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The map label for this gene is pcrA [H]

Identifier: 222094953

GI number: 222094953

Start: 1280164

End: 1282230

Strand: Reverse

Name: pcrA [H]

Synonym: BCQ_1291

Alternate gene names: 222094953

Gene position: 1282230-1280164 (Counterclockwise)

Preceding gene: 222094954

Following gene: 222094952

Centisome position: 24.59

GC content: 34.59

Gene sequence:

>2067_bases
ATGACACAAGAAAAATTTTCACAAAAATCATTAACGCACGCTGATATTCCACGTGCAACATATCATATTCCGAAAACATC
TACCCATTTAATTATGGAAGAAGATGCAGATGCGGCGTATTTCCGCGCTCTCGAACAACAAAATGTATTTTTAAACGAAA
AACAATTAGAGGCCGTTCGAACGACAGAAGGTCCTGTTCTTACATTAGCTGGTGCCGGAAGCGGGAAAACATCTGTTTTA
ACAACTCGCGTAGGTTATTTAGTAAATGTGAAACAAGTTCATCCGCGAAATATTTTATTGCTTACATTTACACAAAAAGC
GGCTGAAGAAATCCGAAATCGTGTAGCGAATTTACCAGGTATGAATCATGCGGCAAGTAGCTACGTTGTTGCTGGTACGT
TCCACTCTGTCTTTTTAAAATTGCTTCGTAGTCAAGGATATAATCAGCAAATTTTAGCAAACGAAAAACATAAACAAATT
ATGATAAAGAAAATTTTAAAAGAATTGCGTTTAAAAGACGCTTATGATGCTGAAACGATGCTCGCGATGATTTCACTTGA
GAAAAACAAGTTGAATCGTCCGAAAGATGTAAAAGCAAAAACACCCGTTGAACAAGAATTTAAAGAAGTATACGAGCGTT
TTGAAGAAGTGAAACAACGATATAATTATATCGACTTCGATGATATCTTATTAGAAACATATTATATGTTAGAAAATAAC
GCTCCTTTGCTTACCCAATTACAACAACGTTTCCACTATATTGAAGTAGATGAGTTCCAAGATACATCGTATGCTCAATA
TGAAATTGTAAAATTGTTAGCTTCGCCAAGAAATAATTTATTTATCGCAGGTGATGACGATCAAGCTATATACGGTTGGC
GAGGAGCAAGTCACCAAATTATTTTATCTTTTCCAAAAGAATTTGATAACACTACGATTATCGCCCTTAATACGAATTAT
CGATCCAATCCTTTTATTGTCGGACTTGGAAATGAAGTTATCAAACTAAATCAAGAACGGTTTGATAAAGAGCTATATTC
TGTTCGTGAAGAAGGTGTACAACCTTTTTATGCAAGACCGGCTACGACTCTTGATGAAGCAAATCAAATTTTGCAGCTCA
TTCAAGAAAAAGTAGATAGTGGTGAAAGAAATTATAAAGATTTTTGTTTACTATATCGCACACATTCCGTAAGTCGTTCG
TTACTTGATCAGCTTACCATTCATAAAATCCCGTTCATTAAACACGGTGCGAGTCAGTCATTTTATGAACATTCTTTAAT
TAAGCCTGTATTAGATCATTTACGACTCGTAATTGATCCGTTTCGATTGGAATCACTTTCAAATATATTGCCAACGATGT
ATATCGGGCGTGATGATTGTATTTCATTTATTGAACGTGAACAATGGAAATATGGTGAAGGCCGTTTCCCTTCTCTTCTT
CATTTCTTATTATTAAATCCTAGCTTAAAACCTTTTCAAGTGAAAAAGGTAAATGAGCGTATCGATTTTATTAAATTTAT
AAAAGAATTAGAACCGAAAAAAGCTTTAAAAGAAATTATTCATGGTAAAGGAAAGTATTTAGAGTACTTACAAAGTAACG
ATCGTTCTTCCTTTACGATGCATAAAGATATACAAGAAGAAATGTTAGAAGAATTAATGGAGTCAGCAAATCGTTTCACC
GACATTCCAGCTTATTTACAATTTATTGATGAAGCAATTCAAGGGCAAAAAGAAATGGAAGCATTAAAAACAATGCCGCA
AAAAGATGCTGTTTCACTTATGTCTATTCATAACGCAAAGGGCCTTGAATTTCCATGTGTCTTTTTACTGGGAGCAAGCG
ATGGTATTCTGCCCCATACTTCTTCTTTAAAAGATGCAAATGATCGTGTTACAGAGACTTCTGAAGCTTTAGAAGAAGAA
CGCCGACTACTTTATGTCGCAATTACACGCGCAAAAGAAGAACTGTACATTTCCTCTCCGCAATTTTTCAGAGGAAAAAA
ATTAGATATATCTCGTTTTTTGTACACTGTACGAAAGGATTTACCTGAAAAGACATCCACTAAATAA

Upstream 100 bases:

>100_bases
ATTTGAAAACAGGAGGTATCCCCTCCTGTTTTTTACATTGCTATTTTCTTTTTCTTCCGCTAAAGTTAAATGTTGATGCT
ATAGTTTTGAAGGTGAATAT

Downstream 100 bases:

>100_bases
GGATGTCTTTTTTTATATTGCATTATGTACATCCCTCCTATATAAATTCCCTGTTTTCATCATGATAATTAACGTAATAT
CAACCATATCTCATTCATAT

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MTQEKFSQKSLTHADIPRATYHIPKTSTHLIMEEDADAAYFRALEQQNVFLNEKQLEAVRTTEGPVLTLAGAGSGKTSVL
TTRVGYLVNVKQVHPRNILLLTFTQKAAEEIRNRVANLPGMNHAASSYVVAGTFHSVFLKLLRSQGYNQQILANEKHKQI
MIKKILKELRLKDAYDAETMLAMISLEKNKLNRPKDVKAKTPVEQEFKEVYERFEEVKQRYNYIDFDDILLETYYMLENN
APLLTQLQQRFHYIEVDEFQDTSYAQYEIVKLLASPRNNLFIAGDDDQAIYGWRGASHQIILSFPKEFDNTTIIALNTNY
RSNPFIVGLGNEVIKLNQERFDKELYSVREEGVQPFYARPATTLDEANQILQLIQEKVDSGERNYKDFCLLYRTHSVSRS
LLDQLTIHKIPFIKHGASQSFYEHSLIKPVLDHLRLVIDPFRLESLSNILPTMYIGRDDCISFIEREQWKYGEGRFPSLL
HFLLLNPSLKPFQVKKVNERIDFIKFIKELEPKKALKEIIHGKGKYLEYLQSNDRSSFTMHKDIQEEMLEELMESANRFT
DIPAYLQFIDEAIQGQKEMEALKTMPQKDAVSLMSIHNAKGLEFPCVFLLGASDGILPHTSSLKDANDRVTETSEALEEE
RRLLYVAITRAKEELYISSPQFFRGKKLDISRFLYTVRKDLPEKTSTK

Sequences:

>Translated_688_residues
MTQEKFSQKSLTHADIPRATYHIPKTSTHLIMEEDADAAYFRALEQQNVFLNEKQLEAVRTTEGPVLTLAGAGSGKTSVL
TTRVGYLVNVKQVHPRNILLLTFTQKAAEEIRNRVANLPGMNHAASSYVVAGTFHSVFLKLLRSQGYNQQILANEKHKQI
MIKKILKELRLKDAYDAETMLAMISLEKNKLNRPKDVKAKTPVEQEFKEVYERFEEVKQRYNYIDFDDILLETYYMLENN
APLLTQLQQRFHYIEVDEFQDTSYAQYEIVKLLASPRNNLFIAGDDDQAIYGWRGASHQIILSFPKEFDNTTIIALNTNY
RSNPFIVGLGNEVIKLNQERFDKELYSVREEGVQPFYARPATTLDEANQILQLIQEKVDSGERNYKDFCLLYRTHSVSRS
LLDQLTIHKIPFIKHGASQSFYEHSLIKPVLDHLRLVIDPFRLESLSNILPTMYIGRDDCISFIEREQWKYGEGRFPSLL
HFLLLNPSLKPFQVKKVNERIDFIKFIKELEPKKALKEIIHGKGKYLEYLQSNDRSSFTMHKDIQEEMLEELMESANRFT
DIPAYLQFIDEAIQGQKEMEALKTMPQKDAVSLMSIHNAKGLEFPCVFLLGASDGILPHTSSLKDANDRVTETSEALEEE
RRLLYVAITRAKEELYISSPQFFRGKKLDISRFLYTVRKDLPEKTSTK
>Mature_687_residues
TQEKFSQKSLTHADIPRATYHIPKTSTHLIMEEDADAAYFRALEQQNVFLNEKQLEAVRTTEGPVLTLAGAGSGKTSVLT
TRVGYLVNVKQVHPRNILLLTFTQKAAEEIRNRVANLPGMNHAASSYVVAGTFHSVFLKLLRSQGYNQQILANEKHKQIM
IKKILKELRLKDAYDAETMLAMISLEKNKLNRPKDVKAKTPVEQEFKEVYERFEEVKQRYNYIDFDDILLETYYMLENNA
PLLTQLQQRFHYIEVDEFQDTSYAQYEIVKLLASPRNNLFIAGDDDQAIYGWRGASHQIILSFPKEFDNTTIIALNTNYR
SNPFIVGLGNEVIKLNQERFDKELYSVREEGVQPFYARPATTLDEANQILQLIQEKVDSGERNYKDFCLLYRTHSVSRSL
LDQLTIHKIPFIKHGASQSFYEHSLIKPVLDHLRLVIDPFRLESLSNILPTMYIGRDDCISFIEREQWKYGEGRFPSLLH
FLLLNPSLKPFQVKKVNERIDFIKFIKELEPKKALKEIIHGKGKYLEYLQSNDRSSFTMHKDIQEEMLEELMESANRFTD
IPAYLQFIDEAIQGQKEMEALKTMPQKDAVSLMSIHNAKGLEFPCVFLLGASDGILPHTSSLKDANDRVTETSEALEEER
RLLYVAITRAKEELYISSPQFFRGKKLDISRFLYTVRKDLPEKTSTK

Specific function: May be involved in the generation of recombinogenic substrates for the subsequent action of recA [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=675, Percent_Identity=31.7037037037037, Blast_Score=270, Evalue=3e-73,
Organism=Escherichia coli, GI48994965, Length=651, Percent_Identity=29.1858678955453, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1787196, Length=382, Percent_Identity=27.4869109947644, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=410, Percent_Identity=27.8048780487805, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324477, Length=419, Percent_Identity=22.9116945107399, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79613; Mature: 79482

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEKFSQKSLTHADIPRATYHIPKTSTHLIMEEDADAAYFRALEQQNVFLNEKQLEAVR
CCCHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHH
TTEGPVLTLAGAGSGKTSVLTTRVGYLVNVKQVHPRNILLLTFTQKAAEEIRNRVANLPG
CCCCCEEEEEECCCCCCHHHEEHHHHEEEEEEECCCEEEEEEECHHHHHHHHHHHHCCCC
MNHAASSYVVAGTFHSVFLKLLRSQGYNQQILANEKHKQIMIKKILKELRLKDAYDAETM
CCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHH
LAMISLEKNKLNRPKDVKAKTPVEQEFKEVYERFEEVKQRYNYIDFDDILLETYYMLENN
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
APLLTQLQQRFHYIEVDEFQDTSYAQYEIVKLLASPRNNLFIAGDDDQAIYGWRGASHQI
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCE
ILSFPKEFDNTTIIALNTNYRSNPFIVGLGNEVIKLNQERFDKELYSVREEGVQPFYARP
EEECCCCCCCCEEEEEECCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHCCCCEECCC
ATTLDEANQILQLIQEKVDSGERNYKDFCLLYRTHSVSRSLLDQLTIHKIPFIKHGASQS
CCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHCCCEECCCCCH
FYEHSLIKPVLDHLRLVIDPFRLESLSNILPTMYIGRDDCISFIEREQWKYGEGRFPSLL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHH
HFLLLNPSLKPFQVKKVNERIDFIKFIKELEPKKALKEIIHGKGKYLEYLQSNDRSSFTM
HHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCHH
HKDIQEEMLEELMESANRFTDIPAYLQFIDEAIQGQKEMEALKTMPQKDAVSLMSIHNAK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCC
GLEFPCVFLLGASDGILPHTSSLKDANDRVTETSEALEEERRLLYVAITRAKEELYISSP
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCHHHEEECCC
QFFRGKKLDISRFLYTVRKDLPEKTSTK
CHHCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQEKFSQKSLTHADIPRATYHIPKTSTHLIMEEDADAAYFRALEQQNVFLNEKQLEAVR
CCHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHH
TTEGPVLTLAGAGSGKTSVLTTRVGYLVNVKQVHPRNILLLTFTQKAAEEIRNRVANLPG
CCCCCEEEEEECCCCCCHHHEEHHHHEEEEEEECCCEEEEEEECHHHHHHHHHHHHCCCC
MNHAASSYVVAGTFHSVFLKLLRSQGYNQQILANEKHKQIMIKKILKELRLKDAYDAETM
CCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHH
LAMISLEKNKLNRPKDVKAKTPVEQEFKEVYERFEEVKQRYNYIDFDDILLETYYMLENN
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
APLLTQLQQRFHYIEVDEFQDTSYAQYEIVKLLASPRNNLFIAGDDDQAIYGWRGASHQI
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCE
ILSFPKEFDNTTIIALNTNYRSNPFIVGLGNEVIKLNQERFDKELYSVREEGVQPFYARP
EEECCCCCCCCEEEEEECCCCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHCCCCEECCC
ATTLDEANQILQLIQEKVDSGERNYKDFCLLYRTHSVSRSLLDQLTIHKIPFIKHGASQS
CCCHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHHHHHHHHHHHHHHHHHCCCEECCCCCH
FYEHSLIKPVLDHLRLVIDPFRLESLSNILPTMYIGRDDCISFIEREQWKYGEGRFPSLL
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHH
HFLLLNPSLKPFQVKKVNERIDFIKFIKELEPKKALKEIIHGKGKYLEYLQSNDRSSFTM
HHHHHCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCHH
HKDIQEEMLEELMESANRFTDIPAYLQFIDEAIQGQKEMEALKTMPQKDAVSLMSIHNAK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCC
GLEFPCVFLLGASDGILPHTSSLKDANDRVTETSEALEEERRLLYVAITRAKEELYISSP
CCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEECCHHHEEECCC
QFFRGKKLDISRFLYTVRKDLPEKTSTK
CHHCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]