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The map label for this gene is mcrB [H]

Identifier: 22127668

GI number: 22127668

Start: 4215478

End: 4217544

Strand: Reverse

Name: mcrB [H]

Synonym: y3797

Alternate gene names: 22127668

Gene position: 4217544-4215478 (Counterclockwise)

Preceding gene: 22127671

Following gene: 22127667

Centisome position: 91.67

GC content: 47.61

Gene sequence:

>2067_bases
GTGATGGTTCAGGATTCCTCTGCGTTAGCCTCTTTCTTTCGTTCTCATATCGCAAAAAATAATCAATACAGCCCCGACTG
GGGCAAACACTATCGTCAGCTCGTAGAACAGGTCCAAGCCGAAAAACCCAACTTCTCTGCGGATACAATTCGGCAGATTT
GGTACGAACGTAGCAATGGTATCTCCAGCCTCAGGCAGGGCGGTATGTCCCAGTTTGAATTCGAAACGGCGCAAAAACAG
CTGCTCGCTCTCACCCGTCAGATGGCAGATGAGTGTACGCAGGAGAACTACGATCGGGTGATTGAGCGCCTTGTTAAACT
TAAAACAGATGGGATATTAAACAAAGTATATTGGGCACTTTGCCATCGTGCCTTTGCCGCGCTCTACCCGTCGAAAATTA
CCAACGTTGTGAATGTCAGCAATTTTTTCAGCAGTTATAACTATTGCAATAACCATTTTCAACTTGACCTTTCTGAGGAT
CAGGAGTGGTTCGCTCGCAACCTTGATCTCAAGAAAGTGTTGCATCGAGCATTGGGCGATGATGTCGATCCTATCGAACT
GAATATGTCGCTCTGGCATCTCTACACGCAGGTGATTCAGAAGAAGAATGATATTGGGTTAATCGGTAGTGAGACTGCCT
TGCCAGAGGATGAGCAAGACGAGGAGGTTACCACTCCGCAACTGCCAAAAAATACTATCCTCTACGGACCTCCAGGTACC
GGTAAAACTTACTGTACCATCGAGCTAGCCGTACGCGCCTGCGAACCAGCAGCCTACTCGCTGCAGGAAGGCAAAGAAGA
GAACGAAAAACGCCGTGAGCTGAAAAAGGTTTATGACCGACTGATTGCTGAAAAGCGGGTGCGCTTTATCACCTTCCATC
AGAGTTTTGGCTATGAAGAGTTTATCGAAGGGCTAAGAGCAGAAACCACCGATGATGGCAATGTACGCTATGAAGTCAAA
GCGGGCATTTTTAAGCAAATTTGTGAAGATGCTGCTTTTGGTCACGCCGGTGTTCAGCAGAAGCTTGACGAGGCGCTTGC
CCGCCTACAAGAACGTTTATCTGAAAGTGGAAGCATTACCCTTGAAACGCTGCAGGGCAAAGCCTTCCAACTGGCTTACA
AGAGCCAGACCACCTTTGGTATTTTCCCGTCGCAATCGAAAAAAGAGGATTTAGGGCAGGGTTACAATGCTTACCTCAAG
AATATCAGTCTGGTTTACCAAAACCCTCAGGCAAAGGTACACAATCCTTCATATGTACGTAGTATCCTTAATTACCTCAT
TAAACAGGAAGGTTTGCCTTCCAACCCACAGGAATCTGTATCTGAAAAACGGCAAAACTACGTTCTGATTATTGACGAGA
TTAACCGTGGCAACATCTCCAAAATCTTCGGCGAACTGATCACCCTGATAGAAACCTCTAAACGTGCCGGGGAACCGGAG
GCGCTTAGCGTGATTCTGCCTTACTCTTCCAGTTCGTTCAGCGTACCGAATAATCTCTATTTAATCGGCACCATGAACAC
TGCTGACCGTTCGCTCACCGCACTGGATACCGCCCTGCGTCGCCGATTCGAGTTTGAAGCGATGCTGCCGGATATCACCG
TTCTAAAAGAAACTGTTGTCAAAGGTATTGATCTGCCACGTCTACTGCAGACCTTGAATGACCGCATTGAGGTGCTGTAC
GACAGAGAACATACACTAGGCCATGCCTTTTTCATCCCAGTAGTTCAGGTAAAAGAGGACGAAGACCTGGCATTTGAAAG
GCTTAAGCGGATCATGCGAAACAAGGTGCTGCCTCTGTTGGAGGAGTACTTCTACAACGACTGGCAAAAAATTCGTATGG
TGTTGGGCGACAATCAGAAAAGCGGAAACCCGCAGCTACAGTTCGTGTGCGAGGTGAAAGACCAGAAACAGTTTGCCGAT
CTTTTTGGCAATAACGGAACTGAAGATCTGCATGATATAGGTGCCAGTTTCCATCTAGCCCCTGAGGACGACAAGGTTTG
GGATAACCCGCTCGCCTGGCAACAAATTTATGCGCCACATAAAGGTAACCCGGTGAGTGGGGAATGA

