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The map label for this gene is tsf [H]

Identifier: 221232912

GI number: 221232912

Start: 2193606

End: 2194646

Strand: Reverse

Name: tsf [H]

Synonym: SPN23F_22470

Alternate gene names: 221232912

Gene position: 2194646-2193606 (Counterclockwise)

Preceding gene: 221232913

Following gene: 221232909

Centisome position: 98.8

GC content: 41.11

Gene sequence:

>1041_bases
ATGGCAGAAATTACAGCTAAACTTGTAAAAGAGTTGCGTGAAAAATCTGGTGCCGGTGTTATGGACGCTAAAAAAGCGCT
TGTAGAAACAGACGGTGACATCGAAAAAGCGATTGAATTGCTTCGTGAAAAAGGTATGGCTAAGGCAGCTAAGAAAGCTG
ACCGTGTTGCTGCAGAAGGTTTGACTGGTGTTTATGTTAACGGTAATGTTGCAGCAGTTATTGAAGTAAACGCTGAAACT
GACTTCGTTGCAAAAAACGCTCAATTCGTTGAATTGGTAAATACTACAGCTAAAGTCATTGCTGAAGGAAAACCTGCTAA
CAATGAAGAAGCTCTTGCTTTGATAATGCCTTCAGGTGAAACTCTTGAAGCTGCATACGTATCTGCAACAGCAACTATCG
GAGAGAAAATCTCATTCCGTCGCTTTGCATTGATTGAAAAAACAGACGCACAACACTTTGGAGCATACCAACATAACGGT
GGACGTATCGGTGTTATTTCAGTTGTTGAAGGTGGAGACGAAGCACTTGCTAAACAATTGTCAATGCACATCGCAGCGAT
GAAACCAACAGTTCTTTCTTACAAAGAATTGGATGAGCAATTCGTTAAAGATGAGTTGGCACAATTGAATCACGTTATCG
ACCAAGACAACGAAAGCCGTGCAATGGTTAATAAACCAGCTCTTCCACACTTGAAGTATGGATCAAAAGCTCAATTAACT
GATGATGTTATTGCTCAAGCTGAAGCTGACATCAAAGCTGAATTGGCTGCAGAAGGCAAACCAGAAAAAATCTGGGACAA
AATTATTCCAGGTAAAATGGATCGCTTCATGCTTGATAACACTAAAGTTGACCAAGCTTACACACTTCTTGCACAAGTTT
ACATCATGGATGACAGCAAGACAGTTGAAGCATACCTTGAATCAGTTAACGCTTCGGTAGTTGAGTTTGCTCGCTTTGAA
GTTGGTGAAGGTATCGAGAAAGCTGCAAACGACTTCGAAGCTGAAGTTGCAGCTACAATGGCAGCAGCCTTGAATAACTA
A

Upstream 100 bases:

>100_bases
TGTAGAAGGTGACAACGCTTAATTTATACAAATAGTAATTACCTAGGAGGGCGGGGCTTAGCCCGGCTCTCCTATTTTCA
AAAAATATAGGAGAATTAAA

Downstream 100 bases:

>100_bases
TAATAGAAAGGAAGCAATTTGCTTCCTTTTTTGTATAGGGCTTATATTTTTCCTGAAATTGTTTTTCCCCCAGTCTCACT
CGATTAGAATAATTAAAACA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREKGMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAET
DFVAKNAQFVELVNTTAKVIAEGKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNESRAMVNKPALPHLKYGSKAQLT
DDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFE
VGEGIEKAANDFEAEVAATMAAALNN

Sequences:

>Translated_346_residues
MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREKGMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAET
DFVAKNAQFVELVNTTAKVIAEGKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNESRAMVNKPALPHLKYGSKAQLT
DDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFE
VGEGIEKAANDFEAEVAATMAAALNN
>Mature_345_residues
AEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREKGMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETD
FVAKNAQFVELVNTTAKVIAEGKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNGG
RIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNESRAMVNKPALPHLKYGSKAQLTD
DVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEV
GEGIEKAANDFEAEVAATMAAALNN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=232, Percent_Identity=28.8793103448276, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI291084500, Length=253, Percent_Identity=26.4822134387352, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI291084498, Length=94, Percent_Identity=42.5531914893617, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI291084502, Length=94, Percent_Identity=42.5531914893617, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786366, Length=338, Percent_Identity=39.0532544378698, Blast_Score=197, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17561440, Length=226, Percent_Identity=28.3185840707965, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921466, Length=212, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 37362; Mature: 37231

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREKGMAKAAKKADRVAAEG
CCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
LTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAEGKPANNEEALALIMPSGE
CCEEEECCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
TLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNGGRIGVISVVEGGDEALAKQL
CCHHHHHHHHHHHHCHHCHHHEEEEECCCHHHCCCEECCCCEEEEEEEECCCHHHHHHHH
SMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNESRAMVNKPALPHLKYGSKAQLT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCH
DDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSK
HHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHEEEECCCH
TVEAYLESVNASVVEFARFEVGEGIEKAANDFEAEVAATMAAALNN
HHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREKGMAKAAKKADRVAAEG
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
LTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAEGKPANNEEALALIMPSGE
CCEEEECCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
TLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNGGRIGVISVVEGGDEALAKQL
CCHHHHHHHHHHHHCHHCHHHEEEEECCCHHHCCCEECCCCEEEEEEEECCCHHHHHHHH
SMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNESRAMVNKPALPHLKYGSKAQLT
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCH
DDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRFMLDNTKVDQAYTLLAQVYIMDDSK
HHHHHHHCCCHHHHHHCCCCHHHHHHHHCCCCHHHHEECCCCHHHHHHHHHHEEEECCCH
TVEAYLESVNASVVEFARFEVGEGIEKAANDFEAEVAATMAAALNN
HHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA