCATATGTTCGACCATGGAGAGGCC
ATCATTCGCAGCTTCTTCGAGAAGATTCCCGATGGCCGCTATGTGGGCAATGGGGAAATGGACGACAACGGCGTCACCAA
GAACCGAATTCCCTTTGAAATCACTGTCGACGTGAAGGGCTCGGACGTCACCATCGACTTTTCCGAAACCCCTGATGCGC
AGGGCGGTCCCGTCAACAGCCCCCTGCCATCGACGATCTCCGCGAGCCGTGTTGCGATTACCATGCTTGCCGGCTATGGC
GAAGCGCCCCATGAAGGCCACTTCCGGCCTATCAAGGTCATCACCCGCCCCGGCAGTATGTTCGATCCCGAACCGCCGTC
TCCCTGCTTTCTCTATGGCTGGTCGGCTTTGCAGGCAATCGAAGTCATCTACAACGCTATCTCCAAGGCGCTGCCTTCAT
CTGTTCCGGCCTGCTCTGGCGGGTGTATTTGCTCGGTCGTCTGGTGGGGAACCCGCGAGGAAACCGGCGAGGCCTGGGCG
GATGGTGCCCCGCATCCGACCGGGCAGGGTGCCTGGGATGGCGGCGATGGCGGCACCATGCTGCATATCTCGGAATCGGC
CACGCGCTTCACCCCGGTCGAAGTATGGGAGGCCCGCAATCCTTTCCTCGTCGAGCGCCTTGCTCTGGCGCAGGATTCCG
TCGGTCCCGGCAAATATCGTGGCGGACCAGGCATCGATCTCTTCTTTGAGATGACGGAAGACACATTCATCACAACGGTG
TTCGAACGCTCGAAGAGCCAGCCCTGGGGACTTGAGGGCGGCCTTGGTGGCCGTGCGAACAATGCAATGGTGGTCATGCC
GGACGGCACAGAACATATCGTTCCGAAAACCACGCATTTTCTCGTCCCTAAGGGCGGCTTGCTCAAGCTGCAAACCGGCG
GCGGCGGCGGATATGGCGAGCCCGCTGAGCGACCTCTGGAGAAGATCCAGGCCGATCTCGATGACGGTTATATCTCTGAG
GCCTTTGCGCGACAGCATTATCCGCACGCATTCTGA

Upstream 100 bases:

>100_bases
CCATCATCACCGAAGGAACCTGTACGACCTATCTGGACGCCGATTGGACGGCGCGGATCGGGACTGTCGGCGAAATCATT
CTGGAACGGAAGAATTAATC

Downstream 100 bases:

>100_bases
ATCTCATGAGCCGGAAGGGATACGATTTCTCCCGGTTCTTTTCGCAAAGGAATATTATGCGGTCCTTCTTCGGTCAAATA
TTCAGGGACAGTGAAATGGC

Product: hydantoin utilization protein

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKRKDPVLHLAGVPSKGLAVNSDPITTEVVRHALNSAANQMKRALIRTAFSPIIYEVLDFAVAIYDRELRMLSQAPSLPI
FMGTLNFAVREAVKNIGGEDKIFDGDILMYNWPYGTGSHPQDMVIIMPVFYRQDLIGYTAIKGHWLDIAAKDPYCTDTTD
VFQEGTIFPGVKIYKKGELNDDIYRMILGNSRVPKAVSGDLDAQVTGCRVGAKSFLEVVERFGLDTYRNAVEHMFDHGEA
IIRSFFEKIPDGRYVGNGEMDDNGVTKNRIPFEITVDVKGSDVTIDFSETPDAQGGPVNSPLPSTISASRVAITMLAGYG
EAPHEGHFRPIKVITRPGSMFDPEPPSPCFLYGWSALQAIEVIYNAISKALPSSVPACSGGCICSVVWWGTREETGEAWA
DGAPHPTGQGAWDGGDGGTMLHISESATRFTPVEVWEARNPFLVERLALAQDSVGPGKYRGGPGIDLFFEMTEDTFITTV
FERSKSQPWGLEGGLGGRANNAMVVMPDGTEHIVPKTTHFLVPKGGLLKLQTGGGGGYGEPAERPLEKIQADLDDGYISE
AFARQHYPHAF

Sequences:

