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The map label for this gene is tkt [H]

Identifier: 221232748

GI number: 221232748

Start: 1992532

End: 1994508

Strand: Reverse

Name: tkt [H]

Synonym: SPN23F_20510

Alternate gene names: 221232748

Gene position: 1994508-1992532 (Counterclockwise)

Preceding gene: 221232749

Following gene: 221232747

Centisome position: 89.79

GC content: 44.26

Gene sequence:

>1977_bases
ATGTCAAATCTATCTGTTAATGCAATTCGTTTTCTAGGTATTGACGCCATTAATAAAGCCAACTCAGGTCATCCAGGTGT
GGTTATGGGAGCGGCTCCGATGGCTTACAGCCTCTTTACAAAACAACTTCATATCAATCCAGCTCAACCAAATTGGATTA
ACCGCGACCGCTTTATTCTTTCAGCAGGTCATGGTTCAATGCTCCTTTATGCTCTTCTTCACCTTTCTGGTTTTGAAGAT
GTCAGCATGGATGAGATTAAGAGTTTCCGTCAATGGGGTTCAAAAACACCAGGTCACCCAGAATTTGGTCATACGGCAGG
GATTGATGCTACGACAGGTCCTCTAGGGCAAGGGATTTCAACTGCTACTGGTTTTGCCCAAGCAGAACGTTTCTTGGCAG
CCAAATATAACCGTGAAGGCTACAATATCTTTGACCACTATACTTACGTTATCTGTGGAGACGGAGACTTGATGGAAGGT
GTCTCAAGCGAGGCAGCTTCATACGCAGGTTTGCAAAAACTTGATAAGTTGGTTGTTCTTTATGATTCAAATGATATCAA
CTTGGATGGTGAGACAAAGGATTCCTTTACAGAAAGTGTTCGTGACCGTTACAATGCCTACGGTTGGCATACTGCCTTGG
TTGAAAATGGAACAGACTTGGAAGCCATCCATGCTGCTATCGAAACAGCAAAAGCTTCAGGCAAGCCATCTTTAATTGAA
GTGAAGACGGTTATTGGATATGGTTCTCCAAACAAACAAGGAACTAATGCTGTACACGGCGCCCCTCTTGGAGCAGATGA
AACCGCATCAACTCGCCAAGCCCTCGGTTGGGACTACGAACCATTTGAAATTCCAGAACAAGTATATGCTGATTTCAAAG
AACATGTTGCAGACCGTGGCGCATCAGCTTATCAAGCTTGGACTAAATTAGTTGCAGATTATAAAGAAGCTCATCCAGAA
CTGGCTGCAGAAGTAGAAGCCATCATCGACGGACGTGATCCAGTCGAAGTGACTCCAGCAGACTTCCCAGCTTTAGAAAA
TGGTTTTTCTCAAGCAACTCGTAATTCAAGTCAAGATGCCTTGAATGTTGTGGCAGCTAAATTACCAACCTTCCTAGGTG
GATCAGCTGACCTTGCTCACTCAAACATGACTTATATCAAGACGGATGGACTTCAAGACGATACCAATCGCTTGAATCGT
AACATTCAGTTTGGTGTTCGTGAATTTGCAATGGGAACAATCTTGAACGGGATGGCCCTTCATGGTGGACTTCGTGTATA
CGGTGGAACTTTCTTCGTCTTCTCTGACTATGTGAAGGCAGCTGTCCGCTTGTCAGCCTTACAAGGACTTCCTGTGACTT
ATGTCTTTACCCATGATTCAATCGCAGTTGGGGAAGATGGTCCGACTCACGAACCAGTTGAGCATTTAGCAGGTCTTCGT
GCTATGCCAAATCTAAATGTTTTCCGTCCAGCAGATGCGCGTGAAACGCAAGCAGCTTGGTACCTTGCAGTGACAAGTGA
GAAAACACCAACTGCCCTTGTCTTGACACGTCAAAACTTGACTGTTGAAGATGGAACAGACTTCGACAAGGTTGCTAAAG
GTGCTTATGTTGTATATGAAAATGCAGCCGACTTTGATACCATCTTGATTGCGACAGGTTCAGAGGTTAATCTTGCTGTC
TCAGCTGCTAAAGAATTGGCTAGTCAAGGCGAAAAAATCCGCGTAGTCAGCATGCCATCTACAGATGTCTTTGATAAACA
AGATGCAGCTTACAAGGAAGAGATTCTTCCAAATGCAGTCCGCCGTCGTGTTGCAGTCGAAATGGGTGCAAGTCAAAACT
GGTACAAATATGTTGGTCTCGATGGTGCCGTTCTAGGTATTGATACCTTCGGAGCCTCTGCCCCAGCACCAAAAGTATTG
GCAGAATATGGCTTTACTGTAGAAAATCTTGTAAAAATTGTTCGAAACTTGAAATAA

Upstream 100 bases:

>100_bases
TTTGGAGGTTATCCGTATGTTGCATGATACGGATAATTTTCATATAATAGTAAAATAGAATGTGTGATTCAATAATCACC
TCAAATAGAAAGGAAATTCT

Downstream 100 bases:

>100_bases
TCCTAAAAATCAGGGCGTAAGCTCTGGTTTTTCTTACCAGAAAAGTAAGGTACAATCTTGTAAAAGTAGCTGAAATTTGA
TATAGTAGTCCTATGTAAAA

Product: transketolase

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 658; Mature: 657

Protein sequence:

>658_residues
MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLHINPAQPNWINRDRFILSAGHGSMLLYALLHLSGFED
VSMDEIKSFRQWGSKTPGHPEFGHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGTDLEAIHAAIETAKASGKPSLIE
VKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALGWDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPE
LAAEVEAIIDGRDPVEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQDDTNRLNR
NIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGLPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
AMPNLNVFRPADARETQAAWYLAVTSEKTPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAV
SAAKELASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAVLGIDTFGASAPAPKVL
AEYGFTVENLVKIVRNLK

Sequences:

>Translated_658_residues
MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLHINPAQPNWINRDRFILSAGHGSMLLYALLHLSGFED
VSMDEIKSFRQWGSKTPGHPEFGHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGTDLEAIHAAIETAKASGKPSLIE
VKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALGWDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPE
LAAEVEAIIDGRDPVEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQDDTNRLNR
NIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGLPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
AMPNLNVFRPADARETQAAWYLAVTSEKTPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAV
SAAKELASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAVLGIDTFGASAPAPKVL
AEYGFTVENLVKIVRNLK
>Mature_657_residues
SNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLHINPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDV
SMDEIKSFRQWGSKTPGHPEFGHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEGV
SSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGTDLEAIHAAIETAKASGKPSLIEV
KTVIGYGSPNKQGTNAVHGAPLGADETASTRQALGWDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPEL
AAEVEAIIDGRDPVEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQDDTNRLNRN
IQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGLPVTYVFTHDSIAVGEDGPTHEPVEHLAGLRA
MPNLNVFRPADARETQAAWYLAVTSEKTPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVS
AAKELASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAVLGIDTFGASAPAPKVLA
EYGFTVENLVKIVRNLK

Specific function: Necessary for high-efficiency recombination chromosomal DNA during genetic transformation (PubMed:2361942) [H]

COG id: COG0021

COG function: function code G; Transketolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=595, Percent_Identity=25.8823529411765, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI4507521, Length=595, Percent_Identity=25.8823529411765, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI133778974, Length=586, Percent_Identity=26.4505119453925, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI225637463, Length=513, Percent_Identity=22.8070175438596, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI225637461, Length=513, Percent_Identity=22.8070175438596, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI225637459, Length=513, Percent_Identity=22.8070175438596, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI48994911, Length=664, Percent_Identity=49.8493975903614, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1788808, Length=653, Percent_Identity=49.4640122511485, Blast_Score=612, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI17539652, Length=684, Percent_Identity=25.7309941520468, Blast_Score=153, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325331, Length=663, Percent_Identity=46.3046757164404, Blast_Score=555, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6319593, Length=669, Percent_Identity=43.9461883408072, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI45551847, Length=579, Percent_Identity=26.4248704663212, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45550715, Length=579, Percent_Identity=26.4248704663212, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24645119, Length=550, Percent_Identity=26.3636363636364, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24666278, Length=257, Percent_Identity=33.852140077821, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005478
- InterPro:   IPR020826
- InterPro:   IPR005476
- InterPro:   IPR005474 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 71142; Mature: 71011

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00801 TRANSKETOLASE_1 ; PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLHINPAQPNWINRDRFIL
CCCCCCEEEEEECCHHHCCCCCCCCCEEEECCHHHHHHHHHHEECCCCCCCCCCCCEEEE
SAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEFGHTAGIDATTGPLGQGIS
ECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
TATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEGVSSEAASYAGLQKLDKLVVL
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEE
YDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGTDLEAIHAAIETAKASGKPSLIE
ECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEE
VKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALGWDYEPFEIPEQVYADFKEHVADRG
EEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
ASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDPVEVTPADFPALENGFSQATRNSSQDA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHCCCCHHH
LNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQDDTNRLNRNIQFGVREFAMGTILNGMAL
HHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHH
HGGLRVYGGTFFVFSDYVKAAVRLSALQGLPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
CCCEEEECCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHCC
AMPNLNVFRPADARETQAAWYLAVTSEKTPTALVLTRQNLTVEDGTDFDKVAKGAYVVYE
CCCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCEECCCCCHHHHHCCEEEEEE
NAADFDTILIATGSEVNLAVSAAKELASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAV
CCCCCEEEEEEECCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRVAVEMGASQNWYKYVGLDGAVLGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK
HHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
SNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLHINPAQPNWINRDRFIL
CCCCCEEEEEECCHHHCCCCCCCCCEEEECCHHHHHHHHHHEECCCCCCCCCCCCEEEE
SAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEFGHTAGIDATTGPLGQGIS
ECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCH
TATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEGVSSEAASYAGLQKLDKLVVL
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEE
YDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGTDLEAIHAAIETAKASGKPSLIE
ECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEE
VKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALGWDYEPFEIPEQVYADFKEHVADRG
EEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
ASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDPVEVTPADFPALENGFSQATRNSSQDA
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHCCCCHHH
LNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQDDTNRLNRNIQFGVREFAMGTILNGMAL
HHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHCCHHH
HGGLRVYGGTFFVFSDYVKAAVRLSALQGLPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
CCCEEEECCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCEEECCCCCCCCHHHHHHHCC
AMPNLNVFRPADARETQAAWYLAVTSEKTPTALVLTRQNLTVEDGTDFDKVAKGAYVVYE
CCCCCCEECCCCCCCCCCEEEEEEECCCCCEEEEEEECCCEECCCCCHHHHHCCEEEEEE
NAADFDTILIATGSEVNLAVSAAKELASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAV
CCCCCEEEEEEECCCCEEHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
RRRVAVEMGASQNWYKYVGLDGAVLGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK
HHHHHHHCCCCCCCEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2361942; 11463916; 8248627 [H]