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The map label for this gene is dkgA

Identifier: 22127375

GI number: 22127375

Start: 3883337

End: 3884170

Strand: Direct

Name: dkgA

Synonym: y3501

Alternate gene names: 22127375

Gene position: 3883337-3884170 (Clockwise)

Preceding gene: 22127373

Following gene: 22127379

Centisome position: 84.41

GC content: 48.92

Gene sequence:

>834_bases
ATGACAATGCAACCCCTTATCAAGTTGTACGATGGGCGCTTGATGCCACAACTCGGCCTCGGCGTTTGGCAGGCAAGTAT
CCAAGAAACAGAGCTTGCGGTCAGTAAGGCGCTGGAAGTCGGTTACCGCTCAATTGATACCGCCGCTATTTATAAAAATG
AAGAAGGTGTGGGCAAGGCGCTGAAAGCGGCGGCTGTTGCCCGTGATGAACTGTTTATCACCACAAAACTGTGGAACGAT
GATCAACATAATCCTCAGCAAGCCCTGGAAACCAGCCTGCAAAAGCTCCAACTGGATTATGTAGATCTCTACCTGATCCA
TTGGCCAGACCCGAAACAAGACCATTATGTCAGTGCCTGGCGTGAACTGGTCACACTAAAAGAGCAAGGTTTGATCCGCA
GTATCGGCGTGTGTAACTTCCATATTCCTCATCTGCAACGGTTGATTGATGAGACGGGTATCGCCCCTACCGTCAATCAG
ATTGAACTGCATCCGTTGCTGCAACAACGTCAGCTCCATGCCTGGAATGCCACCCATCATATTGCAACCGAGTCTTGGAG
CCCGTTAGCACAAGGTGGCAAAGGCGTTTTTGATCAAGAAATTATCCGCAAGCTCGCTCAGCAATATAATAAAACGCCTG
CACAAATCGTGATCCGTTGGCATCTGGACAGTGGGCTGATTGTTATCCCCAAATCTGTCACACCAGCACGTATCAGAGAA
AACTTTGAGGTTTTTGATTTTAAGCTACAAAAAGAGGAGTTGCTGGCAATTACCAAACTGGATTGCGGTAAACGGCTGGG
GCCAGACCCGGAAGTTTTCGGATCTGACCGCTGA

Upstream 100 bases:

>100_bases
ACTTAATAATTAGCAAGGTCATAAATAAAATGATAACGACTAAACTTACGTTATCAGCAACGGGCTAATACAGCCCAATC
AGTAAGCCAAGGAGATCAAC

Downstream 100 bases:

>100_bases
TTTCAGGCCAGCAATAACCCCTTAATGCTGCCTGTTTTTCCCTCTGGATCCGGCAGATGGCTTATTGACCGCAGCTTTAT
TTACGCCCGTTTTACCCGCT

Product: 2,5-diketo-D-gluconate reductase A

Products: NA

Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTMQPLIKLYDGRLMPQLGLGVWQASIQETELAVSKALEVGYRSIDTAAIYKNEEGVGKALKAAAVARDELFITTKLWND
DQHNPQQALETSLQKLQLDYVDLYLIHWPDPKQDHYVSAWRELVTLKEQGLIRSIGVCNFHIPHLQRLIDETGIAPTVNQ
IELHPLLQQRQLHAWNATHHIATESWSPLAQGGKGVFDQEIIRKLAQQYNKTPAQIVIRWHLDSGLIVIPKSVTPARIRE
NFEVFDFKLQKEELLAITKLDCGKRLGPDPEVFGSDR

Sequences:

>Translated_277_residues
MTMQPLIKLYDGRLMPQLGLGVWQASIQETELAVSKALEVGYRSIDTAAIYKNEEGVGKALKAAAVARDELFITTKLWND
DQHNPQQALETSLQKLQLDYVDLYLIHWPDPKQDHYVSAWRELVTLKEQGLIRSIGVCNFHIPHLQRLIDETGIAPTVNQ
IELHPLLQQRQLHAWNATHHIATESWSPLAQGGKGVFDQEIIRKLAQQYNKTPAQIVIRWHLDSGLIVIPKSVTPARIRE
NFEVFDFKLQKEELLAITKLDCGKRLGPDPEVFGSDR
>Mature_276_residues
TMQPLIKLYDGRLMPQLGLGVWQASIQETELAVSKALEVGYRSIDTAAIYKNEEGVGKALKAAAVARDELFITTKLWNDD
QHNPQQALETSLQKLQLDYVDLYLIHWPDPKQDHYVSAWRELVTLKEQGLIRSIGVCNFHIPHLQRLIDETGIAPTVNQI
ELHPLLQQRQLHAWNATHHIATESWSPLAQGGKGVFDQEIIRKLAQQYNKTPAQIVIRWHLDSGLIVIPKSVTPARIREN
FEVFDFKLQKEELLAITKLDCGKRLGPDPEVFGSDR

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG)

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family

Homologues:

Organism=Homo sapiens, GI24497577, Length=288, Percent_Identity=36.4583333333333, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI5174391, Length=288, Percent_Identity=36.4583333333333, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=35.8361774744027, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=33.6633663366337, Blast_Score=177, Evalue=6e-45,
Organism=Homo sapiens, GI310109922, Length=276, Percent_Identity=36.231884057971, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI310109920, Length=294, Percent_Identity=35.0340136054422, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI5453543, Length=294, Percent_Identity=35.0340136054422, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI45446745, Length=294, Percent_Identity=35.0340136054422, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI4503285, Length=294, Percent_Identity=35.0340136054422, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=34.5637583892617, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI291291012, Length=263, Percent_Identity=34.9809885931559, Blast_Score=163, Evalue=1e-40,
Organism=Homo sapiens, GI5174695, Length=300, Percent_Identity=34, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI24497585, Length=301, Percent_Identity=33.2225913621262, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4502049, Length=289, Percent_Identity=35.6401384083045, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI300116273, Length=278, Percent_Identity=33.8129496402878, Blast_Score=148, Evalue=4e-36,
Organism=Homo sapiens, GI300116271, Length=280, Percent_Identity=32.5, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI310109926, Length=127, Percent_Identity=43.3070866141732, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI207028673, Length=127, Percent_Identity=43.3070866141732, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI310109924, Length=146, Percent_Identity=32.8767123287671, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI87082198, Length=275, Percent_Identity=73.4545454545455, Blast_Score=426, Evalue=1e-120,
Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=41.796875, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=31.1594202898551, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17537075, Length=298, Percent_Identity=37.9194630872483, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=37.2013651877133, Blast_Score=186, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17550248, Length=293, Percent_Identity=37.542662116041, Blast_Score=180, Evalue=9e-46,
Organism=Caenorhabditis elegans, GI71998625, Length=273, Percent_Identity=35.1648351648352, Blast_Score=168, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17562292, Length=280, Percent_Identity=34.6428571428571, Blast_Score=163, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17561298, Length=273, Percent_Identity=32.6007326007326, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=34.1137123745819, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17566692, Length=283, Percent_Identity=32.8621908127208, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=31.8681318681319, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17561300, Length=272, Percent_Identity=31.9852941176471, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17552492, Length=275, Percent_Identity=30.5454545454545, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17537079, Length=304, Percent_Identity=27.6315789473684, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6324694, Length=283, Percent_Identity=38.5159010600707, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6320576, Length=275, Percent_Identity=37.4545454545455, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319625, Length=289, Percent_Identity=35.6401384083045, Blast_Score=159, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321896, Length=316, Percent_Identity=32.5949367088608, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=30.3846153846154, Blast_Score=137, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320079, Length=284, Percent_Identity=31.6901408450704, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24644950, Length=307, Percent_Identity=37.4592833876222, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI221378297, Length=327, Percent_Identity=35.1681957186544, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI20129731, Length=295, Percent_Identity=37.2881355932203, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24663317, Length=300, Percent_Identity=35.6666666666667, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24662789, Length=306, Percent_Identity=35.2941176470588, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI45553081, Length=297, Percent_Identity=36.7003367003367, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=37.5862068965517, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21356425, Length=297, Percent_Identity=35.6902356902357, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24662781, Length=297, Percent_Identity=38.7205387205387, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24662785, Length=297, Percent_Identity=38.3838383838384, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=35.6589147286822, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DKGA_YERPE (Q8ZI40)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AF0083
- RefSeq:   NP_670798.1
- RefSeq:   NP_994292.1
- RefSeq:   YP_002345742.1
- ProteinModelPortal:   Q8ZI40
- SMR:   Q8ZI40
- GeneID:   1148448
- GeneID:   1173519
- GeneID:   2766792
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO0676
- KEGG:   ypk:y3501
- KEGG:   ypm:YP_2991
- HOGENOM:   HBG605727
- OMA:   WNGDLGY
- ProtClustDB:   PRK11565
- BioCyc:   YPES187410:Y3501-MONOMER
- BioCyc:   YPES214092:YPO0676-MONOMER
- BRENDA:   1.1.1.274
- GO:   GO:0005737
- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210
- Gene3D:   G3DSA:3.20.20.100
- PANTHER:   PTHR11732
- PIRSF:   PIRSF000097
- PRINTS:   PR00069

Pfam domain/function: PF00248 Aldo_ket_red; SSF51430 Aldo/ket_red

EC number: =1.1.1.274

Molecular weight: Translated: 31521; Mature: 31389

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1; PS00062 ALDOKETO_REDUCTASE_2; PS00063 ALDOKETO_REDUCTASE_3

Important sites: ACT_SITE 51-51 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMQPLIKLYDGRLMPQLGLGVWQASIQETELAVSKALEVGYRSIDTAAIYKNEEGVGKA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHHHH
LKAAAVARDELFITTKLWNDDQHNPQQALETSLQKLQLDYVDLYLIHWPDPKQDHYVSAW
HHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHEECEEEEEEEECCCCCCCHHHHHH
RELVTLKEQGLIRSIGVCNFHIPHLQRLIDETGIAPTVNQIELHPLLQQRQLHAWNATHH
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCHHE
IATESWSPLAQGGKGVFDQEIIRKLAQQYNKTPAQIVIRWHLDSGLIVIPKSVTPARIRE
ECCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCHHHHHC
NFEVFDFKLQKEELLAITKLDCGKRLGPDPEVFGSDR
CCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMQPLIKLYDGRLMPQLGLGVWQASIQETELAVSKALEVGYRSIDTAAIYKNEEGVGKA
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHHHH
LKAAAVARDELFITTKLWNDDQHNPQQALETSLQKLQLDYVDLYLIHWPDPKQDHYVSAW
HHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHEECEEEEEEEECCCCCCCHHHHHH
RELVTLKEQGLIRSIGVCNFHIPHLQRLIDETGIAPTVNQIELHPLLQQRQLHAWNATHH
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHCCCHHE
IATESWSPLAQGGKGVFDQEIIRKLAQQYNKTPAQIVIRWHLDSGLIVIPKSVTPARIRE
ECCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEECCCCHHHHHC
NFEVFDFKLQKEELLAITKLDCGKRLGPDPEVFGSDR
CCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430