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The map label for this gene is trsA

Identifier: 221232927

GI number: 221232927

Start: 2208064

End: 2209089

Strand: Reverse

Name: trsA

Synonym: SPN23F_22620

Alternate gene names: 221232927

Gene position: 2209089-2208064 (Counterclockwise)

Preceding gene: 221232930

Following gene: 221232926

Centisome position: 99.45

GC content: 38.79

Gene sequence:

>1026_bases
ATGACTAAACCCATTATTTTAACAGGAGACCGTCCAACAGGAAAATTGCATATTGGACATTATGTTGGAAGTCTCAAAAA
TCGAGTATTATTACAGGAAGAGGATAAGTATGATATGTTTGTGTTCTTGGCTGACCAACAAGCCTTGACAGATCATGCCA
AAGATCCTCAAACCATTGTAGAGTCTATCGGAAATGTGGCTTTGGATTATCTTGCAGTTGGATTGGATCCAAATAAGTCA
ACTATTTTTATTCAAAGCCAGATTCCAGAGTTGGCTGAGTTGTCTATGTATTATATGAATCTAGTTTCGTTAGCACGTTT
GGAGCGAAATCCAACAGTCAAGACAGAGATTTCTCAGAAAGGATTTGGAGAAAGCATTCCGACAGGATTCTTGGTCTATC
CAATCGCTCAAGCAGCTGATATCACAGCTTTCAAGGCTAATTATGTTCCTGTTGGGACAGATCAGAAACCAATGATTGAG
CAAACTCGTGAAATTGTTCGTTCTTTTAACAATGCATATAACTGTGATGTCTTGGTAGAGCCGGAAGGTATTTATCCAGA
AAATGAGAGAGCAGGGCGTTTGCCTGGTTTAGATGGAAATGCTAAAATGTCTAAATCACTAAATAATGGTATTTATTTAG
CTGATGATGCGGATACTTTGCGTAAAAAAGTAATGAGTATGTATACAGATCCAGATCATATCCGCGTTGAGGATCCAGGT
AAGATTGAGGGAAATATGGTTTTCCATTATCTAGATGTTTTTGGTCGTCCAGAAGATGCTCAAGAAATTGCTGATATGAA
AGAACGTTATCAACGAGGTGGTCTTGGTGATGTGAAGACCAAGCGTTATCTACTTGAAATATTAGAACGTGAACTGGGTC
CTATTCGTGAGCGCCGTATTGAATTTGCTAAGGATATGGGAGAAGTTTATAATATGATTCAAAAAGGTAGTGAAAGAGCG
CGTGAAGTTGCGGGTCAAACCCTATCTGAGGTAAAAGGGGCAATGGGACTTCATTACTTTAACTAA

Upstream 100 bases:

>100_bases
CACAAATCTGTAAAAAGCAAGGCTGATTTATTTTGATAAATTACGGTTATTTCATTAAAAAAATGCTATAATTGAAAGGA
CTATATCGAAGGAGAACAAA

Downstream 100 bases:

>100_bases
GTTTATTTTACAAGAAACTATCTTTTCTATCTCGAAGAAAAGATAGTTTTTTATTTACCCCTGTAACATTTGACAAATTT
TTATAGAATGGTATGATAGA

Product: tryptophanyl-tRNA synthetase II

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLADQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKS
TIFIQSQIPELAELSMYYMNLVSLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDADTLRKKVMSMYTDPDHIRVEDPG
KIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLGDVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERA
REVAGQTLSEVKGAMGLHYFN

Sequences:

>Translated_341_residues
MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLADQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKS
TIFIQSQIPELAELSMYYMNLVSLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDADTLRKKVMSMYTDPDHIRVEDPG
KIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLGDVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERA
REVAGQTLSEVKGAMGLHYFN
>Mature_340_residues
TKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLADQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKST
IFIQSQIPELAELSMYYMNLVSLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIEQ
TREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDADTLRKKVMSMYTDPDHIRVEDPGK
IEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLGDVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAR
EVAGQTLSEVKGAMGLHYFN

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=344, Percent_Identity=29.9418604651163, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI41352700, Length=184, Percent_Identity=34.2391304347826, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789786, Length=340, Percent_Identity=32.0588235294118, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71982800, Length=350, Percent_Identity=27.7142857142857, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI71982793, Length=348, Percent_Identity=27.5862068965517, Blast_Score=124, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6320474, Length=358, Percent_Identity=27.9329608938547, Blast_Score=114, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24666151, Length=343, Percent_Identity=32.0699708454811, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_STRPN (P67595)

Other databases:

- EMBL:   AE005672
- PIR:   D95260
- RefSeq:   NP_346637.1
- ProteinModelPortal:   P67595
- SMR:   P67595
- EnsemblBacteria:   EBSTRT00000026010
- GeneID:   930554
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_2229
- TIGR:   SP_2229
- GeneTree:   EBGT00050000027780
- HOGENOM:   HBG293263
- OMA:   TYLDAFH
- ProtClustDB:   PRK12282
- BioCyc:   SPNE170187-1:SP_2229-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 38418; Mature: 38287

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLADQQALTDHAKDPQTIV
CCCCEEEECCCCCCEEEHHHHHHHHHCCEEEEECCCCEEEEEEECCHHHHHCCCCHHHHH
ESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLVSLARLERNPTVKTEISQK
HHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHC
GFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIEQTREIVRSFNNAYNCDVLVE
CCCCCCCCCEEEEEHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEC
PEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDADTLRKKVMSMYTDPDHIRVEDPG
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCEEECCCC
KIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLGDVKTKRYLLEILERELGPIRERRI
CCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHH
EFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGAMGLHYFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLADQQALTDHAKDPQTIV
CCCEEEECCCCCCEEEHHHHHHHHHCCEEEEECCCCEEEEEEECCHHHHHCCCCHHHHH
ESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLVSLARLERNPTVKTEISQK
HHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHC
GFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIEQTREIVRSFNNAYNCDVLVE
CCCCCCCCCEEEEEHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEC
PEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDADTLRKKVMSMYTDPDHIRVEDPG
CCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCEEECCCC
KIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLGDVKTKRYLLEILERELGPIRERRI
CCCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHH
EFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGAMGLHYFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916