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The map label for this gene is celF [H]

Identifier: 22127820

GI number: 22127820

Start: 4407371

End: 4408684

Strand: Reverse

Name: celF [H]

Synonym: y3950

Alternate gene names: 22127820

Gene position: 4408684-4407371 (Counterclockwise)

Preceding gene: 22127825

Following gene: 22127819

Centisome position: 95.83

GC content: 50.46

Gene sequence:

>1314_bases
ATGAGCACGTTTAAAATAACCATTATTGGCGGTGGGAGTAGCTATACCCCAGAGCTAGTTGATGGGTTGATTCAGCGTAT
TGAACAACTGCCCGTCACTGAACTGGCACTGGTTGATGTCGAGCAGGGCCGCCAAAAGGTGGAAATTATTGCCGCGCTGA
CCCAAAGAATGCTTGCCCGTCATGGGTTGGAACAGGTCAAAGTCAGTGTCCACTACTCGCTGGATGAAGCCATTCGCGGT
GCCCGTTTTGTCCTGACACAATTTCGTGTCGGCCAACTCCCTGCCAGGGCAGCAGACGAACGCTTGGGGTTAAAATATAA
CCTGCTCGGTCAGGAGACGACCGGAATTGGTGGCTTTGCCAAAGCGCTGCGTACCATTCCGGTGATGCTTGATATTGCTG
CTAACGTTGAGCGATTGGCCCCCGATGCCTGGATCATTAACTTCACTAACCCAGCCGGCATTGTCACCGAAGCCGTTAGC
CGCTATACCAAAGCGAAAATCATTGGTCTGTGTAATGTCCCGATCAGTATGCATCATATGATTGCCAAGCTCTTGCAAGC
CCCCTACGAAGACCTCCAACTGCGCTTTGCCGGGCTGAATCATATGGTATGGGTACATGAAGTATTACAACGCGGGAAAT
ATGTCACCGCTGATGTGCTGAAAATGCTGTGTGATGGGGCATCATTAAGCATGAACAATATTAAGGAAGCCCCATGGCCA
CCCGAGTTCCTGCAAGCAATGGGGGCCATCCCCTGCCCATACCATCGCTATTTCTACCAAACGCAAGACATGCTGGCAGA
AGAGATGGCCGCAGCAGCTGAGCGTGGTACCCGTGCGGAACAGGTGATGCAGGTGGAAAAAGAGCTGTTCGCGCTGTACG
CCGATCCGCATTTGGATACCAAACCAGAGCAGCTCAGCTTCCGGGGTGGATCATTTTATTCTGAAGTTGCATTGGAACTT
ATTCGTGCCATCCATAACAACTTAGGGACGCAATTAGTAGTAAATACAACAAACCGTGGCGCGATTCGCGGTTTATCTGA
TGATTCTGTGGTAGAAACTAACTGTATTATTGATGCAAAAGGGGCTCATCCATTAACCTTCGGCCCTTTACCGGTCTCTA
TGCATGGGCTAACCCAACAGGTAAAAGCCTATGAACGATTGACGATTGACGCGGCGGTACATGGCGATCGTCGCAGTGCC
TTGTTGGCCTTAGTGACAAATCCACTGATTGCTAACGCCAGCATTGCTCAACCGTTACTGGAGGAGGTACTACAGGTGAA
TAAACCTTACCTGCCTCAGTTCTCCGGTTTGTGA

Upstream 100 bases:

>100_bases
TGGTGAGCCTGAATTTATCCACAGATATGTTATTCACAAACATGTTACCCACAGACATGACTGAACTTTATCGCCGGCAG
AGCCGTTTGGAGCCCCTACA

Downstream 100 bases:

>100_bases
TGACCTAGCCAACGTCATTGGTGTTGTAGCACTATCAGTTCGGTTTATAACGACATCAGCTTGTTTGTAGCGACATTGGT
TTTTTTGTAACAATATCAGT

Product: phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSTFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLARHGLEQVKVSVHYSLDEAIRG
ARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFAKALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVS
RYTKAKIIGLCNVPISMHHMIAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKYVTADVLKMLCDGASLSMNNIKEAPWP
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDTKPEQLSFRGGSFYSEVALEL
IRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAKGAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSA
LLALVTNPLIANASIAQPLLEEVLQVNKPYLPQFSGL

Sequences:

>Translated_437_residues
MSTFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLARHGLEQVKVSVHYSLDEAIRG
ARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFAKALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVS
RYTKAKIIGLCNVPISMHHMIAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKYVTADVLKMLCDGASLSMNNIKEAPWP
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDTKPEQLSFRGGSFYSEVALEL
IRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAKGAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSA
LLALVTNPLIANASIAQPLLEEVLQVNKPYLPQFSGL
>Mature_436_residues
STFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLARHGLEQVKVSVHYSLDEAIRGA
RFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFAKALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVSR
YTKAKIIGLCNVPISMHHMIAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKYVTADVLKMLCDGASLSMNNIKEAPWPP
EFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDTKPEQLSFRGGSFYSEVALELI
RAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAKGAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSAL
LALVTNPLIANASIAQPLLEEVLQVNKPYLPQFSGL

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=440, Percent_Identity=43.1818181818182, Blast_Score=345, Evalue=3e-96,
Organism=Escherichia coli, GI1790560, Length=440, Percent_Identity=26.3636363636364, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 48154; Mature: 48023

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLAR
CCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHHHHHHH
HGLEQVKVSVHYSLDEAIRGARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFA
CCHHHEEEEEEECHHHHHCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCCHHHH
KALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVSRYTKAKIIGLCNVPISMHHM
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHH
IAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKYVTADVLKMLCDGASLSMNNIKEAPWP
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCCCCCC
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
KPEQLSFRGGSFYSEVALELIRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAK
CCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCEEEEEEEEECC
GAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSALLALVTNPLIANASIAQPLL
CCCCCEECCCCCCHHHHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHCCHHCCCHHHHHHH
EEVLQVNKPYLPQFSGL
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLAR
CEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHHHHHHH
HGLEQVKVSVHYSLDEAIRGARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFA
CCHHHEEEEEEECHHHHHCHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCCHHHH
KALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVSRYTKAKIIGLCNVPISMHHM
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHH
IAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKYVTADVLKMLCDGASLSMNNIKEAPWP
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCCCCCCC
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
KPEQLSFRGGSFYSEVALELIRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAK
CCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCEEEEEEEEECC
GAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSALLALVTNPLIANASIAQPLL
CCCCCEECCCCCCHHHHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHCCHHCCCHHHHHHH
EEVLQVNKPYLPQFSGL
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]