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The map label for this gene is rfaQ [H]

Identifier: 22127634

GI number: 22127634

Start: 4184816

End: 4186015

Strand: Reverse

Name: rfaQ [H]

Synonym: y3763

Alternate gene names: 22127634

Gene position: 4186015-4184816 (Counterclockwise)

Preceding gene: 22127635

Following gene: 22127633

Centisome position: 90.99

GC content: 47.25

Gene sequence:

>1200_bases
TTGCCAATGATGGCGCATCCTCTGATGGCGCATCCCATGATAAATACATCAAATAAGACAGCATCACCTGCCGGGCAACT
TTCTGCTCCCGCTTCTCAAACCGGTGCCAGCCACTTGCCGGAAGTACTGCGGAATAAAGAGCATGTTGCGCGCATTTTAG
TGATTAAGTTACGGCATTTCGGCGATGTTCTACTGACGACGCCGCTACTGAGTACGCTGAAAGCCAATTATCCGCAGGCT
AAAATCGATGTACTGCTGTATGGCGGTACGCAGGATATGTTGCGTGAAAACAAGGCGATCAATCATACCTTTATTGTTGA
TCGGGGCCTGAAGCATGCAGGATTAAAGGCGCAAATCAGTGGCGAAAAAGCACTGTTTAGCGCCCTAAAAAAACAACATT
ATGATTTGATTCTTAATTTATCCGATCAATGGCGGGCTGCGGCGTATTGCCGTTTGTTAAAACCCACGTTTAGTATCGGT
TTCCACTACCCAAAACGGGACAATTGGCTCTGGCGTTACTGCCATTCCACATTGGTTGAGATCCCTAATGCTGCAGAACG
GCACACGGTACTCAATAATCTGGATATTCTCTCACCACTACAACTGACCGACATCAGGACCGACGTCACCATGTCCTATG
GGCCGCATGATATCGAGCGGGCAAAGCAGTTGTGCCAACAGCATAATATTGAACACTATATTTTGATTCAGCCTTCCGCC
CGCTGGAAATTCAAAACCTGGGCCAGCGAATCCTTCAGTGAATTGATCAACCACCTCACTCAACAAGGTGAAACTGTTCT
GCTGACGGGCAGTAAAGATCAGGCTGAATTGGATTACATCGCTGAGATTATTGCTGGCTGCACACAACCGGAAAAGGTGA
TCAATTTATCGGGTCAATTGGCATTGCCTGAACTGGCGGTCTTAATTGATAACGCCAAATTATTTATTGGTGTTGATTCT
GTGGCAATGCATATGGCTGCTGCGTTACAGACGCCAGCAGTGGTACTATTTGGCCCGAGTAATCTTAATCAATGGCACCC
GTGGCAGGCTCCACATACTTTACTCTGGGCTGGCAATTACCGCACTTTGCCGCATCCCAATGAAATAGATACTGACACTA
CTGATCGCTATCTGAACGCAATCCCAGTTAGTGATGTCATAGACGCAGTTGATTCCTGGCGAGTTGCCAAAGGGAATTAG

Upstream 100 bases:

>100_bases
ATTCCGCCGGGAGGCTTCTATCAATGACTTTTAACATATTCATATGCTAAAACGATTGCCATGCTGCAAACCCCTGATAT
AGCATAATTGCCCTCTTTCG

Downstream 100 bases:

>100_bases
TGGGTCAGTCGCTTTATTTTTCCTAACCAGCTCGAGCTGAGAAAATTATGACAACTTTATCACCGGCAATTCCGGTCAAT
CGCCGTAGTGAAATGCTGTG

Product: lipopolysaccharide biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MPMMAHPLMAHPMINTSNKTASPAGQLSAPASQTGASHLPEVLRNKEHVARILVIKLRHFGDVLLTTPLLSTLKANYPQA
KIDVLLYGGTQDMLRENKAINHTFIVDRGLKHAGLKAQISGEKALFSALKKQHYDLILNLSDQWRAAAYCRLLKPTFSIG
FHYPKRDNWLWRYCHSTLVEIPNAAERHTVLNNLDILSPLQLTDIRTDVTMSYGPHDIERAKQLCQQHNIEHYILIQPSA
RWKFKTWASESFSELINHLTQQGETVLLTGSKDQAELDYIAEIIAGCTQPEKVINLSGQLALPELAVLIDNAKLFIGVDS
VAMHMAAALQTPAVVLFGPSNLNQWHPWQAPHTLLWAGNYRTLPHPNEIDTDTTDRYLNAIPVSDVIDAVDSWRVAKGN

Sequences:

>Translated_399_residues
MPMMAHPLMAHPMINTSNKTASPAGQLSAPASQTGASHLPEVLRNKEHVARILVIKLRHFGDVLLTTPLLSTLKANYPQA
KIDVLLYGGTQDMLRENKAINHTFIVDRGLKHAGLKAQISGEKALFSALKKQHYDLILNLSDQWRAAAYCRLLKPTFSIG
FHYPKRDNWLWRYCHSTLVEIPNAAERHTVLNNLDILSPLQLTDIRTDVTMSYGPHDIERAKQLCQQHNIEHYILIQPSA
RWKFKTWASESFSELINHLTQQGETVLLTGSKDQAELDYIAEIIAGCTQPEKVINLSGQLALPELAVLIDNAKLFIGVDS
VAMHMAAALQTPAVVLFGPSNLNQWHPWQAPHTLLWAGNYRTLPHPNEIDTDTTDRYLNAIPVSDVIDAVDSWRVAKGN
>Mature_398_residues
PMMAHPLMAHPMINTSNKTASPAGQLSAPASQTGASHLPEVLRNKEHVARILVIKLRHFGDVLLTTPLLSTLKANYPQAK
IDVLLYGGTQDMLRENKAINHTFIVDRGLKHAGLKAQISGEKALFSALKKQHYDLILNLSDQWRAAAYCRLLKPTFSIGF
HYPKRDNWLWRYCHSTLVEIPNAAERHTVLNNLDILSPLQLTDIRTDVTMSYGPHDIERAKQLCQQHNIEHYILIQPSAR
WKFKTWASESFSELINHLTQQGETVLLTGSKDQAELDYIAEIIAGCTQPEKVINLSGQLALPELAVLIDNAKLFIGVDSV
AMHMAAALQTPAVVLFGPSNLNQWHPWQAPHTLLWAGNYRTLPHPNEIDTDTTDRYLNAIPVSDVIDAVDSWRVAKGN

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=336, Percent_Identity=39.8809523809524, Blast_Score=239, Evalue=3e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 44663; Mature: 44532

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMMAHPLMAHPMINTSNKTASPAGQLSAPASQTGASHLPEVLRNKEHVARILVIKLRHF
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
GDVLLTTPLLSTLKANYPQAKIDVLLYGGTQDMLRENKAINHTFIVDRGLKHAGLKAQIS
CHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCCCCEEEEEECCCCCCCCEEEEC
GEKALFSALKKQHYDLILNLSDQWRAAAYCRLLKPTFSIGFHYPKRDNWLWRYCHSTLVE
CHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHH
IPNAAERHTVLNNLDILSPLQLTDIRTDVTMSYGPHDIERAKQLCQQHNIEHYILIQPSA
CCCCHHHHHHHHCCCCCCCCCCEECCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCC
RWKFKTWASESFSELINHLTQQGETVLLTGSKDQAELDYIAEIIAGCTQPEKVINLSGQL
CEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCC
ALPELAVLIDNAKLFIGVDSVAMHMAAALQTPAVVLFGPSNLNQWHPWQAPHTLLWAGNY
CCCEEEEEEECCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEECCC
RTLPHPNEIDTDTTDRYLNAIPVSDVIDAVDSWRVAKGN
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCEECCCC
>Mature Secondary Structure 
PMMAHPLMAHPMINTSNKTASPAGQLSAPASQTGASHLPEVLRNKEHVARILVIKLRHF
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHCHHHHHHHHHHHHHHH
GDVLLTTPLLSTLKANYPQAKIDVLLYGGTQDMLRENKAINHTFIVDRGLKHAGLKAQIS
CHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHCCCCCCEEEEEECCCCCCCCEEEEC
GEKALFSALKKQHYDLILNLSDQWRAAAYCRLLKPTFSIGFHYPKRDNWLWRYCHSTLVE
CHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHH
IPNAAERHTVLNNLDILSPLQLTDIRTDVTMSYGPHDIERAKQLCQQHNIEHYILIQPSA
CCCCHHHHHHHHCCCCCCCCCCEECCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCC
RWKFKTWASESFSELINHLTQQGETVLLTGSKDQAELDYIAEIIAGCTQPEKVINLSGQL
CEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCC
ALPELAVLIDNAKLFIGVDSVAMHMAAALQTPAVVLFGPSNLNQWHPWQAPHTLLWAGNY
CCCEEEEEEECCEEEEEHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEECCC
RTLPHPNEIDTDTTDRYLNAIPVSDVIDAVDSWRVAKGN
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]