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The map label for this gene is amaA [H]

Identifier: 222094461

GI number: 222094461

Start: 778193

End: 779368

Strand: Direct

Name: amaA [H]

Synonym: BCQ_0775

Alternate gene names: 222094461

Gene position: 778193-779368 (Clockwise)

Preceding gene: 222094460

Following gene: 222094462

Centisome position: 14.92

GC content: 39.29

Gene sequence:

>1176_bases
ATGGATGTTGCAAAAGAACTTGTTTTGTCAAAGGATCAGTTGGTTGAGTGGAGAAGACATTTTCATAAGTATCCAGAGTT
ATCTTTTCAAGAAGAGAAAACATCGCAGTTTGTGTTCGATATACTTCGGGAAATTCCATGTTTAGAAGTATCAAGACCTA
CGAAATATAGTGTAATGGCAAGGTTGATTGGAAAGCAGCCTGGTAAAATAGTTGCGGTTCGAGCTGACATGGATGCTCTT
CCTATTCATGAAGAAAATGAGTTTGATTTTATTTCTACATATCCGGGTGTAATGCATGCATGTGGCCATGATGGACATAT
AGCGATATTACTTGGAGTTGTACATAAGTTAGTAGAAGCAAGAGAGAAAATTAAAGGGGAGATTCGTTTTCTATTCCAAC
ATGCTGAAGAAAACTTTCCTGGTGGTGCAGAGGAAATGGTTGCAGCGGGTGTAATGGAAGGGGTCGATTATATTATTGGT
GCTCACCTTTGGGCATCGTTAGAGGTTGGAAAAGTAGGTGTAATTTATGGTCCCGCAATGGCTGCTCCAGATGTTTTTAA
AATTATAATAGAAGGAAAAGGTGGACATGCTGGAATCCCTCATGAAACTGTTGATAGTATTGCAATCGGTACACAAGTGA
TTTCCCAACTTCAGCAAATTGTATCTCGCCTCACGAATCCGTTAGATTCTCTTGTAGTATCTGTTACGCAATTTCACGCT
GGGACAACCCATAATGTGATTCCAGAACAAGCGGAGATTGAAGGGACAGTGCGGAGTTTAAGACATGAGTTACGAGAAGA
AACAGAGAAAAGGATTGAACAGATTGTAAAGCATGTGACGGAAGCTTATGGAGCGAAATATACGTTTTCTTATGAATATG
GATATCGTCCAGTAGTAAATGATTATGAAGTGACAGAGATTATTGAGCAAACAGCATTACAGCTTTATGGAAGGGAACGA
GTTACTCGTTTACAGCCGACGATGGCTGGAGAAGATTTTTCAGCGTTTTTACAAAAAGCACCAGGGACATTCTTTTTTAT
CGGAGCAGGAAACAAAGAGAAAGGAATTATATATCCTCACCATCACCCTCGTTTTACGATTGATGAAGATGCATTACCAA
TTGGCGTGCAAGTCTTTGTATCATCGATTATGAATTTCATAAGTAAAGGAGAATGA

Upstream 100 bases:

>100_bases
ATTTTTAATTAATTATAACGGAAAATTCAGAAGATTCTTTTAAAAGTTAACGATTCACGTTATAATAACATTTAACTTGG
TGAAGAAGGGGAGAAGAGAA

Downstream 100 bases:

>100_bases
GATGAAGAAAATACACGTATTAGCGCTTATTCCAGTTTTTTGTTTAGTTGTTGGCCCCGTATTTGCTAATTCAGTTACTC
CTTACATATTAGGGATGCCA

Product: aminoacylase (n-acyl-l-amino acid amidohydrolase)

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILREIPCLEVSRPTKYSVMARLIGKQPGKIVAVRADMDAL
PIHEENEFDFISTYPGVMHACGHDGHIAILLGVVHKLVEAREKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIG
AHLWASLEVGKVGVIYGPAMAAPDVFKIIIEGKGGHAGIPHETVDSIAIGTQVISQLQQIVSRLTNPLDSLVVSVTQFHA
GTTHNVIPEQAEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEIIEQTALQLYGRER
VTRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPHHHPRFTIDEDALPIGVQVFVSSIMNFISKGE

Sequences:

>Translated_391_residues
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILREIPCLEVSRPTKYSVMARLIGKQPGKIVAVRADMDAL
PIHEENEFDFISTYPGVMHACGHDGHIAILLGVVHKLVEAREKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIG
AHLWASLEVGKVGVIYGPAMAAPDVFKIIIEGKGGHAGIPHETVDSIAIGTQVISQLQQIVSRLTNPLDSLVVSVTQFHA
GTTHNVIPEQAEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEIIEQTALQLYGRER
VTRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPHHHPRFTIDEDALPIGVQVFVSSIMNFISKGE
>Mature_391_residues
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILREIPCLEVSRPTKYSVMARLIGKQPGKIVAVRADMDAL
PIHEENEFDFISTYPGVMHACGHDGHIAILLGVVHKLVEAREKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIG
AHLWASLEVGKVGVIYGPAMAAPDVFKIIIEGKGGHAGIPHETVDSIAIGTQVISQLQQIVSRLTNPLDSLVVSVTQFHA
GTTHNVIPEQAEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVNDYEVTEIIEQTALQLYGRER
VTRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPHHHPRFTIDEDALPIGVQVFVSSIMNFISKGE

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=424, Percent_Identity=25.4716981132075, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43794; Mature: 43794

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILREIPCLEVSRPTKYSVMA
CCHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
RLIGKQPGKIVAVRADMDALPIHEENEFDFISTYPGVMHACGHDGHIAILLGVVHKLVEA
HHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHH
REKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIGAHLWASLEVGKVGVIYGPAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
AAPDVFKIIIEGKGGHAGIPHETVDSIAIGTQVISQLQQIVSRLTNPLDSLVVSVTQFHA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GTTHNVIPEQAEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DYEVTEIIEQTALQLYGRERVTRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEECCC
HHPRFTIDEDALPIGVQVFVSSIMNFISKGE
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVAKELVLSKDQLVEWRRHFHKYPELSFQEEKTSQFVFDILREIPCLEVSRPTKYSVMA
CCHHHHHHHCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
RLIGKQPGKIVAVRADMDALPIHEENEFDFISTYPGVMHACGHDGHIAILLGVVHKLVEA
HHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHH
REKIKGEIRFLFQHAEENFPGGAEEMVAAGVMEGVDYIIGAHLWASLEVGKVGVIYGPAM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
AAPDVFKIIIEGKGGHAGIPHETVDSIAIGTQVISQLQQIVSRLTNPLDSLVVSVTQFHA
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCC
GTTHNVIPEQAEIEGTVRSLRHELREETEKRIEQIVKHVTEAYGAKYTFSYEYGYRPVVN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCC
DYEVTEIIEQTALQLYGRERVTRLQPTMAGEDFSAFLQKAPGTFFFIGAGNKEKGIIYPH
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCEECCC
HHPRFTIDEDALPIGVQVFVSSIMNFISKGE
CCCCEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]