>Translated_571_residues
MKRKDPVLHLAGVPSKGLAVNSDPITTEVVRHALNSAANQMKRALIRTAFSPIIYEVLDFAVAIYDRELRMLSQAPSLPI
FMGTLNFAVREAVKNIGGEDKIFDGDILMYNWPYGTGSHPQDMVIIMPVFYRQDLIGYTAIKGHWLDIAAKDPYCTDTTD
VFQEGTIFPGVKIYKKGELNDDIYRMILGNSRVPKAVSGDLDAQVTGCRVGAKSFLEVVERFGLDTYRNAVEHMFDHGEA
IIRSFFEKIPDGRYVGNGEMDDNGVTKNRIPFEITVDVKGSDVTIDFSETPDAQGGPVNSPLPSTISASRVAITMLAGYG
EAPHEGHFRPIKVITRPGSMFDPEPPSPCFLYGWSALQAIEVIYNAISKALPSSVPACSGGCICSVVWWGTREETGEAWA
DGAPHPTGQGAWDGGDGGTMLHISESATRFTPVEVWEARNPFLVERLALAQDSVGPGKYRGGPGIDLFFEMTEDTFITTV
FERSKSQPWGLEGGLGGRANNAMVVMPDGTEHIVPKTTHFLVPKGGLLKLQTGGGGGYGEPAERPLEKIQADLDDGYISE
AFARQHYPHAF
>Mature_571_residues
MKRKDPVLHLAGVPSKGLAVNSDPITTEVVRHALNSAANQMKRALIRTAFSPIIYEVLDFAVAIYDRELRMLSQAPSLPI
FMGTLNFAVREAVKNIGGEDKIFDGDILMYNWPYGTGSHPQDMVIIMPVFYRQDLIGYTAIKGHWLDIAAKDPYCTDTTD
VFQEGTIFPGVKIYKKGELNDDIYRMILGNSRVPKAVSGDLDAQVTGCRVGAKSFLEVVERFGLDTYRNAVEHMFDHGEA
IIRSFFEKIPDGRYVGNGEMDDNGVTKNRIPFEITVDVKGSDVTIDFSETPDAQGGPVNSPLPSTISASRVAITMLAGYG
EAPHEGHFRPIKVITRPGSMFDPEPPSPCFLYGWSALQAIEVIYNAISKALPSSVPACSGGCICSVVWWGTREETGEAWA
DGAPHPTGQGAWDGGDGGTMLHISESATRFTPVEVWEARNPFLVERLALAQDSVGPGKYRGGPGIDLFFEMTEDTFITTV
FERSKSQPWGLEGGLGGRANNAMVVMPDGTEHIVPKTTHFLVPKGGLLKLQTGGGGGYGEPAERPLEKIQADLDDGYISE
AFARQHYPHAF

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=547, Percent_Identity=28.8848263254113, Blast_Score=177, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI133901900, Length=561, Percent_Identity=26.7379679144385, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI133901902, Length=533, Percent_Identity=25.891181988743, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6322634, Length=560, Percent_Identity=24.6428571428571, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550492, Length=548, Percent_Identity=27.1897810218978, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 62298; Mature: 62298

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKDPVLHLAGVPSKGLAVNSDPITTEVVRHALNSAANQMKRALIRTAFSPIIYEVLDF
CCCCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAIYDRELRMLSQAPSLPIFMGTLNFAVREAVKNIGGEDKIFDGDILMYNWPYGTGSHP
HHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCEECCCEEEEECCCCCCCCC
QDMVIIMPVFYRQDLIGYTAIKGHWLDIAAKDPYCTDTTDVFQEGTIFPGVKIYKKGELN
CCEEEEEEHHHHHHHHCEEEECCEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEECCCCC
DDIYRMILGNSRVPKAVSGDLDAQVTGCRVGAKSFLEVVERFGLDTYRNAVEHMFDHGEA
HHHHHHHHCCCCCCCHHCCCCCCEEEEEHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IIRSFFEKIPDGRYVGNGEMDDNGVTKNRIPFEITVDVKGSDVTIDFSETPDAQGGPVNS
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCC
PLPSTISASRVAITMLAGYGEAPHEGHFRPIKVITRPGSMFDPEPPSPCFLYGWSALQAI
CCCCCCCHHHEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHH
EVIYNAISKALPSSVPACSGGCICSVVWWGTREETGEAWADGAPHPTGQGAWDGGDGGTM
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCCEE
LHISESATRFTPVEVWEARNPFLVERLALAQDSVGPGKYRGGPGIDLFFEMTEDTFITTV
EEEECCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHH
FERSKSQPWGLEGGLGGRANNAMVVMPDGTEHIVPKTTHFLVPKGGLLKLQTGGGGGYGE
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCC
PAERPLEKIQADLDDGYISEAFARQHYPHAF
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKRKDPVLHLAGVPSKGLAVNSDPITTEVVRHALNSAANQMKRALIRTAFSPIIYEVLDF
CCCCCCEEEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVAIYDRELRMLSQAPSLPIFMGTLNFAVREAVKNIGGEDKIFDGDILMYNWPYGTGSHP
HHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCCEECCCEEEEECCCCCCCCC
QDMVIIMPVFYRQDLIGYTAIKGHWLDIAAKDPYCTDTTDVFQEGTIFPGVKIYKKGELN
CCEEEEEEHHHHHHHHCEEEECCEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEECCCCC
DDIYRMILGNSRVPKAVSGDLDAQVTGCRVGAKSFLEVVERFGLDTYRNAVEHMFDHGEA
HHHHHHHHCCCCCCCHHCCCCCCEEEEEHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IIRSFFEKIPDGRYVGNGEMDDNGVTKNRIPFEITVDVKGSDVTIDFSETPDAQGGPVNS
HHHHHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCC
PLPSTISASRVAITMLAGYGEAPHEGHFRPIKVITRPGSMFDPEPPSPCFLYGWSALQAI
CCCCCCCHHHEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCEEEECHHHHHHH
EVIYNAISKALPSSVPACSGGCICSVVWWGTREETGEAWADGAPHPTGQGAWDGGDGGTM
HHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCCCCCCCCCCCCCEE
LHISESATRFTPVEVWEARNPFLVERLALAQDSVGPGKYRGGPGIDLFFEMTEDTFITTV
EEEECCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHH
FERSKSQPWGLEGGLGGRANNAMVVMPDGTEHIVPKTTHFLVPKGGLLKLQTGGGGGYGE
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCC
PAERPLEKIQADLDDGYISEAFARQHYPHAF
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]