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The map label for this gene is brnQ [H]

Identifier: 222094445

GI number: 222094445

Start: 759960

End: 761144

Strand: Direct

Name: brnQ [H]

Synonym: BCQ_0759

Alternate gene names: 222094445

Gene position: 759960-761144 (Clockwise)

Preceding gene: 222094444

Following gene: 222094446

Centisome position: 14.57

GC content: 36.12

Gene sequence:

>1185_bases
GTGTGGATTTCATTAGTAGGGTTTATTATATCAGCAACAGGTCTTCCGATATTGGGAGTTATTGCGATTGCAAAAGCAGG
AAGTTTTCAAACGCTAGCGGGTAGAGTTCATTCTTCATTCGCCATTATCTTTCCGTGTATCGTGTATGTATTTATTGGAC
CAGGCCTTGGTATTCCGCGTGCAGGAAGTCTAGCTTTTGAAATGGGACCGGGTCAGCTATTTCCGGAAGCGGGTAGCGTA
GTCTTATTATGTTACACAGTTATTTTCTTTAGTATTGTGTACTGGTTAAGTTTATCACCGTCTAAATTAATGGGGTTGTT
CGGAAAAGTTTTAACACCGTTATTATTAGGTATGATTGCTCTTATCTTTATAAAAAGCATGTTTACATCAGTAGGTAGTG
TGAAAGAGGCTGCTGGAAATTATGGACAAGCGCCTATGTTCCAAGGGTTTTTAGATGGATATTTAACGATGGATGCATTA
GCAGCTTTAATTTTTGGGATTGTGATTGCAAATGCTCTTCGTGCAAAAGGTATTGAAGATGATAAAGGTTTAGCGAAATA
TATGAGTATTGCTGGAATTGGTGCAGGACTATTATTATCTATTATTTATGTCATACTTGGATATGTCGGTTCAATGAGTG
GTTCATTGGGGACATTTGATAATGGCGCGCAAGTGTTAGCGCAAGTGATGACTACATTATTTGGGCAAGGCGGGGTCGTT
TTATTAGGTCTTATTTTTACGATCGCGTGTTTATGTGTGTCAATCGGACTTGTTACATCTTGTAGTCAATTTTTCGCAAG
TGCATTTCCGAAAGTAGCTTATAAAGTTTGGGCATTTATATTAAGTTTGGTTAGTATGATTTTAGCTAATTTAGGATTAA
CACAAATTTTAAAAGTATCAGTACCGATTCTTGGATTTATTTATCCAGTTGCATTAACGCTTATTATTTTAGGGCTATTC
CATAAGTATATTGAGAAGTATGCATACGTATATACAGTAACTGTTGGAATTGTAGCGGTGTTTAGTGCAGTTGATATTTT
CAATAAAAATGTGATGATGAATCAGTGGACATCAGTATTGAAATACATTCCGTTTTATATGGAAGGTGTTGGGTGGATTG
TTCCAGCTATTATAGGTGCTTGTATTGGTGTTATCGTGAGTATTGTTATAAATAAGAATAAATAA

Upstream 100 bases:

>100_bases
AAAAGAAATTTTACTTATTAGTCTTATGTTATTCTCGATGTTCTTTGGAGCGGGGAATCTTATATTTCCACCTTTTCTTG
GGTATGAAGCAGGAGAACAT

Downstream 100 bases:

>100_bases
GTAAAAGGTGTTTTCCGATTTCGGAAAACACCTTTCTTTTTTGGAAAAATAGTATTGAAAATGAATAGACTAAGAGTATG
ATAAAGGTAATTGGTAATAT

Product: branched-chain amino acid transporter ii carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MWISLVGFIISATGLPILGVIAIAKAGSFQTLAGRVHSSFAIIFPCIVYVFIGPGLGIPRAGSLAFEMGPGQLFPEAGSV
VLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLGMIALIFIKSMFTSVGSVKEAAGNYGQAPMFQGFLDGYLTMDAL
AALIFGIVIANALRAKGIEDDKGLAKYMSIAGIGAGLLLSIIYVILGYVGSMSGSLGTFDNGAQVLAQVMTTLFGQGGVV
LLGLIFTIACLCVSIGLVTSCSQFFASAFPKVAYKVWAFILSLVSMILANLGLTQILKVSVPILGFIYPVALTLIILGLF
HKYIEKYAYVYTVTVGIVAVFSAVDIFNKNVMMNQWTSVLKYIPFYMEGVGWIVPAIIGACIGVIVSIVINKNK

Sequences:

>Translated_394_residues
MWISLVGFIISATGLPILGVIAIAKAGSFQTLAGRVHSSFAIIFPCIVYVFIGPGLGIPRAGSLAFEMGPGQLFPEAGSV
VLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLGMIALIFIKSMFTSVGSVKEAAGNYGQAPMFQGFLDGYLTMDAL
AALIFGIVIANALRAKGIEDDKGLAKYMSIAGIGAGLLLSIIYVILGYVGSMSGSLGTFDNGAQVLAQVMTTLFGQGGVV
LLGLIFTIACLCVSIGLVTSCSQFFASAFPKVAYKVWAFILSLVSMILANLGLTQILKVSVPILGFIYPVALTLIILGLF
HKYIEKYAYVYTVTVGIVAVFSAVDIFNKNVMMNQWTSVLKYIPFYMEGVGWIVPAIIGACIGVIVSIVINKNK
>Mature_394_residues
MWISLVGFIISATGLPILGVIAIAKAGSFQTLAGRVHSSFAIIFPCIVYVFIGPGLGIPRAGSLAFEMGPGQLFPEAGSV
VLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLGMIALIFIKSMFTSVGSVKEAAGNYGQAPMFQGFLDGYLTMDAL
AALIFGIVIANALRAKGIEDDKGLAKYMSIAGIGAGLLLSIIYVILGYVGSMSGSLGTFDNGAQVLAQVMTTLFGQGGVV
LLGLIFTIACLCVSIGLVTSCSQFFASAFPKVAYKVWAFILSLVSMILANLGLTQILKVSVPILGFIYPVALTLIILGLF
HKYIEKYAYVYTVTVGIVAVFSAVDIFNKNVMMNQWTSVLKYIPFYMEGVGWIVPAIIGACIGVIVSIVINKNK

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=390, Percent_Identity=33.3333333333333, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 41859; Mature: 41859

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWISLVGFIISATGLPILGVIAIAKAGSFQTLAGRVHSSFAIIFPCIVYVFIGPGLGIPR
CHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AGSLAFEMGPGQLFPEAGSVVLLCYTVIFFSIVYWLSLSPSKLMGLFGKVLTPLLLGMIA
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIFIKSMFTSVGSVKEAAGNYGQAPMFQGFLDGYLTMDALAALIFGIVIANALRAKGIED
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DKGLAKYMSIAGIGAGLLLSIIYVILGYVGSMSGSLGTFDNGAQVLAQVMTTLFGQGGVV
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
LLGLIFTIACLCVSIGLVTSCSQFFASAFPKVAYKVWAFILSLVSMILANLGLTQILKVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VPILGFIYPVALTLIILGLFHKYIEKYAYVYTVTVGIVAVFSAVDIFNKNVMMNQWTSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KYIPFYMEGVGWIVPAIIGACIGVIVSIVINKNK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]