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The map label for this gene is aph [H]

Identifier: 222095113

GI number: 222095113

Start: 1431217

End: 1432119

Strand: Reverse

Name: aph [H]

Synonym: BCQ_1453

Alternate gene names: 222095113

Gene position: 1432119-1431217 (Counterclockwise)

Preceding gene: 222095115

Following gene: 222095107

Centisome position: 27.47

GC content: 33.55

Gene sequence:

>903_bases
ATGGACTCTTACAAACAATATATAAAAGAAGCTTTGCCTAATCTTTCTATACATTCCTATAAACAAAATGAAGAAGGATG
GGATAATGTAGCGATTATAGTAAATGATGAGCTACTGTTTCGTTTCCCGCGAAAACAGGAATATGCAATGCGAATTCCGT
TAGAAAAAGAACTGTGCACAATTCTCACTCAATCACTACAAGAAATCGAAGTTCCACAATATCACCTAATTTATACAAAT
GAATCTGATGAGGTTCCTCTTTGTAGCTACTATACTCTCATTCATGGTGAACCGTTAAAAACAGAAATAGTTGCGAACTT
AGATGAAAAAGAACGAAAAATAATTATTACACAATTAGCTACTTTCCTTGCGGCTCTACATAGCATTCCTCTAAAAAGTG
TTACAGCGTTAGGGTTCCCTACCGAAAAAACACTTACCTACTGGAAAGAGCTACAAACTAAATTAAATGAGTATGTTACT
AACAGTCTAACTTCGTTCCAGAAATCAACCTTAAACCGTTTATTCGAGAATTTCTTTGCCTGTCTAGCTACATCCAAATT
TCAAAATACAATCATTCACGCTGATTTTACACATCATCACATTTTATTTGATAAACAAAATAAAACCATTTCAGGAATCA
TTGATTTTGGTGATGCTCAAATTGGAGATCCTGCCTTTGACTTTGCTGGGCTATATGATGATTTCGGACATGAATTTACT
ACATCAGTATATGAACAATACTGTACACTTACTTCACACCATGATCCATTACTCATTCACCGCATCACGAGTTTTTATCA
ATACAGTCCTTTACTACATAATATAATTTATAACTTTGAAACAAAGAATGAACTTGAATTTCTAGCAAGTATAGAACAAC
TGAAAGTAATACTACAGGGATGA

Upstream 100 bases:

>100_bases
TAACACTCCCGCCCCTCTTCATAATGATAATTATATTTTAACATATAGAAAGAAAATTCTGATATCATAAAGTGGAATAA
CTTAGAAACGAGGAACAATT

Downstream 100 bases:

>100_bases
GATTACATTCTCTCCCTATATTCCCTCTCCTCATACATCATTAACTTTTTATCCATTTCAATAATGACTTTATATAGTTG
AAATGCTTGTAAAATTTGCA

Product: aminoglycoside phophotransferase

Products: NA

Alternate protein names: 6'-aminoglycoside N-acetyltransferase; AAC(6'); 2''-aminoglycoside phosphotransferase; APH(2'') [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDSYKQYIKEALPNLSIHSYKQNEEGWDNVAIIVNDELLFRFPRKQEYAMRIPLEKELCTILTQSLQEIEVPQYHLIYTN
ESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFLAALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVT
NSLTSFQKSTLNRLFENFFACLATSKFQNTIIHADFTHHHILFDKQNKTISGIIDFGDAQIGDPAFDFAGLYDDFGHEFT
TSVYEQYCTLTSHHDPLLIHRITSFYQYSPLLHNIIYNFETKNELEFLASIEQLKVILQG

Sequences:

>Translated_300_residues
MDSYKQYIKEALPNLSIHSYKQNEEGWDNVAIIVNDELLFRFPRKQEYAMRIPLEKELCTILTQSLQEIEVPQYHLIYTN
ESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFLAALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVT
NSLTSFQKSTLNRLFENFFACLATSKFQNTIIHADFTHHHILFDKQNKTISGIIDFGDAQIGDPAFDFAGLYDDFGHEFT
TSVYEQYCTLTSHHDPLLIHRITSFYQYSPLLHNIIYNFETKNELEFLASIEQLKVILQG
>Mature_300_residues
MDSYKQYIKEALPNLSIHSYKQNEEGWDNVAIIVNDELLFRFPRKQEYAMRIPLEKELCTILTQSLQEIEVPQYHLIYTN
ESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLATFLAALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVT
NSLTSFQKSTLNRLFENFFACLATSKFQNTIIHADFTHHHILFDKQNKTISGIIDFGDAQIGDPAFDFAGLYDDFGHEFT
TSVYEQYCTLTSHHDPLLIHRITSFYQYSPLLHNIIYNFETKNELEFLASIEQLKVILQG

Specific function: Resistance to gentamicin, tobramycin, and kanamycin. Tobramycin and kanamycin resistance is due to the ACC activity, specified by N-terminal region, and the gentamicin resistance is due to the APH activity encoded by the C-terminal region of the protein [

COG id: COG3173

COG function: function code R; Predicted aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF00583 Acetyltransf_1; PF01636 APH [H]

EC number: NA

Molecular weight: Translated: 34865; Mature: 34865

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSYKQYIKEALPNLSIHSYKQNEEGWDNVAIIVNDELLFRFPRKQEYAMRIPLEKELCT
CCHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECHHEEECCCCCCCEEECCCHHHHHH
ILTQSLQEIEVPQYHLIYTNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHEEHEECCCCCHHHHHHCCCHHHHHHHHHHHH
TFLAALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFA
HHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLATSKFQNTIIHADFTHHHILFDKQNKTISGIIDFGDAQIGDPAFDFAGLYDDFGHEFT
HHHHHHHHCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
TSVYEQYCTLTSHHDPLLIHRITSFYQYSPLLHNIIYNFETKNELEFLASIEQLKVILQG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDSYKQYIKEALPNLSIHSYKQNEEGWDNVAIIVNDELLFRFPRKQEYAMRIPLEKELCT
CCHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECHHEEECCCCCCCEEECCCHHHHHH
ILTQSLQEIEVPQYHLIYTNESDEVPLCSYYTLIHGEPLKTEIVANLDEKERKIIITQLA
HHHHHHHHCCCCCEEEEEECCCCCCCHHHEEHEECCCCCHHHHHHCCCHHHHHHHHHHHH
TFLAALHSIPLKSVTALGFPTEKTLTYWKELQTKLNEYVTNSLTSFQKSTLNRLFENFFA
HHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CLATSKFQNTIIHADFTHHHILFDKQNKTISGIIDFGDAQIGDPAFDFAGLYDDFGHEFT
HHHHHHHHCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
TSVYEQYCTLTSHHDPLLIHRITSFYQYSPLLHNIIYNFETKNELEFLASIEQLKVILQG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3015884; 12663927 [H]