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The map label for this gene is kinD [H]

Identifier: 222095067

GI number: 222095067

Start: 1396703

End: 1398199

Strand: Reverse

Name: kinD [H]

Synonym: BCQ_1407

Alternate gene names: 222095067

Gene position: 1398199-1396703 (Counterclockwise)

Preceding gene: 222095072

Following gene: 222095065

Centisome position: 26.82

GC content: 34.27

Gene sequence:

>1497_bases
TTGAAATTCAAATTCCACAGCATAGTTTTATATATATTGCTTGTTATCCTACCAACAACAGGGATTGGTGCTACATTTTA
TTCATATCATTCGTATAAAATGAAACAAGAAAATAAACAAGTTGCCCATACGGTTCTCTTTTTATATAGAGACTATTTAG
ATCACCACCTTGGCGAAGCAATTTCTGCCCTAGAAATGCTCACAAAGGTCGTAGGAACCGAGACTGGAAATATTAATGGG
ATGAAAGAAATTGTACATGATACAGATGGAAACGATGCACGTTTTTCTGGTCTATATTACGCTACACCAGAAGGTATCAT
TACAATCGCATCGGAAGGTGACCGACCACCTGTTGATGTCTCAGATCGTAAATATATTCAAAACGCATTACAAACTAAGA
AAACAACTGTATCATCCGTCATTACAGATCGTGTTCTTGGCCATCAAGCTATTATGATTGCTTCCCCCATTTTTAATAAA
CAGAAGGAGCTCTCAGGCTTATTGTTAGCAAGTTTACGCTTTGACTACATTTCATCATCTTTAAATGCCATTAAGCCACA
ATACCATTTTGAAGTAACTGATAAGTATGATGTAGTTTTTCTAACCGATGATAATAACGAAACAAGTGATGCACATTCTA
ATATGCTTACTACTCCACTCCAAAGATTAGATTGGAAGGTCTCTGTTTCTCCGTTACCTATTCATCAAAAAACTTTAAAT
CAGTGGGTTGCCGTAGAATGTATAGCTACTTTATTTTTAATGTCAATTTTATTTTTACTCGCTCAATATATGTTGTTAAA
ACGACAAACAAAACTAGAAAGACAACAAAATGAACTACAAAAAATTGAATTAGTTGGAACTTTTGCAGCTAGTACCGCCC
ACGAAATCCGTAATCCACTTACCGGAATTAAAGGGCTCGTCGCTCTATTAAAAGAGAAATATAAAGATGAACAAGATCAG
TTCTATTTTTCCATCATCGAACAAGAAATAGAACGAATTAATGAAATTGTAAGTGAATTTCTTATTCTGGGAAAGCCAAC
TGCAATCATTGAACAAACATATGATGTGAGAACCATTCTAAATGAGGTAGCATTAATTATTCAATCTGAGGCGAATCTAC
ATAATATTGTATTCCATTTACATTTGCCAGACCGTCCTGTTCATATCCGATGCTCGAAAGACCATATGAAACAAGTGGTT
TTAAACATTACAAAAAATGCAATTGAAGCTATGACTTTTGGCGATACACTAACTATTTTCGTAACAAACAATGAAACACA
CGCACAATTGCAAATTATAGATACTGGAAAAGGGATTCCAAAACATATTCAAAAACACCTCTTTCATCCGTTCTTTACTA
ATAAAGATACTGGAACAGGTCTTGGACTTGTCATATGTAAACGAATTATAGAGATGTACAACGGGCATATTTTTATTAAC
AGTATAGAAAACGAAGGAACGACAGTTCATATCGAAATTCCGTTACACATAGTATAA

Upstream 100 bases:

>100_bases
ATCTCTCCCCCAAATATATTATGAATGATTCTTGAATAAAAACATGACAATTTTAACATAGTTTATATATAATGATAATA
AAAAAGAAGGAGTACCAAAT

Downstream 100 bases:

>100_bases
TTATCCTTATCCCTAAAGGGTAATTATACTTATTTCATAAAACCAGCCACAAGCATTCGCCAAAAATTACTTGATTTTCC
TTCATACCCAATTTGAAAAA

Product: sensor histidine kinase; sporulation kinase

Products: NA

Alternate protein names: Sensor histidine kinase D [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MKFKFHSIVLYILLVILPTTGIGATFYSYHSYKMKQENKQVAHTVLFLYRDYLDHHLGEAISALEMLTKVVGTETGNING
MKEIVHDTDGNDARFSGLYYATPEGIITIASEGDRPPVDVSDRKYIQNALQTKKTTVSSVITDRVLGHQAIMIASPIFNK
QKELSGLLLASLRFDYISSSLNAIKPQYHFEVTDKYDVVFLTDDNNETSDAHSNMLTTPLQRLDWKVSVSPLPIHQKTLN
QWVAVECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPLTGIKGLVALLKEKYKDEQDQ
FYFSIIEQEIERINEIVSEFLILGKPTAIIEQTYDVRTILNEVALIIQSEANLHNIVFHLHLPDRPVHIRCSKDHMKQVV
LNITKNAIEAMTFGDTLTIFVTNNETHAQLQIIDTGKGIPKHIQKHLFHPFFTNKDTGTGLGLVICKRIIEMYNGHIFIN
SIENEGTTVHIEIPLHIV

Sequences:

