Click here to switch to the map view.

The map label for this gene is polB [H]

Identifier: 22127529

GI number: 22127529

Start: 4055920

End: 4058289

Strand: Direct

Name: polB [H]

Synonym: y3655

Alternate gene names: 22127529

Gene position: 4055920-4058289 (Clockwise)

Preceding gene: 229013123

Following gene: 22127530

Centisome position: 88.16

GC content: 53.12

Gene sequence:

>2370_bases
GTGGGGCAAACCCGTCAGGGCTTCCTGCTGACCCGCCACTGGCGGGATACACCAGCAGGCACCGAAGTCGAATTCTGGTT
GGCAACGGATGAAGGGCCACAGCATGTCAGGCTGCCGCCCCAGCCATCGGTCGCCTTTATCCCTGCCGAACAACAGCAAC
GGGCAGAAACCGTACTACGCGCAGAACGCCATTGGCAACTGCGCCCTCTCGCGCTGACTGATTTTCACCAACGCCCGGTT
CTTGGCCTGTATTGCACTCAGCACCGCCAGTTGATCCGCCTGGAAAAACGACTGCGCGAAAGTGGCGTGAATGTTTACGA
AGGTGATATTCGCCCGCCAGAACGCTTTCTGATGGAGCGGTTTATTACTGCCCCCGTATGGTTTAGTGGGCAGGAAAATG
ACCATGGCCCACTCCTCAATACACAACTAAAACCGGCTGATGACTATCGCCCAACCCTCAAACTGGTTTCTCTGGATATC
GAAACCAGTGAACACGGCGAGCTGTACTGTATTGGGCTGGAAGGCTGTGGGCAACGGCAAGTCTATATGTTAGGGCCACC
CAATGGGGAGACCACGGGCACCCCGCTCGATTTCAATTTGGAATACGTTGCCAGCCGCCCATTACTATTAGAAAAACTCA
ACCAATGGCTGGCAGTGCATGATCCTGATGCCATTATTGGCTGGAATCTGGTGCAGTTCGATTTACGGGTATTACAGAAA
CACGCAGATCGCTACCAGATCCCGCTGCGCTTTGGCCGTGGCGGTAACCTGCTCGAGTGGCGTGAACACGGCTTTAAGCA
AGGGCATTTCTTTGCTTCCGCCGCCGGGCGTTTAATTATTGATGGTATTGAAGCACTGAAATCCGCCACGTGGAACTTCC
CTTCTTTCAGTCTGGAATCTGTCTCGCAAACTTTACTTGGCGAGGGTAAAGCCAGCGATAGCCCCTATCAGCGGATGGAT
GAAATTAATCAGCGCTTCGCCCACGATAAACCAGCACTGGCCCGTTATAACCTGAAAGACTGTGAGTTGGTCACGCGGAT
TTTTGCCAAAACGGAGTTGCTGAGTTTCTTACTTGAACGCTCAACCGTGACCGGGCTGGCAGCCGATCGTAGTGGCGGTT
CGGTGGCCGCGTTTACCCACCTTTATCTGCCACGGATGCACCGTATCGGCTATGTTGCCCCCAATTTAGGTGAATTGCCG
GAAGAGCACAGCCCCGGTGGTTTTGTAATGGATTCCCAGCCCGGTTTGTATGATTCAGTGCTGGTACTTGACTATAAAAG
CCTGTACCCATCGATTATCCGCACCTTTTTAATCGATCCGGTCGGTCTGGTGGCGGGGATGCAGCAACCTGATATTCAGC
ACTCGGTACCGGGGTTTCGTGGGGCCTGGTTCTCACGCGAGAAACATTGTCTGCCCGCCATTGTTAACCAAATCTGGCAA
GGGCGGGAAGCAGCGAAACGCCAGCAAAATAAGCCGCTGTCACAGGCGCTGAAAATCATTATGAATGCCTTTTATGGCGT
GCTGGGTTCCAGCGGCTGCCGTTTTTTCGATCCTCGGCTGGCATCATCCATTACCCTGCGTGGTCATGAGATCATGCGAC
AAACCCGTGAGTTAATTGAAGCGCAGGGTTATCAGGTGATTTATGGTGACACCGATTCAACCTTTGTTTGGCTGAAAAGC
GCCCACAACGATGAGCAAGCAACACGCATCGGTAACGAGCTGGTGCAGTTAGTGAATCAATGGTGGCAAAACCATATACA
GCAAAACTTTAATCTGCCCTGTGCATTAGAGCTGGAGTTCGAAATCCACTACCGGCGTTTTTTGATGCCCACTATTCGGG
GCGCGGAGCAAGGCAGTAAAAAACGCTATGCGGGACTCATCTCCACCCCGACGGGTGATCAAATGGTTTATAAAGGGCTG
GAAACCGTCCGTACCGATTGGACCCCACTGGCACAGCAATTCCAGCGCGAGCTGTATCTGCGTATCTTCCAACAACAACC
GTATCAAGATTATGTGCGGGATTACGTAGCCCGTACCCTGAATGGCGAGTTGGATGAGCAACTGGTTTACCGCAAACGCC
TGCGTCGGCGGTTGGATGACTATCAGCGCAACGTCCCCCCGCACGCCAGAGCGGCACGTCTGGCCGATGAGTTTAACCGT
AAGCTTGGCCGGCCACTGCAATATCAAAATGGCGGGTGGATCAGCTATGTGATGACCACCGCAGGCCCCGAACCTTTAGA
AACGCGGCAATCGCCCATCGATTATGATCACTATCTGACACGCCAGCTACAACCCGTAGCAGACGCTATACTTCCCTTCA
CTCAGGATGATTTCACCACACTGATCACCGGTCAGATGGGACTTTTTTAA

Upstream 100 bases:

>100_bases
AATTTGCTATTGAAATAGACAATAACTGTATAAAAACCCATACTAATACAGTATAAATCTTCAAACGTTATTCATTCACA
TCTTAATTTAAGGTAGCAGC

Downstream 100 bases:

>100_bases
TGATAATAGGGTTGTTTTAGAGGTGACGAAGCGCTCGCCTTCCTTTACCATAGCGCCCTTGCCAAAATTTGTATCAATCA
CATTTACATAACCGACTGAT

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MGQTRQGFLLTRHWRDTPAGTEVEFWLATDEGPQHVRLPPQPSVAFIPAEQQQRAETVLRAERHWQLRPLALTDFHQRPV
LGLYCTQHRQLIRLEKRLRESGVNVYEGDIRPPERFLMERFITAPVWFSGQENDHGPLLNTQLKPADDYRPTLKLVSLDI
ETSEHGELYCIGLEGCGQRQVYMLGPPNGETTGTPLDFNLEYVASRPLLLEKLNQWLAVHDPDAIIGWNLVQFDLRVLQK
HADRYQIPLRFGRGGNLLEWREHGFKQGHFFASAAGRLIIDGIEALKSATWNFPSFSLESVSQTLLGEGKASDSPYQRMD
EINQRFAHDKPALARYNLKDCELVTRIFAKTELLSFLLERSTVTGLAADRSGGSVAAFTHLYLPRMHRIGYVAPNLGELP
EEHSPGGFVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVAGMQQPDIQHSVPGFRGAWFSREKHCLPAIVNQIWQ
GREAAKRQQNKPLSQALKIIMNAFYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQGYQVIYGDTDSTFVWLKS
AHNDEQATRIGNELVQLVNQWWQNHIQQNFNLPCALELEFEIHYRRFLMPTIRGAEQGSKKRYAGLISTPTGDQMVYKGL
ETVRTDWTPLAQQFQRELYLRIFQQQPYQDYVRDYVARTLNGELDEQLVYRKRLRRRLDDYQRNVPPHARAARLADEFNR
KLGRPLQYQNGGWISYVMTTAGPEPLETRQSPIDYDHYLTRQLQPVADAILPFTQDDFTTLITGQMGLF

Sequences:

>Translated_789_residues
MGQTRQGFLLTRHWRDTPAGTEVEFWLATDEGPQHVRLPPQPSVAFIPAEQQQRAETVLRAERHWQLRPLALTDFHQRPV
LGLYCTQHRQLIRLEKRLRESGVNVYEGDIRPPERFLMERFITAPVWFSGQENDHGPLLNTQLKPADDYRPTLKLVSLDI
ETSEHGELYCIGLEGCGQRQVYMLGPPNGETTGTPLDFNLEYVASRPLLLEKLNQWLAVHDPDAIIGWNLVQFDLRVLQK
HADRYQIPLRFGRGGNLLEWREHGFKQGHFFASAAGRLIIDGIEALKSATWNFPSFSLESVSQTLLGEGKASDSPYQRMD
EINQRFAHDKPALARYNLKDCELVTRIFAKTELLSFLLERSTVTGLAADRSGGSVAAFTHLYLPRMHRIGYVAPNLGELP
EEHSPGGFVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVAGMQQPDIQHSVPGFRGAWFSREKHCLPAIVNQIWQ
GREAAKRQQNKPLSQALKIIMNAFYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQGYQVIYGDTDSTFVWLKS
AHNDEQATRIGNELVQLVNQWWQNHIQQNFNLPCALELEFEIHYRRFLMPTIRGAEQGSKKRYAGLISTPTGDQMVYKGL
ETVRTDWTPLAQQFQRELYLRIFQQQPYQDYVRDYVARTLNGELDEQLVYRKRLRRRLDDYQRNVPPHARAARLADEFNR
KLGRPLQYQNGGWISYVMTTAGPEPLETRQSPIDYDHYLTRQLQPVADAILPFTQDDFTTLITGQMGLF
>Mature_788_residues
GQTRQGFLLTRHWRDTPAGTEVEFWLATDEGPQHVRLPPQPSVAFIPAEQQQRAETVLRAERHWQLRPLALTDFHQRPVL
GLYCTQHRQLIRLEKRLRESGVNVYEGDIRPPERFLMERFITAPVWFSGQENDHGPLLNTQLKPADDYRPTLKLVSLDIE
TSEHGELYCIGLEGCGQRQVYMLGPPNGETTGTPLDFNLEYVASRPLLLEKLNQWLAVHDPDAIIGWNLVQFDLRVLQKH
ADRYQIPLRFGRGGNLLEWREHGFKQGHFFASAAGRLIIDGIEALKSATWNFPSFSLESVSQTLLGEGKASDSPYQRMDE
INQRFAHDKPALARYNLKDCELVTRIFAKTELLSFLLERSTVTGLAADRSGGSVAAFTHLYLPRMHRIGYVAPNLGELPE
EHSPGGFVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVAGMQQPDIQHSVPGFRGAWFSREKHCLPAIVNQIWQG
REAAKRQQNKPLSQALKIIMNAFYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIEAQGYQVIYGDTDSTFVWLKSA
HNDEQATRIGNELVQLVNQWWQNHIQQNFNLPCALELEFEIHYRRFLMPTIRGAEQGSKKRYAGLISTPTGDQMVYKGLE
TVRTDWTPLAQQFQRELYLRIFQQQPYQDYVRDYVARTLNGELDEQLVYRKRLRRRLDDYQRNVPPHARAARLADEFNRK
LGRPLQYQNGGWISYVMTTAGPEPLETRQSPIDYDHYLTRQLQPVADAILPFTQDDFTTLITGQMGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI106507301, Length=647, Percent_Identity=26.2751159196291, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI156616275, Length=648, Percent_Identity=27.0061728395062, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1786246, Length=783, Percent_Identity=72.4137931034483, Blast_Score=1172, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32565317, Length=790, Percent_Identity=23.7974683544304, Blast_Score=139, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17559488, Length=662, Percent_Identity=26.4350453172205, Blast_Score=136, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI86563326, Length=351, Percent_Identity=27.6353276353276, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320101, Length=636, Percent_Identity=25.4716981132075, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324227, Length=430, Percent_Identity=28.3720930232558, Blast_Score=114, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24664937, Length=640, Percent_Identity=25.3125, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90559; Mature: 90428

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQTRQGFLLTRHWRDTPAGTEVEFWLATDEGPQHVRLPPQPSVAFIPAEQQQRAETVLR
CCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEECCCCCCEEEECCHHHHHHHHHHH
AERHWQLRPLALTDFHQRPVLGLYCTQHRQLIRLEKRLRESGVNVYEGDIRPPERFLMER
HHHCCCCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHH
FITAPVWFSGQENDHGPLLNTQLKPADDYRPTLKLVSLDIETSEHGELYCIGLEGCGQRQ
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCEE
VYMLGPPNGETTGTPLDFNLEYVASRPLLLEKLNQWLAVHDPDAIIGWNLVQFDLRVLQK
EEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHEEECCCCCEECCHHHHHHHHHHHH
HADRYQIPLRFGRGGNLLEWREHGFKQGHFFASAAGRLIIDGIEALKSATWNFPSFSLES
HCCCEEEEEEECCCCCCHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCHHH
VSQTLLGEGKASDSPYQRMDEINQRFAHDKPALARYNLKDCELVTRIFAKTELLSFLLER
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHH
STVTGLAADRSGGSVAAFTHLYLPRMHRIGYVAPNLGELPEEHSPGGFVMDSQPGLYDSV
HHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHH
LVLDYKSLYPSIIRTFLIDPVGLVAGMQQPDIQHSVPGFRGAWFSREKHCLPAIVNQIWQ
HEEEHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHC
GREAAKRQQNKPLSQALKIIMNAFYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIE
CHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHEEECHHHHHHHHHHHHH
AQGYQVIYGDTDSTFVWLKSAHNDEQATRIGNELVQLVNQWWQNHIQQNFNLPCALELEF
HCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EIHYRRFLMPTIRGAEQGSKKRYAGLISTPTGDQMVYKGLETVRTDWTPLAQQFQRELYL
EEHHHHHHHHHHCCCCCCCCHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RIFQQQPYQDYVRDYVARTLNGELDEQLVYRKRLRRRLDDYQRNVPPHARAARLADEFNR
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KLGRPLQYQNGGWISYVMTTAGPEPLETRQSPIDYDHYLTRQLQPVADAILPFTQDDFTT
HHCCCCEECCCCEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHE
LITGQMGLF
EEECCCCCC
>Mature Secondary Structure 
GQTRQGFLLTRHWRDTPAGTEVEFWLATDEGPQHVRLPPQPSVAFIPAEQQQRAETVLR
CCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCEEECCCCCCEEEECCHHHHHHHHHHH
AERHWQLRPLALTDFHQRPVLGLYCTQHRQLIRLEKRLRESGVNVYEGDIRPPERFLMER
HHHCCCCCCEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHH
FITAPVWFSGQENDHGPLLNTQLKPADDYRPTLKLVSLDIETSEHGELYCIGLEGCGQRQ
HHCCCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCEE
VYMLGPPNGETTGTPLDFNLEYVASRPLLLEKLNQWLAVHDPDAIIGWNLVQFDLRVLQK
EEEEECCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHEEECCCCCEECCHHHHHHHHHHHH
HADRYQIPLRFGRGGNLLEWREHGFKQGHFFASAAGRLIIDGIEALKSATWNFPSFSLES
HCCCEEEEEEECCCCCCHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCCHHH
VSQTLLGEGKASDSPYQRMDEINQRFAHDKPALARYNLKDCELVTRIFAKTELLSFLLER
HHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHH
STVTGLAADRSGGSVAAFTHLYLPRMHRIGYVAPNLGELPEEHSPGGFVMDSQPGLYDSV
HHHHEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHH
LVLDYKSLYPSIIRTFLIDPVGLVAGMQQPDIQHSVPGFRGAWFSREKHCLPAIVNQIWQ
HEEEHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCHHHHHHHHHHC
GREAAKRQQNKPLSQALKIIMNAFYGVLGSSGCRFFDPRLASSITLRGHEIMRQTRELIE
CHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHEEECHHHHHHHHHHHHH
AQGYQVIYGDTDSTFVWLKSAHNDEQATRIGNELVQLVNQWWQNHIQQNFNLPCALELEF
HCCCEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
EIHYRRFLMPTIRGAEQGSKKRYAGLISTPTGDQMVYKGLETVRTDWTPLAQQFQRELYL
EEHHHHHHHHHHCCCCCCCCHHHCCEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
RIFQQQPYQDYVRDYVARTLNGELDEQLVYRKRLRRRLDDYQRNVPPHARAARLADEFNR
HHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KLGRPLQYQNGGWISYVMTTAGPEPLETRQSPIDYDHYLTRQLQPVADAILPFTQDDFTT
HHCCCCEECCCCEEEEEEECCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHE
LITGQMGLF
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]