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The map label for this gene is rlmH

Identifier: 221232936

GI number: 221232936

Start: 2218629

End: 2219108

Strand: Reverse

Name: rlmH

Synonym: SPN23F_22710

Alternate gene names: 221232936

Gene position: 2219108-2218629 (Counterclockwise)

Preceding gene: NA

Following gene: 221232935

Centisome position: 99.9

GC content: 33.33

Gene sequence:

>480_bases
ATGAAAATTAAAGTTGTAACAGTTGGGAAACTGAAAGAAAAGTATTTAAAAGATGGTATCGCAGAGTATTCAAAACGAAT
TTCTAGATTTGCTAAGTTTGAAATGATTGAGTTATCAGATGAAAAAACACCAGATAAGGCCAGTGAATCAGAAAATCAAA
AGATTTTAGAAATAGAAGGTCAGAGAATTTTATCAAAAATTGCTGACCGTGATTTCGTTATTGTGTTAGCCATTGAAGGG
AAAACTTTCTTCTCAGAAGAATTTAGTAAGCAGTTAGAAGAAACTTCTATAAAAGGATTTTCTACTCTTACTTTTATTAT
TGGGGGAAGTTTAGGATTGTCATCATCTGTAAAAAATAGAGCCAATCTTTCTGTCAGTTTTGGTCGCCTAACCTTGCCTC
ATCAGTTAATGAGACTAGTTCTTGTTGAACAAATCTATCGCGCTTTTACGATTCAGCAGGGATTCCCCTACCATAAATAG

Upstream 100 bases:

>100_bases
TGTGGATAACTCTTTTGAAACTGTGATTTTCTTAATTGAAATCTATTTTTTATTTTGTGAATAAGATGTGAAAAAATAGA
GAATATGTTAGAATAGAGTC

Downstream 100 bases:

>100_bases
AAAATTGACTTTTAATTGAATTTTTGGTAGAATAATTGTGTTAGGTCTCATAGCTCAGCTGGATAGAGCATTCGCCTTCT
AAGCGAACGGTCGCAGGTTC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPDKASESENQKILEIEGQRILSKIADRDFVIVLAIEG
KTFFSEEFSKQLEETSIKGFSTLTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK

Sequences:

>Translated_159_residues
MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPDKASESENQKILEIEGQRILSKIADRDFVIVLAIEG
KTFFSEEFSKQLEETSIKGFSTLTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK
>Mature_159_residues
MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPDKASESENQKILEIEGQRILSKIADRDFVIVLAIEG
KTFFSEEFSKQLEETSIKGFSTLTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=159, Percent_Identity=32.7044025157233, Blast_Score=103, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_STRP2 (Q04HU2)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817446.1
- ProteinModelPortal:   Q04HU2
- SMR:   Q04HU2
- STRING:   Q04HU2
- EnsemblBacteria:   EBSTRT00000020604
- GeneID:   4442870
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_2067
- eggNOG:   COG1576
- GeneTree:   EBGT00050000028023
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 18099; Mature: 18099

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPDKASESENQKILEIEG
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEHH
QRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFSTLTFIIGGSLGLSSSVKNR
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCHHCCCC
ANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTPDKASESENQKILEIEG
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCEEEEEHH
QRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFSTLTFIIGGSLGLSSSVKNR
HHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCHHCCCC
ANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA