Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 22127567

GI number: 22127567

Start: 4105321

End: 4106523

Strand: Reverse

Name: carA [H]

Synonym: y3693

Alternate gene names: 22127567

Gene position: 4106523-4105321 (Counterclockwise)

Preceding gene: 22127568

Following gene: 22127566

Centisome position: 89.26

GC content: 50.46

Gene sequence:

>1203_bases
GTGACTGTTTATTCCCTGGAGGATGTTTTGATTAAGTCAGCGCTATTGGTTCTCGAAGACGGAACCCAATTCCACGGTCG
GGCCATCGGGGCAGAAGGTACGGCAGTAGGGGAAGTGGTCTTCAATACGTCGATGACCGGTTATCAAGAAATCCTCACTG
ATCCTTCCTACTCCCGCCAGATCGTTACTCTTACTTATCCTCATATCGGCAATGTCGGCACTAATGCCTCCGATGAAGAA
TCCTCTGCAGTACACGCCCAAGGTCTTGTTATTCGCGACCTGCCATTGATTGCCAGCAACTACCGTAATGAAGAAGGCTT
ATCCGAGTATCTCAAGCGGCACAATATTGTGGCGATTGCCGATATCGATACTCGTAAGCTGACTCGCTTGCTGCGCGAAA
AAGGAGCACAGAACGGCTGCATTATTGTTGGGGAGTTGTCTGACGCGGCCCTGGCATTGGAAAAAGCGAAAGCATTTCCA
GGCTTGAAAGGGATGGATCTGGCGAAAGAAGTGACCACCAAAGAGATGTATCAGTGGCTGCAAGGCAGTTGGACGCTGGA
AGGTGATTTACCTGCGGCGAAACAACCAGAAGATTTGCCATTCCATGTGGTGGCTTACGATTATGGTGTGAAGCGTAATA
TCCTGCGTATGCTGGTTGATCGCGGTTGCCGTCTGACCGTTGTTCCAGCGCAGACACCCGCTGAAGACGTTCTGAAACTG
AATCCAGATGGCATCTTCTTATCCAATGGTCCTGGGGATCCAGAACCATGCGATTACGCGATCACGGCCATCAAACGCTT
CCTGGAAACGGATATTCCGGTATTCGGCATCTGTCTGGGCCACCAATTGCTGGCGCTGGCCAGTGGCGCTAAAACAGTAA
AAATGAAGTTCGGTCACCATGGGGGTAACCATCCGGTGAAAGATCTGGATGCTAGCTGTGTGATGATTACCGCGCAAAAC
CATGGCTTCGCCGTTGATGAAACCTCATTGCCATCCAACCTGCGTACTACACATGTCTCTTTATTTGATGGTTCTCTGCA
AGGGCTCCACCGTACAGATAAAGCAGCGTTCAGCTTCCAGGGGCATCCAGAAGCCAGCCCCGGCCCGCATGATGCCGCGC
CATTGTTTGATCATTTTATCGAACTGATTGAGGCTTACCGAGCTTCATCCGTGAGTCTTAACTGTAGTAACAGCCATAAA
TAA

Upstream 100 bases:

>100_bases
TTACTCATCATTAAAATGCGCCCAATTTGCCAAAAATTAGCTTTGAGAGCGGTTTTTGCATTGATTTAGATCGCTAGATA
TGAATTAATATGCAGATAAT

Downstream 100 bases:

>100_bases
TCAGGAGCGAATACCATGCCAAAACGTACAGATATAAAAAGCATCCTGATTCTGGGCGCAGGCCCGATTGTTATCGGCCA
GGCTTGTGAGTTTGACTACT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTVYSLEDVLIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEE
SSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFP
GLKGMDLAKEVTTKEMYQWLQGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKL
NPDGIFLSNGPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQN
HGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK

Sequences:

>Translated_400_residues
MTVYSLEDVLIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEE
SSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFP
GLKGMDLAKEVTTKEMYQWLQGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKL
NPDGIFLSNGPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQN
HGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK
>Mature_399_residues
TVYSLEDVLIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNASDEES
SAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPG
LKGMDLAKEVTTKEMYQWLQGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKLN
PDGIFLSNGPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDLDASCVMITAQNH
GFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQGHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=382, Percent_Identity=39.0052356020942, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI169790915, Length=400, Percent_Identity=35.5, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI21361331, Length=400, Percent_Identity=35.5, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1786215, Length=381, Percent_Identity=86.3517060367454, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=390, Percent_Identity=35.1282051282051, Blast_Score=227, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6324878, Length=405, Percent_Identity=38.7654320987654, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=37.0277078085642, Blast_Score=232, Evalue=9e-62,
Organism=Drosophila melanogaster, GI45555749, Length=388, Percent_Identity=39.4329896907216, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24642586, Length=388, Percent_Identity=39.4329896907216, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43443; Mature: 43312

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVYSLEDVLIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQ
CCEECHHHHHHHHHHEEEECCCCCCCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCE
IVTLTYPHIGNVGTNASDEESSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIA
EEEEECCCCCCCCCCCCCCCCCHHHHCCEEEECCCHHHHCCCCHHHHHHHHHHCCEEEEE
DIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAKEVTTKEMYQWL
ECCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKL
CCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHEEE
NPDGIFLSNGPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHH
CCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCC
GGNHPVKDLDASCVMITAQNHGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQ
CCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEEC
GHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure 
TVYSLEDVLIKSALLVLEDGTQFHGRAIGAEGTAVGEVVFNTSMTGYQEILTDPSYSRQ
CEECHHHHHHHHHHEEEECCCCCCCEEECCCCCEEEHEEECCCCCHHHHHHCCCCCCCE
IVTLTYPHIGNVGTNASDEESSAVHAQGLVIRDLPLIASNYRNEEGLSEYLKRHNIVAIA
EEEEECCCCCCCCCCCCCCCCCHHHHCCEEEECCCHHHHCCCCHHHHHHHHHHCCEEEEE
DIDTRKLTRLLREKGAQNGCIIVGELSDAALALEKAKAFPGLKGMDLAKEVTTKEMYQWL
ECCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
QGSWTLEGDLPAAKQPEDLPFHVVAYDYGVKRNILRMLVDRGCRLTVVPAQTPAEDVLKL
CCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCHHHHEEE
NPDGIFLSNGPGDPEPCDYAITAIKRFLETDIPVFGICLGHQLLALASGAKTVKMKFGHH
CCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCEEEEEECCC
GGNHPVKDLDASCVMITAQNHGFAVDETSLPSNLRTTHVSLFDGSLQGLHRTDKAAFSFQ
CCCCCCCCCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCHHHHHHCCCCEEEEC
GHPEASPGPHDAAPLFDHFIELIEAYRASSVSLNCSNSHK
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]