Upstream 100 bases:

>100_bases
CCCCTGGATCTGATTTCAGGCGTTGGGTGTGGATCACTATTGCACCGTTCGTTACAAGATACAGATATCTCATTTTTTAA
TCGCCCTACACACGGAGTAT

Downstream 100 bases:

>100_bases
AGTCTCGTAAGGACTTTACCGTCTTCGAATATGGTCACCTGATTAGCGACAAAGACAGTGGCCGCATAAATAATGCGGTC
TCGCTGCCTCCGCAGCTGTT

Product: hypothetical protein

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MMVQDSSALASFFRSHIAKNNQYSPDWGKHYRQLVEQVQAEKPNFSADTIRQIWYERSNGISSLRQGGMSQFEFETAQKQ
LLALTRQMADECTQENYDRVIERLVKLKTDGILNKVYWALCHRAFAALYPSKITNVVNVSNFFSSYNYCNNHFQLDLSED
QEWFARNLDLKKVLHRALGDDVDPIELNMSLWHLYTQVIQKKNDIGLIGSETALPEDEQDEEVTTPQLPKNTILYGPPGT
GKTYCTIELAVRACEPAAYSLQEGKEENEKRRELKKVYDRLIAEKRVRFITFHQSFGYEEFIEGLRAETTDDGNVRYEVK
AGIFKQICEDAAFGHAGVQQKLDEALARLQERLSESGSITLETLQGKAFQLAYKSQTTFGIFPSQSKKEDLGQGYNAYLK
NISLVYQNPQAKVHNPSYVRSILNYLIKQEGLPSNPQESVSEKRQNYVLIIDEINRGNISKIFGELITLIETSKRAGEPE
ALSVILPYSSSSFSVPNNLYLIGTMNTADRSLTALDTALRRRFEFEAMLPDITVLKETVVKGIDLPRLLQTLNDRIEVLY
DREHTLGHAFFIPVVQVKEDEDLAFERLKRIMRNKVLPLLEEYFYNDWQKIRMVLGDNQKSGNPQLQFVCEVKDQKQFAD
LFGNNGTEDLHDIGASFHLAPEDDKVWDNPLAWQQIYAPHKGNPVSGE

Sequences:

>Translated_688_residues
MMVQDSSALASFFRSHIAKNNQYSPDWGKHYRQLVEQVQAEKPNFSADTIRQIWYERSNGISSLRQGGMSQFEFETAQKQ
LLALTRQMADECTQENYDRVIERLVKLKTDGILNKVYWALCHRAFAALYPSKITNVVNVSNFFSSYNYCNNHFQLDLSED
QEWFARNLDLKKVLHRALGDDVDPIELNMSLWHLYTQVIQKKNDIGLIGSETALPEDEQDEEVTTPQLPKNTILYGPPGT
GKTYCTIELAVRACEPAAYSLQEGKEENEKRRELKKVYDRLIAEKRVRFITFHQSFGYEEFIEGLRAETTDDGNVRYEVK
AGIFKQICEDAAFGHAGVQQKLDEALARLQERLSESGSITLETLQGKAFQLAYKSQTTFGIFPSQSKKEDLGQGYNAYLK
NISLVYQNPQAKVHNPSYVRSILNYLIKQEGLPSNPQESVSEKRQNYVLIIDEINRGNISKIFGELITLIETSKRAGEPE
ALSVILPYSSSSFSVPNNLYLIGTMNTADRSLTALDTALRRRFEFEAMLPDITVLKETVVKGIDLPRLLQTLNDRIEVLY
DREHTLGHAFFIPVVQVKEDEDLAFERLKRIMRNKVLPLLEEYFYNDWQKIRMVLGDNQKSGNPQLQFVCEVKDQKQFAD
LFGNNGTEDLHDIGASFHLAPEDDKVWDNPLAWQQIYAPHKGNPVSGE
>Mature_688_residues
MMVQDSSALASFFRSHIAKNNQYSPDWGKHYRQLVEQVQAEKPNFSADTIRQIWYERSNGISSLRQGGMSQFEFETAQKQ
LLALTRQMADECTQENYDRVIERLVKLKTDGILNKVYWALCHRAFAALYPSKITNVVNVSNFFSSYNYCNNHFQLDLSED
QEWFARNLDLKKVLHRALGDDVDPIELNMSLWHLYTQVIQKKNDIGLIGSETALPEDEQDEEVTTPQLPKNTILYGPPGT
GKTYCTIELAVRACEPAAYSLQEGKEENEKRRELKKVYDRLIAEKRVRFITFHQSFGYEEFIEGLRAETTDDGNVRYEVK
AGIFKQICEDAAFGHAGVQQKLDEALARLQERLSESGSITLETLQGKAFQLAYKSQTTFGIFPSQSKKEDLGQGYNAYLK
NISLVYQNPQAKVHNPSYVRSILNYLIKQEGLPSNPQESVSEKRQNYVLIIDEINRGNISKIFGELITLIETSKRAGEPE
ALSVILPYSSSSFSVPNNLYLIGTMNTADRSLTALDTALRRRFEFEAMLPDITVLKETVVKGIDLPRLLQTLNDRIEVLY
DREHTLGHAFFIPVVQVKEDEDLAFERLKRIMRNKVLPLLEEYFYNDWQKIRMVLGDNQKSGNPQLQFVCEVKDQKQFAD
LFGNNGTEDLHDIGASFHLAPEDDKVWDNPLAWQQIYAPHKGNPVSGE

