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The map label for this gene is tgt [H]

Identifier: 221232773

GI number: 221232773

Start: 2017109

End: 2018251

Strand: Reverse

Name: tgt [H]

Synonym: SPN23F_20810

Alternate gene names: 221232773

Gene position: 2018251-2017109 (Counterclockwise)

Preceding gene: 221232775

Following gene: 221232772

Centisome position: 90.86

GC content: 44.71

Gene sequence:

>1143_bases
ATGTCAGATTCACCAATCAAATATCGTTTGATTAAGAAAGAAAAACACACAGGAGCTCGTCTGGGAGAAATCATCACTCC
CCACGGTACCTTTCCGACACCTATGTTTATGCCAGTTGGGACACAAGCCACTGTCAAAACTCAGTCACCTGAAGAATTGA
AGGAGATGGGTTCGGGAATTATCCTATCAAACACCTATCATCTCTGGCTTCGCCCTGGAGATGAACTCATTGCACGCGCT
GGTGGTCTCCACAAGTTCATGAATTGGGACCAGCCTATCTTGACAGATAGTGGTGGTTTTCAGGTTTATTCTTTAGCAGA
TAGCCGTAATATCACAGAAGAAGGAGTAACCTTTAAAAATCATCTAAATGGTTCTAAGATGTTCCTATCCCCAGAAAAAG
CCATCTCTATTCAGAATAATCTGGGTTCAGACATCATGATGTCCTTTGATGAATGTCCTCAGTTTTATCAACCTTATGAC
TACGTTAAGAAATCGATCGAGCGTACCAGCCGTTGGGCTGAGCGTGGTTTGAAGGCTCACCGTCGTCCACATGACCAAGG
TTTGTTTGGAATTGTGCAAGGTGCAGGATTTGAAGACCTTCGCCGCCAATCAGCTCATGATCTTGTCAGCATGGATTTCT
CAGGCTACTCTATCGGTGGTTTGGCAGTGGGAGAAACCCATGAAGAGATGAATGCGGTCTTGGACTTTACAACTCAACTG
CTACCTGAAAATAAACCTCGTTATCTGATGGGTGTGGGAGCGCCAGATAGCTTGATCGATGGGGTCATTCGTGGGGTGGA
TATGTTTGACTGTGTCTTACCGACTCGAATTGCTCGTAACGGGACTTGTATGACCAGTCAAGGACGTTTGGTTGTGAAAA
ATGCCCAGTTTGCTGAGGACTTTACGCCACTGGATCCTGAGTGTGACTGCTACACATGTAATAACTATACACGCGCTTAC
CTTCGTCACCTGCTCAAGGCTGATGAAACCTTTGGTATCCGCTTGACTAGCTACCACAATCTTTACTTCTTGCTTAACCT
GATGAAGCAAGTGCGACAAGCCATCATGGATGACAATCTCTTGGAATTCCGTGAGTATTTTGTGGAAAAATATGGCTATA
ATAAGTCAGGACGCAATTTCTAA

Upstream 100 bases:

>100_bases
AGTTCGTGACAGTTCCTACTTTTTTTGATAAAATCATACAGTGTGTCCTTGGGCACACTGTATGAACTGGGACTGTCTTT
CCCAGCTTCGGAGGTAAAAA

Downstream 100 bases:

>100_bases
AACGGAATAGATATAAGCTAAAAATCCTAAGTTTTCTCTTGGGATTTTTCTTCTTTTTTTGATAGAATAAAGTGTATAAT
GAAAGGGAGAATAAACTCGT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARA
GGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSIGGLAVGETHEEMNAVLDFTTQL
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAY
LRHLLKADETFGIRLTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF

Sequences:

>Translated_380_residues
MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARA
GGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSIGGLAVGETHEEMNAVLDFTTQL
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAY
LRHLLKADETFGIRLTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF
>Mature_379_residues
SDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAG
GLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYDY
VKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSIGGLAVGETHEEMNAVLDFTTQLL
PENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYL
RHLLKADETFGIRLTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=373, Percent_Identity=44.2359249329759, Blast_Score=335, Evalue=4e-92,
Organism=Escherichia coli, GI1786607, Length=355, Percent_Identity=50.9859154929577, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=45.4802259887006, Blast_Score=305, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25146502, Length=207, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=42.7792915531335, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI21355311, Length=229, Percent_Identity=25.764192139738, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43122; Mature: 42990

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEMGSGI
CCCCCEEEEEEECCCCCCHHHCCEECCCCCCCCCEEEECCCCEEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKN
EEECEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCHHCCCHHH
HLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAH
CCCCCEEEECHHHHEEECCCCCHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSIGGLAVGETHEEMNAVLDFTTQL
CCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCEEECCEEECCCHHHHHHHHHHHHHH
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSQGRLVVKNAQFAED
CCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHC
FTPLDPECDCYTCNNYTRAYLRHLLKADETFGIRLTSYHNLYFLLNLMKQVRQAIMDDNL
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCH
LEFREYFVEKYGYNKSGRNF
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEMGSGI
CCCCEEEEEEECCCCCCHHHCCEECCCCCCCCCEEEECCCCEEEECCCHHHHHHHCCCE
ILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQVYSLADSRNITEEGVTFKN
EEECEEEEEECCCHHHHHHHCCHHHHHCCCCCEEECCCCEEEEEECCCCCCCHHCCCHHH
HLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAH
CCCCCEEEECHHHHEEECCCCCHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSIGGLAVGETHEEMNAVLDFTTQL
CCCCCCCCEEEECCCCHHHHHHHHHHHEEEECCCCEEECCEEECCCHHHHHHHHHHHHHH
LPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSQGRLVVKNAQFAED
CCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHC
FTPLDPECDCYTCNNYTRAYLRHLLKADETFGIRLTSYHNLYFLLNLMKQVRQAIMDDNL
CCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCH
LEFREYFVEKYGYNKSGRNF
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA