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The map label for this gene is ptsA [H]

Identifier: 22127647

GI number: 22127647

Start: 4196274

End: 4198910

Strand: Reverse

Name: ptsA [H]

Synonym: y3776

Alternate gene names: 22127647

Gene position: 4198910-4196274 (Counterclockwise)

Preceding gene: 22127649

Following gene: 22127639

Centisome position: 91.27

GC content: 52.6

Gene sequence:

>2637_bases
TTGACATTTTTATCTCATAGCCGCGAGGGGATAGCTGCTTTTATTCTGGTTGCCAGCCAGCGAGGGGAGGAAAGCCCAAT
GACCAAGCAAGTGACATTTATCTGCGGGCTTCTTAATGGCGTTCATGCCCGACCTGCGAGCCATATAGAAAGGGTATGCA
ACCGCTTCCAATCGCGTTTCCACTGGTATAATCCGCGTTCTGGCATCGTGGGAGATGGCAAGAGCGTACTTTCGCTGATC
GCGGCCAATATCCTGTTAGGGGATGAATGTCAGGTCACCATTGAAGGCGAAGATGAGCAGGTTGCTTTTGAACGCCTGAG
TATTTTTATTGAGCACGAGCTTCCACACGCTGACCCCATCTTGCCGAAGAGGGAGGAGAGTGCAGAATGGGAGCCGCTCC
CCGCCTCGTTAGCCCACTTGCACCCCACCCTGTTACGCGCCCGTTCAGTCAGTCCAGGTACCGCCTGCGGGAAGTTGCTG
TCTCTGATAAGAGCCGATCTCAATGCATTGGGTGATTTACCCGTGGCGCAAGGTATCGAGAGGGAGCAGCAGATGCTTGC
CGATGGGGTGGCGCAGCTAGGTAAAGCGTGGGAGAGCTTATTAGTCGCCAATAGCAGTACGGCAGCCAATAGCAGTACGA
CAGAAAATAACAGTACGACAGAAAATAACAGCACGACGCGAGCGATACGGGAAGTACACCGCTCGCTGTTGCGTGATGGC
ACATTTCGTCAACGGCTATTGAGTCACATAGTAGCAGGTGAAAGCTGTGCGACAGCCATCGTCGCTACTGCGGCCTATTT
CAGCCAGCAGTTGGCGCTTGCTGCTAACACTTATCTACGTGAACGTGAGTTGGATATTCGAGACGTCAGTTTTCAATTGT
TACAGCAGATTTATGGTGAGCAACGTTTTCCCTCCCAGCAAGCATTGAGCGAAGACAGTCTCTGCATTGCCGATGAGTTA
ACCCCGAGCCAATTCCTGGCGCTGGATAAACGTTATCTAAAGGGGTTGCTATTGGGCCGTGGTGGCAGTACTTCACATAC
GGTGATTCTGGCGCGTTCATTCAATATTCCGACGCTCGTGGGTGTCGATGCTGCGGCACTCCAGCCGTATATTAATCAAT
CGCTGCAGATTGATGGTGAGCTCGGGCTGGTGGTCTGTCTTCTGGATGAACCGGTACGCCGTTACTATCGGCAGGAGCAG
TGGCTACACGACCAACTGCGTGAGCAGCAGAGCCGATACCAGAATATGCCTGGGCGTACTCTTGATGGTGTCAGAATGGT
GGTGGCGGCTAACATCACCCATGCGGTAGAGGTGGAGGGGGCTTTCAATCAAGGTGCCGAATCCATTGGCTTGTTTCGTA
CTGAAATATTGTATATGGATCGTGCAGCCGCGCCTTCAGAAGAGGAGCTTTATACCCTCTACGCTCAGGCACTTGGCGCT
GCCAAGGGCAAACCGATTATTATTCGCACCATCGATATTGGCGGTGATAAACCGGTGAGTTATCTGAATATTCCTGCGGA
GAGCAACCCGTTTCTGGGCTACCGCGCGGTGCGTATTTATCACGAGTTTCTGTCGCTATTTCATACTCAATTGCGGGCGA
TCCTGCGGGCGTCAATGCATGGCCCGCTCAAAATCATGATCCCGATGATCTCCTCAATGGAGGAGATCCTGTGGGTGAAA
GATCAACTGGCAGAGGTGAAACAATCTCTGCGTATCAGTCATCTTCAGTTCGACGAAACTGTCCCCTTGGGTATGATGCT
GGAAGTGCCTTCAGTGATGTTCATCATCGATCAATGCTGTGAGGAAATGGATTTTTTGAGTATTGGCAGTAACGACCTGA
CACAGTACCTGCTGGCTGTTGATCGCGATAATGCCAAGGTGAGTGAGCACTATCACTGCTTGCCCCCGGCACTCCTACGC
GCTCTCGATTACGCGGTGTGCGAGGTACATCGCCACGGTAAGTGGATTGGCTTATGTGGTGAACTGGCGGCAAAAGACTC
GGTATTACCTCTGTTGGTAGCGATGGGGCTAGATGAGATCAGCATGAGTGCCTCTTTTATTGGCGCGACTAAAGCTCGCC
TTGCCAAACTTGATCGCGGCGAGTGCCGCCTGCTGCTCAACCGGGTGATGGCTTGCCGCACATCACGGGAAGTAGAATAT
CTGCTGGTGCAGTACGACGGGGAACAACGTGATGAACCACTGATTATTCCTGAATGTATTACTCTCGGCGCAGACTGGCG
TAGCAAAGAAGAGGTGATCAAAGGGATGGTGGATAACCTGCTGCTGGCTGGACGTTGCCGGTATCCGCGCAAACTGGCTG
CGGATGTGTGGGCGCGAGAAGCCCTTTTTTCCACTGGATTGGGCTTTGGGTTTGCCATTCCGCATGCTCAATCTGAGCAT
ATTGAGCAGTCGACGATCAGCGTGGCCAAGTTGGCAAAACCGGTGAATTGGGGAGAGGAAGACGCACTGTTTGTGATGAT
GTTAACCCTGAACAAACAGGCTTCGGGTGACCAGCATATGCGTATTTTTTCCAAACTGGCGCGTCGGATCATGCACGAGG
GGTTCCGCGATGCGCTGGTTAGCGCTGGGACGGCCCAGCAAGTAGAAATGCTGCTGAAACACGAGCTCGAACTGTGA

