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The map label for this gene is exuT [H]

Identifier: 22127475

GI number: 22127475

Start: 3998477

End: 3999781

Strand: Direct

Name: exuT [H]

Synonym: y3601

Alternate gene names: 22127475

Gene position: 3998477-3999781 (Clockwise)

Preceding gene: 22127470

Following gene: 161484750

Centisome position: 86.91

GC content: 51.34

Gene sequence:

>1305_bases
ATGATGCGTAAAATTAAAGGGTTACGCTGGTACATGATCGCATTGGTCACCGTCGGTACCATATTAGGTTACTTAACACG
TAATGCCATTGCTGTTGCCGCGCCGACACTGCAAGAACAGTTACATATCACCACCCAGCAATATTCTTATATTATCGCTG
CTTATTCAGCCGCTTATACCCTAATGCAACCAGTAGCCGGTTATGTATTGGACGTTATGGGTACCAAAGTGGGCTATGCC
ATGTTTGCCGTTATGTGGGCCATATTTTGTATGAGTACCGCCTTGGCCAGCAGTTGGGGTGGATTAGCTATCGCCCGTGG
TGCGGTAGGTATGGCAGAGGCGGCTATGATCCCTGCAGGCCTGAAAGCCAGTAGTGAATGGTTCCCGGCCAAAGAGCGTT
CTATTGCGGTAGGCTACTTTAACGTTGGCTCCTCCATCGGCGGTATGATTGCTCCCCCTCTCGTGGTCTGGGCTATCGTG
ATGCACAGTTGGCAGATGGCGTTCATCATTACCGGTGTACTCAGCCTGATTTGGGCCATTGCCTGGTTAATCCTCTACAA
ACACCCGAAAGATCAAAAAAGACTCAGCGACGAAGAACGTGAGTACATCCTTAGCGGCCAAGAAGCACAGCACTCCACTG
CTAATGCCAAAAAGATGTCTGCGATGCAGATTATCCGCAACCGTCAATTCTGGGGTATTGCCATCCCGCGTTTCTTGGCA
GAACCCGCTTGGGGGACGTTCAACGCGTGGATTCCGCTCTTCATGTTTAAAGCTTATGGCTTTAACTTGAAAGAGATAGC
CATGTTTGCCTGGATGCCAATGCTGTTTGCTGATGTAGGCTGCATTCTGGGTGGCTACCTGCCGCCACTGTTCCAAAAAT
ACTTTAAGGTTAACCTGATTGTTTCCCGTAAGTTAGTCGTCACTATGGGTGGGGTATTGATGATTGGGCCTGGGACCATC
GGCCTATTCACCAGCCCTTATGCGGCTATCGCGCTGCTCTGTATCGGGGGCTTTGCTCACCAAGCACTGTCAGGAGCACT
GATTACATTATCTTCTGACGTTTTTGGCCGTAACGAAGTCGCAACCGCCAACGGCCTGACGGGGATGGCGGCCTGGACGG
CCAGTACTCTGTTCGCCCTCGTGGTAGGGGCATTAGCCGATACCATGGGCTTCAGCCCACTGTTTGCTGCACTGGCGGTA
TTTGATTTACTGGCCGTCGTGGTTATCTGGACCGTGTTACAAGATCGCACAGCAGATGAACCAACGAACGACCCGGTTCA
ACGGCCGCCGGTTGGACAAAACTGA

Upstream 100 bases:

>100_bases
CCGCATGCGCAGCATTGCCGGGTTGGACCAAATTGTGCCATGGAACGCCACCATCAACTGCGTTTCATTGCAGTCATCTT
AGACTGGGAGAAAGTTACAC

Downstream 100 bases:

>100_bases
CCTTACTCTCTGTAATTAAATGACCTGCCTGTGATTAAATGACCTACTAACATGAAAGCCCGGTAAATTTATCGGGCTTT
CATCTCTCCTGTTATCCGCA

Product: transport of hexuronates

Products: galacturonate [Cytoplasm]; Proton [Cytoplasm]; glucuronate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MMRKIKGLRWYMIALVTVGTILGYLTRNAIAVAAPTLQEQLHITTQQYSYIIAAYSAAYTLMQPVAGYVLDVMGTKVGYA
MFAVMWAIFCMSTALASSWGGLAIARGAVGMAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGGMIAPPLVVWAIV
MHSWQMAFIITGVLSLIWAIAWLILYKHPKDQKRLSDEEREYILSGQEAQHSTANAKKMSAMQIIRNRQFWGIAIPRFLA
EPAWGTFNAWIPLFMFKAYGFNLKEIAMFAWMPMLFADVGCILGGYLPPLFQKYFKVNLIVSRKLVVTMGGVLMIGPGTI
GLFTSPYAAIALLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMAAWTASTLFALVVGALADTMGFSPLFAALAV
FDLLAVVVIWTVLQDRTADEPTNDPVQRPPVGQN

Sequences:

>Translated_434_residues
MMRKIKGLRWYMIALVTVGTILGYLTRNAIAVAAPTLQEQLHITTQQYSYIIAAYSAAYTLMQPVAGYVLDVMGTKVGYA
MFAVMWAIFCMSTALASSWGGLAIARGAVGMAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGGMIAPPLVVWAIV
MHSWQMAFIITGVLSLIWAIAWLILYKHPKDQKRLSDEEREYILSGQEAQHSTANAKKMSAMQIIRNRQFWGIAIPRFLA
EPAWGTFNAWIPLFMFKAYGFNLKEIAMFAWMPMLFADVGCILGGYLPPLFQKYFKVNLIVSRKLVVTMGGVLMIGPGTI
GLFTSPYAAIALLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMAAWTASTLFALVVGALADTMGFSPLFAALAV
FDLLAVVVIWTVLQDRTADEPTNDPVQRPPVGQN
>Mature_434_residues
MMRKIKGLRWYMIALVTVGTILGYLTRNAIAVAAPTLQEQLHITTQQYSYIIAAYSAAYTLMQPVAGYVLDVMGTKVGYA
MFAVMWAIFCMSTALASSWGGLAIARGAVGMAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGGMIAPPLVVWAIV
MHSWQMAFIITGVLSLIWAIAWLILYKHPKDQKRLSDEEREYILSGQEAQHSTANAKKMSAMQIIRNRQFWGIAIPRFLA
EPAWGTFNAWIPLFMFKAYGFNLKEIAMFAWMPMLFADVGCILGGYLPPLFQKYFKVNLIVSRKLVVTMGGVLMIGPGTI
GLFTSPYAAIALLCIGGFAHQALSGALITLSSDVFGRNEVATANGLTGMAAWTASTLFALVVGALADTMGFSPLFAALAV
FDLLAVVVIWTVLQDRTADEPTNDPVQRPPVGQN

Specific function: Aldohexuronate transport system [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Phthalate permease family [H]

Homologues:

Organism=Homo sapiens, GI6912666, Length=342, Percent_Identity=24.8538011695906, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI148743791, Length=276, Percent_Identity=25.3623188405797, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI154800443, Length=383, Percent_Identity=24.0208877284595, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367193, Length=428, Percent_Identity=84.1121495327103, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI87082320, Length=394, Percent_Identity=26.3959390862944, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI2367379, Length=313, Percent_Identity=27.4760383386581, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789515, Length=406, Percent_Identity=24.1379310344828, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1789152, Length=328, Percent_Identity=24.0853658536585, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87082071, Length=378, Percent_Identity=20.8994708994709, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17552542, Length=308, Percent_Identity=23.3766233766234, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321698, Length=245, Percent_Identity=21.6326530612245, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24641682, Length=166, Percent_Identity=32.5301204819277, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24580790, Length=290, Percent_Identity=25.8620689655172, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI20129235, Length=404, Percent_Identity=23.7623762376238, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24639764, Length=370, Percent_Identity=21.8918918918919, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI45550460, Length=416, Percent_Identity=25.2403846153846, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24655255, Length=364, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28573299, Length=402, Percent_Identity=23.6318407960199, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653727, Length=418, Percent_Identity=23.444976076555, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004744
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47142; Mature: 47142

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRKIKGLRWYMIALVTVGTILGYLTRNAIAVAAPTLQEQLHITTQQYSYIIAAYSAAYT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LMQPVAGYVLDVMGTKVGYAMFAVMWAIFCMSTALASSWGGLAIARGAVGMAEAAMIPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHCCCC
LKASSEWFPAKERSIAVGYFNVGSSIGGMIAPPLVVWAIVMHSWQMAFIITGVLSLIWAI
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLILYKHPKDQKRLSDEEREYILSGQEAQHSTANAKKMSAMQIIRNRQFWGIAIPRFLA
HHHHHHCCCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHC
EPAWGTFNAWIPLFMFKAYGFNLKEIAMFAWMPMLFADVGCILGGYLPPLFQKYFKVNLI
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VSRKLVVTMGGVLMIGPGTIGLFTSPYAAIALLCIGGFAHQALSGALITLSSDVFGRNEV
HHHHHHEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
ATANGLTGMAAWTASTLFALVVGALADTMGFSPLFAALAVFDLLAVVVIWTVLQDRTADE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTNDPVQRPPVGQN
CCCCCCCCCCCCCC
>Mature Secondary Structure
MMRKIKGLRWYMIALVTVGTILGYLTRNAIAVAAPTLQEQLHITTQQYSYIIAAYSAAYT
CCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHH
LMQPVAGYVLDVMGTKVGYAMFAVMWAIFCMSTALASSWGGLAIARGAVGMAEAAMIPAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHCCCC
LKASSEWFPAKERSIAVGYFNVGSSIGGMIAPPLVVWAIVMHSWQMAFIITGVLSLIWAI
CCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWLILYKHPKDQKRLSDEEREYILSGQEAQHSTANAKKMSAMQIIRNRQFWGIAIPRFLA
HHHHHHCCCCHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHC
EPAWGTFNAWIPLFMFKAYGFNLKEIAMFAWMPMLFADVGCILGGYLPPLFQKYFKVNLI
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VSRKLVVTMGGVLMIGPGTIGLFTSPYAAIALLCIGGFAHQALSGALITLSSDVFGRNEV
HHHHHHEEECCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCC
ATANGLTGMAAWTASTLFALVVGALADTMGFSPLFAALAVFDLLAVVVIWTVLQDRTADE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PTNDPVQRPPVGQN
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: galacturonate [Periplasm]; Proton [Periplasm]; glucuronate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + galacturonate [Periplasm] = galacturonate [Cytoplasm] + Proton [Cytoplasm] Proton [Periplasm] + glucuronate [Periplasm] = Proton [Cytoplasm] + glucuronate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]