Click here to switch to the map view.

The map label for this gene is tenA [H]

Identifier: 222094478

GI number: 222094478

Start: 805685

End: 806380

Strand: Direct

Name: tenA [H]

Synonym: BCQ_0816

Alternate gene names: 222094478

Gene position: 805685-806380 (Clockwise)

Preceding gene: 222094476

Following gene: 222094479

Centisome position: 15.45

GC content: 35.34

Gene sequence:

>696_bases
ATGAAATTTTGCGATAGATTATTAGAAACTGTACAACCAGTTTGGGAGATGAGTCATAATCATCCATTTGTTGTAGGTAT
GGGGGATGGTACGTTAGAAAAAGATAAGTTTCAGTATTATATTATTCAAGATTATTTATACTTGTTAGATTATGCAAAGT
TATATGCGATCGGTGTTGTAAAGGCAACAAATCCACAAGTTATGGGGAAATTTGCTGAACAAATTGATGGGATATTAAAC
GGAGAAATGACAATTCATAAACAGTATGCAAAAAGACTTGGAATTTCTATAGAGGAGATAGAGTCTGCAAAGCCATCTGC
TAAAAATTTAGCTTATACAAATTACATGATGTCTGTATCTCAAAATGGCACACTTGCGGAATTAATAGCAGCACTTCTTC
CATGTATGTGGAGCTACTGGGAGATTGGAAAGCGTTTAAATGATATTCCTGGAGCAAGAGATCATGAGTTCTTTGGTGAG
TGGATTCAAGGCTATAGCTCTGAAGAATATGGCAACCTTTGTGTTTGGTTAATCGATTTATTAAATGAAATGGCAGTTGG
AAAGTCTGAAAAAGAGCTAGATCGATTAGAGGAGATTTTCCTATATTCCAGTCGATTTGAATATTTATTCTGGGATATGT
CCTACCGTAAGGAGATGTGGGGATTTGAGGAGCAAGAACATACTACAGTTTCATAA

Upstream 100 bases:

>100_bases
AAGTGGAGCAACGAATAAATTATAATTTTATTTGCTGTAACCACTTCTTATTTAGAAGTGGTTTTTTATTTAGATATATA
AATTGAAAGGGGATAGAGAA

Downstream 100 bases:

>100_bases
TGTTTCTTTTCATTATGATGAGAAGCCAATCATTAATGAATTAAATGCTTCTATACAAGATAAAGAGTTTGTAAGCATTA
TCGGACCGAGTGGATGCGGG

Product: transcriptional regulator of extracellular enzymes

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCVWLIDLLNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS

Sequences:

>Translated_231_residues
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCVWLIDLLNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
>Mature_231_residues
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVVKATNPQVMGKFAEQIDGILN
GEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVSQNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGE
WIQGYSSEEYGNLCVWLIDLLNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=206, Percent_Identity=32.0388349514563, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=205, Percent_Identity=30.2439024390244, Blast_Score=90, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325378, Length=207, Percent_Identity=28.5024154589372, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26950; Mature: 26950

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVV
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
KATNPQVMGKFAEQIDGILNGEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGEWIQGYSSEEYGNLCVWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
HHHHHCCCCHHHHHHHHHHHHHHCCCHHEEECHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKFCDRLLETVQPVWEMSHNHPFVVGMGDGTLEKDKFQYYIIQDYLYLLDYAKLYAIGVV
CCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEE
KATNPQVMGKFAEQIDGILNGEMTIHKQYAKRLGISIEEIESAKPSAKNLAYTNYMMSVS
ECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCCC
QNGTLAELIAALLPCMWSYWEIGKRLNDIPGARDHEFFGEWIQGYSSEEYGNLCVWLIDL
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
LNEMAVGKSEKELDRLEEIFLYSSRFEYLFWDMSYRKEMWGFEEQEHTTVS
HHHHHCCCCHHHHHHHHHHHHHHCCCHHEEECHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]