Upstream 100 bases:

>100_bases
AGCCATCTCAACTTCCCGGCAATGAGTGAGTTCAAATCATTACATGAACACTTTGTGAGCAATATCCCAATTATCGCTTG
TGATTACATTTGTCCAGTAT

Downstream 100 bases:

>100_bases
TTCTTTCATTTCAACATGACGGGTGCCGCGTCTGAGGTGGCACCCAGTGACCCTTATCGGATTACTTCAATAAGAATGAC
TTTCCTTGCTTAAGCACTAA

Product: PEP-protein phosphotransferase system enzyme I

Products: NA

Alternate protein names: MTP 2; Phosphoenolpyruvate-protein phosphotransferase; Enzyme I-Ani; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-like phosphotransferase enzyme IIA component; PTS system fructose-like EIIA component [H]

Number of amino acids: Translated: 878; Mature: 877

Protein sequence:

>878_residues
MTFLSHSREGIAAFILVASQRGEESPMTKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLI
AANILLGDECQVTIEGEDEQVAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLL
SLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDG
TFRQRLLSHIVAGESCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADEL
TPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQ
WLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGA
AKGKPIIIRTIDIGGDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVK
DQLAEVKQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLR
ALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEY
LLVQYDGEQRDEPLIIPECITLGADWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEH
IEQSTISVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL

Sequences:

>Translated_878_residues
MTFLSHSREGIAAFILVASQRGEESPMTKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLI
AANILLGDECQVTIEGEDEQVAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLL
SLIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDG
TFRQRLLSHIVAGESCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADEL
TPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQ
WLHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGA
AKGKPIIIRTIDIGGDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVK
DQLAEVKQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLR
ALDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEY
LLVQYDGEQRDEPLIIPECITLGADWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEH
IEQSTISVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL
>Mature_877_residues
TFLSHSREGIAAFILVASQRGEESPMTKQVTFICGLLNGVHARPASHIERVCNRFQSRFHWYNPRSGIVGDGKSVLSLIA
ANILLGDECQVTIEGEDEQVAFERLSIFIEHELPHADPILPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLS
LIRADLNALGDLPVAQGIEREQQMLADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDGT
FRQRLLSHIVAGESCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGEQRFPSQQALSEDSLCIADELT
PSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLVGVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQW
LHDQLREQQSRYQNMPGRTLDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGAA
KGKPIIIRTIDIGGDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMHGPLKIMIPMISSMEEILWVKD
QLAEVKQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCCEEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLRA
LDYAVCEVHRHGKWIGLCGELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEYL
LVQYDGEQRDEPLIIPECITLGADWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWAREALFSTGLGFGFAIPHAQSEHI
EQSTISVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHMRIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI48994992, Length=848, Percent_Identity=62.5, Blast_Score=1060, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=852, Percent_Identity=41.4319248826291, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1788756, Length=453, Percent_Identity=38.1898454746137, Blast_Score=322, Evalue=7e-89,
Organism=Escherichia coli, GI1789193, Length=509, Percent_Identity=32.6129666011788, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1787994, Length=411, Percent_Identity=26.2773722627737, Blast_Score=97, Evalue=6e-21,
Organism=Escherichia coli, GI1786951, Length=135, Percent_Identity=27.4074074074074, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 97685; Mature: 97554

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00369 PTS_HPR_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLSHSREGIAAFILVASQRGEESPMTKQVTFICGLLNGVHARPASHIERVCNRFQSRF
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
HWYNPRSGIVGDGKSVLSLIAANILLGDECQVTIEGEDEQVAFERLSIFIEHELPHADPI
CCCCCCCCCCCCCHHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
LPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCH
REQQMLADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TFRQRLLSHIVAGESCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
QRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLV
CCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEE
GVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRT
CCCHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGA
CCHHHEEHEECCCEEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
AKGKPIIIRTIDIGGDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMH
CCCCEEEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPLKIMIPMISSMEEILWVKDQLAEVKQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCC
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
EEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCG
HHCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHH
ELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEY
HHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE
LLVQYDGEQRDEPLIIPECITLGADWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWARE
EEEEECCCCCCCCEECHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHM
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHH
RIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFLSHSREGIAAFILVASQRGEESPMTKQVTFICGLLNGVHARPASHIERVCNRFQSRF
CCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
HWYNPRSGIVGDGKSVLSLIAANILLGDECQVTIEGEDEQVAFERLSIFIEHELPHADPI
CCCCCCCCCCCCCHHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
LPKREESAEWEPLPASLAHLHPTLLRARSVSPGTACGKLLSLIRADLNALGDLPVAQGIE
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCH
REQQMLADGVAQLGKAWESLLVANSSTAANSSTTENNSTTENNSTTRAIREVHRSLLRDG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
TFRQRLLSHIVAGESCATAIVATAAYFSQQLALAANTYLRERELDIRDVSFQLLQQIYGE
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
QRFPSQQALSEDSLCIADELTPSQFLALDKRYLKGLLLGRGGSTSHTVILARSFNIPTLV
CCCCCHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEE
GVDAAALQPYINQSLQIDGELGLVVCLLDEPVRRYYRQEQWLHDQLREQQSRYQNMPGRT
CCCHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LDGVRMVVAANITHAVEVEGAFNQGAESIGLFRTEILYMDRAAAPSEEELYTLYAQALGA
CCHHHEEHEECCCEEEEECCCHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
AKGKPIIIRTIDIGGDKPVSYLNIPAESNPFLGYRAVRIYHEFLSLFHTQLRAILRASMH
CCCCEEEEEEEECCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPLKIMIPMISSMEEILWVKDQLAEVKQSLRISHLQFDETVPLGMMLEVPSVMFIIDQCC
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
EEMDFLSIGSNDLTQYLLAVDRDNAKVSEHYHCLPPALLRALDYAVCEVHRHGKWIGLCG
HHCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHH
ELAAKDSVLPLLVAMGLDEISMSASFIGATKARLAKLDRGECRLLLNRVMACRTSREVEY
HHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEE
LLVQYDGEQRDEPLIIPECITLGADWRSKEEVIKGMVDNLLLAGRCRYPRKLAADVWARE
EEEEECCCCCCCCEECHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ALFSTGLGFGFAIPHAQSEHIEQSTISVAKLAKPVNWGEEDALFVMMLTLNKQASGDQHM
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHH
RIFSKLARRIMHEGFRDALVSAGTAQQVEMLLKHELEL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]