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=186, Percent_Identity=37.0967741935484, Blast_Score=109, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 78800; Mature: 78800

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVQDSSALASFFRSHIAKNNQYSPDWGKHYRQLVEQVQAEKPNFSADTIRQIWYERSNG
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
ISSLRQGGMSQFEFETAQKQLLALTRQMADECTQENYDRVIERLVKLKTDGILNKVYWAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CHRAFAALYPSKITNVVNVSNFFSSYNYCNNHFQLDLSEDQEWFARNLDLKKVLHRALGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCHHHHHHHHHCC
DVDPIELNMSLWHLYTQVIQKKNDIGLIGSETALPEDEQDEEVTTPQLPKNTILYGPPGT
CCCCEEEHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
GKTYCTIELAVRACEPAAYSLQEGKEENEKRRELKKVYDRLIAEKRVRFITFHQSFGYEE
CCEEEEEEEEHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCHHH
FIEGLRAETTDDGNVRYEVKAGIFKQICEDAAFGHAGVQQKLDEALARLQERLSESGSIT
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LETLQGKAFQLAYKSQTTFGIFPSQSKKEDLGQGYNAYLKNISLVYQNPQAKVHNPSYVR
EEEECCCEEEEEECCCCEEEECCCCCCHHHHCCCHHHHHHCCEEEEECCCCCCCCHHHHH
SILNYLIKQEGLPSNPQESVSEKRQNYVLIIDEINRGNISKIFGELITLIETSKRAGEPE
HHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
ALSVILPYSSSSFSVPNNLYLIGTMNTADRSLTALDTALRRRFEFEAMLPDITVLKETVV
EEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KGIDLPRLLQTLNDRIEVLYDREHTLGHAFFIPVVQVKEDEDLAFERLKRIMRNKVLPLL
HCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
EEYFYNDWQKIRMVLGDNQKSGNPQLQFVCEVKDQKQFADLFGNNGTEDLHDIGASFHLA
HHHHHCHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHCCCCEECC
PEDDKVWDNPLAWQQIYAPHKGNPVSGE
CCCCCCCCCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMVQDSSALASFFRSHIAKNNQYSPDWGKHYRQLVEQVQAEKPNFSADTIRQIWYERSNG
CCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
ISSLRQGGMSQFEFETAQKQLLALTRQMADECTQENYDRVIERLVKLKTDGILNKVYWAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CHRAFAALYPSKITNVVNVSNFFSSYNYCNNHFQLDLSEDQEWFARNLDLKKVLHRALGD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCHHHHHHHHHCC
DVDPIELNMSLWHLYTQVIQKKNDIGLIGSETALPEDEQDEEVTTPQLPKNTILYGPPGT
CCCCEEEHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
GKTYCTIELAVRACEPAAYSLQEGKEENEKRRELKKVYDRLIAEKRVRFITFHQSFGYEE
CCEEEEEEEEHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCHHH
FIEGLRAETTDDGNVRYEVKAGIFKQICEDAAFGHAGVQQKLDEALARLQERLSESGSIT
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEE
LETLQGKAFQLAYKSQTTFGIFPSQSKKEDLGQGYNAYLKNISLVYQNPQAKVHNPSYVR
EEEECCCEEEEEECCCCEEEECCCCCCHHHHCCCHHHHHHCCEEEEECCCCCCCCHHHHH
SILNYLIKQEGLPSNPQESVSEKRQNYVLIIDEINRGNISKIFGELITLIETSKRAGEPE
HHHHHHHHHCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
ALSVILPYSSSSFSVPNNLYLIGTMNTADRSLTALDTALRRRFEFEAMLPDITVLKETVV
EEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
KGIDLPRLLQTLNDRIEVLYDREHTLGHAFFIPVVQVKEDEDLAFERLKRIMRNKVLPLL
HCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
EEYFYNDWQKIRMVLGDNQKSGNPQLQFVCEVKDQKQFADLFGNNGTEDLHDIGASFHLA
HHHHHCHHHHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCHHHHCCCCEECC
PEDDKVWDNPLAWQQIYAPHKGNPVSGE
CCCCCCCCCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]