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The map label for this gene is thiF [H]

Identifier: 222094486

GI number: 222094486

Start: 811644

End: 812585

Strand: Direct

Name: thiF [H]

Synonym: BCQ_0824

Alternate gene names: 222094486

Gene position: 811644-812585 (Clockwise)

Preceding gene: 222094485

Following gene: 222094487

Centisome position: 15.57

GC content: 38.0

Gene sequence:

>942_bases
GTGCTCATTATCGGTGCAGGCGCACTAGGTAGTGCAAATGCAGAAATGTTTGTAAGAGCTGGTGTTGGCACAGTAACGAT
TGTTGACCGGGATTATGTCGATTGGAGTAATTTACAAAGGCAGCAATTGTATGCAGAGAGTGATGTGGAAAATAATCTTC
CGAAAGCTGTAGCAGCGAAGAAGCGTCTAGAAGAGATTAATAGTGAAGTAAGAGTAGAAGCGCTCGTTCAAGATGTAACA
GCTGAGGAGTTAGAAGAACTCGTTACAAACGTTGATGTAATGATTGATGCAACTGATAATTTCGAAACGCGTTTCATTGT
GAATGATATGGCACAAAAATATTCTATTCCATGGATTTACGGAGCATGTGTAGGGAGTTACGGTCTTTCTTACACAATCC
TTCCTAGTAAAACGCCATGTTTATCTTGTTTATTACAATCGATTCCGCTTGGCGGAGCGACATGTGATACAGCGGGGATT
ATATCACCTGCTGTATCTCTCGTCGTTTCTCATCAAGTAACGGAAGCTCTTAAACTGTTAGTGGAAGATTACGAATCACT
TCGAGATGGACTTGTATCGTTTGATGTATGGAAGAATGAATATTCATGTATGAATGTGCAAAAGCTTCGTAAACATAATT
GCCCTTCGTGCGGAGAGAATGCATTATATCCTTATTTAAACAAAGAAAATACATCGAAAACAGCAGTTTTATGCGGGAGA
AATACAGTTCAAATTAGACCACCTCATAAAGAGGAAATAGATTTTGAACGATACAAAGAGCTGCTGCATGATCGTGTGAA
TGATTTAAATGTAAATCCATATTTATTATCATTTTCTGTGGAAGAAAAGAGATTAGTTGCTTTTAAAGATGGTCGTGTAC
TTGTACATGGAACGAAAGATATAAGTGAAGCAAAAACAGTTTATCATCGTTATTTTGGATAG

Upstream 100 bases:

>100_bases
CCTTTAGAAGGAATGAGCGTAGTTGAATAATCGATATTCTCGCCAAGAACTATTTTCTCCAATTGGAGAAGAAGGACAGC
AAAAGATAAGGGAAAAGCAT

Downstream 100 bases:

>100_bases
AAAAGGATGAGTGGGATGAAAGTAAATAAAGCTTTAACAATTGCAGGATCTGATAGTGGCGGCGGTGCCGGCATTCAAGC
AGATTTAAAAACATTCCAAG

Product: thiamine/molybdopterin biosynthesis thif/moeb-like protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYAESDVENNLPKAVAAKKRLEEINSEVRVEALVQDVT
AEELEELVTNVDVMIDATDNFETRFIVNDMAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTAGI
ISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNCPSCGENALYPYLNKENTSKTAVLCGR
NTVQIRPPHKEEIDFERYKELLHDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVLVHGTKDISEAKTVYHRYFG

Sequences:

>Translated_313_residues
MLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYAESDVENNLPKAVAAKKRLEEINSEVRVEALVQDVT
AEELEELVTNVDVMIDATDNFETRFIVNDMAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTAGI
ISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNCPSCGENALYPYLNKENTSKTAVLCGR
NTVQIRPPHKEEIDFERYKELLHDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVLVHGTKDISEAKTVYHRYFG
>Mature_313_residues
MLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYAESDVENNLPKAVAAKKRLEEINSEVRVEALVQDVT
AEELEELVTNVDVMIDATDNFETRFIVNDMAQKYSIPWIYGACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTAGI
ISPAVSLVVSHQVTEALKLLVEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNCPSCGENALYPYLNKENTSKTAVLCGR
NTVQIRPPHKEEIDFERYKELLHDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVLVHGTKDISEAKTVYHRYFG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=265, Percent_Identity=27.5471698113208, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI150417996, Length=148, Percent_Identity=30.4054054054054, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87082356, Length=226, Percent_Identity=34.070796460177, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787048, Length=223, Percent_Identity=30.9417040358744, Blast_Score=105, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17540406, Length=223, Percent_Identity=31.390134529148, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321903, Length=272, Percent_Identity=27.5735294117647, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=30, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24582879, Length=224, Percent_Identity=26.3392857142857, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 35009; Mature: 35009

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYAESDVENNLPKAVAAK
CEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCHHCCCHHHHHHH
KRLEEINSEVRVEALVQDVTAEELEELVTNVDVMIDATDNFETRFIVNDMAQKYSIPWIY
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCCEEHHHHHHHHHCCCCCHH
GACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLL
HHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNCPSCGENALYPYLNKENTSKTAVLCGR
HHHHHHHHCCCEEEEHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECC
NTVQIRPPHKEEIDFERYKELLHDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVLVHGTKD
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCEEEEECCCC
ISEAKTVYHRYFG
HHHHHHHHHHHCC
>Mature Secondary Structure
MLIIGAGALGSANAEMFVRAGVGTVTIVDRDYVDWSNLQRQQLYAESDVENNLPKAVAAK
CEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCHHCCCHHHHHHH
KRLEEINSEVRVEALVQDVTAEELEELVTNVDVMIDATDNFETRFIVNDMAQKYSIPWIY
HHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCCEEHHHHHHHHHCCCCCHH
GACVGSYGLSYTILPSKTPCLSCLLQSIPLGGATCDTAGIISPAVSLVVSHQVTEALKLL
HHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VEDYESLRDGLVSFDVWKNEYSCMNVQKLRKHNCPSCGENALYPYLNKENTSKTAVLCGR
HHHHHHHHCCCEEEEHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECC
NTVQIRPPHKEEIDFERYKELLHDRVNDLNVNPYLLSFSVEEKRLVAFKDGRVLVHGTKD
CEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCEEEEECCCC
ISEAKTVYHRYFG
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]