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The map label for this gene is 22127295

Identifier: 22127295

GI number: 22127295

Start: 3792240

End: 3793007

Strand: Direct

Name: 22127295

Synonym: y3420

Alternate gene names: NA

Gene position: 3792240-3793007 (Clockwise)

Preceding gene: 22127294

Following gene: 22127296

Centisome position: 82.43

GC content: 48.83

Gene sequence:

>768_bases
GTGATGATTGAACGCCTTTTTCGATTGATAAAACCGGGAACGCGTCAGCAAATGGTGTGTTTTCACCATGCAGGTGGTTC
AGCGGAGTATTTTTTGCCGTGGAAAACGGATCTGCCACCGGACACCGCCTTGTATGCCATTCAACTTCCTGGGCATGGCG
GGCGGATTAACGAATCGCTATTGCACAATTTGCATCAAATGGCTGATGTCATTGCTCAGAGTTATGTTTACCTGCCTGTG
CGTGACACGTTTTTCTTTGGCCACAGCATGGGGGCCGCTTTGGCTTTTGAGGTGGCATTGCGCTTACAAAAGGCGGGGCG
AGGCATTACCACGCTGTACGTCTCCGGGCGATTACCTCCGCATAAAAATACCAATACGGATTTTTACCGCCAGAGTGATG
TGAGTATCTTGAACAAAATCACCCAATTGGGTGGCAGCACATTGGACTACCAACAGCCAGAACTGAAAGACCTGCTATTA
CCTATTTTTAGGGCGGATTTCACCGCGATAGAGACCTACCAGCGCCAATGTACCGAACTGTTGAATTGTTCGGTTATGGC
CTGTGTGGGAGAGCAGGATACCGAAGTGTCGGTGAGGCAAATGAGTGAGTGGTGTCAGGTGACGAAGGGGGCTTTTCGGC
TACAGGTGTTCCCCGGTGGGCATTTTTATCTTAATCATGCCCAAAAAAGATTATTGGCATTCATCAACCAGTCTGTTGCC
GCGCAGCAATCAAGCAAGGAATGCAAGGAGTATCACGATGAAATATAA

Upstream 100 bases:

>100_bases
CTTATGGAAAAAGTCGGCAATCCAGAAAGTGTGCATTTTGTCGAACCGATGATGGCGTCACCTAAGGTGGCGACATTTTT
ATCTCAATGGCAGGGAGAGC

Downstream 100 bases:

>100_bases
CGCAAATATTACCAGTTTATTTGGGTTGTCCAGTTTATTTGGATTGTTCAGTCTATTTGGATTGTATTGCGCGCAAGGTG
TCCCCCTCGGGTTGGCTATC

Product: thioesterase

Products: NA

Alternate protein names: ORF-1; Protein X [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MMIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQMADVIAQSYVYLPV
RDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPHKNTNTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLL
PIFRADFTAIETYQRQCTELLNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVA
AQQSSKECKEYHDEI

Sequences:

>Translated_255_residues
MMIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQMADVIAQSYVYLPV
RDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPHKNTNTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLL
PIFRADFTAIETYQRQCTELLNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVA
AQQSSKECKEYHDEI
>Mature_255_residues
MMIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESLLHNLHQMADVIAQSYVYLPV
RDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPPHKNTNTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLL
PIFRADFTAIETYQRQCTELLNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVA
AQQSSKECKEYHDEI

Specific function: Probable thioesterase involved in the biosynthesis of candicidin. Could release the macrolide ring from the polyketide synthase [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=244, Percent_Identity=27.4590163934426, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8922871, Length=202, Percent_Identity=28.2178217821782, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR010693
- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF06902 DUF1271; PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28919; Mature: 28919

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESL
CHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCHHH
LHNLHQMADVIAQSYVYLPVRDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPP
HHHHHHHHHHHHHCEEEEEEHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
HKNTNTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLPIFRADFTAIETYQRQCTEL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVA
HCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHH
AQQSSKECKEYHDEI
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MMIERLFRLIKPGTRQQMVCFHHAGGSAEYFLPWKTDLPPDTALYAIQLPGHGGRINESL
CHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCHHH
LHNLHQMADVIAQSYVYLPVRDTFFFGHSMGAALAFEVALRLQKAGRGITTLYVSGRLPP
HHHHHHHHHHHHHCEEEEEEHHHEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
HKNTNTDFYRQSDVSILNKITQLGGSTLDYQQPELKDLLLPIFRADFTAIETYQRQCTEL
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNCSVMACVGEQDTEVSVRQMSEWCQVTKGAFRLQVFPGGHFYLNHAQKRLLAFINQSVA
HCCCEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHH
AQQSSKECKEYHDEI
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8472954 [H]