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The map label for this gene is dnaG [H]

Identifier: 22127410

GI number: 22127410

Start: 3919542

End: 3921290

Strand: Direct

Name: dnaG [H]

Synonym: y3536

Alternate gene names: 22127410

Gene position: 3919542-3921290 (Clockwise)

Preceding gene: 22127409

Following gene: 22127411

Centisome position: 85.19

GC content: 48.08

Gene sequence:

>1749_bases
ATGGCTGGACGAATTCCACGTGTATTTATCAATGACTTGCTAGCTCGCACTGACATCATCGATCTTATCGATGCTAGGGT
AAAGCTGAAGAAGCAAGGCAAAAATTACCATGCGTGCTGTCCGTTCCATCATGAGAAAACACCCTCATTCACTGTTAACG
GCGAAAAACAGTTTTATCACTGTTTTGGCTGCGGCGCTCATGGCAATGCTGTTGATTTTTTGATGAATTACGATCGACTC
GAGTTTGTCGAAAGTATCGAAGAATTAGCCACCATGCACGGGCTGGAAGTGCCTTACGAGGCAGGCAGCGGCACCACTCA
GATCGAACGACACCAACGACAAAGTCTTTATCAATTGATGGAAAGCCTAAGCGCATTCTATCAGCAGTCACTCAAAGGCC
AGAATGCCAAACAGGCTCGTGAATATCTTAAACATCGCGGTTTGAGTGAAGAAATCATCCAACATTTTGCTATCGGTTTT
GCGCCCCCTGGTTGGGACAATGCCTTAAAACGTTTTGGTCGTGATGGCGAGAGCCGTACAGCACTGAACGATGCCGGGAT
GCTGGTAACTAATGATACCGGGCGGACTTACGATCGTTTTCGTGAGCGCGTCATGTTCCCTATTCGCGATAAACGCGGGC
GGGTCATCGCTTTTGGCGGGCGAATTCTGGGTGATGGAGTGCCGAAGTACCTCAACTCCCCAGAAACAGAAATTTTTCAT
AAAGGCCGCCAGTTATATGGCTTGTATGAAGCACAAGTCAATCATCCAAACCCAACCCGCCTACTCGTTGTGGAAGGGTA
TATGGATGTGGTGGCACTCGCACAATTTGGTATTGATTATGCTGTTGCCTCATTGGGAACAGCGACAACCGCTGAGCATA
TTCAGCTGTTATTCCGCGCCACCGATAACGTAATTTGTTGTTATGACGGTGATCGCGCTGGTAGAGATGCCGCCTGGCGA
GCATTAGAAACGGCACTGCCCTATCTAAATGACGGACGTCAGCTACGCTTTATGTTTTTGCCCGATGGCGAAGACCCTGA
CACCTTGGTTCGTAAAGAAGGGAAAGACGCATTTGAACAACGGATGGAGGCGGCACAGCCACTCTCAACCTTCTTGTTTG
AAACGTTAATGCCACAGGTAGATTTGAGCAGCCCTGATGGGCGCGCCAAACTGAGCACACTGGCATTACCTTTAATCAGC
CAAGTACCTGGTGAAGCGCTGCGCTTGTACCTACGTCAGCAATTAGGGAATAAGTTAGGTCTGCTTGATGACAGCCAGCT
CGATAAGCTGATGCCAAAACAGATTGATAATGCAAATACCTACCAGCCGCCCCAGCTAAAACGCACAACCATGCGTATAC
TTATAGGGCTATTGGTACAAAACCCACAATTGGCTACACTTATTCCCTCATTACAGGGGCTCGAACAAGCCAAATTGGCG
GGTTTACCGTTATTTATTGAGTTGGTTGAAACCTGTCTGGCCCAACCGGGGCTGACAACCGGGCAGTTGCTGGAACTGTA
CCGTGATAATAAATTCAGCCAACAACTTGAAACTCTTGCAACATGGAACCACATGATTGTAGAGGATATGGTCGAACCGA
CTTTTGTCGACACGCTAGCGAGCCTGTATGACTCCATTTTGGAGCAACGTCAGGAAACGCTAATAGCGCGGGACCGTACA
CATGGATTAAACGCCGAAGAACGTAAAGAGCTTTGGTCTTTGAATCTGGCACTAGCCAGAAAAAAATGA

Upstream 100 bases:

>100_bases
TTTCGAAGAACGCGCAGATCGAGTAGTTGCATACGAAGGCCGTGCTTTCCGAAAGGAATGCGCGGCTTGTTCTCGTTTAT
GGACTGGCGAATAGGGGCTT

Downstream 100 bases:

>100_bases
AACACCCAACGGCTTAATTGCCGATAAATGTGGGGGCAACTCCCCACAATATGCCGCTATAGCGGGCAGCGGCGACAAAA
AAAGGCCCCAAATGTAATTG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAGRIPRVFINDLLARTDIIDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYHCFGCGAHGNAVDFLMNYDRL
EFVESIEELATMHGLEVPYEAGSGTTQIERHQRQSLYQLMESLSAFYQQSLKGQNAKQAREYLKHRGLSEEIIQHFAIGF
APPGWDNALKRFGRDGESRTALNDAGMLVTNDTGRTYDRFRERVMFPIRDKRGRVIAFGGRILGDGVPKYLNSPETEIFH
KGRQLYGLYEAQVNHPNPTRLLVVEGYMDVVALAQFGIDYAVASLGTATTAEHIQLLFRATDNVICCYDGDRAGRDAAWR
ALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKDAFEQRMEAAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIS
QVPGEALRLYLRQQLGNKLGLLDDSQLDKLMPKQIDNANTYQPPQLKRTTMRILIGLLVQNPQLATLIPSLQGLEQAKLA
GLPLFIELVETCLAQPGLTTGQLLELYRDNKFSQQLETLATWNHMIVEDMVEPTFVDTLASLYDSILEQRQETLIARDRT
HGLNAEERKELWSLNLALARKK

Sequences:

>Translated_582_residues
MAGRIPRVFINDLLARTDIIDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYHCFGCGAHGNAVDFLMNYDRL
EFVESIEELATMHGLEVPYEAGSGTTQIERHQRQSLYQLMESLSAFYQQSLKGQNAKQAREYLKHRGLSEEIIQHFAIGF
APPGWDNALKRFGRDGESRTALNDAGMLVTNDTGRTYDRFRERVMFPIRDKRGRVIAFGGRILGDGVPKYLNSPETEIFH
KGRQLYGLYEAQVNHPNPTRLLVVEGYMDVVALAQFGIDYAVASLGTATTAEHIQLLFRATDNVICCYDGDRAGRDAAWR
ALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKDAFEQRMEAAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIS
QVPGEALRLYLRQQLGNKLGLLDDSQLDKLMPKQIDNANTYQPPQLKRTTMRILIGLLVQNPQLATLIPSLQGLEQAKLA
GLPLFIELVETCLAQPGLTTGQLLELYRDNKFSQQLETLATWNHMIVEDMVEPTFVDTLASLYDSILEQRQETLIARDRT
HGLNAEERKELWSLNLALARKK
>Mature_581_residues
AGRIPRVFINDLLARTDIIDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYHCFGCGAHGNAVDFLMNYDRLE
FVESIEELATMHGLEVPYEAGSGTTQIERHQRQSLYQLMESLSAFYQQSLKGQNAKQAREYLKHRGLSEEIIQHFAIGFA
PPGWDNALKRFGRDGESRTALNDAGMLVTNDTGRTYDRFRERVMFPIRDKRGRVIAFGGRILGDGVPKYLNSPETEIFHK
GRQLYGLYEAQVNHPNPTRLLVVEGYMDVVALAQFGIDYAVASLGTATTAEHIQLLFRATDNVICCYDGDRAGRDAAWRA
LETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKDAFEQRMEAAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLISQ
VPGEALRLYLRQQLGNKLGLLDDSQLDKLMPKQIDNANTYQPPQLKRTTMRILIGLLVQNPQLATLIPSLQGLEQAKLAG
LPLFIELVETCLAQPGLTTGQLLELYRDNKFSQQLETLATWNHMIVEDMVEPTFVDTLASLYDSILEQRQETLIARDRTH
GLNAEERKELWSLNLALARKK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=581, Percent_Identity=76.7641996557659, Blast_Score=944, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65776; Mature: 65645

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPRVFINDLLARTDIIDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCEEEE
CFGCGAHGNAVDFLMNYDRLEFVESIEELATMHGLEVPYEAGSGTTQIERHQRQSLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
ESLSAFYQQSLKGQNAKQAREYLKHRGLSEEIIQHFAIGFAPPGWDNALKRFGRDGESRT
HHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCC
ALNDAGMLVTNDTGRTYDRFRERVMFPIRDKRGRVIAFGGRILGDGVPKYLNSPETEIFH
CCCCCCEEEECCCCCHHHHHHHHCCCCEECCCCCEEEECCCHHCCCCHHHCCCCHHHHHH
KGRQLYGLYEAQVNHPNPTRLLVVEGYMDVVALAQFGIDYAVASLGTATTAEHIQLLFRA
CCHHHEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TDNVICCYDGDRAGRDAAWRALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKDAFEQ
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHH
RMEAAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLISQVPGEALRLYLRQQLGNKLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
LLDDSQLDKLMPKQIDNANTYQPPQLKRTTMRILIGLLVQNPQLATLIPSLQGLEQAKLA
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHC
GLPLFIELVETCLAQPGLTTGQLLELYRDNKFSQQLETLATWNHMIVEDMVEPTFVDTLA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SLYDSILEQRQETLIARDRTHGLNAEERKELWSLNLALARKK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AGRIPRVFINDLLARTDIIDLIDARVKLKKQGKNYHACCPFHHEKTPSFTVNGEKQFYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCEEEE
CFGCGAHGNAVDFLMNYDRLEFVESIEELATMHGLEVPYEAGSGTTQIERHQRQSLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
ESLSAFYQQSLKGQNAKQAREYLKHRGLSEEIIQHFAIGFAPPGWDNALKRFGRDGESRT
HHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCC
ALNDAGMLVTNDTGRTYDRFRERVMFPIRDKRGRVIAFGGRILGDGVPKYLNSPETEIFH
CCCCCCEEEECCCCCHHHHHHHHCCCCEECCCCCEEEECCCHHCCCCHHHCCCCHHHHHH
KGRQLYGLYEAQVNHPNPTRLLVVEGYMDVVALAQFGIDYAVASLGTATTAEHIQLLFRA
CCHHHEEEEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
TDNVICCYDGDRAGRDAAWRALETALPYLNDGRQLRFMFLPDGEDPDTLVRKEGKDAFEQ
CCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHCCHHHHHH
RMEAAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLISQVPGEALRLYLRQQLGNKLG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
LLDDSQLDKLMPKQIDNANTYQPPQLKRTTMRILIGLLVQNPQLATLIPSLQGLEQAKLA
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHC
GLPLFIELVETCLAQPGLTTGQLLELYRDNKFSQQLETLATWNHMIVEDMVEPTFVDTLA
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SLYDSILEQRQETLIARDRTHGLNAEERKELWSLNLALARKK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]