>Translated_498_residues
MKFKFHSIVLYILLVILPTTGIGATFYSYHSYKMKQENKQVAHTVLFLYRDYLDHHLGEAISALEMLTKVVGTETGNING
MKEIVHDTDGNDARFSGLYYATPEGIITIASEGDRPPVDVSDRKYIQNALQTKKTTVSSVITDRVLGHQAIMIASPIFNK
QKELSGLLLASLRFDYISSSLNAIKPQYHFEVTDKYDVVFLTDDNNETSDAHSNMLTTPLQRLDWKVSVSPLPIHQKTLN
QWVAVECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPLTGIKGLVALLKEKYKDEQDQ
FYFSIIEQEIERINEIVSEFLILGKPTAIIEQTYDVRTILNEVALIIQSEANLHNIVFHLHLPDRPVHIRCSKDHMKQVV
LNITKNAIEAMTFGDTLTIFVTNNETHAQLQIIDTGKGIPKHIQKHLFHPFFTNKDTGTGLGLVICKRIIEMYNGHIFIN
SIENEGTTVHIEIPLHIV
>Mature_498_residues
MKFKFHSIVLYILLVILPTTGIGATFYSYHSYKMKQENKQVAHTVLFLYRDYLDHHLGEAISALEMLTKVVGTETGNING
MKEIVHDTDGNDARFSGLYYATPEGIITIASEGDRPPVDVSDRKYIQNALQTKKTTVSSVITDRVLGHQAIMIASPIFNK
QKELSGLLLASLRFDYISSSLNAIKPQYHFEVTDKYDVVFLTDDNNETSDAHSNMLTTPLQRLDWKVSVSPLPIHQKTLN
QWVAVECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPLTGIKGLVALLKEKYKDEQDQ
FYFSIIEQEIERINEIVSEFLILGKPTAIIEQTYDVRTILNEVALIIQSEANLHNIVFHLHLPDRPVHIRCSKDHMKQVV
LNITKNAIEAMTFGDTLTIFVTNNETHAQLQIIDTGKGIPKHIQKHLFHPFFTNKDTGTGLGLVICKRIIEMYNGHIFIN
SIENEGTTVHIEIPLHIV

Specific function: Phosphorylates the sporulation-regulatory protein spo0F and, to a minor extent, is responsible for heterogeneous expression of spo0A during logarithmical growth. Also phosphorylates spo0A under biofilm growth conditions [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=254, Percent_Identity=33.4645669291339, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=30.1724137931034, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=27.0042194092827, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=27.8008298755187, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789808, Length=214, Percent_Identity=27.1028037383178, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789149, Length=226, Percent_Identity=27.4336283185841, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790861, Length=246, Percent_Identity=22.7642276422764, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081816, Length=242, Percent_Identity=23.9669421487603, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=242, Percent_Identity=26.0330578512397, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=24.2677824267782, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790346, Length=249, Percent_Identity=21.6867469879518, Blast_Score=66, Evalue=7e-12,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=23.6607142857143, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=23.7442922374429, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 56580; Mature: 56580

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKFHSIVLYILLVILPTTGIGATFYSYHSYKMKQENKQVAHTVLFLYRDYLDHHLGEA
CCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISALEMLTKVVGTETGNINGMKEIVHDTDGNDARFSGLYYATPEGIITIASEGDRPPVDV
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEECCEEEECCCCEEEEECCCCCCCCCC
SDRKYIQNALQTKKTTVSSVITDRVLGHQAIMIASPIFNKQKELSGLLLASLRFDYISSS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LNAIKPQYHFEVTDKYDVVFLTDDNNETSDAHSNMLTTPLQRLDWKVSVSPLPIHQKTLN
HHHCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHH
QWVAVECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIKGLVALLKEKYKDEQDQFYFSIIEQEIERINEIVSEFLILGKPTAIIEQTYDVRTIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NEVALIIQSEANLHNIVFHLHLPDRPVHIRCSKDHMKQVVLNITKNAIEAMTFGDTLTIF
HHHHHHHCCCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHEECCCEEEEE
VTNNETHAQLQIIDTGKGIPKHIQKHLFHPFFTNKDTGTGLGLVICKRIIEMYNGHIFIN
EECCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
SIENEGTTVHIEIPLHIV
EECCCCCEEEEEEEEEEC
>Mature Secondary Structure
MKFKFHSIVLYILLVILPTTGIGATFYSYHSYKMKQENKQVAHTVLFLYRDYLDHHLGEA
CCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISALEMLTKVVGTETGNINGMKEIVHDTDGNDARFSGLYYATPEGIITIASEGDRPPVDV
HHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCEECCEEEECCCCEEEEECCCCCCCCCC
SDRKYIQNALQTKKTTVSSVITDRVLGHQAIMIASPIFNKQKELSGLLLASLRFDYISSS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LNAIKPQYHFEVTDKYDVVFLTDDNNETSDAHSNMLTTPLQRLDWKVSVSPLPIHQKTLN
HHHCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHH
QWVAVECIATLFLMSILFLLAQYMLLKRQTKLERQQNELQKIELVGTFAASTAHEIRNPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIKGLVALLKEKYKDEQDQFYFSIIEQEIERINEIVSEFLILGKPTAIIEQTYDVRTIL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NEVALIIQSEANLHNIVFHLHLPDRPVHIRCSKDHMKQVVLNITKNAIEAMTFGDTLTIF
HHHHHHHCCCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHEECCCEEEEE
VTNNETHAQLQIIDTGKGIPKHIQKHLFHPFFTNKDTGTGLGLVICKRIIEMYNGHIFIN
EECCCCEEEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
SIENEGTTVHIEIPLHIV
EECCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]