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The map label for this gene is pepT [C]

Identifier: 22127279

GI number: 22127279

Start: 3766820

End: 3768052

Strand: Direct

Name: pepT [C]

Synonym: y3403

Alternate gene names: 22127279

Gene position: 3766820-3768052 (Clockwise)

Preceding gene: 22127278

Following gene: 22127280

Centisome position: 81.87

GC content: 49.8

Gene sequence:

>1233_bases
ATGACTATCGCACTCTCAGAACAACTGACAAACCGCTTTTTCCGTTATCTGGCGGTTAGCAGCCAGAGCGATGCCGCCTC
TCCAACACTGCCGAGTACCGAAGGGCAGCACAAAATGGCGCAGATGTTAGCTGAAGAACTGCGTCAGTTGGGGTTGGAAG
ATATCCTGATTGATGAACATGCCACGGTGACAGCCCGTAAACCCGGCAATCAGCCTACCGCACCACGCATTGGTTTTATT
ACACATATTGATACGGTCGACGTTGGCTTATCGCCCGATATTCATCCGCAACGCTTACGCTTTACCGGTAGCGATCTTTG
CCTAAACGCAGAGCAGGGCATCTATCTACGTACCGCAGAACATCCGGAAATCCTGCGTTATCAGGGCGAAGAGATTATTT
TCAGCGATGGCACCAGTGTACTAGGCGCAGATAATAAAGCGGCAGTGACCGTAGTCATGACGTTGTTGGAAAACCTGACG
GCTGACGATTGCCATGGCGATATCGTGGTGGCGTTTGTTCCTGACGAAGAGATTGGTCTGCGCGGTGCTAAAGCGCTGGA
TCTGGCGCGTTTTGATGTTGATTTCGCTTACACTATTGACTGCTGCGAGCTGGGCGAAGTGGTGTATGAAAACTTTAACG
CCGCCTCGGCAGAGATCGATATCATCGGGGTGACGGCTCACCCGATGTCAGCTAAAAATGTGCTGATCAACCCTATCCGT
GTCGCCTATGACATTATCAGTGAGTTCTCGCCACAAGAGACGCCAGAACACACTGAAGGGCGGGAAGGCTATGTCTGGTT
TACCGATATGACTGCCAACCCTAACAGCGCAAAACTGAAAATAGCAATCCGTGATTTTGATAATGTGTCATTCGCCGCGC
GTAAAGCCTATATCGGTGAAGTGGTCGCGAAAGTTTCAGCACAATATCCACGAGCGAAGATCAGCTATTCAGTCACCGAT
GTGTACAGCAATATCAGTAACTCGATTGGTGAAGATAAGCGGGCTATCGACCTGATCTTCTCTTCTATGGCGGAACTGAA
TATCGAACCTAAAGTGATCCCTATGCGCGGCGGTACCGATGGTGCGGCGTTATCAACCCAAGGGTTGCTGACCCCAAATT
ATTTTACCGGGGCGCATAATTTCCACTCACCGTTCGAATTTTTACCGATCAGCTCCTTTGTGAAATCATATCAATTAACC
CGGACGATCTGTTTGTCTGCGGCCAAAGCGTAA

Upstream 100 bases:

>100_bases
ATACGTATCCAGCAACTCAATGACCCCCTTCGGCCTTGACGCTGTAGGGGTGTTAGCTGCGCGCACTCAGTGGTTTTAGC
TATAAATAAAGGAATAAAAA

Downstream 100 bases:

>100_bases
AAGCTGGAAAGGTATTGATATTAAATGCCTTATCTCCATGTAGGGTACTGGCGTGTTAGCTTGCCAGTACCCTATTAATG
TTGTGACTTCTGCTGTACGC

Product: peptidase T

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTIALSEQLTNRFFRYLAVSSQSDAASPTLPSTEGQHKMAQMLAEELRQLGLEDILIDEHATVTARKPGNQPTAPRIGFI
THIDTVDVGLSPDIHPQRLRFTGSDLCLNAEQGIYLRTAEHPEILRYQGEEIIFSDGTSVLGADNKAAVTVVMTLLENLT
ADDCHGDIVVAFVPDEEIGLRGAKALDLARFDVDFAYTIDCCELGEVVYENFNAASAEIDIIGVTAHPMSAKNVLINPIR
VAYDIISEFSPQETPEHTEGREGYVWFTDMTANPNSAKLKIAIRDFDNVSFAARKAYIGEVVAKVSAQYPRAKISYSVTD
VYSNISNSIGEDKRAIDLIFSSMAELNIEPKVIPMRGGTDGAALSTQGLLTPNYFTGAHNFHSPFEFLPISSFVKSYQLT
RTICLSAAKA

Sequences:

>Translated_410_residues
MTIALSEQLTNRFFRYLAVSSQSDAASPTLPSTEGQHKMAQMLAEELRQLGLEDILIDEHATVTARKPGNQPTAPRIGFI
THIDTVDVGLSPDIHPQRLRFTGSDLCLNAEQGIYLRTAEHPEILRYQGEEIIFSDGTSVLGADNKAAVTVVMTLLENLT
ADDCHGDIVVAFVPDEEIGLRGAKALDLARFDVDFAYTIDCCELGEVVYENFNAASAEIDIIGVTAHPMSAKNVLINPIR
VAYDIISEFSPQETPEHTEGREGYVWFTDMTANPNSAKLKIAIRDFDNVSFAARKAYIGEVVAKVSAQYPRAKISYSVTD
VYSNISNSIGEDKRAIDLIFSSMAELNIEPKVIPMRGGTDGAALSTQGLLTPNYFTGAHNFHSPFEFLPISSFVKSYQLT
RTICLSAAKA
>Mature_409_residues
TIALSEQLTNRFFRYLAVSSQSDAASPTLPSTEGQHKMAQMLAEELRQLGLEDILIDEHATVTARKPGNQPTAPRIGFIT
HIDTVDVGLSPDIHPQRLRFTGSDLCLNAEQGIYLRTAEHPEILRYQGEEIIFSDGTSVLGADNKAAVTVVMTLLENLTA
DDCHGDIVVAFVPDEEIGLRGAKALDLARFDVDFAYTIDCCELGEVVYENFNAASAEIDIIGVTAHPMSAKNVLINPIRV
AYDIISEFSPQETPEHTEGREGYVWFTDMTANPNSAKLKIAIRDFDNVSFAARKAYIGEVVAKVSAQYPRAKISYSVTDV
YSNISNSIGEDKRAIDLIFSSMAELNIEPKVIPMRGGTDGAALSTQGLLTPNYFTGAHNFHSPFEFLPISSFVKSYQLTR
TICLSAAKA

Specific function: Releases The N-Terminal Amino Acid From Tripeptides. Has A Preference For Tripeptides Containing A N-Terminal Methionine Or Leucine (By Similarity). [C]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family

Homologues:

Organism=Escherichia coli, GI1787372, Length=408, Percent_Identity=38.2352941176471, Blast_Score=279, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1009_YERPE (Q8ZH96)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0123
- RefSeq:   NP_670702.1
- RefSeq:   NP_994705.1
- RefSeq:   YP_002346054.1
- ProteinModelPortal:   Q8ZH96
- SMR:   Q8ZH96
- IntAct:   Q8ZH96
- MEROPS:   M20.003
- GeneID:   1148350
- GeneID:   1173847
- GeneID:   2765834
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1009
- KEGG:   ypk:y3403
- KEGG:   ypm:YP_3421
- HOGENOM:   HBG288638
- OMA:   CFVPDEE
- ProtClustDB:   PRK13381
- BioCyc:   YPES187410:Y3403-MONOMER
- BioCyc:   YPES214092:YPO1009-MONOMER
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00550
- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161
- PIRSF:   PIRSF037215
- TIGRFAMs:   TIGR01882

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: 3.4.11.4 [C]

Molecular weight: Translated: 44891; Mature: 44760

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 84-84 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIALSEQLTNRFFRYLAVSSQSDAASPTLPSTEGQHKMAQMLAEELRQLGLEDILIDEH
CEEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCC
ATVTARKPGNQPTAPRIGFITHIDTVDVGLSPDIHPQRLRFTGSDLCLNAEQGIYLRTAE
CEEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHEEECCCCEEEECCCCEEEEECC
HPEILRYQGEEIIFSDGTSVLGADNKAAVTVVMTLLENLTADDCHGDIVVAFVPDEEIGL
CCHHHHCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
RGAKALDLARFDVDFAYTIDCCELGEVVYENFNAASAEIDIIGVTAHPMSAKNVLINPIR
CCCCEEEEEEECCEEEEEECHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEECHHH
VAYDIISEFSPQETPEHTEGREGYVWFTDMTANPNSAKLKIAIRDFDNVSFAARKAYIGE
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHH
VVAKVSAQYPRAKISYSVTDVYSNISNSIGEDKRAIDLIFSSMAELNIEPKVIPMRGGTD
HHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
GAALSTQGLLTPNYFTGAHNFHSPFEFLPISSFVKSYQLTRTICLSAAKA
CCCEECCCCCCCCEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIALSEQLTNRFFRYLAVSSQSDAASPTLPSTEGQHKMAQMLAEELRQLGLEDILIDEH
EEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCC
ATVTARKPGNQPTAPRIGFITHIDTVDVGLSPDIHPQRLRFTGSDLCLNAEQGIYLRTAE
CEEEEECCCCCCCCCCEEEEEEEEEEECCCCCCCCHHHEEECCCCEEEECCCCEEEEECC
HPEILRYQGEEIIFSDGTSVLGADNKAAVTVVMTLLENLTADDCHGDIVVAFVPDEEIGL
CCHHHHCCCCEEEEECCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
RGAKALDLARFDVDFAYTIDCCELGEVVYENFNAASAEIDIIGVTAHPMSAKNVLINPIR
CCCCEEEEEEECCEEEEEECHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCEEECHHH
VAYDIISEFSPQETPEHTEGREGYVWFTDMTANPNSAKLKIAIRDFDNVSFAARKAYIGE
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHH
VVAKVSAQYPRAKISYSVTDVYSNISNSIGEDKRAIDLIFSSMAELNIEPKVIPMRGGTD
HHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCC
GAALSTQGLLTPNYFTGAHNFHSPFEFLPISSFVKSYQLTRTICLSAAKA
CCCEECCCCCCCCEECCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430